instituto de medicina molecular · 6 imm report 2009.2010 7 imm at a glance 24,97%. core funds fct...
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2009. 2010REPORT
INSTITUTO DE MEDICINA MOLECULARFACULDADE DE MEDICINA DA UNIVERSIDADE DE LISBOA
-INSTITUTO DE MEDICINA MOLECULAR (IMM)FACULDADE DE MEDICINA DA UNIVERSIDADE DE LISBOA
Edifício Egas MonizAv. Prof. Egas Moniz1649-028 LisbonPortugal
-Phone: +351 217 999 411Fax: +351 217 999 [email protected]
-All rights reserved to IMM.No part of this publication may be reproduced or transmittedin any form or by any means, electronic or mechanical, includingphotocopy, recording or any information storage and retrieval system, without prior permission in writing from the publisher.
-
Texts IMM researchers and staff
DesignFormas do Possivel | www.formasdopossivel.com
Edition1000
PhotosIMM staff and Formas do Possível
-May, 2010
2009. 2010REPORT
INSTITUTO DE MEDICINA MOLECULARFACULDADE DE MEDICINA DA UNIVERSIDADE DE LISBOA
IMM REPORT 2009 . 20102 3
CONTENTS
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Barata, João T. - Cancer Biology Unit
Carmo-Fonseca, Maria - Cell Biology Unit
Castanho, Miguel A.R.B. - Physical Biochemistry Unit
Costa, Luís - Clinical and Translational Oncology Research Unit
Constantino Santos, Susana - Angiogenesis Unit
Ferreira, João - Chromatin Biology Unit
Henrique, Domingos - Developmental Biology Unit
Jacinto, António - Tissue Morphogenesis and Repair Unit
Mhlanga, Musa - Gene Expression and Biophysics Unit
Moita, Luís F. - Cell Biology of the Immune System Unit
Saldanha, Carlota - Microvascular Biology and Inflammation Unit
Santos, Nuno C. - Biomembranes Unit
Saúde, Leonor - Embryonic Development of Vertebrates Unit
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Camilo, Maria E. - Nutrition and Metabolism Unit
Figueiredo, Luísa - Parasite Molecular Genetics Unit
Fonseca, João E. - Rheumatology Research Unit
Graça, Luís - Cellular Immunology Unit
Mair, Gunnar - Molecular Parasitology Unit
Mota, Maria - Malaria Unit
Ramirez, M. - Molecular Microbiology and Infection Unit
Silva-Santos, Bruno - Molecular Immunology Unit
Simas, P. - Viral Pathogenesis Unit
Sousa, Ana E. - Clinical Immunology Unit
Veiga-Fernandes, Henrique - Immunobiology Unit
Anes, Elsa - (External) Cell Biology of the Mycobacteria-host Interactions Unit
Gonçalves, João - (External) Retroviruses and Antiviral Research Unit
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IMM AT A GLANCE 06
RESEARCH HIGHLIGHTS 08
STRUCTURE & ORGANIZATION 12
ORGANIGRAM
RESEARCH
FACILITIES AND SERVICES
ABOUT IMM
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64
THE DIRECTORS REPORT 04
FROM BENCH TO CLINIC 68
ADVANCED TRAINING 72
SCIENCE & SOCIETY 76
IMM LIFE 78
1 YEAR IN THE LIFE OF IMM 80
TECHNOLOGY TRANSFER 66
Management Unit
Information Systems
Communication & Training Unit
Lab Management
Bioimaging
Flow Cytometry
Histology Service
Animal Facility
Zebrafish Facility
P3 Facility
01 Cell And Developmental Biology Programme
02 Immunology And Infectious Diseases Programme
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34
Ferro, José - Neurological Clinical Research Unit
Mamede-de-Carvalho - Neuromuscular Unit
Outeiro, Tiago F. - Cell and Molecular Neuroscience Unit
Ribeiro, Joaquim A. - Neurosciences Unit
Rocha, Isabel - Autonomic Nervous System Unit
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03 Neurociences Programme 50
CELL AND DEVELOPMENTAL BIOLOGY PROGRAMME
01
IMMUNOLOGY AND INFECTIOUS DISEASES PROGRAMME
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03NEUROSCIENCES PROGRAMME
CO
NTEN
TS
IMM REPORT 2009 . 20104 5
The IMM was founded in 2001, but its ac-
tivity as an independent institution (“Labo-
ratório Associado”) of the Ministry of Science be-
gan in 2004. This coincided with the installation in
the new Egas Moniz Building.
The initial purpose was to assemble all Laborato-
ries of the Faculty of Medicine of the University
of Lisbon that had been ranked as centers of ex-
cellence by international panels in a single institu-
tion. The advantages seemed to us quite obvious:
to create a critical mass of independent research-
ers, that could lead to a fruitful interdisciplinary
collaboration, to develop common educational
and doctoral programs, and share technical exper-
tise and facilities.
We were able to achieve these goals but, of course,
we were aiming to expand them further, and we
soon realized that we had to follow two main rules:
the periodic critical evaluation by outside experts,
and a stringent policy of hiring only the most
promising young scientists, thus following the rec-
ommendation of specialized panels from outside
of IMM. This policy has been, by our estimation,
remarkably successful. We have presently work-
ing in our Institute about 160 PhD holders and
290 non PhD holders, and 94 PhD students. The
progress in the quality of our research projects
has been outstanding, judging by the output of pa-
pers published in highly ranking journals and the
increase in funding from international sources.
We also appreciate that much was to be gained by
fostering our ties with the medical school and the
university hospital, and we are now full partners
of the new Academic Medical Center. We hope
this will create the opportunity to collaborate in
training programs, both pre-graduate and post-
graduate and, furthermore, develop translational
medicine projects and give stronger support to
the ones already existing, in areas such as clinical
neurosciences, rheumatologic disorders, oncol-
ogy and clinical immunology.
IMM is also a founding member of the Health Clus-
ter Portugal, which was created to strengthen al-
liances between biomedical research institutes,
major health care programs and hospitals and
pharmaceutical and medical device industries in
Portugal.
We are also involved in the new Harvard Medical
THE DIRECTOR’S REPORT
“(...) we expect that everybody gives their best, knowing that the road is sometimes hard and demanding. But there is no other way to achieve excellence in science.”
by J. Lobo Antunes and Maria Carmo-Fonseca
J Lobo Antunes President
Maria Carmo-Fonseca Executive Director
School-Portugal programme, and we have started
a partnership with Inserm-France.
IMM also aims to be the perfect environment for
scientific ideas to grow and turn into products and
technologies that make difference in health care.
To achieve this goal IMM develops ties and strate-
gic plans with companies committed to innovation
and new solutions for the sake of patients. Part-
nerships are established to strengthen positions
in the competitive markets of drug discovery and
diagnostics. These partnerships involve both com-
panies incubated by IMM and external companies.
The high quality of work developed by all research
units of the IMM, either alone or in collaboration,
is generating an increasing level of intellectual
property rights, namely a few patents of invention
with an acknowledged biomedical and/or pharma-
ceutical use. At the same time, IMM researchers
are becoming more aware of questions related to
IP rights or technology transfer.
In 2005 we started GenoMed, a molecular diagno-
sis laboratory, in partnership with Espírito Santo
Saúde, Espírito Santo Capital, Farmindústria and
Fundo de Sindicação de Capital de Risco (IAPMEI).
The volume and sophistication of the tests avail-
able has expanded, and the scientists working in
this laboratory have developed independent re-
search projects.
We are indeed very proud of the achievements
of our scientists, but we are keenly aware of the
challenges and difficulties we face, particularly in
what regards the sustainability of biomedical re-
search in Portugal. We are fully aware that for a
scientist to be productive we need to create the
proper ecology, where new ideas and projects may
flourish, and we have been, we believe, successful
in achieving this. But we will always aim for more
in this ever unended quest.
“It is said that Albert Einstein had written on the wall of his office at Princeton that “not everything that can be counted counts, and not everything that counts can be counted”.
The director’s report
T
THE D
IREC
TOR
’S REPO
RT
IMM REPORT 2009 . 20106 7
IMM at a glance
24,97%. Core Funds FCT9,32%. Ciência Programme Funds18,97%. Faculdade de Medicina da Universidade de Lisboa2,63%. Protocols and donations0,74%. Awards1,03%. OtherResearch Grants:4,12%. International/ Private0,29%. International/Public/Other14,52%. International/Public/EC3,23%. National/Private 3,83%. National/Public/Other16,34% . National/ Public/FCT
IMM AT A GLANCE
RESEARCH PROGRAMMESCell and Developmental BiologyImmunology and Infectious DiseasesNeurosciences
RESEARCH UNITS
EXTERNAL UNITS
COMPANIES
TECHNOLOGICAL FACILITIES
RESEARCHERS IN 2009158 PhD holders
INTERNATIONAL PHD PROGRAMME17 new PhD students in 2009
RESEARCH GRANTS STARTING IN 200919 Public National - FCT10 EC-FP71 Embo6 Private
PEER-REVIEWED SCIENTIFIC PUBLICATIONS IN 2009
13 in journals with > 10 impact factor
ERC LAUREATES
EURYI LAUREATE
HOWARD HUGHES SCHOLAR
3
29
2
225
375
36
187
1
11
IMM
AT A G
LAN
CE
num
ber o
f gra
nts
20
40
30
50
60
FCTOther national ECOther international
EVOLUTION OF GRANT SOURCE
:
IMM FUNDING2009
:Expenditure in 2009
by Funding Source
Total expenditure: 9,450,192 €
0
10
20
30
40
50
60
70
80
0
20
40
60
80
100
120
0
5
10
15
20
25
30
35
40
0
20
40
60
80
100
120
0
10
20
30
40
50
60
70
80
IMM REPORT 2009 . 20106
620
13
35
35
62
5155
20
24
12
29
14
10
23
12
7
6
1518
5
6
10
14 5
2009200820072006200520042003
IMM REPORT 2009 . 20108 9
Research Highlights
PAPERS 2009
:
RESEARCH HIGHLIGHTS
RESEA
RC
H H
IGH
LIGH
TS
Publications JIF > 10Publications JIF 5-10
111
820
619
320
1336
2005
2006
2007
2008
2009
IMPACT FACTOR OF PUBLICATIONS
PER YEAR:
CITATIONS PER YEAR
:
200320022001 2004 2005 2006 2007 2008 2009
187 papers published in peer-reviewed journals:
13 publications in journals with an impact factor higher than 10
36 publications in journals with an impact factor between 5 - 10
Grossman, Z., Meier-Schellersheim, M., Sousa, A.E., Victorino, R.M. and Paul W.E. (2002) CD4+ T-cell de-pletion in HIV infection: are we closer to understand-ing the cause? Nat. Med. 8: 319-321 (Times cited: 230)(Journal IF: 27.553)
Di Fonzo, A., Rohe, C.F., Ferreira, R.J., Chien, H.F., Vacca, L., Stocchi, F., Guedes, L., Fabrizio, E., Man-fredi, M., Vanacore, N., Goldwurm, S., Breedveld, G., Sampaio, C., Meco, G., Barbosa, E., Oostra, B.A. and Bonifati, V. (2005) A frequent LRRK2 gene mutation associated with autosomal dominant Parkinson's disease. Lancet 365: 412-415 (Times cited: 190)(Journal IF: 28.409)
Sousa, A.E., Carneiro, J., Meier-Schellersheim, M., Grossman, Z., and Victorino, R.M. (2002) CD4 T cell depletion is linked directly to immune activation in the pathogenesis of HIV-1 and HIV-2 but only indi-rectly to the viral load. J. Immunol. 169: 3400-3406 (Times cited: 182)(Journal IF: 6.000)
Ribeiro, J.A., Sebastião, A.M. and De Mendonça, A. (2002) Adenosine receptors in the nervous system: pathophysiological implications. Prog. Neurobiol. 68, 377-392 (Times cited: 162)(Journal IF: 9.130)
Duarte, A., Hirashima, M., Benedito, R., Trindade, A., Diniz, P., Bekman, E., Costa, L., Henrique, D. and Rossant, J. (2004) Dosage-sensitive requirement for mouse D114 in artery development. Gene Dev. 18: 2474-2478 (Times cited: 140)(Journal IF: 13.623)
Carmo-Fonseca, M. (2002) The contribution of nu-clear compartmentalization to gene regulation. Cell 108:1-20 (Times cited: 118)(Journal IF: 31.253)
Emre, M., Aarsland, D., Brown, R., Bum, D.J., Duy-ckaerts, C., Mizuno, Y., Broe, G.A., Cummings, J., Dickson, D.W., Gauthier, S., Goldman, J., Goetz, C., Korczyn, A., Lees, A., Levy, R., Litvan, I., McKeith, I., Olanow, W., Poewe, W., Quinn, N., Sampaio, C., Tolosa, E. and Dubois, B. (2007) Clinical diagnostic criteria for dementia associated with Parkinson's disease. Movement. Dis. 22: 1689-1707 (Times cited: 101)(Journal IF: 3.898)
Stamm, S., Riethoven, J.J., Le Texier, V., Gopalakrishnan, C., Kumanduri, V., Tang, Y.S., Barbo-sa-Morais, N.L. and Thanaraj, T.A. (2006) ASD: a bio-informatics resource on alternative splicing Nucleic Acids Res. 34 (Special Issue): D46-D55 (Times cited: 91)(Journal IF: 6.878)
Santos, N.C., Figueira-Coelho, J., Martins-Silva, J. and Saldanha, C. (2003) Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspect. Biochem. Pharmacol. 65: 1035-1041 (Times cited: 89)(Journal IF: 4.838)
Bonifati, V., Rohe, C.F., Breedveld, G.J., Fabrizio, E., De Mari, M., Tassorelli, C., Tavella, A., Marconi, R., Nicholl, D.J., Chien, H.F., Fincati, E., Abbruzzese, G., Marini, P., De Gaetano, A., Horstink, M.W., Maat-Kievit, J.A., Sam-paio, C., Antonini, A., Stocchi, F., Montagna, P., Toni, V., Guidi, M., Dalla Libera, A., Tinazzi, M., De Pandis, F., Fabbrini, G., Goldwurm, S., de Klein, A., Barbosa, E., Lopiano, L., Martignoni, E., Lamberti, P., Vanacore, N., Meco, G. and Oostra, B.A. (2005) Early-onset parkin-sonism associated with PINK1 mutations - Frequency, genotypes, and phenotypes. Neurology 65: 87-95 (Times cited: 79)(Journal IF: 7.043)
10 MOST CITED ARTICLES
2001 -2009:
109160
209440 602 809
1361 1632
9
IMM REPORT 2009 . 201010 11
RESEA
RC
H H
IGH
LIGH
TS
PAPERS WITH RELEVANT MEDIA COVERAGE:
Elsa Abranches, first author of the article, in TV interview.
03.IMM Researchers identify novel viral mechanism associated to the de-velopment of lymphomas
Researchers from the Viral Pathogenesis Unit at IMM identified a viral-mediated molecular mechanism that manipulates the host immune system, leading to per-sistence of herpes virus in the host organism and related to the development of lymphomas. The study involved the collaboration of Portuguese and American re-searchers.
-
02.How stem cells differentiate into neurons in vitro: IMM researchers publish study with roadmap
Researchers at the Developmental Biology Unit of IMM developed and published a study in PLoS One that identifies the molecular mechanisms involved in the dif-ferentiation of embryonic stem cells into neurons. The study, which helps to un-derstand how the embryonic stem cells differentiate to generate neurons, is an important step for the development of new therapies against lesions on the nerv-ous system resulting from trauma or neurodegenerative diseases. The researchers improved the efficiency of a previously known method for in vitro neuron produc-tion and identified the molecules that control the various stages of the process of neuronal differentiation.
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T Lymphocytes: defenders against infection or promoters of autoim-mune diseases?
IMM researchers from the Molecular Immunology Unit, IMM, showed how to iden-tify and control cells populations from the immune system that are responsible for antagonic consequences: defend the organism against infection or develop immune auto-immune disease. The study describes a process of cellular re-education, which may open novel perspectives in cancer immunotherapy and in the combat against autoimmune diseases. Research was developed at IMM, in collaboration with scien-tist from The Neederlands, USA and UK.
-
Ribot J. C., deBarros A., Pang D. J.,
Neves J. F., Peperzak V., Girardi M., Borst
J., Hayday A. C., Pennington D. J. and
Silva-Santos B (2009). CD27 is a thymic
determinant of the balance between IFN-g-
and IL-17-producing gd T cell subsets.
Nature Immunology 10: 427-436.
-
Abranches, E., Silva, M., Pradier, L.,
Schulz, H., Hummel, O., Henrique, D.,
Bekman, E. (2009): Neural Differentiation
of Embryonic Stem Cells in vitro: a Road
Map to Neurogenesis in the Embryo”,
PLos ONE 4(7):e6286.
-
Rodrigues L, Filipe J, Seldon MP,
Anrather J, Soares MP and Simas JP
(2009). Termination of NF-κB activity via
a gherpesvirus protein that assembles an
EC5S ubiquitin-ligase. The EMBO Journal
6;28(9):1283-95. Epub 2009 Mar 26.
-
01.
Bial Award in Clinical Medicine (edition 2008)
Fonseca, J.E., Canhão, H., Santos, M.J., Mourão, A.F., Sousa, E., Caetano-Lopes, J., Moura, R., Weinmann, P., Pereira da Silva, J.A., Cunha-Branco, J., Viana-Queiroz, M.A new vision of inflammatory rheumatic diseases: an example of interaction of cell and molecular biology to the clinic.
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Grunenthal Award in Pain Research (edition 2008)
Tavares, I., Ribeiro, M. and Castanho M.A.R.B.Leading kytorphin derivatives to the central nervous system: experimental validation of a novel analgesic molecule.
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Competition Idea To Product ®, Austin, Texas, USA - 2nd place
Graça, L., Monteiro, M., Cristina, D.Innovative cellular therapy to prevent complications after liver transplantation.
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Calouste Gulbenkian Foundation Programme to Stimulate Creativity and Quality on Scientific Research
Domingues, M.Area: Molecular Recognition.
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Pfizer Award in Clinical Research
Silva-Santos, B.Lymphocyte activation and molecular recognition of malignant cells: implications for cancer immunotherapy.
-
2009 AWARDS
:
Research Highlights
IMM research is regularly highlighted in the media. Scientific articles, rele-vant funding and awards are amongst the most popular references.
