introduction to bioinformatics slides
DESCRIPTION
This HIBB presentation provides background information on bases, amino acids, proteins, nucleotides and DNA. The presentation then explains what bioinformatics is, lists some examples, and demonstrates some tools. It demonstrates tools which compare parts of human and chimp genes, and illustrate drug resistance analysis and HIV subtype analysis. It then discusses some ethical and clinical aspects to bioinformatics.TRANSCRIPT
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A brief Introduction to Bioinformatics
Y. SINGH
NELSON R. MANDELA SCHOOL OF MEDICINEDEPARTMENT OF TELEHEALTH
Content licensed under Creative Commons Attribution-Share Alike 3.0 Unported
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Learning Objectives
What is BioinformaticsWhy is it importantExamples of Bioinformatics applicationWhat is SequencingUses of Sequencing
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Building Blocks of DNABases are the building blocks of DNADNA uses four different bases:
Adenine, Guanine, Cytosine & ThymineConnected by 2’-deoxy-ribose-
phosphate backbone
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DNA
Please watch Video One
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Information in DNA is Transferred to RNA & into Proteins
DNA (ACGT on deoxyribose backbone)
RNA (ACGU on ribose backbone)
Proteins (amino acids on peptide backbone)
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Information in RNA Encodes Proteins
Triplets of RNA nucleotides encode 20 amino acids
8 essential amino acids
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DNA Mutates
Mutations in DNA (changes in bases) can changes in amino acids can changes in proteins
Mutations can be:
Inherited: sickle cell disease, cystic fibrosis, susceptibility to some cancers (BRCA: breast cancer)
Acquired: some birth defects, leukemia, HIV resistance
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Definition
Bioinformatics : applied mathematics, informatics, statistics, computer science, artificial intelligence, chemistry, biochemistry etc
to solve biological problems usually on the molecular level
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What can Bioinformatics do
sequence alignment,gene finding, genome assembly, protein structure alignment, protein structure prediction, predict products of gene expression protein-protein interactions,the modeling of evolution.
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What can Bioinformatics do
sequence alignment,gene finding, genome assembly, protein structure alignment, protein structure prediction, predict products of gene expression protein-protein interactions,the modeling of evolution.
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Sequence Alignment
Compare genes within a species
Search genesBLAST
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BIOAFICA:
http://www.bioafrica.net/rega-genotype/html/subtypinghiv.html
STANFORD HIV-DB: http://hivdb.stanford.edu/
Demonstration: Video Two
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FINDING SIMILARITIESHTTP://WWW.EBI.AC.UK/TOOLS/CLUSTALW2/INDEX.HTML
HTTP://WWW.NCBI.NLM.NIH.GOV/SITES/ENTREZ?DB=PROTEIN&CMD=SEARCH
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Implications for clinical informatics
Sequence information in medical recordsNew diagnostic and prognostic information
sourcesEthical considerations
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Thank You