introduction to bioinformatics tuesday, 12 march
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Introduction to Bioinformatics Tuesday, 12 March. Introduction to Bioinformatics Thursday , 28 February. How to choose your research group. Mueser TC et al (2010) Virol J 7:359. Origin of DNA replication. Origin. Circular, dsDNA genome. Origin of DNA replication. Origin. - PowerPoint PPT PresentationTRANSCRIPT
Introduction to BioinformaticsTuesday, 12 March
Introduction to BioinformaticsThursday, 28 February
How to choose your research group
Mueser TC et al (2010) Virol J 7:359
Origin of DNA replicationCircular, dsDNA
genome
Origin
Origin of DNA replicationCircular, dsDNA
genome
Origin
Bidirectionalinitiation
Origin of DNA replicationCircular, dsDNA
genome
Bidirectionalinitiation
Origin
Elongation
Separation
Origin of DNA replicationOrigin
Origin of DNA replicationOrigin
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Origin of DNA replicationOrigin
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FISFactor for Inversion
Stimulation in Phage Mu
IHFIntegration Host
Factor for lysogeny of Phage Lambda
Origin of DNA replicationOrigin
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How to recognize origin of replication?
Origin of DNA replication
Kaguni (2006) Annu Rev Microbiol 60:351-371.
Fuller et al (1984) Cell 38:889-900.
DnaA binding site
Origin of DNA replication
Kaguni (2006) Annu Rev Microbiol 60:351-371.
Fuller et al (1984) Cell 38:889-900.
DnaA binding site
R1 TTATCCACAR2 TTATACACAR3 TTATCCAAAR4 TTATCCACA
Origin of DNA replication
Fuller et al (1984) Cell 38:889-900.
Find it myself
R1 TTATCCACAR2 TTATACACAR3 TTATCCAAAR4 TTATCCACA
Origin of DNA replicationAlgorithm
* Search genome sequence for DnaA-binding sites - TTAT[CA]CACA - One mismatch? - Use MATCHES-OF-PATTERN
* Sort sites by coordinate - Use SORT
* Add context - Loop through sites - JOIN site information with CONTEXT-OF
* Look for clusters - ???
Apply to all phage genomes
* Make problem tangible
Morals of the Story
* Experiment
* Package procedure
Annotation of genes
T4p051
T4p104
T4p120
T4p044
T4p113