IMM REPORT 2009 . 201012 13
IMMUNOLOGY AND INFECTIOUS DISEASES 2010António Freitas (Chair), Institut Pasteur, Paris
Alain Fisher, Necker Hospital Enfants-Malades, Paris
Anne O’Garra, National Institute for Medical Research, London
Philippe Sansonetti, Institut Pasteur, Paris
William Paul, National Institute of Allergy and Infectious Diseases, NIH, Bethesda
CELL AND DEVELOPMENTAL BIOLOGY 2006Philip Ingham (Chair), University of Shefield/Institute of Molecular and Cell Biology, Singapore
Frank Grosveld, Centre for Biomedical Genetics, Erasmus University, Rotterdam
Outi Hovatta, Karolinska Institut, Stockholm
Iain Mattaj, European Molecular Biology Laboratory, Heidelberg
Axel Pries, Department of Physiology, Charité-Berlin, Berlin
NEUROSCIENCES2005Hanna Damasio (Chair), University of Southern California
Charles Warlow, Western General Hospital, Edinburgh
Michael Swash, Royal London Hospital
S. Grillner, Karolinska Institute, Stockholm
K.M. Spyer, University College of London
IMMUNOLOGY AND INFECTIOUS DISEASES 2005António Freitas (Chair), Institut Pasteur, Paris
John Skehel, National Institute for Medical Research, London
George Griffin, St. George’s Medical School, London
Waleria Hryniewicz, National Institute of Public Health, Warsaw
Steffen Gay, University Hospital Zurich
Jeff Platt, Mayo Clinic, USA
IMM IDType private, non-profit research association
Created December 2001
Associate Members Universidade de Lisboa, Faculdade de Medicina da Universidade de Lisboa, Santa Maria Hospital, Fundação Universidade de Lisboa, Associacão para a Investigação e Desenvolvimento da Faculdade de Medicina, Fundação Oriente
History the IMM results from the fusion of 5 former research centres from the Faculdade de Medicina da Universidade de Lisboa.
PREVIOUS SCIENTIFIC ADVISORY
COMMITTEES:
Structure and Organization
STRUCTURE &ORGANIZATIONThe Academic Medical Centre of Lisbon has been created as a consortium of the Santa Maria Hospital, the Lisbon Faculty of Medicine and Instituto de Medicina Molecular with the aim to develop an integrated perspective of Medicine, fostering translational biomedical research from bench to clinic and back to the bench. IMM is a private, non-profit organization which has acquired the status of Laboratory Associated to the National Ministry of Science, Technology and Higher Education. IMM is mainly supported by national public funds and European Union funds. The research expenditure is also supported by funds obtained from peer reviewed competitive grants, private donations and industrial partnerships.
he IMM is managed by a Board of Direc-tors responsible for the accomplishment of
its mission and the annual approval of the plan of activities and budget. The Administration Board, composed by the Board of Directors, the Research Programme Coordinators, and the Directors of the Faculdade de Medicina and Santa Maria Hospital, defines scientific strategies and actions, adminis-trative organization plans and budgetary priorities. The Council of Scientists, composed by all IMM Prin-cipal Investigators, meets at least once per year to discuss the scientific strategies and plan of actions proposed by the Board of Directors. The scientific development of the Institute is monitored by Scien-tific Advisory Committees of international experts who are periodically involved in evaluations of the Institute and its units.The executive arm of the Institute is the Manage-ment Unit, responsible for providing overall adminis-trative support to IMM, including Human Resources, Accounts, Finance, Legal Affairs and Project Man-agement. As funding for research projects comesfrom several national and international sources, IMM management requires the skills of experienced ad-ministrative staff.
T
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ATION
IMM REPORT 2009 . 201014 15
Organigram
ORGANIGRAM
MANAGING DIRECTORMargarida Pinto Gago
EXECUTIVE DIRECTORMaria Carmo Fonseca
BOARD OF DIRECTORSPresident João Lobo AntunesVice-President Joaquim Alexandre RibeiroExecutive Director Maria Carmo Fonseca
RESEARCHCell and Developmental Biology Programme
António JacintoLuís Moita
15 Research Units
Immunology and Infectious Diseases Programm
José Moniz PereiraMário RamirezRui Victorino
11 Research Units
Neurosciences Programme
Ana SebastiãoMamede de Carvalho
5 Research Units
ADVANCED TRAININGPhD Programme
FACILITIESBioimagingFlow CytometryRodentsZebrafishHistologyMicroarraysBiosafety Level 3 Laboratory (P3)
SUPPORT UNITSManagementLab ManagementInformation SystemsCommunication and Funding
“The Administration Board, composed by theBoard of Directors, the Research ProgrammeCoordinators, and the Directors of the Faculdadede Medicina and Santa Maria Hospital, defines scientific strategies and actions, administrative organization plans and budgetary priorities.”
OR
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M
IMM REPORT 2009 . 201014 15
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RESEARCH01 CELL AND DEVELOPMENTAL BIOLOGY PROGRAMME
02 IMMUNOLOGY AND INFECTIOUS DISEASES PROGRAMME
03 NEUROSCIENCES PROGRAMME
IMM REPORT 2009 . 2010
18 1918
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01IMM REPORT 2009 . 2010
21
SELECTED PUBLICATIONS
Cardoso, B.A., Martins, L.R., Santos, C.I., Na-
dler, L.M., Boussiotis, V.A., Cardoso, A.A. and
Barata, J.T. (2009) Interleukin-4 stimulates pro-
liferation and growth of T-cell acute lymphoblas-
tic leukemia cells by activating mTOR signaling.
Leukemia 23: 206-208 (Journal IF: 8.634)
Silva, A. , Yunes, J.A., Cardoso, B.A. , Martins,
L.R., Jotta, P.Y., Abecasis, M., Nowill, A.E.,
Leslie, N.R., Cardoso, A.A. and Barata, J.T.
(2008) PTEN posttranslational inactivation and
hyperactivation of the PI3K/Akt pathway sustain
primary T cell leukemia viability. J. Clin. Invest.
118: 3762-3774 (Journal IF: 16.559)
Barata, J.T., Silva, A., Brandão, J.G., Nadler,
L.M., Cardoso, A.A. and Boussiotis, V.A. (2004)
Activation of PI3K is indispensable for Inter-
leukin 7-mediated viability, proliferation, glucose
use, and growth of T cell acute lymphoblastic
leukemia cells. J. Exp. Med. 200: 659-669 (Jour-
nal IF: 15.463)
Barata, J.T., Boussiotis, V.A., Yunes, J.A., Fer-
rando, A.A., Moreau, L.A., Veiga, J.P., Sallan,
S.E., Look, A.T., Nadler, L.M. and Cardoso,
A.A. (2004) IL-7-dependent human leukemia
T-cell line as a valuable tool for drug discovery
in T-ALL. Blood 103: 1891-1900 (Journal IF:
10.432)
Barata, J.T., Cardoso, A.A., Nadler, L.M. and
Boussiotis, V.A. (2001) Interleukin-7 promotes
survival and cell cycle progression of T-cell acute
lymphoblastic leukemia cells by down-regulating
the cyclin-dependent kinase inhibitor p27kip1.
Blood 98: 1524-1531 (Journal IF: 10.432)
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CANCER BIOLOGY UNIT-
Group Leader
JOÃO TABORDA BARATA
PhD (2003) in Biomedical Sciences at Harvard Medical School, USA, and University of Porto. Post-doctoral research at IMM, Institut Pasteur, France, and Utrecht University, The Netherlands
Research Team
2 Post-doctoral fellows, 5 PhD students, 1 Technician and 1 Trainee
T-ALL cells versus normal control. Constitutive activation of PI3KAkt pathway in tumor cells: Levels of PIP3 in malignant (left) versus normal T cells (right).
IL-7 accelerates leukemia progression in vivo. Mice with (IL-7 WT) or without (IL-7 KO) IL-7 xenotransplanted with T-ALL cells: Tumor burden imaged in live animals.
MAJOR INTERESTS
Cell-autonomous alterations and microenvironmental cues cooperate in the devel-opment of cancer. Our work on lymphoid leukemia aims to dissect the cell-intrinsic and extra-cellular factor-dependent signal transduction mechanisms by which tu-mor cells acquire a selective advantage over their normal counterparts. To do so, we integrate different biochemical, cellular and molecular biology techniques and in vitro and in vivo models of leukemia. The basic and pre-clinical research performed in the lab is translation-oriented and complemented by active ongoing collabora-tions with clinicians. Our ultimate goal is to identify and characterize molecular tar-gets for the development of novel, more selective therapeutic tools against cancer.
he Cell and Developmental Biology programme at IMM covers basic and translational
research themes with a high degree of interdisciplinarity, from the study of single
molecules, to their functions in cellular activity and role in the development of organisms
and in disease.
The molecular mechanisms and principles that underlie the sophisticated organisation and
behaviour of cells, the units of life, are at the core of our research interests. The nucleus
occupies a central role in processing cell information and the study of mechanisms that reg-
ulate gene expression, such as chromatin remodelling and gene splicing, are an important
focus of several groups. We are also very interested in studying several types of interactions
that cells establish with their environment, which range from the physical properties of bi-
omembranes to active processes such as phagocytosis and cell migration.
In order to understand how cells work together to make functional tissues and organisms we
are studying how signalling pathways control embryonic axis specification, cell fate deter-
mination, neural and blood stem cell differentiation, vascular biology and tissue homeosta-
sis. We use a variety of animal models and cell culture assays to investigate these processes.
We also have a strong interest in understanding how cellular malfunction can lead to disease,
thus there are groups studing the molecular mechanisms of cancer with the ultimate aim of
identifying novel molecular markers for diagnosis and targets for therapeutic intervention.
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01CELL AND DEVELOPMENTAL BIOLOGY PROGRAMME
20 IMM REPORT 2009 . 2010
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MAJOR INTERESTS
Have you ever noticed that all cells in every living being have the same kind of fron-tier to separate them from the other cells? Have you ever realized that if we find the right molecules to control or take advantage from this fact, the impact can be tremendous? In the Physical Biochemistry Unit we study molecules of great po-tential to interact with cell membranes: peptides. Some are analgesic, some are antimicrobial (they selectively kill bacteria), some are anti-viral (they block HIV at the entrance of cells), and some are carriers of other molecules but they all share a common characteristic: they have refined ways to interact with lipids. We study those ways and then use our findings to conceive innovative drugs, improve drugs or help on innovative therapeutical approaches, such as gene therapy, for instance. Our ultimate goal is to take our knowledge into the industrial world so that it can be transformed into products that help people live longer and have healthier lives.
SELECTED PUBLICATIONS
Melo, M.N., Ferre, R. and Castanho, M.A.R.B.
(2009) Antimicrobial peptides: linking
partition, activity and high membrane-bound
concentrations. Nat. Rev. Microbiol. 7: 245-250
(Journal IF: 14.31)
Franquelim, H.G., Loura, L.M.S., Santos, N.C.
and Castanho, M.A.R.B. (2008) Sifuvirtide
screens rigid membrane surfaces. Establishment
of a correlation between efficacy and membrane
domain selectivity among HIV fusion inhibitor
peptides. J. Am. Chem. Soc. 130: 6215-6223
(Journal IF: 8.091)
Henriques, S.T., Melo, M.N. and Castanho,
M.A.R.B. (2006) Cell-penetrating peptides and
antimicrobial peptides: how different are they?
Biochem. J. 399: 1-7 (Journal IF: 4.371)
Veiga, A.S., Santos, N.C., Loura, L.M.S.,
Fedorov, A. and Castanho, M.A.R.B. (2004) HIV
fusion inhibitor peptide T-1249 is able to insert
or adsorb to lipidic bilayers. Putative correlation
with improved efficiency, J. Am. Chem. Soc. 126:
14758-14763 (Journal IF: 8.091)
Santos, N.C., Prieto, M. and Castanho, M.A.R.B.
(2003) Quantifying molecular partition into
model systems of biomembranes: an emphasis on
optical spectroscopic methods, Biochim. Biophys.
Acta 1565: 29-35 (Journal IF: 4.180)
-
PHYSICAL BIOCHEMISTRY UNIT-
Group Leader
MIGUEL CASTANHO
PhD (1993) in Molecular Biophysics at Universidade Técnica de LisboaPost-doctoral research at University of Hawaii, USA, and at Rocasolano Institute, Madrid, Spain Full Professor at Faculdade de Medicina da Universidade de Lisboa
Research Team
1 Lecturer, 1 MD-PhD researcher, 4 Post-doctoral fellows, 3 PhD students, 2 MD research students, 4 Undergraduate research students and 1 Technician
Antimicrobial peptides (in red) may permeabilize bacterial lipid membranes in different ways. The most common are depicted in this picture
MAJOR INTERESTS
Recent progress in microarray and high-throughput sequencing data analysis has revealed an unimagined complexity of the human transcriptome. We know now that alternative splicing generates a huge diversity of transcript variants and disruption of splicing regulatory networks is emerging as a major contributor to various dis-eases. There is increasing evidence that a better understanding of splicing mecha-nisms and their physiological regulation is needed for the development of novel intervention strategies for diagnosis, prognosis and treatment of human disease.Making use of a multidisciplinary approach that combines live-cell microscopy, computational modeling, molecular biology, biochemistry and bioinformatics, our group developed expertise to study how the dynamic properties of RNA-protein complexes contribute to post-transcriptional gene regulation. We have discov-ered and are further dissecting a surveillance mechanism that blocks release of abnormal RNAs from the gene template. We have also developed life-cell micro-scopy tools to visualize the dynamics of transcription, spliceosome assembly and splicing, and we are using these methodologies to determine how transcription and splicing are functionally coupled.
SELECTED PUBLICATIONS
Calapez, A., Pereira, H.M., Calado, A., Braga, J.,
Rino, J., Carvalho, C., Tavanez, J.P., Wahle, E.,
Rosa, A.C. and Carmo-Fonseca, M. (2002) The
intranuclear mobility of messenger RNA binding
proteins is ATP-dependent and temperature-
sensitive. J. Cell Biol. 159: 795-805 (Journal IF:
9.120)
Braga, J., Desterro, J.M. and Carmo-Fonseca
M. (2004) Intracellular macromolecular
mobility measured by fluorescence recovery after
photobleaching with confocal laser scanning
microscopes. Mol. Biol. Cell 15: 4749-4760
(Journal IF: 5.558)
Rino, J., Carvalho, T., Braga, J., Desterro, J.M.,
Lührmann, R. and Carmo-Fonseca, M. (2007)
A stochastic view of spliceosome assembly and
recycling in the nucleus. PLoS Comput. Biol. 10:
2019-2031 (Journal IF: 5.895)
Custódio, N., Vivo, M., Antoniou, M. and
Carmo-Fonseca, M. (2007) Splicing and cleavage
independent requirement of RNA polymerase II
CTD for mRNA release from the transcription
site. J. Cell Biol. 179: 199-207 (Journal IF:
9.120)
Grosso, A.R., Gomes, A.Q., Barbosa-Morais,
N.L., Caldeira, S., Thorne, N.P., Grech, G., von
Lindern, M. and Carmo-Fonseca, M. (2008)
Tissue-specific splicing factor gene expression
signatures. Nucleic Acids Res. 36: 4823-4832
(Journal IF: 6.878)
-
CELL BIOLOGY UNIT-
Group Leader
MARIA CARMO-FONSECA
MD (1983) and PhD (1988) in Cell Biology at Faculdade de Me icina da Universidade de Lisboa (FMUL)Post-doctoral research at EMBL in Heidelberg, GermanyProfessor at FMULExecutive Director of the IMM since 2002
Other Principal Investigators
Francisco Enguita
Research Team
5 Lecturers, 3 Post-doctoral fellows, 2 PhD students and 2 Technicians
Spliceosome recruitment to nascent transcripts in the nucleus. Transcriptionally active genes loop out of more condensed territories and occupy the periphery of interchromatin granule clusters (IGCs or nuclear speckles), where transient interactions occur between spliceosomal proteins. Formation of short-lived assembly intermediates likely contributes to increase the local concentration of spliceosomal components, thus enhancing spliceosome assembly on nascent pre-mRNA. (adapted from Rino and Carmo-Fonseca, The spliceosome: a self-organized macromolecular machine in the nucleus? Trends Cell Biol. 19: 357-384, 2009).
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MAJOR INTERESTS
The Clinical and Translational Oncology Research Unit studies mechanisms of inva-sion and progression in solid tumors, particularly in organs such as bone and liver. Tumor invasion and metastasis (tumor spreading to distant organs) are the hall-marks of malignancy, the major causes of death in cancer patients and the main challenge to medicine to improve cure rates in cancer. Our research is performed mostly in human tumor tissues and results are correlated with clinical information. It is our goal to contribute for the identification of tumor cells molecular markers that can correspond to an increased risk factor for metastases occurrence and to identify possible therapeutic targets. New advances in these fields have been achieved and are a good example of productive interaction between basic and clini-cal research. Our research is conducted in collaboration with a well-built network of international researchers, clinicians and laboratories. The research projects ongoing in our Unit have the collaboration of some of the best scientific personalities in the field and generated, already, the interests of drug development department and biomarkers department of some drug compa-nies that have leadership in the oncology field. We also work in close relation with the Oncology fellows from Hospital de Santa Maria, supporting PhD projects of Medical Doctors in training, and encouraging also the participation of other spe-cialists or fellows (surgery, pathology, among others) into the research areas in development at our Unit. We strong believe that collaboration between research clinicians and basic re-search scientists is a key to develop successful projects driven to answer impor-tant clinical questions.
SELECTED PUBLICATIONS
Costa, L. and Major, P.P. (2009) Effect of
bisphosphonates on pain and quality of life in
patients with bone metastases. Nat. Clin. Prac.
Oncol. 6: 163-174 (Journal IF: 9.113)
Lipton, A., Campbell-Baird, C., Harvey, H., Kim,
C., Demers, L. and Costa, L. (2010) Phase I trial
of Zoledronic acid + Imatinib mesylate (Gleevec)
in patients with bone metastases. Am. J. Clin.
Oncol. 33: 75-78 (Journal IF: 1.792)
Casimiro,S., Guise, T.A. and Chirgwin, J. (2009)
The critical role of the bone microenvironment in
cancer metastases. Mol. Cel. Endoc. 310: 71-81
(Journal IF: 3.611)
Vitor, R.F., Esteves, T., Marques, F., Raposinho,
P., Paulo, A., Rodrigues, S., Rueff, J., Casimiro,
S., Costa L. and Santos, I. (2009) (99mTc)-
Tricarbonyl complexes functionalized
with anthracenyl fragments: synthesis,
characterization and evaluation of their
radiotoxic effects in murine melanoma cells.
Cancer Biother. Radiopharm. 24: 1-13 (Journal
IF: 1.318)
Aapro, M., Abrahamsson, P. A., Body, J. J.,
Coleman, R. E., Colomer, R., Costa, L., Crino,
L., Dirix, L., Gnant, M., Gralow, J., Hadji,
P., Hortobagyi, G. N., Jonat, W., Lipton, A.,
Monnier, A., Paterson, A. H. G., Rizzoli, R.,
Saad, F. and Thurlimann, B. (2008) Guidance
on the use of bisphosphonates in solid tumours:
recommendations of an international expert
panel. Ann. Oncol. 19: 420-422 (Journal IF:
4.935)
-
CLINICAL AND TRANSLATIONAL ONCOLOGY RESEARCH UNIT-
Group Leader
LUIS COSTA
MD (1985) at Faculdade de Medicina da Universidade de Lisboa (FMUL)PhD (2002) in Medical Oncology at FMULPost-doctoral research at Santa Maria Hospital and IMM, Lisbon Director of the Oncology Division at the Santa Maria Hospital
Research Team
2 Lectures, 1 Post-doctoral fellow, 3 MD researchers, 2 PhD students and 1 Technician
Breast cancer cell line MDA-MB-231. Immunofluorescence staining with anti-actin antibody (red) and DAPI (nucleus, blue).
