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Santos Jr. CD* 1 ; Dias ACC 1 ; Bonetti AM 1 ; Kerr WE 1 ; Campos TA** 1 1 Laboratório de Genética Molecular - Instituto de Genética e Bioquímica – Universidade Federal de Uberlândia *[email protected]; **[email protected] Keywords: metagenomic, bacteria, endophytes, Ricinus communis, seeds METAGENOMIC ANALYSIS OF ENDOPHYTIC BACTERIAL BIODIVERSITY FROM SEEDS OF Ricinus communis Universidade Federal de Uberlândia The castor bean (Ricinus communis) is a plant of high economic value due its using at the production of the unique oil soluble in alcohol. These characteristics allow its use in more than 400 industrial processes such as: antifreeze production of fuel, plastics, paints, cosmetics and medicines. Despite the widespread technological application of castor bean, little is known about their endophytic microorganisms. The knowledge about this microbiota is fundamental for comprehension of its role in castor bean physiology. In this way, will be possible to know what microbial species are able to survive in this micro-environment with high oil and toxin concentration. This background will collaborate to future studies that aim to what the metabolites and cellular components related and oil production. This study aimed to describe the endophytic bacterial biodiversity present in castor bean seeds by using metagenomic tool. Hitherto we obtained only 33 sequences that were analyzed with 95% confidence threshold. 33.33% of the sequences belonged to Archaea, phylum Crenarcheota (27.27%) and Euryarcheota (6.06%). Another sequences belonged to Eubacteria kingdom: 6.06% to Bacteroides, 15.15% to Proteobacteria, to 18.18% Firmicutes phylum and 27.28% to another. Preliminary results obtained in this work have demonstrated that R. communis seeds microbiota is very diverse. However, sequencing of a bigger number of clones is necessary to access more efficiently the microbiota. Selective PCR with 799F

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METAGENOMIC ANALYSIS OF ENDOPHYTIC BACTERIAL BIODIVERSITY FROM SEEDS OF Ricinus communis. Santos Jr. CD* 1 ; Dias ACC 1 ; Bonetti AM 1 ; Kerr WE 1 ; Campos TA** 1 1 Laboratório de Genética Molecular - Instituto de Genética e Bioquímica – Universidade Federal de Uberlândia - PowerPoint PPT Presentation

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Page 1: INTRODUCTION

Santos Jr. CD*1; Dias ACC1; Bonetti AM1; Kerr WE1; Campos TA**1

1 Laboratório de Genética Molecular - Instituto de Genética e Bioquímica – Universidade Federal de Uberlândia

*[email protected]; **[email protected]

Keywords: metagenomic, bacteria, endophytes, Ricinus communis, seeds

METAGENOMIC ANALYSIS OF ENDOPHYTIC

BACTERIAL BIODIVERSITY FROM SEEDS OF Ricinus

communisUniversidade Federal de Uberlândia

The castor bean (Ricinus communis) is a plant of high economic value due its using at the production of the unique oil soluble in alcohol. These characteristics allow its use in more than 400 industrial processes such as: antifreeze production of fuel, plastics, paints, cosmetics and medicines. Despite the widespread technological application of castor bean, little is known about their endophytic microorganisms. The knowledge about this microbiota is fundamental for comprehension of its role in castor bean physiology. In this way, will be possible to know what microbial species are able to survive in this micro-environment with high oil and toxin concentration. This background will collaborate to future studies that aim to what the metabolites and cellular components related and oil production.

This study aimed to describe the endophytic bacterial biodiversity present in castor bean seeds by using metagenomic tool.

Hitherto we obtained only 33 sequences that were

analyzed with 95% confidence threshold. 33.33% of the

sequences belonged to Archaea, phylum Crenarcheota

(27.27%) and Euryarcheota (6.06%). Another sequences

belonged to Eubacteria kingdom: 6.06% to Bacteroides,

15.15% to Proteobacteria, to 18.18% Firmicutes phylum

and 27.28% to another. Preliminary results obtained in this

work have demonstrated that R. communis seeds

microbiota is very diverse. However, sequencing of a

bigger number of clones is necessary to access more

efficiently the microbiota.

Selective PCR with 799F

http://www.cnpq.br/index.htm
http://www.ufu.br/

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