Insulin Production

Ultrafiltration Process

Andrea Adams

1 April, 2004

CHEE 450

Unit Specifications – Givens

Insulin: 5700~5800 Da Polysulfone/Polyethersulfone membrane Rejection Coefficient 100% Concentrate Insulin x 10

Inlet (kg/batch) Oulet (kg/batch)

Acetonitrile 210 14

Insulin 192 191

WFI 5053 342

Theory – Operation Principles

Pressure driven membrane separation process

Membrane configuration is cross-flow

Millipore

Hardware Design

             

Millipore

Visuals

1890-3785 L/day7570-15140 L/day

Filtertech

Membrane

Polysulfone Produced in large quantities so tends to be cheaper

Polyethersulfone PES theoretically has slight advantage over PS More hydrophilic

Cellulose Acetate Low chemical resistance; sensitivity to biodegradation Difficult to clean; must be in constant presence of Cl

Flows

Perry’s

Assumptions Made

Density of inlet stream similar to H2O

1 batch/day 24 h production cycle 316 L mini-batches Flux = 50 L.m-2.h-1

Suppliers

Reverse Osmosis of South Florida (Florida)

Omexell, Inc. (Alabama) Aquafilter, Inc. (Maryland) Southwest Water Systems Co.

(Oklahoma) Uniexcel Agencies & Services

Pvt. Ltd. (India) Nilgiris Chemical Stoneware

Company (India) GEA Filtration (Wisconsin) Culligan International Co.

(Illinois)

Pollard Industries, LLC (Louisiana) Ionics (Massachusetts) Saborn Technologies

(Massachusetts) Berghof Filtration (Germany) Applied Membranes (California) Hydranautics (California) inge AG (Illinois) Koch Membrane Systems (USA) Luxx Ultra-Tech NORIT Membrane Technology BV

(Netherlands) PCI Membrane Systems Ltd. (UK)

Design Considerations

Millipore process specification data entry NMWL (kDa) Width (cm) Stack Design Filtration Area (m2) Height (cm) Length (cm) Filter Material Configuration Diameter (cm)

Sizing

316 L capacity Max. operating pressure: 5 bar Operating conditions: pH 1-14 Dimensions: 97 x 48 x 89 cm Weight: 580 kg Fine Screen: 12-15 L.min-1 per 2.8 m2 cassette Need 20 m2 filtration area, 7 cassettes $147,800

Pall Corporation

Process Alternatives

Spiral Wound oriented Ultrafiltration Hollow Fiber oriented Ultrafiltration Reverse Osmosis

Retains too much Microfiltration

Retains too little Diafiltration