Breast cancer metastasis to the spine. Left panel: CT scan shows partial destruction of a vertebral body by cancer; Right panel: the microscopic observation of this lesion obtained by biopsy shows bone destruction by a small group of breast cancer cells (arrow). This case is on study for molecular markers of bone metastases.
MAJOR INTERESTS
Angiogenesis is the formation of new blood vessels from pre-existing ones and is an essential process during development. Nonetheless, it also occurs in adulthood, during wound healing and restoration of blood flow to injured tissues. Angiogen-esis is orchestrated by a variety of pro and anti-angiogenic signals. Their imbalance, promoting either excessive or insufficient angiogenesis, can lead to disease. In can-cer, diabetic eye disease and rheumatoid arthritis, excessive angiogenesis feeds diseased tissue and destroys normal tissue. Conversely, insufficient angiogenesis underlies conditions such as coronary heart disease, stroke and delayed wound healing, where inadequate blood-vessel growth leads to poor circulation and tissue death.The overall goal of the Angiogenesis Unit is to study the molecular and cellular mechanisms that regulate the angiogenic process. In endothelial cells, we described that one of the most important pro-angiogenic factors, the VEGF, and its receptor, KDR, are internalized and have a nuclear localization that is required for endothelial recovery during wound healing. We are now interested in the role of nuclear KDR. Does it function to silence KDR membrane signalling or does it have another spe-cific role? We aim to answer this last question in order to identify new mechanisms that may be targeted in situations of wound healing failure or tumor angiogenesis.Another focus of our unit is to understand the effects of ionizing radiation in the vasculature. Radiotherapy, alone or combined with surgery and chemotherapy, is used as a treatment of choice for tumors. However, several studies showed that ionizing radiation induces the production of pro-angiogenic molecules by the tu-mors that may activate pro-angiogenic responses in other cells. These observa-tions have led us to study the differential response of the vasculature to low doses of ionizing radiation in order to develop novel radio- and chemotherapeutic strate-gies to better control of tumor re-growth and metastasis.
SELECTED PUBLICATIONS
Constantino Rosa Santos, S., Miguel, C.,
Domingues, I., Calado, A., Zhu, Z., Wu, Y. and
Dias, S. (2007) VEGF and VEGFR-2 (KDR)
internalization is required for endothelial
recovery during wound healing. Exp. Cell Res.
313: 1561-1574 (Journal IF: 3.948)
Constantino Rosa Santos, S. and Dias, S. (2004)
Internal and external autocrine VEGF/KDR loops
regulate survival of subsets of acute leukemia
through distinct signaling pathways. Blood 103:
3883-3889 (Journal IF: 10.9)
Constantino Rosa Santos, S., Lacronique,
V., Bouchaert, I., Monni, R., Bernard, O.,
Gisselbrecht, S. and Gouilleux, F. (2001)
Constitutively active STAT5 variants induce
growth and survival of hematopoietic cells
through a PI 3-Kinase/AKT dependent pathway.
Oncogene 20: 2080-2090 (Journal IF: 7.216)
Monni, R., Constantino Rosa Santos, S.,
Mauchauffe, M., Berger, R., Ghysdael, J.,
Gouilleux, F., Gisselbrecht, S., Bernard, O. and
Pernard-Lacronique, V. (2001) The TEL-Jak2
oncoprotein induces Socs1 expression and altered
cytokine response in BaF3 cells. Oncogene 20:
849-858 (Journal IF: 7.216)
Constantino Rosa Santos, S., Dumon, S.,
Mayeux, P., Gisselbrecht, S. and Gouilleux,
F. (2000) Cooperation between STAT5 and
phosphatidylinositol 3-kinase in the IL-3
dependent survival of the bone marrow derived
cell line. Oncogene 19: 1164-1172 (Journal IF:
7.216)
-
ANGIOGENESIS UNIT-
Group Leader
SUSANA CONSTANTINO SANTOS
PhD (2001) in Bases Fondamentales de l’Oncogénèse at Université de Paris 7, France Post-doctoral research at Instituto Português de Oncologia, LisbonAssistant Professor at the Faculdade de Medicina da Universidade de Lisboa Group leader at IMM since 2008
Research Team
1 Post-doctoral fellow, 2 PhD students and 1 Technician
Live imaging: Zebrafish caudal fin vaculature (by Inês Vala)
Bioimaging: Lung metastases were quantified by bioimaging (By Inês Vala)
Live cell imaging: Human endothelial cells overexpressing the GFP-KDR fusion protein (by Inês Domingues)
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MAJOR INTERESTS
In vertebrates, neurogenesis starts very early during embryonic life and the major-ity of neurons is produced before birth. A conserved genetic circuitry, comprising the action of both proneural and neurogenic genes, is known to control the pro-duction of neurons during embryonic development. Our work aims to address how this circuitry functions to regulate the establishment of neural precursors in verte-brate embryos and how they give rise to the multitude of neurons that compose the adult CNS. We are particularly interested in dissecting the function of the Delta/Notch signaling pathway during neurogenesis in the developing spinal cord and neural retina, using transgenic approaches in mice embryos. We have also devel-oped methods to use Embryonic Stem cells for ex vivo production of neurons and are interested in dissecting the mechanisms responsible for the emergence and maintenance of stem cells in the embryo. We believe that a better knowledge about these fundamental mechanisms is a pre-requisite for the development of cellular replacement therapies to treat neurodegenerative diseases, with a significant im-pact on human health.
SELECTED PUBLICATIONS
Rocha, S.F., Lopes, S.S., Gossler, A. and Henrique,
D. (2009) Dll1 and Dll4 function sequentially
in the retina and pV2 domain of the spinal cord
to regulate neurogenesis and create cell diversity.
Dev. Biol. 328: 54-65 (Journal IF: 4.416)
Abranches, E., Silva, M., Pradier, L., Schulz,
H., Hummel, O., Henrique, D. and Bekman, E.
(2009) Neural differentiation of embryonic stem
cells in vitro: a road map to neurogenesis in the
embryo. PLoS ONE 4: e6286 (Journal IF: NA)
Diogo, M.M., Henrique, D. and Cabral, J.M.S.
(2007) Optimization and integration of expansion
and neural commitment of mouse embryonic stem
cells. Biotechnol. Appl. Biochem. 49: 105-112
(Journal IF: 1.288)
Afonso, C. and Henrique, D. (2006) PAR3 acts as
a molecular organizer to define the apical domain
of chick neuroepithelial cells. J. Cell Science 119:
4293-4304 (Journal IF: 6.247)
Fior, R. and Henrique, D. (2005) A novel hes5/
hes6 circuitry of negative regulation controls
Notch activity during neurogenesis. Dev. Biol.
281: 318-333 (Journal IF: 4.416)
Fior, R. and Henrique, D. (2009) “Notch-Off”:
a perspective on the termination of Notch signal-
ling. Int. J. Dev. Biol. 53: 1379-1384 (Journal IF:
2.359)
-
DEVELOPMENTAL BIOLOGY UNIT-
Group Leader
DOMINGOS HENRIQUE
PhD (1991) at Universidade de LisboaPost-doctoral research at NIMR and ICRF, UK and Institut d’Embryologie Cellulaire et Moléculaire, FranceInvestigator at Faculdade de Medicina da Universidade de Lisboa
Research Team
4 Post-doctoral fellows, 2 PhD students, 1 Lab Manager and 1 Technician
Neural Rosettes in culture containing ES-derived neuroepithelial progenitors (labelled in green) engaged on neuronal production (neurons are labelled red).
A section of a region of the embryonic CNS of a chicken embryo, containing the developing dorsal root ganglia where neurons (labelled in green) of the PNS are generated.
Differentiated neurons (green) generated in vitro from ES cells, showing extended neuronal processes (labelled in red).
Pictures of the mouse embryonic retina (a,b) and spinal cord (c,d), showing undifferentiated progenitors and maturing neurons.
A section of the neural tube of a mouse embryo at E12.5, where the activity of the Notch target gene NRARP is visualized in red. This gene is active in neuroepithelial progenitors located close to the lumen of the neural epithelium.
MAJOR INTERESTS
The main objective is to investigate the role of chromatin structure and topology in genome function and propagation. We are particularly interested in disclosing how topoisomerases, the molecules that control the topology of DNA/chromatin inside the cell nucleus, are targeted to their sites of activity and influence nuclear phenomena namely the repair of DNA lesions and the biogenesis of specific types of chromatin. We also wish to investigate to what extent post-translational modi-fications of topoisomerase II influence chromosome segregation during mitosis. Finally, we wish to contribute to the understanding of how cytotoxic drugs that tar-get topoisomerases exert their effects in cancer cells and which cellular factors af-fect chemoresistance. In this context, we shall address how chromatin and splicing regulators affect the accelerated cellular senescence programme that is triggered in cancer cells by inhibitors of topoisomerases.
SELECTED PUBLICATIONS
Agostinho, M., Santos, V., Ferreira, F., Costa,
R., Cardoso, J., Pinheiro, I., Rino, J., Jaffray, E.,
Hay, R.T. and Ferreira, J. (2008) Conjugation
of human topoisomerase 2 with SUMO-2/-3 in
response to topoisomerase inhibitors: cell cycle
stage and chromosome domain specificity. Cancer
Res. 68: 2409-2418 (Journal IF: 7.514)
Agostinho, M., Rino, J., Braga, J., Ferreira, F.,
Steffensen, S. and Ferreira, J. (2004) Human
topoisomerase IIα: targeting to subchromosomal
sites of activity during interphase and mitosis.
Mol. Biol. Cell 15: 2388-2400 (Journal IF: 5.558)
Poot, R., Bozhenok, L., van den Berg, D.L.C.,
Steffensen, S., Ferreira, F., Grimaldi, M., Ferreira,
J. and Varga-Weisz, P. (2004) The Williams
Syndrome transcription factor interacts with
PCNA to target chromatin remodeling by ISWI
to replication foci. Nat. Cell Biol. 6: 1236- 1244
(Journal IF: 17.774)
-
CHROMATIN BIOLOGY UNIT-
Group Leader
JOÃO FERREIRA
MD (1983) and PhD (1999) in Cell Biology at Faculdade de Medicina da Universidade de Lisboa (FMUL)Medical Residency in Dermatology, Curry Cabral Hospital, Lisbon (1987-90)Associate Professor at FMUL
Research Team
1 Post-doctoral fellow, 1 PhD student and 1 Trainee
Nucleus (blue signal) of a cell exposed to a topoisomerase-specific drug. Sites of damaged DNA (green signal) concentrate DNA repair proteins (red signal), as best seen in the merged (right) image.
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MAJOR INTERESTS
In biology several important processes occur at spatial dimensions currently be-yond the reaches of conventional light microscopy. Our laboratory is concerned with those biological questions related to gene expression. As such the study of RNA transcription, metabolism and transport as well as its relation with dynamic compartmentalized multi-molecular complexes in the eukaryotic cell nucleus re-main well outside the resolution of most optical microscopy techniques. The direct observation of these events at the single molecule level, is currently the subject of intense research. Intrinsic to RNA transcription are modifications to the nuclear architecture and the dynamic repositioning of chromosomal loci, as well as the in-terplay of several ribonucleic proteins (RNPs). These events remain opaque at the single RNA transcript level, and questions such as the timing and spatial position of interactions of these RNPs with RNA, the number of genes targeted in the tran-scriptional process at a single spatial location, the splicing and the export of RNA, remain unresolved. Understanding and visualizing these processes has implica-tions not only for our understanding of basic biology, but extends to mechanisms in development and disease. Through the use of novel nucleic acid based probes (molecular beacons) for the detection of mRNA in living cells, we have recently extended these approaches to the imaging of gene expression and RNA transport in living cells. We are currently extending our ability to do single molecule biology through the development of super-resolution imaging techniques.
SELECTED PUBLICATIONS
Henriques, R., Mhlanga, M.M. (2009) PALM and
STORM: What hides beyond the Rayleigh limit?
Biotechnol. J. 4: 846 – 857 (Journal IF: NA)
Mhlanga, M.M., Bratu, D.P., Genovesio, A.,
Rybarska, A., Chenouard, N., Nehrbass,U. and
Olivo-Marin, J.C. (2009) “In vivo” colocalisation
of oskar mRNA and trans-acting proteins revealed
by quantitative imaging of the Drosophila oocyte.
PLoS One 4: e6241 (Journal IF: NA)
Mhlanga, M.M. and Tyagi, S. (2006) Using tRNA-
linked molecular beacons to image cytoplasmic
mRNAs in live cells. Nat. Protoc. 1: 1392-1398
(Journal IF: 4.170)
Mhlanga, M.M., Vargas, D.Y., Fung, C.W.,
Kramer, F.R. and Tyagi, S. (2005) tRNA-linked
molecular beacons for imaging mRNAs in the
cytoplasm of living cells. Nucleic Acids Res. 33:
1902-1912 (Journal IF: 6.878)
Bratu, D.P., Cha, B.J., Mhlanga, M.M., Kramer,
F.R. and Tyagi, S. (2003) Visualizing the
distribution and transport of mRNAs in living
cells. Proc. Natl. Acad. Sci. USA 100: 13308-
13313 (Journal IF: 9.380)
Mhlanga, M.M. and Malmberg, L. (2001) Using
molecular beacons to detect single nucleotide
polymorphisms with real-time PCR. Methods 25:
463-471 (Journal IF: 3.291)
Kostrikis, L.G., Tyagi, S., Mhlanga, M.M., Ho,
D.D. and Kramer, F.R. (1998) Spectral genotyping
of human alleles. Science 279: 1228- 1229 (Journal
IF: 28.103)
-
GENE EXPRESSION AND BIOPHYSICS UNIT-
Group Leader
MUSA MHLANGA
PhD (2003) in Cell Biology at the New York University School of Medicine, USAPostdoctoral research at Institut Pasteur, FranceGroup Leader at IMM since 2008
Research Team
1 PhD student and 1 Trainee
MAJOR INTERESTS
Epithelia have the essential role of acting as a barrier that protects living organ-isms and its organs from the surrounding environment. It is crucial for epithelial tissues to have robust ways of maintaining their integrity despite the frequent damage caused by injury, inflammation and normal cell turnover. Our objective is to understand molecular and cellular processes that regulate epithelial resealing, a conserved fundamental repair mechanism that acts in several types of simple epi-thelia, both in embryos and in adults, and across species. The organism of choice has been the fruit fly (Drosophila melanogaster) due to the amenability of this model system to genetic manipulation and live imaging analysis. We are also using flies to investigate the mechanisms that control the recruitment of macrophage-like blood cells to wound sites to clear damaged cells and to study a natural occurring mor-phogenetic movement, dorsal closure, that has striking similarities to wound repair. Recently we have started using Zebrafish to translate our Drosophila findings to a vertebrate model and to explore more complex tissue repair mechanisms, such as epimorphic regeneration.
SELECTED PUBLICATIONS
Campos, I., Geiger, J.A., Santos, A.C., Carlos,
V. and Jacinto, A. (2010) Genetic screen in
Drosophila melanogaster uncovers a novel set
of genes required for embryonic epithelial repair.
Genetics 184: 129-140 (Journal IF: 4.002)
Wood, W. and Jacinto, A. (2007) Drosophila
melanogaster embryonic haemocytes: masters of
multitasking. Nat. Rev. Mol. Cell Biol. 8: 542-551
(Journal IF: 35.423)
Simões, S., Denholm, B., Azevedo, D., Sotillos, S.,
Martin, P., Skaer, H., Castelli-Gair Hombria, J.
and Jacinto, A. (2006). Compartmentalization of
Rho regulators directs cell invagination during tis-
sue morphogenesis. Development 133: 4257-4267
(Journal IF: 6.812)
Wood, W., Faria, C. and Jacinto A. (2006) Dis-
tinct mechanisms regulate hemocyte chemotaxis
during development and wound healing in Dro-
sophila. J. Cell Biol 174: 405-416.
Wood, W., Jacinto, A., Grose, R., Gale, J.,
Wilson, C. and Martin, P. (2002) Wound healing
recapitulates morphogenesis , co-ordinated
regulation of actin cytoskeletal elements by small
GTPases during repair of epithelial wounds in
Drosophila embryos. Nat. Cell Biol. 4: 907-912
(Journal IF: 17.774) 4: 907-912.
-
TISSUE MORPHOGENESIS AND REPAIR UNIT-
Group Leader
ANTÓNIO JACINTO
PhD (1999) in Developmental Biology at the Imperial College of Science and Technology and Medicine, University of London, UKPost-doctoral research at University College London, UK Group Leader at Instituto Gulbenkian Ciência (2001-04) and at IMM since 2004 Assistant Professor at Faculdade de Medicina da Universidade de Lisboa Awarded a European Research Council Starting Grant Awarded a Human Frontier Science Program Grant
Other Principal Investigator
Soren Prag
Research Team
9 Post-doctoral fellows, 6 PhD students, 2 researchers, 1 Master student and 2 Technicians
This sequence of images shows a region of a Drosophila embryonic epithelium where the outlines of the cells are marked with GFP. A wound of about 20 micrometers (dashed line) induced with a laser closes in about 1 hour. The molecular mechanisms that regulate this process are largely unknown and are under intense study in our laboratory.
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MAJOR INTERESTS
The vascular wall stays at the cornerstone of inflammation controlling the partici-pation and recruitment of circulating blood cells. Our research is focused on the molecular and cellular processes occurring at blood-vessel wall interface in the context of inflammation. To address this, we conjugate molecular and cellular biol-ogy approaches with microcirculation studies, such as high-speed intravital micro-scopy imaging of in vivo animal models.By using rat, mice and zebrafish models, our research aims at understanding the mechanisms that govern leukocyte recruitment and cell-cell interaction in inflam-mation. We are currently addressing the role of specific inflammatory mediators, in modulating blood-vessel wall interaction in inflammation and the mechanisms that regulate neutrophil-induced monocyte recruitment during the inflammatory response. We research on the role of erythrocyte as a pro-active blood component on inflammation.Based on these studies, we collaborate at developing theoretical simulation models of phenomena occurring at the level of leukocyte-vascular wall interface, particu-larly related to inflammatory processes.We are also collaborating with a start-up biotech company in the development of new methodologies for the determination of hemorheologic parameters.
SELECTED PUBLICATIONS
Carvalho, F.A., Martins-Silva, J. and Saldanha,
C. (2004) Amperometric measurement of nitric
oxide in erythrocytes. Biosens. Bioelectron. 20:
505-508 (Journal IF: 5.143)
Artoli, A.M., Sequeira, A., Silva-Herdade, A.S.
and Saldanha, C. (2007) Leukocytes rolling and
recruitment by endothelial cells: hemorheologi-
cal experiments and numerical simulations. J.
