EXP. ,,6,,ENZYMATIC DIGESTION OF FAT BY PANCREATIC

LIPASEIntroduction:

Fats (lipid) : consist of a wide group of compounds that are generally soluble in organic solvents and largely insoluble in water. Chemically, fats are generally triesters of glycerol and fatty acids

A triglyceride consists of a glycerol and three fatty acids. It is the major type of lipid used for energy storage...

Fatty Acids: The lipid building blocks.

Lipases: are enzymes which hydrolyse the triglycerides, releasing fatty acids.

+3H₂O lipase glycerol + f.a

Triglycerides structure:

The hydrolysis process can give all three fatty acids or it can yield one or two F.AS with diglycerides and monoglycerides...

FA1 FA2

FA3 FA2 Lipase + Lipase FA FA1

FA3 FA2

Materials:

1. Fresh whole milk

2. Litmus solution ( or litmus paper) as an indicator.

3. Pancreatin solution (boiled, unboiled).

4. 1% Calcium hydroxide solution.

Procedure:

4ml milk half of A+

+ 10 drops 1ml boiled

Litmus + 3 drops pancreatin so

Ca(OH) 2

+ 1ml unboiled

in water bath (45⁰c) for4-6 hours.

Results:Accumulation of free fatty

acids will reduce the PH.Pink colour with indicatorOdourPrecipitate.