integrated immunological and metabolic responses of the mammary gland to lps challenge in bovine
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Integrated immunological and metabolic responses of the mammary gland to LPS challenge in bovine. Nissim Silanikove 1* , Adi Rauch-Cohen 1 and Gabriel Leitner 2 - PowerPoint PPT PresentationTRANSCRIPT
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Integrated immunological and metabolic responses of the mammary gland to LPS
challenge in bovineNissim Silanikove1*, Adi Rauch-Cohen1 and Gabriel Leitner2
1 Biology of Lactation Laboratory, Institute of Animal Science, the Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel, 2 Kimaron Veterinary Institute, National Mastitis Center, POB 12, Bet Dagan
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Effect of lactation on metabolism
• The production of milk in intensive producing systems (40 to 70 litters per day in counties such as Israel and the US) encountered for ~ 70-80% of the whole animal metabolizable energy intake
• which is reflected by similar proportion of extraction of the whole body glucose production
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Bacteria (E. Coli) infection may imposes a life threatening situation
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it is well established that Immune response requires considerable proportion of mammalian
energy and metabolite resources
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The general aim of this study was to test hypothesize that the diverse metabolic and immune responses of the mammary gland to LPS challenge describe an integrated mechanism that fights against Gram negative
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METHODOLOGY
Effect of LPS: 6 cows served as control, in second set of six cows one of the front and one rear glands were treated with LPS (10 ml with 10 µg/ ml LPS) while the contra-lateral glands served as running control. The cows milk were sampled at -24h, 0 h (before treatment) and 24, 48 and 76 h post-treatment.
+ -
The data were analyzed for the effect of treatment and
time at a single gland level
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-25 0 25 50 7510
12
14
16
18
20
Milk
Yie
ld (L
/day
)
Time in Relation to LPS Challange (h)
Effect of LPS on milk yield
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-25 0 25 50 754.0
4.2
4.4
4.6
4.8
5.0
5.2
5.4
5.6A
Lact
ose
Con
cent
ratio
n (%
)
Time in Relation to LPS Challange (h)
Effect of LPS on lactose concentration
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-25 0 25 50 7550
100
150
200
250A
Lact
ofer
rin C
once
ntra
tion
(µg/
ml)
Time in Relation to LPS Challange (h)
Effect of LPS lactoferrin concentration
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Effect of LPS on LPO activity, nitrite and nitrotyrosineconcentrations
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Novel findings: Effect of LPS on lactate, malate and citrateconcentrations
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Cytosolic and mitochondrial glycolysis
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Lactic acid metabolism
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Cytosolic formation of malic acid
2 Pyruvic acid + CO2 + ATP Pyruvate carboxylase Oxaloacetic acid 1
+ ADP 2
Oxaloacetic acid + NADH Malic dehydrogenase Malic acid + 1
NAD+ 2
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Low lactose and high lactic acid in broth media affect the growth of pathogenic type of E coli
JA C Q U E S M O N O D From enzymatic adaptation to
allosteric transitionsNobel Lecture, December 11, 1965
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The Main Conclusion
The acute conversion of the epithelial cells metabolism from principally mitochondrial-oxidative
to principally cytosolic (glycolysis) allows the diversion of metabolic resources normally used to synthesize
milk to support the immune system. In turn, the acute increase in the concentration of lactate and malate in
milk and the parallel reduction in lactose concentration are probably effective mean in
restraining invading E Coli growth.