Biomech. 40: 3493-3502 (Journal IF: 3.520)
Santos, S.C.R., Vala, I., Miguel, C., Barata, J.P.,
Garção, P., Agostinho, P., Mendes, M., Coelho,
A.V., Calado, A., Oliveira, C.R., Martins e
Silva, J. and Saldanha, C. (2007) Expression
and subcellular localization of a novel nuclear
acetylcholinesterase protein. J. Biol. Chem. 282:
25597-25603 (Journal IF: 5.520)
Lund, E., Guttinger, S., Calado, A., Dahlberg,
J.E. and Kutay, U. (2004) Nuclear export of mi-
croRNA precursors. Science 303: 95-98 (Journal
IF: 28.103)
Rosário, H.S., Waldo, S.W., Becker, S.A.,
Schmid-Schönbein, G.W. (2004) Pancreatic
trypsin increases matrix metalloproteinase-9
accumulation and activation during acute
intestinal ischemia-reperfusion in the rat. Am. J.
Pathol. 164: 1707-1716 (Journal IF: 5.697)
-
MICROVASCULAR BIOLOGY AND INFLAMMATION UNIT-
Group Leader
CARLOTA SALDANHA
PhD (1986) in Biochemistry at Universidade Nova de LisboaMaster (2000) in Medical Education joint degree at University of Wales and Faculdade de Medicina da Universidade de Lisboa (FMUL)Associated Professor with Habilitation at FMUL
Research Team
2 Lecturers, 1 Teaching assistant, 2 PhD students, 1 Trainee and 1 Technician
Neutrophils labeled with DAPI (blue), fibrinogen (green) and integrin Mac-1 (red).
Mesenteric venule in animal model (rat) for observation of leukocyte-vascular wall interaction.
Mathematical simulation of a leukocyte approaching the endothelial wall.
Neutrophils (green) in a 3 day pos-fertilization transgenic mpx::egfp zebrafish.
MAJOR INTERESTS
The study of host-pathogen interactions is critical for the understanding of the outcome of immune responses (tolerance vs. immunity) and has the potential to lead the way for the design of novel effective therapeutic strategies. As a novel approach to understand how the physical and biochemical nature of particulate antigens influences their uptake and fate in Antigen Presenting Cells (APCs), we are studying the internalization, traffic and processing of ‘synthetic pathogens’ - model particles with distinct, well-defined physical and biochemical properties. We are comparing how signals from host opsonins or pathogen structure/composition itself modulate phagocytosis and subsequent immunity in the context of a single well-defined particle platform- to allow individual parameters of pathogen struc-ture/composition to be varied and their effects compared in a single model sys-tem. We hope to better understand how pathogen structure and chemistry dictates signaling, intracellular traffic, antigen processing, immune responses and pathogen survival or elimination.In addition to the studies on pathogen structure and composition (Fig. 1), our Unit uses RNA interference (RNAi) to identify genes with a previous uncharacterized role in several processes (Fig.2, for example) that are relevant for immune re-sponses. We are currently focusing on two main questions: (1) what is the role of alternative splicing in the regulation of the immune response? And, (2) what are the molecular mechanisms of antigen cross-presentation?Since RNA interference (RNAi) was discovered to work in mammalian cells, this ge-netic manipulation technique has been hailed as a revolutionary new approach to basic biological research and drug development and discovery. RNAi is expected to provide critical insights into the mechanisms underlying human disease and accel-erating development of treatments for cancer, AIDS and a host of other disorders.
SELECTED PUBLICATIONS
Ostrowski, M., Carmo, N.B., Krumeich, S.,
Fanget, I., Raposo, G., Savina, A., Moita, C.F.,
Schauer, K., Hume, A.N., Freitas, R.P., Goud, B.,
Benaroch, P., Hacohen, N., Fukuda, M., Desnos,
C., Seabra, M.C., Darchen, F., Amigorena, S.,
Moita, L.F.* and Thery, C.* (2010) Rab27
controls constitutive exosome secretion. Nat. Cell
Biol. 12: 19-30 *Joint corresponding authors
(Journal IF: 17.774)
Robinson, M., Osorio, F., Rosas, R., Freitas,
R., Schweighoffer, E., Groß, O., Verbeek, J.S.,
Ruland, J., Tybulewicz, V., Brown, G.D., Moita,
L.F., Taylor, P.R. and Sousa, C.R (2009) Dectin-2
is a Syk-coupled pattern recognition receptor
crucial for Th17 responses to fungal infection. J.
Exp. Med. 206: 2037-2051 (Journal IF: 15.463)
Savina, A., Peres, A., Cebrian, I., Carmo, N.,
Moita, C., Hacohen, N., Moita, L.F., and
Amigorena, S. (2009) Rac2 controls phagosomal
alkalinization and crosspresentation selectively
in CD8+ dendritic cells. Immunity 30: 544-555
(Journal IF: 20.579)
Oberdoerffer, S., Moita, L.F., Neems, D., Freitas,
R.P., Hacohen, N. and Rao, A. (2008) Regulation
of CD45 alternative splicing by heterogeneous
ribonucleoprotein, hnRNPLL. Science 321: 686-
691 (Journal IF: 28.103)
Moita, L.F.*, Wang-Sattler, R., Michel, K.,
Zimmermann, T., Blandin, S., Levashina, E.A.
and Kafatos, F.C. (2005) In vivo phagocytic
screen in A. gambiae: new players and conserved
pathways of engulfment. Immunity 23: 65-73
*Corresponding author (Journal IF: 20.579)
-
CELL BIOLOGY OF THE IMMUNE SYSTEM UNIT-
Group Leader
LUÍS FERREIRA MOITA
MD (1997) at Universidade de LisboaPhD (2003) in Cell and Molecular Biology at EMBL in Heidelberg, Germany Post-doctoral research at Harvard Medical School and MIT, USAAssistant Professor at Faculdade de Medicina da Universidade de LisboaAwardee of the Human Frontier Science Program
Other Principal Investigator
Nina Matova
Research Team
4 Post-doctoral fellows, 1 PhD student, 1 MD-PhD Student, 2 MD researchers and 1 Technician
IMM REPORT 2009 . 201032
01 Cell and Developmental Biology Programme
33
MAJOR INTERESTS
How does a single cell, the fertilized egg, divides into millions of cells that will or-ganize themselves into the tissues and organs that compose the human body is a fascinating question in Biology. Our long-term research goal is to understand the genes, the molecules and the mechanisms that orchestrate the assignment of dif-ferent cell types into the correct positions within the developing embryo. Once we uncover these developmental pathways, we will be able to fully understand inher-ited human defects and find ways of repairing and replacing tissues.Our Unit addresses several questions. How is the left-right asymmetric localiza-tion, structure and function of vital organs like the heart, the liver and the brain achieved? How is this coordinated? The different vertebrae that constitute the vertebral column and the muscles that move the skeleton are not formed all at the same time, but rather in a periodic and sequential manner under the control of a molecular clock. When and how is this molecular clock started in the embryo? Is this molecular clock used to control the progressive laid down of skeletal elements that will form a fully functional fin? Is the molecular clock reused to control the timing of repair after organ injury or amputation?We use zebrafish, chick and mouse embryos as animal models. They offer com-plementary embryological, molecular and genetic approaches, allowing a more comprehensive understanding of the mechanisms of development. The large and flat chick embryo allows position specific surgical manipulations. The genetics of mouse and zebrafish is a powerful tool to analyse the effects of loss of gene func-tion and interactions among genes. In addition, the transparency of the zebrafish embryo allows the visualization of developmental processes in the living embryo using high-resolution microscopes.
SELECTED PUBLICATIONS
Saúde, L., Lourenço, R., Gonçalves, A.
and Palmeirim, I. (2005) terra is a left-
right asymmetry gene required for left-right
synchronization of the segmentation clock. Nat.
Cell Biol. 7: 918-992 (Journal IF: 17.774)
Coutinho, P., Parsons, M.J., Thomas, K.A., Hirst,
E.M., Saúde, L., Campos, I., Williams, P.H. and
Stemple D.L. (2004) Differential requirements
for COPI transport during vertebrate early
development. Dev. Cell 7: 547-558 (Journal IF:
12.882)
Feldman, B., Concha, M.L., Saúde, L., Parsons,
M.J., Adams, R.J., Wilson, S.W. and Stemple
D.L. (2002) Lefty antagonism of Squint is
essential for normal gastrulation. Curr. Biol. 12:
2129-2135 (Journal IF: 10.777)
Saúde, L., Woolley, K., Martin, P., Driever,
W. and Stemple D.L. (2000) Axis-inducing
activities and cell fates of the zebrafish organizer.
Development 127: 3407-3417 (Journal IF: 6.812)
Heisenberg, C-P., Tada, M., Rauch, G-J.,
Saúde, L., Concha, M.L., Geisler, R., Stemple,
D.L., Smith, J.C. and Wilson S.W. (2000)
Silberblick/Wnt11 mediates convergent extension
movements during zebrafish gastrulation. Nature
405: 76-81 (Journal IF: 31.434)
-
EMBRYONIC DEVELOPMENT OF VERTEBRATES UNIT-
Group Leader
LEONOR SAÚDE
PhD (2001) in Developmental Biology at University College London, UK Post-doctoral research at Instituto Gulbenkian de Ciência (IGC)Group Leader at IGC (2005-07) and at IMM since 2008Assistant Professor at Faculdade de Medicina da Universidade de Lisboa
Research Team
3 Post-Doctoral fellows, 3 PhD students, 1 Master student, 1 Technician
A mouse embryo with 9 days of development expresses a gene named terra in the somites.
A zebrafish embryo with 12 hours of development. In the tail region, cilia can be spotted with a green labelling inside an organ called Kupffer’s vesicle. These cilia control the positioning of the internal organs inside the body cavities.
MAJOR INTERESTS
Biological membranes serve not only as biological boundaries but also as dynamic structures essential for several cellular events, both on health and disease. The scope of the Unit is the study of biochemical and biophysical processes occurring at the level of the membranes of human cells and of their viral and bacterial path-ogens, even without the involvement of membrane receptors. The Unit is mainly focused on the two steps of the life cycle of enveloped viruses (mainly HIV and dengue virus) that involve biomembranes: the entry of the virus in a target cell and the release of new virus. The questions that we try to answer are related to specific lipid recruitment during viral assembly, virus-target cell interactions conditioning by viral lipid composition and, the role of lipid rafts (heterogeneous membrane are-as enriched in specific components) on the virus life cycle and on the action of some anti-HIV drugs. The answering of some of these questions may reveal specific in-teractions, show how they can be modulated and, hopefully, identify new targets for antiretroviral therapy. Adding to this main focus, we are also involved in col-laborative projects on: i) the evaluation of the activity of microbicides that bind to a specific component of the membranes of some bacteria; ii) the study of the bind-ing of a plasma protein to red blood cells and its relevance as cardiovascular risk factor; iii) the evaluation of a potential marker in Alzheimer disease; and, iv) on the Nanomedicine / Nanotechnology area, the structural characterization of metal na-noparticles conjugated with proteins for biomedical applications (e.g., biosensors).
SELECTED PUBLICATIONS
Domingues, M.M., Castanho, M.A.R.B.
and Santos, N.C. (2009) rBPI21 promotes
lipopolysaccharide aggregation and exerts
itsantimicrobial effects by (hemi)fusion of PG
containing membranes. PLoS One 4: e8385
(Journal IF: NA)
Franquelim, H.G., Loura, L.M.S., Santos, N.C.
and Castanho, M.A.R.B. (2008) Sifuvirtide
screens rigid membrane surfaces. Establishment
of a correlation between efficacy and membrane
domain selectivity among HIV fusion inhibitor
peptides. J. Am. Chem. Soc. 130: 6215-6223
(Journal IF: 8.091)
Santiago, P.S., Moura, F., Moreira, L.M.,
Domingues, M.M., Santos, N.C. and Tabak,
M. (2008) Dynamic light scattering and
optical absorption spectroscopy study of pH
and temperature stabilities of the extracellular
hemoglobin of Glossoscolex paulistus. Biophys. J.
94: 2228-2240 (Journal IF: 4.683)
Veiga, A.S., Santos, N.C., Loura, L.M.S., Fedorov,
A. and Castanho, M.A.R.B. (2004) HIV fusion
inhibitor peptide T-1249 is able to insert or adsorb
to lipidic bilayers. Putative correlation with
improved efficiency. J. Am. Chem. Soc. 126:14758-
14763 (Journal IF: 8.091)
Santos, N.C., Prieto, M. and Castanho, M.A.R.B.
(2003) Quantifying molecular partition into model
systems of biomembranes: an emphasis on optical
spectroscopic methods. Biochim. Biophys. Acta
1612: 123-135 (Journal IF:4.180)
-
BIOMEMBRANES UNIT-
Group Leader
NUNO C. SANTOS
PhD (1999) at Universidade de LisboaResearcher at Universidade Técnica de Lisboa and at University of California, Santa Barbara, USA Assistant Professor at Faculdade de Medicina da Universidade de Lisboa
Research Team
1 MD researcher, 4 Post-doctoral fellows, 4 PhD students and 1 Technician
Red blood cells labeled with the membrane potential probe Di-8-ANEPPS.
rBPI21 Mechanism of action Red blood cells imaging by atomic force microscopy (AFM).
IMM REPORT 2009 . 201034 35
IMMUNOLOGY AND INFECTIOUS DISEASES PROGRAMME
02
3534 IMM REPORT 2009 . 2010
RHEUMATOLOGY RESEARCH UNIT
-
TRAP positive monocyte-derived osteoclasts a TNF-antagonist treated
patient (400x magnification).
37
MAJOR INTERESTS
The Unit has two main goals Research in Clinical Nutrition and Metabolism and Education (Undergraduate and post-graduate). Namely the unit promotes an un-dergraduate degree in Dietetics and Nutrition and another on Nutrition and Clini-cal Research education for national and international Medical students, moreover, we sponsor two undergoing Master Programs: Dietetics and Nutrition (in the phase of Projects to attain the Degree) and another in Nutrition, which started in January 2010 in accordance with the implementation of the Bologna process. The Unit also collaborates in International Programs of Advanced Studies: 1) DIETS Network = Dieticians Improving Education Training Standards across Europe, an Erasmus EU financed network Program, in which a close interaction with the UK leading Group is maintained; 2) “RED MEI-CYTED - NETWORK TO EVALUATE DISEASE-ASSOCI-ATED MALNUTRITION” - Network to evaluate disease-associated malnutrition; 3) “COSPI”= Combating Obesity: Strategies for Prevention and Intervention; 4) “DEL-PHI” (EPCRC-project - international scientific EU-funded consortium of Palliative Cancer Care investigators): panel of 17 European, Canadian and US experts on cachexia, aiming to guide treatment, define inclusion criteria for clinical trials and implement results from research into evidence based practice. With regard to Research, the Unit aims to foster clinical, basic and translational Biomedical research, at both national and international level in collaboration with multicentre networks (South and Central America, Spain, Europe). Disease management, nutrition/life-style related risk factors prevail in all lines of investigation. At present, main research areas are focused on: nutrition and cancer, nutrition and Quality of Life, metabolic dysfunction(s), nutrients and disease modu-lation, nutritional therapy and disease prognosis, obesity, fatty liver and insulin resistance. To develop translational research is also a most important goal, hence the progressive focus on: a) genetic polymorphisms of inflammatory mediators or b) humoral regulators, signalling and their receptors, either as pathogenic mecha-nisms of lesion and/or nutritional deterioration or to investigate their implications in metabolism. We also aim to explore changes of cellular nuclei and DNA degra-dation, pathogenic gene mutations in cancer and inflammatory diseases and their interaction with nutrients. Furthermore, investigators deliver services to the Hospital and/or to the Community.
SELECTED PUBLICATIONS
Guerreiro, C.S., Cravo, M.L., Brito, M., Vidal,
P.M., Fidalgo, P.O. and Leitão, C.N. (2007) The
D1822V APC polymorphism interacts with fat,
calcium, and fiber intakes in modulating the risk
of colorectal cancer in Portuguese persons. Am. J.
Clin. Nutr. 85: 1592-1597 (Journal IF: NA)
Machado, M., Marques-Vidal, P. and Cortez-
Pinto, H. (2006) Hepatic histology in obese
patients undergoing bariatric surgery. J. Hepatol.
45: 600-606 (Journal IF: 7.056)
Ravasco, P., Monteiro-Grillo, I., Marques-Vidal,
P. and Camilo, M.E. (2005) Dietary counsel-
ling improves patient’ outcomes: a prospective
randomized controlled trial in colorectal cancer
patients undergoing radiotherapy. J. Clin. Oncol.
23: 1431-1438 (Journal IF: 17.157)
Ravasco, P., Monteiro-Grillo, I., Marques-Vidal,
P. and Camilo, M.E. (2005) Impact of nutrition
on outcome: A prospective randomized controlled
trial in patients with head and neck cancer under-
going radiotherapy. Head & Neck 27: 659-668
(Journal IF: 2.603)
Ribeiro, P., Cortez-Pinto, H., Solá, S., Castro, R.,
Ramalho, R., Baptista, A., Moura, M., Camilo,
M.E. and Rodrigues, C. (2004) Hepatocyte
apoptosis, expression of death receptors and
activation of NF-κB in the liver of non-alcoholic
and alcoholic steatohepatitis patients. Am. J. Gas-
troenterol. 99: 1708-17017 (Journal IF: 6.444)
-
NUTRITION AND METABOLISM UNIT-
Group Leader
MARIA CAMILO
MD, PhD, retired
Other Principal Investigators
Isabel Monteiro Grillo; Helena Cortez-Pinto; Paula Ravasco
Research Team
1 PhD student, 1 MD, 3 Nutritionists and 1 PharmD researcher
he study of the immune response has had major contributions to human health, es-
pecially in what concerns the rational design of vaccination strategies against mul-
tiple infectious agents. The ultimate success case has been the erradication of smallpox
in 1979. However, immunologists and microbiologists still face major challenges, such as
the prevalence of Malaria, Tuberculosis and AIDS, and the emergence of novel strains of
antibiotic-resistant bacteria. Moreover, the incidence of diseases like cancer, allergy and
autoimmune disorders has risen very dramatically in the last half century, posing growing
challenges to immunologists. The Programme of Immunology and Infectious Diseases at
IMM aims at elucidating the basic biological mechanisms that underlie the dynamic inter-
action between the host and distinct pathogens, such as retroviruses (HIV), gamma herpes
viruses, Streptococcus, and parasites - Trypanosome and Plasmodium (Malaria causing
agent). We are also concerned with other aspects of the immune system, particularly its
overactivation in autoimmune diseases (such as rheumatoid arthritis) and allergy. We aim
at testing new protocols that tune down undesirable immune responses. Finally, we inves-
tigate the potent anti-tumor activity of some cells of the immune system (such as T lym-
phocytes), aiming at improving the prospects of treating cancer through immunotherapy.
Altogether, our research is directed at understanding how humans interact with and defend
themselves from pathogens, while maintaining a controlled and healthy immune system.
Only a comprehensive knowledge of these processes will allow the development of novel
and sustainable strategies to combat these major threats to human health.
T
02IMMUNOLOGY AND INFECTIOUS DISEASES PROGRAMME
36 IMM REPORT 2009 . 2010
IMM REPORT 2009 . 201038
02 Immunology and Infectious Diseases Programme
39
MAJOR INTERESTS
The Rheumatology Research Unit is a partnership between IMM and the Rheuma-tology and Bone Metabolic Diseases Department of the Hospital de Santa Maria. Basic scientists and clinicians work side by side with the vision of foster transla-tional research and clinical excellence in the field of Rheumatology. Our specific research aims are the study of the pathogenesis of inflammatory joint diseases (Rheumatoid Arthritis (RA), Juvenile Idiopathic Arthritis, Spondyloar-thropaties and Systemic Lupus Erythematosus (SLE)) and bone disorders (such as osteoporosis) in order to characterize potential tools for early diagnosis and prog-nosis and potential targets for novel therapies.Ongoing research projects are devoted to the study of neutrophils, monocytes and B cells in very early arthritis, to the analysis of the effects of inflammation on atherogenesis in RA and SLE and to the relevance of TNF polymorphisms in the prognosis and pharmacogenetics of several joint diseases. We are also involved in research of bone quality in cooperation with Instituto Superior Técnico. All these research efforts are based on a dual approach, which includes both human samples and animal models of rheumatic diseases. Finally, we are involved in clini-cal trials, development of registries (in collaboration with the Portuguese Society of Rheumatology) and epidemiological studies, in providing services to the commu-nity, including expertise advisory to governmental institutions, and in postgradu-ate teaching.
SELECTED PUBLICATIONS
Ligeiro, D., Fonseca, J.E., Abade, O., Abreu,
I., Cruz, M., Nero, P., Cavaleiro, J., Teles, J.,
Trindade, H., Caetano, J.M. and Branco, J.
(2007) Influence of human leucocyte antigen-
DRB1 on the susceptibility to rheumatoid
arthritis and on the production of anti-cyclic
citrullinated peptide antibodies in a Portuguese
population. Ann. Rheum. Dis. 66: 246-248
(Journal IF: 7.188)
Fonseca, J.E., Cavaleiro, J., Teles, J., Sousa, E.,
Andreozzi, V.L., Antunes, M., Amaral-Turkman,
M.A., Canhão, H., Mourão, A.F., Lopes, J.,
Caetano-Lopes, J., Weinmann, P., Sobral, M.,
Nero, P., Saavedra, M.J., Malcata, A., Cruz,
M., Melo, R., Braña, A., Miranda, L., Patto,
J.V., Barcelos, A., da Silva, J.C., Santos, L.M.,
Figueiredo, G., Rodrigues, M., Jesus, H., Quin-
tal, A., Carvalho, T., da Silva, J.A., Branco, J.
and Queiroz, M.V. (2007) Contribution for new
genetic markers of rheumatoid arthritis activity
and severity: sequencing of the tumor necrosis
factor-alpha gene promoter. Arthritis Res. Ther.
9: R37 (Journal IF: 4.485)
Fonseca, J.E., Cortez-Dias, N., Francisco, A., So-
bral, M., Canhão, H., Resende, C., Castelão, W.,
Macieira, C., Sequeira, G., Saraiva, F., Pereira
da Silva, J.A., Carmo-Fonseca, M. and Viana
Queiroz, M. (2005) Inflammatory cell infiltrate
and RANKL/OPG expression in rheumatoid
synovium– comparison with other inflammatory
arthropaties and correlation with outcome. Clin.
Exp. Rheumatol. 23: 185-192 (Journal IF: 2.364)
Fonseca, J.E., Carvalho, T., Cruz, M., Nero, P.,
Sobral, M., Mourão, A.F., Cavaleiro, J., Abreu,
I., Carmo-Fonseca, M. and Branco, J.C. (2005)
Polymorphism at position –308 of the tumor
necrosis factor alpha gene and rheumatoid ar-
thritis pharmacogenetics. Ann. Rheum. Dis. 64:
793-794 (Journal IF: 7.188)
Fonseca , J.E., Edwards, J.C., Blades, S.
and Goulding, N.J. (2002) Macrophage
subpopulations in rheumatoid synovium: reduced
CD163 expression in CD4+ T lymphocyte-rich
microenvironments. Arthritis Rheum. 46: 1210-
1216 (Journal IF: NA)
-
RHEUMATOLOGY RESEARCH UNIT-
Group Leader
JOÃO EURICO FONSECA
MD (1992) and PhD (2004) in Rheumatology at Faculdade de Medicina da Universidade de Lisboa (FMUL) Auxiliary Professor at FMUL
Other Principal Investigators
Helena Canhão, MD, PhD
Research Team
2 Post-doctoral fellows, 3 MD-PhD students, 5 PhD students, 3 Master students and 2 Trainees
Normal female mice vertebra, with an amplification of 2000 by scanning electron microscopy. The trabecular structure of bone can be clearly observed.
TRAP positive monocyte-derived osteoclasts a TNF-antagonist treated patient (400x magnification).
Transversal paraffin sections from the femoral head of a human patient subjected to hip replacement surgery due to hip osteoporotic fracture by multiphoton microscopy. Collagen detected by the backward-SHG channel (green) is mainly immature collagen fibril segments, indicative of ongoing fibrillogenesis, while collagen detected by the forward-SHG channel (blue) is essentially mature polymerized collagen.
MAJOR INTERESTS
To escape the grip of the human immune system, Trypanosoma brucei, the para-site that causes African sleeping sickness, performs an infamous disappearing act. Every time the host’s immune system gets close to eliminating the infection, a small number of trypanosomes avoids detection by shedding their surface ‘coat’ and putting on a new one, distinct from those the immune system already recognizes. The coat covers the entire surface of the parasite and consists of a dense layer of a Variant Surface Glycoprotein (VSG). This process of changing coats is known as antigenic variation.There are hundreds of VSG genes throughout the trypanosome’s genome, but, at any given time, only one is actively transcribed from a specialized subtelomeric lo-cus known as the expression site. During antigenic variation, some parasites switch transcription to a new expression site, causing the surface coats to change their molecular identity. Such transcriptional changes do not involve alterations in the DNA sequence but are inherited nevertheless, which indicates that VSG transcrip-tion is under epigenetic control. Recently, it was shown that enzymes that remodel or modify the structure of chromatin at expression sites are important for anti-genic variation. Moreover we now know that the active expression site is organized in a more open chromatin structure than of silent sites.Our goal is to understand the role chromatin plays in antigenic variation in T. bru-cei. Specifically, we intend to identify the mechanisms and molecules that define open and closed chromatin structures at expression sites. To that end, we will use modern molecular and genetic strategies including RNA interference, chromatin immunoprecipitation, high-throughput deep sequencing and bioinformatics. Un-derstanding how antigenic variation works should allow us to develop drugs that will disrupt VSG regulation, which in turn, would make parasites, at long last, more vulnerable to the host immune defences.
SELECTED PUBLICATIONS
Figueiredo, L.M. and Cross, G.A.M. (2010)
Nucleosomes are depleted at the VSG expression
site transcribed by RNA polymerase I in African
trypanosomes. Eukaryot. Cell 9: 148-154 (Jour-
nal IF: 3.830)
Figueiredo, L.M., Cross, G.A.M. and Janzen,
C.J. (2009) Epigenetics in African trypano-
somes. Nat. Rev. Microbiol. 7: 504-513 (Journal
IF:14.31)
Yang, X., Figueiredo, L.M., Espinal, A., Okubo,
E. and Li, B. (2009) RAP1 Is essential for
silencing telomeric variant surface glycoprotein
genes in Trypanosoma brucei. Cell 137: 99-109
(Journal IF: 31.253)
Figueiredo, L.M., Janzen, C.J. and Cross, G.A.
(2008) A Histone methyltransferase modulates
antigenic variation in African Trypanosomes.
PLoS Biology 6: e161 (Journal IF: 12.683)
Figueiredo, L.M., Freitas-Junior, L.H. and
Scherf, A. (2002) A central role for Plasmodium
falciparum subtelomeric regions in spatial
positioning and telomere length regulation.
EMBO J. 21: 815-824 (Journal IF: 8.295)
-
PARASITE MOLECULAR GENETICS UNIT-
Group Leader
LUÍSA FIGUEIREDO
PhD (2002) from University of Porto and Institut Pasteur, FrancePost-doctoral research at The Rockefeller University, New York, USAResearch Associate at The Rockefeller University, New York, USA (2008-2009)Group Leader at IMM since 2009
Research Team
to be established
IMM REPORT 2009 . 201040
02 Immunology and Infectious Diseases Programme
41
MAJOR INTERESTS
The Cellular Immunology Unit studies the mechanisms underlying induction and maintenance of immune tolerance. In other words: we attempt to reprogramme the immune system when it is causing disease, as it happens in allergy, autoimmunity and transplant rejection.In the last 50 years, it has been observed a marked increase in the frequency of diseases with immune system disregulation as a direct cause. For instance allergic diseases, which have increased over 200% in the last two decades, or autoimmune diseases, where the immune system attacks normal constituents of the body. In ad-dition, organ transplantation is increasingly more frequent, while rejection medi-ated by the immune system remains the most serious problem for patients. Hence, the control of pathologic immune responses in such situations has been one of the major goals of immunology.Recently, it has become apparent the therapeutic potential of antibodies - mole-cules produced by the immune system capable of identifying with great precision and binding to target components of cells and tissues. One can exploit the antibody specificity in order to block key molecules on the surface of T cells (critical cells in the control of immune responses), thereby using a component of the immune sys-tem to alter immune system function. We research new methods to induce tolerance in allergic asthma, food allergy, and an animal model of rheumatoid arthritis using antibodies or cells from the immune system able to regulate excessive immune re-sponses. Due to differences between animal and human physiology, we collaborate with clinical scientists to validate our experimental data with human samples. We believe that in the foreseeable future antibody therapy, as well as other strategies to modulate the immune system, will be important for improving the quality of life of people suffering from allergy, autoimmunity and transplant rejection.
SELECTED PUBLICATIONS
Oliveira, V., Agua-Doce, A., Duarte, J., Soares,
M.P. and Graca, L. (2006) Regulatory T cell
maintenance of dominant tolerance: Induction of
tissue self-defense? Transpl. Immunol. 17: 7-10
(Journal IF: 1.869)
Graça, L., Le Moine, A., Cobbold, S.P. and
Waldmann, H. (2003) Dominant Transplantation
Tolerance. Curr. Opin. Immunol. 15: 499-506
(Journal IF: 10.455)
Graça, L.*, Lin, C-Y.*, Cobbold, S.P. and
Waldmann, H. (2002) Dominant transplantation
tolerance impairs CD8+ T cell function but not
expansion. Nat. Immunol. 3: 1208-1213 * Joint
first authors (Journal IF: 25.113)
Graça, L., Thompson, S., Lin, C-Y., Adams, E.,
Cobbold, S.P. and Waldmann H. (2002) Both
CD4+CD25+ and CD4+CD25- regulatory cells
mediate dominant transplantation tolerance. J.
Immunol. 168: 5558-5565 (Journal IF: 6.000)
Graça, L., Cobbold, S.P. and Waldmann, H.
(2002) Identification of regulatory T cells in
tolerated allografts. J. Exp. Med. 195: 1641-1646
(Journal IF: 15.504)
-
CELLULAR IMMUNOLOGY UNIT-
Group Leader
LUIS GRAÇA
MD (1995) at Faculdade de Medicina da Universidade de Lisboa (FMUL)PhD (2002) in Immunology at the University of Oxford, UK Post-doctoral research at University of Oxford, UK, and at University of Western Australia, PerthAssistant Professor at FMUL
Research Team
2 Post-doctoral fellows, 4 PhD students and 1 Master student
Flow cytometry data: in vitro expansion of T cells that suppress excessive inflammatory responses (upper right quadrants). (image: V. Oliveira)
Histological section of an asthmatic lung. (image: A. Agua-Doce)
MAJOR INTERESTS
Malaria parasites (Plasmodium) cause severe disease in humans. The parasites de-velop and multiply asexually as haploid forms in red blood cells in the human host and move through a mosquito vector in order to infect new individuals. Transmis-sion from the human host (or a mammalian model such as the mouse) to the mos-quito, and vice versa, represent not only a severe bottleneck in the parasites’ life cycle but depends on the development of specialized cell types with defined pro-teome profiles. Male and female forms differentiate in the blood and once they ar-rive in the mosquito midgut - having been taken up during a blood meal of a female mosquito - participate in fertilization. The resulting diploid zygote develops into a motile ookinete that leaves the midgut pushing through the epithelium to continue its life cycle as an oocyst; sporozoites finally reside in mosquito salivary glands ready to infect a new individual. We have recently shown that post-transcriptional gene silencing is essential for the development of the zygote; 100s of mRNAs are stockpiled in female gametocytes only to be translated after fertilisation. Simi-larly, related mechanisms play a role in the sporozoite. Our aim is to understand the protein factors that are involved in the storage of mRNA and how transcrip-tion, RNA steady state levels and protein synthesis are controlled at these crucial moments. Strategies that interfere with sexual development and thus the Plasmo-dium life cycle may help reduce the spread of malaria.
SELECTED PUBLICATIONS
Mair, G.R., Lasonder, E., Garver, L.S., Franke-
Fayard, B., Carret, C.K., Wiegant, C.A.G.,
Dirks, R.W., Dimopoulos, G., Janse, C.J. and
Waters, A.P (2010) Universal features of post-
transcriptional gene regulation are critical for
Plasmodium zygote development. PLoS Pathog.
6: e1000767 (Journal IF: 9.125)
Baum, J., Papenfuss, A.T., Mair, G.R., Janse,
C.J., Waters, A.P., Cowman, A.F., Crabb, B.S.
and de Konig-Ward, T. (2009) Molecular genetics
and comparative genomics suggests RNAi is not
functional in malaria parasites. Nucleic Acid Res.
37: 3788-3798 (Journal IF: 6.968)
Yuda, M., Iwanaga, S., Shigenobu, S., Mair, G.R.,
Janse, C.J., Waters, A.P., Kato, T. and Kaneko, I.
(2009) Identification of a transcription factor in
the mosquito-invasive stage of malaria parasites.
Mol. Microbiol. 71: 1402-1414 (Journal IF:
5.462)
Mair, G.R., Braks, J.A., Garver, L.S., Wi-
egant, J.C., Hall, N., Dirks, R.W., Khan, S.M.,
Dimopoulos, G., Janse, C.J., and Waters, A.P.
(2006) Regulation of sexual development of
Plasmodium by translational repression. Science
313: 667-669 (Journal IF: 26.372)
Khan, S.M., Franke-Fayard, B., Mair, G.R.,
Lasonder, E., Janse, C.J., Mann, M. and Waters,
A.P. (2005) Proteome analysis of separated male
and female gametocytes reveals novel sex-specific
Plasmodium biology. Cell 121: 675-687 (Journal
IF 29.887)
-
MOLECULAR PARASITOLOGY UNIT-
Group Leader
GUNNAR RUDOLF MAIR
PhD (1998) in Molecular Parasitology at Queen’s University Belfast, UKPost-doctoral research at Queen’s University Belfast, UK, the Leiden University Medical Center, The Netherlands, and Yale Medical School, USAGroup leader at IMM since 2008
Research Team
1 Post-doctoral fellow, 1 Master student, 1 Research student and 1 Technician
Plasmodium sexual development Gametocyte P-bodies
IMM REPORT 2009 . 201042
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MAJOR INTERESTS
Our main objective is to understand the dynamics of populations of bacterial path-ogens and how they respond to selective forces. Current work focuses on char-acterizing the effect of antimicrobial use and human vaccination on the bacterial population. We also investigate the relationships between commensal and disease causing populations of the same bacterial pathogen with the aim of identifying particularly successful clones at causing disease as well as successful colonizers. Current work if focusing on genomic approaches to characterize the differences between these two populations that may explain the associations bacteria estab-lish with the human host.A strong emphasis on quantitative biology has led to development of research in the area of computational biology. We are developing new methods for the quan-titative analysis of partition congruence, comparative genomic analysis and for inferring the phylogenetic relationships between isolates in a bacterial population. These methodologies have been implemented in online tools or freely distributed software.The development of novel laboratory methodologies for the diagnosis of infec-tious diseases is also an active area of research. Investigations followed the recent realization that flow cytometric principles allow the detection of malaria pigment. This finding was explored with the development of this application to the diagno-sis of malaria and it may eventually also be adapted to novel sensitivity tests and evaluated for its usefulness as marker of disease severity.
SELECTED PUBLICATIONS
Hänscheid, T., Egan, T.J. and Grobusch, M.P.
(2007) Haemozoin: from melatonin pigment
to drug target, diagnostic tool, and immune
modulator. Lancet Infect. Dis. 7: 675-685
(Journal IF: 13.165)
Martins, E.R., Pessanha, M.A., Ramirez, M.,
Melo-Cristino, J. and the Portuguese Group
for the Study of Streptococcal Infections (2007)
Analysis of Group B streptococcal isolates from
infants and pregnant women in Portugal revealing
two lineages with enhanced invasiveness. J. Clin.
Microbiol. 45: 3224-3229 (Journal IF: 3.945)
Carriço, J.A., Silva-Costa, C., Melo-Cristino,
J., Pinto, F.R., de Lencastre, H., Almeida,
J.S. and Ramirez, M. (2006) Illustration of a
common framework for relating multiple typing
methods by application to macrolide- resistant
Streptococcus pyogenes. J. Clin. Microbiol. 44:
2524-2532 (Journal IF: 3.945)
Silva-Costa, C., Ramirez, M. and J. Melo-
Cristino (2005) Rapid inversion of the
prevalences of macrolide resistance phenotypes
paralleled by a diversification of T and emm
types among Streptococcus pyogenes in Portugal.
Antimicrob. Agents Chemother. 49: 2109-2111
(Journal IF: 4.716)
Serrano, I., Melo-Cristino, J., Carriço, J.A. and
M. Ramirez (2005) Characterization of the
genetic lineages responsible for pneumococcal
invasive disease in Portugal. J. Clin. Microbiol.
43: 1706-1715 (Journal IF: 3.945)
-
MOLECULAR MICROBIOLOGY AND INFECTION UNIT-
Group Leader
MÁRIO RAMIREZ
PhD (1998) in Molecular Biology at Universidade Nova de Lisboa and at The Rockefeller University, USA Post-doctoral research at Instituto de Tecnologia Química e Biológica, OeirasAssociate Professor at the Faculdade de Medicina da Universidade de Lisboa
Other Principal Investigators
José Melo Cristino, Thomas Hänscheid, João Carriço
Research Team
5 PhD students
Pulsed-field gel macrorestriction profile analysis of group C and G streptococci. A) Dendrogram showing UPGMA cluster analysis of the PFGE profiles. B) PFGE profiles of representatives of each clone.
MAJOR INTERESTS
Malaria, caused by Plasmodium species transmitted between Anopheles mosqui-toes and humans, continues to be a major devastating disease and global public health problem. Annually, more than 300-400 million people worldwide develop malaria and nearly 1 million children die. There is no highly effective, deployable malaria vaccine, and the prevalence of drug-resistant malaria is increasing through-out Africa, Asia and South America which has been associated with increasing mor-bidity and mortality.Today’s efforts towards the control of malaria are limited by our current under-standing of the biology of Plasmodium and of the complex relationships between the three components of the malaria triad—the human, the mosquito, and the para-site. Despite work in each of these systems advancing rapidly in the last decade, the malaria science community is now at a turning point where many believe the success of current and future control efforts will depend on making significant ad-vances in our understanding of the biology of malaria. A potential approach to ma-laria control is to target mechanisms crucial for the development of Plasmodium and/or the pathology caused by its infection. This requires detailed knowledge of the complex host cell-Plasmodium interaction. The Malaria Unit is focusing its ef-forts on the study of mechanisms used by Plasmodium-induced host factors im-portant for the establishment and control of a malaria infection and the pathology associated with it. Determining the host proteins that either promote infections or help ward them off would not only shed light on the basic mechanisms of malaria infection but also provide potential new targets for therapy.
SELECTED PUBLICATIONS
Albuquerque, S.S., Carret, C., Grosso, A.R.,
Tarun, A.S., Peng, X., Kappe, S.H., Prudêncio, M.
and Mota, M.M. (2009) Host cell transcriptional
profiling during malaria liver stage infection reveals
a coordinated and sequential set of biological
events. BMC Genomics 10: 270 (Journal IF: 3.926)
Rodrigues, C.D., Hannus, M., Prudêncio, M., Mar-
tin, C., Gonçalves, L.A., Portugal, S., Epiphanio,
S., Akinc, A., Hadwiger, P., Jahn-Hofmann, K.,
Röhl, I., van Gemert, G.J., Franetich, J.F., Luty,
A.J.F., Sauerwein, R., Mazier, D., Koteliansky, V.,
Vornlocher, H.P., Echeverri, C.J. and Mota, M.M.
(2008) Host scavenger receptor SR-BI plays a dual
role in the establishment of malaria parasite liver
infection. Cell Host Microbe 4: 271-282 (Journal
IF: 7.436)
Epiphanio, S., Mikolajczak, S.A., Gonçalves,
L.A., Pamplona, A., Portugal, S., Albuquerque, S.,
Goldberg, M., Rebelo, S., Anderson, D.G., Akinc,
A., Vornlocher, H.P., Kappe, S.H., Soares, M.P. and
Mota, M.M. (2008) Heme oxygenase-1 is an anti-
inflammatory host factor that promotes murine
Plasmodium liver infection. Cell Host Microbe 3:
331-338 (Journal IF: 7.436)
Pamplona, A., Ferreira, A., Balla, J., Jeney, V.,
Balla, G., Epiphanio, S., Chora, A., Rodrigues,
C.D., Gregoire, I.P., Cunha-Rodrigues, M.,
Portugal, S., Soares, M.P. and Mota, M.M. (2007)
Heme oxygenase-1 and carbon monoxide suppress
the pathogenesis of experimental cerebral malaria.
Nat. Med. 13: 703-710 (Journal IF: 27.553)
Prudêncio, M., Rodriguez, A. and Mota, M.M.
(2006) The silent path to thousands of merozoites:
the Plasmodium liver stage. Nat. Rev. Microbiol. 4:
849-856 (Journal IF: 14.310)
Mota, M.M., Pradel, G., Vanderberg, J., Frevert,
U., Hafalla, J.C.R., Nussenzweig, R., Nussenzweig,
V. and Rodriguez, A. (2001) Migration of Plasmo-
dium sporozoites through cells before infection.
Science. 291: 141-144 (Journal IF: 28.103)
-
MALARIA UNIT-
Group Leader
MARIA MANUEL MOTA
PhD (1998) in Molecular Parasitology at University College London, UKPost-doctoral research at New York University Medical Center, USA Principal Investigator at Instituto Gulbenkian Ciência, Oeiras (until 2005) Associate Professor at the Faculdade de Medicina da Universidade de Lisboa European Science Foundation Young Investigator (2004-2009)International Research Scholar, Howard Hughes Medical Institute, USA (since 2005)
Other Principal Investigators
Miguel Prudêncio
Research Team
6 Post-doctoral fellows, 4 PhD students, 1 Lab manager and 1 Technician
These two images depict hepatocytes in a liver sectionParasites are shown in green, hepatocyte nuclei in blue and > hepatocyte membranes in red.(top panel) This image was performed after one hour of injection Plasmodium sporozoites from the salivary glands of infected mosquitoes and shows one parasite establishing inside one of the hepatocytes. (bottom panel) This image was performed 40 h later and shows a replicating Plasmodium parasite leading to thousands new parasites.
IMM REPORT 2009 . 201044
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MAJOR INTERESTS
Our research is dedicated to T lymphocytes, key players of the immune system, ca-pable of destroying cancer and viral-infected cells, on one hand, but also necessary to prevent the establishment of inflammatory and autoimmune diseases, on the other. These diversified functions derive from the existence of specialized popula-tions of T cells, usually identified by the presence of specific proteins on their cell surface, allowing us to target a particular subset for immunotherapy.In our laboratory we are interested in the processes of generation, activation and regulation of T lymphocytes. Using state-of-the-art research tools (cDNA micro-arrays, RNA interference, bioinformatics), we envisage the identification of mol-ecules involved in those processes and their integration in cellular mechanisms.One of our main areas of study is T cell development in the thymus, using the mouse as a model system. Our goal is to elucidate how cells that protect from infections, on one hand, and from autoimmunity, on the other, are generated, and how the criti-cal balance between these two subsets is set up in the thymus.Our other major focus is the anti-tumor activity of human T cells. We try to dissect the signals that T cells need to receive from tumor cells in order to efficiently elimi-nate them, while also trying to understand how tumors may evade immune surveil-lance through the production of T cell inhibitory factors. We aim at providing funda-mental knowledge that can be used for the rational design of novel immunotherapy strategies against cancer.
SELECTED PUBLICATIONS
Ribot, J.C., deBarros, A., Pang, D.J., Neves, J.F.,
Peperzak, V., Girardi, M., Borst, J., Hayday,
A.C., Pennington, D.J. and Silva-Santos, B.
(2009) CD27 is a thymic determinant of the
balance between IFN- γ - and IL-17-producing
γδ T cell subsets. Nature Immunol. 10: 427-436
(Journal IF: 26.218)
Correia, D.V., d´Orey, F., Cardoso, B.A., Lança,
T., Grosso, A.R., deBarros, A., Martins, L.R.,
Barata, J.T. and Silva-Santos B. (2009) Highly ac-
tive microbial phosphoantigen induces rapid yet
sustained MEK/ Erk- and PI-3K/ Akt-mediated
signal transduction in anti-tumor human γδ T
cells. PloS One: e5657 (Journal IF: NA)
Gomes, A.Q., Correia, D.V. and Silva-Santos, B.
(2007) Non-classical MHC proteins as determi-
nants of tumor immunosurveillance. EMBO Rep.
8: 1024-1030 (Journal IF: 7.099)
Pennington, D.J., Silva-Santos, B., Escorcio-Cor-
reia, M., Silberzahn, T. and Hayday, A.C. (2006)
Early events in the thymus affect the balance
of effector and regulatory T cells. Nature 444:
1073-1077 (Journal IF: 31.434)
Silva-Santos, B., Pennington, D.J. and Hayday,
A.C. (2005) Lymphotoxin-mediated regulation of
γδ T cell differentiation by αβ T cell progenitors.
Science 307: 925-928 (Journal IF: 28.103)
-
MOLECULAR IMMUNOLOGY UNIT-
Group Leader
BRUNO SILVA-SANTOS
PhD (2002) in Immunology at University College London, UK Post-doctoral research at King’s College London, UK Assistant Professor at Faculdade de Medicina da Universidade de LisboaAwarded an EMBO Installation Grant in 2007
Research Team
1 Post-doctoral researcher, 1 Post-doctoral fellow, 3 PhD students and 1 Technician
γδ T cells purified from human peripheral blood were analyzed by confocal microscopy. Antibody labeling for the CD27 receptor, with levels of expression increasing from green to red. (Ana de Barros)
Molecular interactions between γδ T cells and tumor cells, on one hand, and regulatory (Treg) cells, on the other. Key molecular players are indicated. (APC, antigen presenting cell).(See Eur J Immunol 2009, issue 40 (1): pages 6 and 61-70).
MAJOR INTERESTS
We seek to define molecular functions of viral proteins and define their contribu-tion to pathogenesis. There are very few laboratory mouse models of virus infec-tion that enable linking defined viral functions to pathogenesis. Murid herpesvirus 4 (MuHV-4) is one such model and serves as the only mouse model system to study the pathogenesis of Kaposi´s sarcoma herpesvirus (KSHV). KSHV has an etiologi-cal role in Kaposi´s sarcoma, the leading AIDS malignancy, and in certain lympho-mas. The availability of an in vivo model provides a system to test the ability of small-molecule inhibitors of specific viral functions to control infection and thus associated tumorigenesis.We use MuHV-4 to study virus-host interactions. MuHV-4 is inoculated intra-na-sally, causes a transient pneumonia followed by persistence of latent infection in memory B cells. Access to memory B cells is preceded by expansion of infection in germinal centre B cells. Our focus is to identify and characterize molecular func-tions that are crucial for induction of proliferation and differentiation of GC B cells. We then generate recombinant viruses with site-specific mutations that we ana-lyze upon infection of wild type or genetically altered mice. Using this strategy we aim to understand how a defined molecular function of a viral protein contributes to pathogenesis.Thus far, we have defined molecular functions and associated viral phenotypes but we still do not understand the physiology of such phenotypes. For example, the MuHV-4 encoded mORF73 protein is selectively expressed in GC B cells during the expansion of latent infection and it is a strong inhibitor of NF-κB. Viruses lacking this inhibitory function do not expand in GC B cells and do not persist. The question is why does NF-κB, as a key transcription factor, need to be switched off in pro-liferating GC B cells? To address these questions we propose a two fold strategy. To further define the molecular function of key viral proteins to include structural studies, and to develop tools to refine our pathogenesis studies such as tagged vi-ruses that enable in vivo tracking.
SELECTED PUBLICATIONS
Orge, L., Oliveira, A., Machado, C., Lima, C.,
Ochoa, C., Silva, J., Carvalho, R., Tavares, P.,
Almeida, P., Ramos, M., Pinto, M.J. and Simas,
J.P. (2010) Putative emergence of classical scrap-
ie in a background of enzootic atypical scrapie.
J. Gen. Virol. In press (Journal IF: 3.092)
Stevenson, P.G., Simas, J.P. and Efstathiou, S.
(2009) Immune control of mammalian gamma-
herpesviruses: lessons from murid herpesvirus-4.
J. Gen. Virol. 90: 2317-2330 (Journal IF: 3.092)
Rodrigues, L., Filipe, J., Seldon, M.P., Fonseca,
L., Anrather, J., Soares, M.P. and Simas, J.P.
(2009) Termination of NF-kappaB activity
through a gammaherpesvirus protein that as-
sembles an EC5S ubiquitin-ligase. EMBO J. 28:
1283-1295 (Journal IF: 8.295)
Marques, S., Alenquer, M., Stevenson, P.G.
and Simas, J.P. (2008) A single CD8+ T cell
epitope sets the long-term latent load of a murid
herpesvirus. PLoS Pathog. 4: e1000177 (Journal
IF: 9.125)
Pires de Miranda, M., Alenquer, M., Marques,
S., Rodrigues, L., Lopes, F., Bustelo, X.R. and
Simas, J.P. (2008) The Gammaherpesvirus
m2 protein manipulates the Fyn/Vav pathway
through a multidocking mechanism of assembly.
PLoS One 3: e1654 (Journal IF: NA)
-
VIRAL PATHOGENESIS UNIT-
Group Leader
PEDRO SIMAS
PhD (1994) in Viral Pathogenesis at the University of Cambridge, UKPost-doctoral research at the University of Cambridge, UKPrincipal Investigator at Instituto Gulbenkian Ciência (until 1999)Associate Professor at Faculdade de Medicina da Universidade de Lisboa
Research Team
5 Post-Doctoral fellows, 2 PhD students, 1 Technician
Intranasal infection of mice with a luciferase expressing MuHV-4. Splenic infection can be observed.
In situ hybridisation for viral RNAs evidences infected germinal centre cells in splenic sections of MuHV-4 infected mice.
In situ visualization of fluorescently tagged MuHV-4 infected cells.
IMM REPORT 2009 . 201046
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MAJOR INTERESTS
The Clinical Immunology Unit (UIC)’s research focuses on human T cell homeostasis and immune regulation with the ultimate goal of identifying new strategies for im-munological reconstitution and targets for immune-based therapies. An important part of UIC’s research effort is centred on HIV/AIDS immunopatho-genesis mainly through the study of HIV-2, a naturally attenuated form of HIV in-fection. We have shown that in HIV-2, as in HIV-1 infection, CD4 depletion is di-rectly linked to immune activation. HIV-2 is closely related to HIV-1, and has been shown to be equally cytopathic in vitro. Moreover, despite plasma viremia remain-ing low or undetectable throughout HIV-2 infection, we and others have shown that proviral DNA levels do not significantly differ from those found in HIV-1 infected individuals, suggesting a similar ability to disseminate and establish a pool of in-fected cells. Nevertheless, HIV-2 establishes a better equilibrium with the host than HIV-1, leading to a much slower rate of disease progression, lower levels of circulating virus and a limited impact in the mortality rate of the majority of HIV-2 infected adults. The UIC has been intensively involved in the investigation of possi-ble contributing factors to this distinct viral-host equilibrium, taking advantage of the high prevalence of HIV-2 in Portugal due to connections to West Africa where HIV-2 infection is endemic.Other major foci of UIC’s research are: the role of IL-7, a major homeostatic cy-tokine, in human naïve T cell homeostasis; and human T cell development in the thymus with particular interest in the development of regulatory T cells, a subset known to have a key role in protecting from autoimmunity and from the immune-mediated pathology associated to persistent infections. The UIC recently became involved in the diagnosis of primary immunodeficiencies (PID) in response to the National Health Service’s request for a facility in advanced immunologic diagnosis and follow-up which runs in parallel with research in this field. The UIC is also involved in advanced diagnosis in Allergology with some applied research in this area, and receives MDs for 5 month periods, in accordance with the requirements for the allergologist laboratory training in Portugal.
SELECTED PUBLICATIONS
Cavaleiro, R., Baptista, A.P., Soares, R.S.,
Tendeiro, R., Foxall, R.B., Gomes, P., Victorino,
R.M. and Sousa, A.E. (2009) Major depletion of
plasmacytoid dendritic cells in HIV-2 infection,
an attenuated form of HIV disease. PLoS Pathog.
5: e1000667 (Journal IF: 9.125)
Azevedo, R.I., Soares, M.V., Barata, J.T., Ten-
deiro, R., Serra-Caetano, A., Victorino, R.M. and
Sousa, A.E. (2009) IL-7 sustains CD31 expression
in human naive CD4+ T cells and preferentially
expands the CD31+ subset in a PI3K-dependent
manner. Blood 113: 2999-3007 (Journal IF:
10.432)
Albuquerque, A.S., Cortesão, C.S., Foxall, R.B.,
Soares, R.S., Victorino, R.M. and Sousa, A.E.
(2007) Rate of increase in circulating IL-7 and
loss of IL-7Rα expression differ in HIV-1 and
HIV-2 infections: two lymphopenic diseases with
similar hyper immune-activation but distinct
outcomes. J. Immunol. 178: 3252-3259 (Journal
IF: 6.000)
Sousa, A.E., Carneiro, J., Meier-Schellersheim,
M., Grossman, Z., and Victorino, R.M. (2002)
CD4 T cell depletion is linked directly to immune
activation in the pathogenesis of HIV-1 and
HIV-2 but only indirectly to the viral load. J. Im-
munol. 169: 3400-3406 (Journal IF: 6.000)
Grossman, Z., Meier-Schellersheim, M., Sousa,
A.E., Victorino, R.M. and Paul W.E. (2002)
CD4+ T-cell depletion in HIV infection: are we
closer to understanding the cause? Nat. Med. 8:
319-321 (Journal IF: 27.553)
-
CLINICAL IMMUNOLOGY UNIT-
Group Leader
ANA ESPADA DE SOUSA
MD (1986) and PhD (2000) in Clinical Immunology at Faculdade de Medicina da Universidade de Lisboa (FMUL) Investigator and Hon. Assistant Professor at FMUL
Other Principal Investigators
Rui MM Victorino, Maria Conceição Santos, Íris Caramalho, João F Lacerda
Research Team
2 Post-doctoral fellows, 5 Lecturers, 8 PhD students and 2 Research fellows
MAJOR INTERESTS
Immunological studies are crucial for public health providing knowledge for vac-cine development and for the treatment of immune disorders, transplantation rejection, infectious diseases and cancer. Understanding the mechanisms underly-ing differentiation of immune cells and the control of immune responses involves analyses ranging from lymphoid organogenesis to lymphocyte differentiation and function.The adaptive immune system has evolved to control and protect the host from infection. Immune protection involves the generation of immunological memory, through which an individual acquires the capacity to respond better to the initial antigen on re-exposure. Immune and neuronal memory are characterized by obvi-ous differences, but also striking parallels. Indeed, mounting evidence indicates that some of the most potent neurotrophic factors may act on or are produced by immune cells. The neuronal growth factor family includes the glial cell-line derived neurotrophic factor (GDNF) ligands, which signal through the RET tyrosine kinase receptor.Our research projects are centred on the cellular and molecular mechanisms gov-erning haematopoiesis in general and on the role of the proto-oncogene Ret in lymphocyte function in particular. By using state of the art research tools, trans-lated on combined genetic, cellular, and molecular approaches, we plan to observe, quantify and manipulate the functions of the proto-oncogene Ret in the immune system, and to shed light into the mechanisms underlying the development of lym-phoid structures.
SELECTED PUBLICATIONS
Veiga-Fernandes, H., Coles, M.C., Foster, K.E.,
Patel, A., Williams, A., Natarajan, D., Barlow,
A., Pachnis, V. and Kioussis, D. (2007) Tyrosine
kinase receptor Ret is a key regulator in Peyer’s
Patch organogenesis. Nature 446: 547-51
(Journal IF: 31.434)
Peixoto, A., Evaristo, C., Munitic, I., Monteiro,
M., Charbit, A., Rocha, B. and Veiga-Fernandes,
H. (2007) CD8 single-cell co-expression reveals
three different effector types present at distinct
phases of the immune response. J. Exp. Med.
204: 1193-205 (Journal IF: 15.504)
Peixoto, A., Monteiro, M., Rocha, B. and Veiga-
Fernandes, H. (2004) Quantification of multiple
gene expression in individual cells. Genome Res.
14: 1938-1947 (Journal IF: 10.176)
Veiga-Fernandes, H. and Rocha, B. (2004) High
expression of active CDK6 in the cytoplasm of
CD8 memory cells favors rapid division. Nat.
Immunol. 5: 31-37 (Journal IF: 25.113)
Veiga-Fernandes, H., Walter, U., Bourgeois, C.,
McLean, A. and Rocha, B. (2000) Response
of naive and memory CD8+ T cells to antigen
stimulation in vivo. Nat. Immunol. 1: 47-53
(Journal IF: 25.113)
-
IMMUNOBIOLOGY UNIT-
Group Leader
HENRIQUE VEIGA-FERNANDES
PhD (2002) in Molecular and Cellular Biology at Université René Descartes Paris V, FrancePost-doctoral research at NIMR, UK and at the Institut Necker, FranceSenior investigator scientist at NIMR, UK (2006-08) Group leader at IMM since 2008Awarded a European Research Council Starting Grant in 2008Awarded a EMBO Installation Grant in 2008
Research Team
1 Post-doctoral fellow, 1 PhD student, 2 Master students, 2 Technicians and 1 Trainee
Immune cells from mice expressing fluorescence proteins in specific cell lineages. Left: developing lymph node; haematopoietic cells (green-GFP), CD4 (red). Middle: developing lymphoid structure in the intestine; haematopoietic cells (green-GFP), neuronal axons (red). Right: Peyer’s patch; B cell areas (Green-GFP), T cell areas (Red-DsRed).
IMM REPORT 2009 . 201048 49
MAJOR INTERESTS
The hallmark of pathogenic mycobacteria is their ability to survive within the mac-rophage phagosome. Our unit aims to explain this trait by addressing two central questions: First, how do mycobacteria survive within the phagosome hostile en-viroment? And second, how are macrophages able to, under some conditions, kill mycobacteria? For example 90 percent of infected people show no signs of dis-ease. Killing of mycobacteria by macrophages is known to improve by treatment of cells with gamma-interferon, with pro-inflammatory lipids or with extra-cellular ATP - although the mechanism is unknown, all three treatments enhance the matu-ration of pathogenic mycobacterial phagosomes. Our goal is to provide a molecular evidence that proteins in the lumen of the phagosomes directly kill mycobacteria and give the first global map of the proteins in a mycobacterial phagosome, both under normal conditions and under conditions in which killing is enhanced. Finally, we would like to understand the role of pH and redox of the intra-phagosomal envi-ronment and how it contributes to killing.
SELECTED PUBLICATIONS
Kuehnel, M., Rybin, V., Anand, P., Anes, E.
and Griffiths, G. (2009) Lipids regulate P2X7
receptor-dependent actin assembly by phago-
somes via ADP translocation and ATP synthesis
in the phagosome lumen. J. Cell Sci. 122: 499-
504 (Journal IF: 6.4247)
Gutierrez, M.G.*, Mishra, B.B.*, Jordão, M.L.,
Elliot, E., Anes, E. and Griffiths, G. (2008)
NF-kappa B activation controls phago-lysosome
fusion-mediated killing of mycobacteria by mac-
rophages. J. Immunol. 18: 2651-2663 *Joint first
authors (Journal IF: 6.000)
Jordao, L., Bleck, C.K.E., Mayorga, L., Griffiths,
G. and Anes, E. (2008) On the killing of myco-
bacteria by macrophages. Cell Microbiol. 10:
529-548 (Journal IF: 5.598)
Anes, E., Peyron, P., Staali, L., Jordão, L., Gutier-
rez, M.G., Kress, H., Hagedorn, M., Maridon-
neau-Parini, I., Skinner, M.A., Wildeman, A.G.,
Kalamidas, S.A., Kuehnel, M. and Griffiths,
G. (2006) Dynamic life and death interactions
between Mycobacterium smegmatis and J774
macrophages. Cell Microbiol. 8: 939-960 (Jour-
nal IF: 5.598)
Anes, E., Kuhnel, M.P., Bos, E., Moniz-Pereira, J.,
Habermann, A. and Griffiths, G. (2003) Selected
lipids activate phagosome actin assembly and
maturation resulting in killing of pathogenic my-
cobacteria. Nat. Cell. Biol. 5: 793-802 (Journal
IF: 17.774)
-
MYCOBACTERIA-HOST INTERACTIONS UNIT-
Group Leader
ELSA ANES
PharmD (1988) and PhD (1998) from Faculdade de Farmácia da Universidade de Lisboa (FFUL)Visiting Post-doc at EMBL (2000-2005)Associate Professor at FFUL
Research Team
1 Post-doctoral fellow, 3 PhD students and 3 Master students
MAJOR INTERESTS
We study retroviruses molecular interactions with the host cell and their replica-tion strategies, aiming to apply these knowledge for biotechnological applications. We try to understand the mechanisms underlying the function of cellular restric-tion factors and co-factors in HIV-1 and HIV-2 infection. Specifically, we focus on the viral protein Vif and investigate how it can protect viral infectivity of HIV by neutralizing APOBEC family proteins. We are interested in answering questions related to the mechanism of APOBEC3G inhibition and regulation and how Vif can overcome the antiviral restriction of this family of deaminases. This goal is inte-grated in a broader study to comprehend the host-virus interaction at the cellular and molecular level. Here, the importance of cellular proteins and miRNA to help or restrict HIV-1 infection is crucial to develop concepts of viral infectivity or latency. By using shRNA libraries and zinc-finger libraries we look into elucidate the major pathways of virus interaction with the cell.In a different angle of research and using our understanding of HIV biology we aim to identify biochemical targets for viral neutralization and develop molecular strat-egies to block HIV-1 infection. We are interested in impede viral entry into cells and intervene in viral latency. The strategies we explore are based on the platform of synthetic zinc-fingers and engineering antibody-derived protein scaffolds that use unique variable regions. Our goal is to develop more effective and adapted strate-gies that can explore the weaknesses of the virus. Ultimately our unit wants to explore HIV-1 replication strategies for biotechnology purposes, generate novel therapeutic proteins and better gene therapy strategies by programming cell targeting of engineered lentiviral particles.
SELECTED PUBLICATIONS
Aires da Silva, F., Corte-Real, S. and Goncalves,
J. (2008) Recombinant antibodies as therapeutic
agents: pathways for modeling new biodrugs.
BioDrugs 22: 301-314 (Journal IF: 2.253)
Corte-Real, S., Fonseca, L. , Barbas, C.F. 3rd,
Chang, Y., Moore, P. and Goncalves, J. (2008)
Intrabody-based mapping of Latency-Associated
Nuclear Antigen from Kaposi’s Sarcoma-Asso-
ciated Herpesvirus show conserved epitopes for
viral latency inhibition. Virology: Res. Treatment
1: 43-48 (Journal IF: NA)
Ferreira, G.N., Encarnação, .J.M., Rosa, L.,
Rodrigues, R., Breyner, R., Barrento, S., Pedro,
L., Aires da Silva, F. and Goncalves J. (2007)
Recombinant single-chain variable fragment and
single domain antibody piezoimmunosensors for
detection of HIV1 virion infectivity factor. Bio-
sens Bioelectron. 23: 384-392 (Journal IF: 5.243)
Santa-Marta, M., Aires da Silva, F., Fonseca,
A.M., Rato, S. and Gonçalves J. (2007) HIV-1
Vif protein blocks the cytidine deaminase activ-
ity of B-cell specific AID in E. coli by a similar
mechanism of action. Mol. Immunol. 44: 583-
590 (Journal IF: 3.555)
Corte-Real, S., Collins, C., Aires da Silva, F.,
Simas, .J.P., Barbas, C.F. 3rd, Chang, Y., Moore,
P. and Goncalves, J. (2005) Intrabodies targeting
the Kaposi sarcoma-associated herpesvirus
latency antigen inhibit viral persistence in
lymphoma cells. Blood 106: 3797-3802 (Journal
IF: 10.432)
-
RETROVIRUSES AND ANTIVIRAL RESEARCH UNIT-
Group Leader
JOÃO GONÇALVES
PhD (1996) at EMBL, Heidelberg, GermanyResearch Assistant at Harvard Medical School, USAPost-doctoral research at Scripps Research Institute, USAAssociate Professor at Faculdade de Farmácia da Universidade de LisboaAwardee of the Human Frontier Science Program
Other Principal Investigator
Inês Soeiro
Research Team
1 Lecturer, 3 Post-doctoral fellows, 6 PhD students and 3 Research assistants
Synthetic zinc-finger binding to DNA.
HIV infectivity and neutralization by antibodies.
Model of VH-derived small domain antibody.
iNOs/ M.tb Cathepsin Z/ M.tbActin+AA / M.tbActin/ M.tb
J774 macrophages infected with M. tuberculosis. Killing effectors by phagolysosome fusion: Nitric oxide mediated by iNos, Arachidonic acid mediated actin nucleation, Cathepsin Z acquision and NF-KB translocation to the nucleus.
EXTE
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IMM REPORT 2009 . 201050 51
03NEUROSCIENCES PROGRAMME
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IMM REPORT 2009 . 201052 53
eurological diseases represent important medical and socioeconomic problems
and raise fascinating neuroscience questions. At the IMM we use basic scientif-
ic approaches to study major disorders of the nervous system, such as Alzheimer’s dis-
ease, Parkinson’s disease, Huntington’s disease, or stroke. It is likely that the molecular
profiles of diseased brains and nerve cells, established by genomic, proteomic and other
approaches, will identify a myriad of alterations that may be causal to the diseases or
physiological processes under study. Importantly, we couple novel molecular approaches
with extensive expertise in pharmacology and functional neuroscience. The close prox-
imity to clinical groups also enables us to position ourselves as leaders in translational
research. Our research is directed at advancing the understanding of the nervous system
to the point where rational strategies can be developed to better treat and prevent these
devastating conditions. Throughout the history of neuroscience, the investigation of neu-
rological conditions has catalyzed, often driven, neuroscientific discovery. Therefore, in
many of our studies we also explore what neurological impairments can teach us about
normal neural functions.
N
03NEUROSCIENCES PROGRAMME
MAJOR INTERESTS
The general aim of this Unit is to study the clinical epidemiology of diseases involv-ing the nervous system. The research at the NCRU focuses on large samples and on prevalent, disabling, chronic CNS conditions such as dementia, stroke and Parkin-son’s disease. Our aim is to identify risk factors, including ecological and genetic ones, and prognostic factors leading from an independent life to dependency or death, and on the development and assessment of therapeutical interventions that could be able to delay or prevent transition from a healthy, independent state to dis-ability and death. Measures of disability will include neurophysiological and quality of life dimensions. The large samples of patients afflicted with such chronic condi-tions offer a unique opportunity for detailed neuropsychological and neuroimaging studies and the development and testing of cognitive neuropsychological models.
NCRU has an additional aim of training young medical and non-medical clinical re-searchers and to disseminate an evidence-based and patient-oriented view of the research and practice in Clinical Neurosciences.
SELECTED PUBLICATIONS
Canhão, P., Ferro, J.M., Lindgren, A.G., Bousser,
M.G., Stam, J., Barinagarrementeria, F.; ISCVT
Investigators (2005) Causes and predictors of
death in cerebral venous thrombosis. Stroke 36:
1720-1725 (Journal IF: 6.499)
Costa, J., Borges, M., David, C. and Vaz-Car-
neiro, A. (2006) Efficacy of lipid-lowering drug
therapy for diabetic and non-diabetic patients: a
meta-analysis of randomized, controlled trials.
BMJ 13: 1115-1124 (Journal IF: 12.827)
DeRouen, T.A., Martin, M.D., Leroux, B.G.,
Townes, B.D., Woods, J.S., Leitão, J., Castro-
Caldas, A., Luis, H., Bernardo, M., Rosenbaum,
G. and Martins, I.P. (2006) Neurobehavioral ef-
fects of dental amalgam in children: a randomized
clinical trial. JAMA 295: 1784-1792 (Journal IF: )
Emre, M., Aarsland, D., Alberto, A., Byrne, E.J.,
Deuschl, G., De Deyn, P.P., Durif, F., Kulisevsky,
J., van Laar, T., Lees, A., Poewe, W., Robillard,
A., Rosa, M.M., Wolters, E., Quarg, P., Tekin, S.
and Lane, R. (2004) Rivastigmine for dementia
associated with Parkinson’s disease. N. Engl. J.
Med. 351: 2509-2509 (Journal IF: 50.017)
Ferro, J.M., Canhão, P., Stam, J., Bousser, M.G.,
Barinagarrementeria, F.; ISCVT Investigators.
(2004) Prognosis of cerebral vein and dural sinus
thrombosis: results of the International Study
on Cerebral Vein and Dural Sinus Thrombosis
(ISCVT). Stroke 35: 664-670 (Journal IF: 6.499)
-
NEUROLOGICAL CLINICAL RESEARCH UNIT
Group Leader
JOSÉ M. FERRO
MD (1975) and PhD (1987) at Faculdade de Medicina da Universidade de Lisboa (FMUL)Full Professor and Chairman at FMUL and the Santa Maria Hospital
Other Principal Investigators
Alexandre Castro-Caldas, Alexandre de Mendonça, Isabel Pavão Martins, José Pimentel, Cristina Sampaio, Teresa Paiva, Sofia Oliveira
Research Team
7 Post-doctoral clinical investigators, 2 Post-doctoral fellows, 29 MD researchers, 2 PhD students, 12 Technicians and 3 Trainees
IMM REPORT 2009 . 201054 55
MAJOR INTERESTS
The Neuromuscular Unit is aimed to perform clinical, neurophysiological, neu-ropathological and molecular research in neuromuscular disorders. Our scientific activity covers many areas, including neuropathies, myopathies, myasthenia gravis, motor neuron disease, respiratory function and neuro-rehabilitation. Over the last years we have target our main endeavor in two main diseases: familial amyloid polyneuropathy (FAP) and amyotrophic lateral sclerosis/motor neuron disease (ALS/MND). In FAP we investigated markers of early disease involvement, using neurophysiology and skin biopsy to study epidermal nerve fibers. That approach in essential in testing new compounds to treat these patients. In ALS/MND we are interested in: sensitive respiratory function tests, including neurophysiology; res-piratory rehabilitation; exercise; wireless control of respiratory management; neu-rophysiological studies; measurement disease progression and molecular biomar-kers. Moreover, we investigate brain plasticity, caffeine effect on motor cortex, motor unit recruitment, muscle abnormalities in obesity, mitochondrial changes in myopathies and specific phenotypes of motor neuron disease. We collaborate in several international projects.
SELECTED PUBLICATIONS
de Carvalho, M., Pinto, S. and Swash, M. (2008)
Paraspinal and limb motor neuron involvement
within homologous spinal segments in ALS.
Clin. Neurophysiol. 119: 1607-1613 (Journal
IF: 2.971)
Conceição, I., Castro, J.F., Scotto, M. and de
Carvalho, M. (2008) Neurophysiological mark-
ers in familial amyloid polyneuropathy: early
changes. Clin. Neurophysiol. 119: 1082-1087
(Journal IF: 2.972)
de Carvalho, M., Dengler, R., Eisen, A., England,
J.D., Kaji, R., Kimura, R., Mills, K., Mitsumoto,
H., Nodera, H., Shefner, J. and Swash, M.
(2008) Electrodiagnostic criteria for diagnosis of
ALS: consensus of an International Symposium
sponsored by IFCN. Clin. Neurophysiol. 119:
497-503 (Journal IF: 2.972)
Pinto, S., Turkman, A., Pinto, A., Swash, M.
and de Carvalho, M. (2009) Predictive value of
respiratory tests for respiratory insufficiency in
amyotrophic lateral slerosis. Clin. Neurophysiol.
120: 941-946 (Journal IF: 2.972)
Pugdahl, K., Fuglsang-Frederiksen, A., Johnsen,
B., Tankisi, H., de Carvalho, M., Fawcett,
P., Labarre-Vila, A., Liguori, R., Nix, W. and
Schofield, I. (2010) Variation in the neurophysi-
ological examination of Amyotrophic Lateral
Sclerosis in Europe. Amyotr. Lat. Scler. In press
(Journal IF: 1.815)
-
NEUROMUSCULAR UNIT-
Group Leader
MAMEDE DE CARVALHO
MD (1985) at Faculdade de Ciências Médicas, Universidade Nova de LisboaPhD (2000) at Faculdade de Medicina da Universidade de Lisboa (FMUL)Associate Professor at FMUL
Research Team
4 Lecturers, 4 MD researchers, 3 PhD students, 1 Master student and 1 Speech therapist
Stable motor responses recorded from the diaphragm by phrenic nerve stimulation
Unstable complex motor unit of the diaphragm in a patient with ALS
Cortical silent period represented by a period of inhibited muscle activation after brain magnetic stimulation during muscle contraction
Laboratory room: magnetic stimulation apparatus, on the left, and electromyograph
MAJOR INTERESTS
In a time when the world population is rapidly aging, our existence faces ever grow-ing challenges. The brain, the amazing machine which controls the essence of our existence, is not ‘immune’ to the aging process. Unfortunately, for reasons we do not yet fully understand, many of us end up suffering of the so called ‘neurodegen-erative diseases’, such as Alzheimer’s, Parkinson’s, multiple sclerosis, or the Prion diseases. Alzheimer’s disease, the most common neurodegenerative disorder, af-fects about 3% of people over 65 years old, but the number rises to a scary 50% in people over 85. It is estimated that by 2040, about 80 million people around the world will be affected by Alzheimer’s disease. Likewise, it is estimated that over 6 million people are currently affected by Parkinson’s disease. We use molecular biology to dissect the molecular mechanisms which lead to neu-rodegeneration in diseases such as Parkinson’s, Huntington’s, or Alzheimer’s dis-ease, which are all intimately associated with protein misfolding and aggregation in specific regions of the brain. Because the molecular pathways involved in protein homeostasis are highly con-served, we employ a wide variety of model organisms, from the simple but power-ful budding yeast to mammalian cell culture and mice. We also employ sophisti-cated microscopy techniques, such as confocal and multi-photon microscopy, to unveil the mechanisms underpinning neurodegeneration.Our ultimate goals are to develop novel therapeutic approaches for these and other related disorders. We are working closely together with clinicians in order to accelerate drug discovery efforts, translating basic research into clinical applica-tions that will improve the lives of patients.
SELECTED PUBLICATIONS
Outeiro, T.F. and Ferreira, J. (2009) Current and
future therapeutic strategies for Parkinson’s dis-
ease. Curr. Pharm. Des. 15: 3968-3976 (Journal
IF: 4.399)
Outeiro, T.F., Klucken, J., Bercury, K., Tetzlaff, J.,
Putcha, P., Oliveira, L.M., Quintas, A., McLean,
P.J. and Hyman, B.T. (2009) Dopamine-induced
conformational changes in alpha-synuclein. PLoS
One 4: e6906 (Journal IF: NA)
Outeiro, T.F., Kontopoulos, E., Altmann, S.M.,
Kufareva, I., Strathearn, K.E., Amore, A.M.,
Volk, C.B., Maxwell, M.M., Rochet, J.C.,
McLean, P.J., Young, A.B., Abagyan, R., Feany,
M.B., Hyman, B.T. and Kazantsev, A.G. (2007)
Sirtuin 2 inhibitors rescue alpha-synuclein-me-
diated toxicity in models of Parkinson’s disease.
Science 317: 516-519 (Journal IF: 28.103)
Bodner, R.A., Outeiro, T.F., Altmann, S., Max-
well, M.M., Cho, S.H., Hyman, B.T., McLean,
P.J., Young, A.B., Housman, D.E. and Kazantsev,
A.G. (2006) Pharmacological promotion of
inclusion formation: a therapeutic approach for
Huntington’s and Parkinson’s diseases. Proc.
Natl. Acad. Sci. USA 103: 4246-4251 (Journal
IF: 9.380)
Outeiro, T.F. and Lindquist, S. (2003) Yeast cells
provide insight into alpha-synuclein biology and
pathobiology. Science 302: 1772-1775 (Journal
IF: 28.103)
Willingham, S., Outeiro, T.F., DeVit, M.J.,
Lindquist, S.L. and Muchowski, P.J. (2003)
Yeast genes that enhance the toxicity of a mutant
huntingtin fragment or alpha-synuclein. Science
302: 1769-1772 (Journal IF: 28.103)
-
CELL AND MOLECULAR NEUROSCIENCE UNIT-
Group Leader
TIAGO FLEMING OUTEIRO
PhD (2004) at the Whitehead Institute for Biomedical Research, MIT, EUAPost-doctoral research at Harvard Medical School and at FoldRx Pharmaceuticals, USACo-founder of BioEPI Clinical and Translational Research Center, PortugalGroup leader at IMM since 2007
Other Principal Investigator
Teresa Pais
Research Team
7 Post-doctoral fellows, 5 PhD students, 1 Master student, and 3 Technicians
Primary cortical neurons expressing alpha-synuclein. The N-terminus of the protein is labeled in green and the C-terminal is labeled in red. The merged image is shown in yellow.
03 Neurosciences Programme
IMM REPORT 2009 . 201056 57
MAJOR INTERESTS
Body functions are regulated at least, in part, by reflex mechanisms which are included in the autonomic nervous system which activity despite essentially au-tonomous depends in certain conditions from the voluntary control. Obeying in a broader definition to the concept of reflex arc, the main efferent pathways of this system are the sympathetic and the parasympathetic nervous systems. Being a system that struggles to maintain a dynamic equilibrium of visceral functions, the autonomic nervous system is able to mask its own dysfunction. However, when this becomes impossible due to functional disease, physical damage of the neu-ronal network or during the ageing process, an autonomic failure or dysautonomy is declared. An autonomic failure can be primary or secondary in its origin. In the first case, is due to a failure in the system itself and in the second case is con-comitant to other pathologies like parkinsonisms, diabetes, neurocardiovascular syndromes (eg, syncope, hypertension, atrial fibrillation), sleep disturbances, dis-eases of urinary bladder, sexual organs and gastrointestinal system among others. In clinical medicine, the evaluation of the autonomic nervous system involves a set of standardized procedures which purpose is to define whether autonomic func-tion is normal or not, and in the last case to establish a diagnosis, a therapeutic and to follow-up patients. In physiology, human subjects and animal models are used to deeply understand the mechanisms that are underlying the normal autonomic function and its alterations as well as to develop new ways of autonomic evaluation that can be more targeted, more accurate and more predictive. In our unit, we are particular devoted to the study of cardio-respiratory regulation by central nervous system areas and to the control of ocular circulation both in normal conditions or in particular disease status such as atrial fibrillation, neurogenic hypertension and glaucoma both in human subjects and animal models. Complementing the function-al studies the application of methodologies of signal processing to detect an au-tonomic signature in the recorded physiological signals (eg, blood pressure, ECG, respiratory rate) that could be a precocious marker of disease or have a prognostic value is also one of the goals of our multidisciplinary group.
SELECTED PUBLICATIONS
Ducla-Soares, J.L., Santos-Bento, M., Laranjo,
S., Andrade, A., Ducla-Soares, E., Boto, J.P.,
Silva-Carvalho, L. and Rocha, I. (2007) Wavelet
analysis of autonomic outflow of normal sub-
jects on head-up tilt, cold pressure test, Valsalva
manoeuvre and deep breathing tests. Exp.
Physiol. 92: 677-686 (Journal IF: 2.910)
Ribeiro, M.A., Cabral, H.O. and Costa, P.F.
(2007) Modulatory effect of NO on sodium cur-
rents in a neuroblastoma cel line: aspects of cell
specificity. Neurosci. Res. 58: 361-370 (Journal
IF: 2.473)
Rocha, I., Gonçalves, V., Bettencourt, M.J. and
Silva-Carvalho, L. (2008) Effect of stimulation
of sublobule IX-b of the cerebellar vermis over
cardiac function. Physiol. Res. 57: 701-707
(Journal IF: 1.653)
Lima, P.A., Vicente, M.I., Alves, F.M., Dionísio,
J.C. and Costa, P.F. (2008) Insulin increases
excitability via a dose-dependent dual inhibition
of voltage-activated K+ currents in differentiated
N1E-115 neuroblastoma cells. Eur. J. Neurosci.
27: 2019–2032 (Journal IF: 3.385)
Delgado, E., Marques-Neves, C., Rocha, I.,
Sales-Luis, J. and Silva-Carvalho, L. (2009) In-
trinsic vasomotricity and adrenergic effects in a
model of isolated rabbit eye. Acta Ophthalmol.
Scand. 87: 443-449 (Journal IF: 2.138)
Oliveira, M., Nogueira-da-Silva, M., Timoteo,
A., Feliciano, J., Sousa, L., Santos, S., Silva-
Carvalho, L. and Ferreira, R. (2009) Inducibility
of atrial fibrillation during electrophysiologic
evaluation is associated with increased disper-
sion of atrial refractoriness. Int. J. Cardiol. 136:
130-135 (Journal IF: 3.121)
-
AUTONOMIC NERVOUS SYSTEM UNIT-
Group Leader
ISABEL ROCHA
PharmD (1991) at Faculdade de Farmácia da Universidade de LisboaPhD (2000) and Agregação (2009) in Physiology at the Faculdade de Medicina da Universidade de Lisboa (FMUL)Associate Professor at FMULBoard member at European Federation of Autonomic Societies (EFAS)Chairman of the EFAS education committee
Other Principal Investigator
Pedro Freire Costa, JL Ducla Soares
Research Team
3 Lecturers, 1 Post-doctoral fellow, 2 MD-PhD students, 2 PhD students and 5 research students
03 Neurosciences Programme
MAJOR INTERESTS
The brain can adjust neuronal activity to its specific needs, by means of substances that either enhance or depress communication between neurones. Our unit focuses on how these substances interplay and are involved in diseases of the nervous sys-tem , using the following approaches: 1) electrophysiology; 2) neurochemistry, 3) imaging and 4) behaviour.Our unit consists of four research groups each headed by a principal investigator. The Neuromodulation and Plasticity Group (Joaquim Alexandre Ribeiro) is devoted to understand the way neurotransmitter release and action is controlled at the synaptic level and its implications in neuronal plasticity and in brain dysfunctions. The Neuroprotection Group (Ana M. Sebastião) investigates mechanisms involved in neuronal protection against common pathological insults such as low oxygen, low glucose, seizures, or in situations that occur in neurodegenerative diseases, such as Alzheimer’s or Parkinson’s disease (lack of neurotrophic factors or peptide dep-osition). The Receptor biology and cognition group (Luisa V. Lopes) is studies how brain structures involved in memory are affected in situations of cognitive decline and their impact on several pathological conditions, namely depression and anxie-ty, epilepsy, neurodegenerative diseases. The Regulation of Neuronal Death group (Maria José Diógenes) works on neuronal cell death mechanisms induced by toxic conditions and the potential role of neurotrophins as neuroprotectors. Of interest is the loss of endogenous neuronal trophic support that occurs in Alzheimer’s dis-ease patients and the group aims to evaluate the potential direct involvement of Aβ aggregates in this loss. Age-related changes of neurotrophins-mediated con-trol of synaptic transmission and plasticity are also a focus of the group.
SELECTED PUBLICATIONS
Sebastião, A.M. and Ribeiro, J.A. (2009) Adeno-
sine receptors and the central nervous system.
Handbook Exp. Pharmacol. 193: 471-534
(Journal IF: NA)
Fontinha, B.M., Delgado-García, J.M.,
Madroñaln, N., Ribeiro, J.A., Sebastião, A.M.
and Gruart, A. (2009) Adenosine A2A receptor
modulation of hippocampal CA3-CA1 synapse
plasticity during associative learning in behaving
mice. Neuropsychopharmacology 34: 1865-1874
(Journal IF: 6.835)
Fernandes, C.C., Pinto-Duarte, A., Ribeiro, J.A.
and Sebastião, A.M. (2008) Postsynaptic action
of brain-derived neurotrophic factor attenuates
alpha7 nicotinic acetylcholine receptor-mediated
responses in hippocampal interneurons. J. Neu-
rosci. 28: 5611-5618 (Journal IF: 7.452)
Chen, J.F., Sonsalla, P.K., Pedata, F., Melani, A.,
Domenici, M.R., Popoli, P., Geiger, J., Lopes,
L.V., de Mendonca A. (2007) Adenosine A2A
receptors and brain injury: broad spectrum of
neuroprotection, multifaceted actions and “fine
tuning” modulation. Prog. Neurobiol. 83: 310-
331 (Journal IF: 9.130)
Queiroz, C., Gomes, C., Pak, A.C., Ribeiro, J.A.,
Goldberg, S.R., Hope, B.T. and Ferre, S. (2006)
Blockade of adenosine A2A receptors prevents
protein phosphorylation in the striatum induced
by cortical stimulation. J. Neurosci. 18: 10808-
10812 (Journal IF: 7.452)
Diógenes, M.J., Fernandes, C.C., Sebastião, A.M.
and Ribeiro, J.A. (2004) Activation of adenosine
A2A receptor facilitates BDNF modulation of
synaptic transmission in hippocampal slices. J.
Neurosci. 24: 2905-2913 (Journal IF: 7.452)
-
NEUROSCIENCES UNIT-
Group Leader
JOAQUIM ALEXANDRE RIBEIRO
MD (1965) at Faculdade de Medicina Universidade de Lisboa (FMUL)PhD (1982) at the University of Edinburgh, UKPost-doctoral Research at the Faculty of Medicine, University of EdinburghHead of the Laboratory of Pharmacology of Instituto Gulbenkian de Ciência until 1997.Full Professor at FMUL
Other Principal Investigators
Ana Maria Sebastião, Luísa Vaqueiro Lopes, Maria José Diógenes
Research Team
2 Post-doctoral fellows, 4 Lectures, 14 PhD students, 8 Master students, 4 Trainees and 5 Technicians
Patch-clamp setup to record bioelectrical signals at synapses to measure ‘on line’ communication between brain neurons
Fluorescence setup to measure intracellular calcium signals in living cells of the brain. Calcium is a mediator of cell communication and also of cell death.
Facility for rodent behavior analysis
IMM REPORT 2009 . 201058 59
FACILITIESAND SERVICESAlongside with research, IMM has a core of resource units, which comple-ment and maintain the high quality scientific environment. Among these are the Management, the Information Systems, Communication & Train-ing and Lab Management. In addition to these transversal units, and in the spirit of shared resources, IMM offers recently equipped state-of-the-art Facilities: Flow Cytometry, Bioimaging, Animal House and Zebra Fish, as well as a Biosafety level 3 Laboratory (P3) and a Histology Service. All Re-source Units have dedicated staff that provides support to IMM commu-nity and develops initiatives to promote research.
5958 IMM REPORT 2009 . 2010
IMM REPORT 2009 . 201060 61
Facilities and Services
MANAGEMENT
Staff
André Fialho (Legal); Claúdia Soeiro (Front desk); Sandra Duarte, Isabel Roque, Inês Bernardes, Vera Rego and Ana Marcelino (Accounts); Andreia Vaz, Maria José Antunes and Filipa Aperta (Project management)
MISSION
The Management Unit is responsible for the overall IMM legal, administra-tive and financial support. At IMM, funding for research projects comes from many different sources – national and international, public and pri-vate – requiring the skills of experienced administrative staff. The unit is responsible for accounts and budget management – performing all the required activities to assure proper IMM legal accounts, updated cost ac-counting per project/activity and budget control – and for Project Manage-ment, which deals with all administrative and financial aspects of research projects, from the moment of funding approval to the date of final report.We also manage Human Resources, undertaking all tasks necessary to hire new staff and terminate contracts (advertising, selection, contracts prepa-ration, all legally required registrations).Moreover the Management Unit is responsible for tax reporting, a consid-erable task, given the specifications of the legal status of IMM combined with the number and variety of activities and collaboration protocols con-tracted. In addition, within our unit we have a Lawyer who provides legal support to researchers, namely intellectual property issues and consor-tium agreements.
Head of Unit
Margarida Pinto GagoDegree in Management (1978) at Instituto Superior de Economia e Gestão da Universi-dade Técnica de Lisboa
MISSION
The Information Systems Unit provides, manages and develops Informa-tion Technology Resources in order to facilitate the work of IMM research-ers and increase their productivity. Moreover, it enforces process compli-ance with international and internal norms and procedures. The Information Systems Unit designs and implements several projects in the areas of 1) Information System Design; 2) Integration of Existing and New Information Systems; 3) Web Site (in collaboration with the Communication and Train-ing Unit); 4) Documentation and Information Workflow; 5) Physical Support (Backups, Storage, Network Reliability, Network Performance, Security); 6) Knowledge Management. Support to IMM researchers includes the pro-vision of a help-desk support service, assured by a help-desk team that is scheduled in order to respond all working days of the year. This help-desk team receives all help requests from the users, acts as their only contact and, if necessary, works in cooperation with the Information Technology Unit of the Faculdade de Medicina da Universidade de Lisboa.
MISSION
The Lab Management Unit is responsible for the maintenance and safety of the laboratories infrastructures and equipments, ensuring high standards of usability and quality for all research units. Moreover, the unit manages chemical products (namely it supplies carbon dioxide, dry ice, liquid nitro-gen); develops standard operating procedures and implements good health and safety practices, according to National Legislation.Within the Lab Management Unit are the Purchasing Office (Supply Center and Internal Storehouse) and the Washing Room. The Purchasing Office deals with all acquisitions (products, services and equipments), including import / export services and provides information on prices, ongoing promotions, new products and delivery conditions. In addition, a stock of consumables is maintained in the Storehouse, for the IMM community. The Washing Room provides researchers with clean and sterilized material, being equipped for all types of materials and solutions, required by IMM researchers.
INFORMATION SYSTEMS
LAB MANAGEMENT
Leading Consultant
Tito Santos Silva
PhD (2003) in Computer Science at Universidade Técnica de LisboaAssociate Professor at Universidade Católica Portuguesa, Lisbon
Lab Manager
Alexandra Maralhas
Scientific Advisor
Luís Moita
Staff Daniel Silva (PhD student),Pedro Eleutério (Helpdesk)Ruben Alves (Systems analyst)
Staff
Rita Soares and Alexandre Jesus (Lab manager assistants); Sandra Lopes and Ana Rita Vicente (Purchasing office); Edna Gomes, Paula Correia and Fernanda Vila-Chã (Washing room technicians)
MISSION
The Communication & Training Unit was created in 2008 to develop a co-ordinated framework for Science Communication, Advanced Training and Science Funding.The Unit has dedicated staff to address Science in Society activities, in collaboration with national and international partners. A science communi-cation programme has been developed, which builds on researchers’ initia-tives as well as projects aiming at developing two-way interaction between scientists and a wide range of audiences. Activities include workshops, hands-on activities for younger generations, meetings involving scientists and different publics, the development of educational resources and com-munication training activities for scientists. Schools and the media are among privileged partners/audiences.At UCOM we are responsible for the overview and coordination of Advanced Training initiatives. Moreover, we provide the link with the “Centro Académi-co de Medicina de Lisboa” and foster collaborations amongst national and international education and/or research institutions. The core of our educa-tional programmes is the IMM PhD Programme, for which we oversee and guarantee the progress of all its activities, namely: course organization; students’ events; promotion of the programme; and record management. We provide assistance and advice on funding and grant management is-sues. IMM researchers can find support in applying for national and inter-national funding, from provision of funding information to application and contract conclusion.In addition to these three core areas, the Unit also serves a range of trans-versal areas including External Relations, Internal Communication, IMM Events, and Support to the Direction.
COMMUNICATION & TRAININGHead of Unit
Marta AgostinhoPhD (2007) in Biomedical Sciences at Faculdade de Medicina da Universidade de LisboaPost Graduate Diploma (2009) in Science and Society at The Open University, UK.Staff scientist at the IMM since 2009Head of unit since 2008
Staff
Margarida Trindade (Science funding coordinator), Inês Crisóstomo (Advanced training coordinator), Joana Costa (Post-doctoral fellow) Cheila Almeida (Communications assistant), Catarina Rebelo (Trainee)
FAC
ILITIES AN
D SERV
ICES
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MISSION
The IMM is building a P3 Facility that will be ready in the first trimester of 2010. The 70 m2 facility includes two tissue culture rooms and one ani-mal room. All rooms will be fully equipped and will meet the highest safety standards as defined by European and International guidelines. Work to be carried out in this Facility includes all experimental procedures involving Mycobacterium tuberculosis, HIV and other Biosafety level 3 pathogens. The IMM’s P3 Facility will follow strict admission rules. Accordingly, the Fa-cility will provide education, training, and guidance for researchers and will be constantly monitored by dedicated staff. The Facility will be available to IMM internal and affiliated researchers, as well as external researchers from academia, pharma and biotech.
P3 FACILITYCoordinator
Miguel PrudêncioPhD (2000) from University of East Anglia (UK)Post-doctoral research Fellow at University of Leiden (The Netherlands) and at the IMMStaff scientist at the IMM since 2008Head of Facility since 2009
MISSION
The BioImaging Unit acts as a support structure to help and nurture re-search done with Light Microscopy inside the institute. Besides managing resources, the unit provides IMM scientists and visitors with excellence in scientific know-how and expertise in using light microscopy methods for their research. We assist in planning microscopy-oriented projects, choos-ing materials and equipment, analyzing experimental results, processing acquired images and presenting data. Together with continuous training of new users, we organize regular courses to introduce users to the most recent microscopy techniques and foster interactions and collaborations between microscopy users at the IMM.The BioImaging Unit presently manages 3 laser scanning confocal micro-scopes, 2 widefield systems and other imaging resources such as brightfield microscopes and a bioluminescent imaging system. The systems are pre-pared for advanced applications which include live cell imaging, automated long-term time-lapse and intra-vital imaging, enabling researchers to image dynamic events and molecular interactions inside living cells and tissues with state-of-the-art techniques such as FRET, FRAP and Photoactivation. Access to top-of-the-line microscopy systems is becoming a widespread necessity for groups with challenging research programs. Such high-cost systems represent a significant investment which may exceed the financial capabilities of a single group. For this reason, we also provide advice and act as a communication and decision making platform for groups interest-ed in the joint acquisition of novel imaging systems.
BIOIMAGINGHead of Unit
José RinoPhD (2007) in Biophysics at Faculdade de Ciências da Universidade de LisboaPost-doctoral research fellow at the IMMStaff scientist at the IMM since 2009Head of Facility since 2008
Staff
António Temudo (Support)
FLOW CYTOMETRY
Staff
Ana Luísa Caetano (Technician) Alexandre Varela (Technician)
MISSION
The Flow Cytometry Unit provides a state of the art flow cytometry service to the IMM and external researchers. It is currently equipped with a Becton Dickinson FACSAria high speed cell sorter, a FACSCanto and two FACSCali-burs cell analysers. It also has separate computers and software to enable researchers to analyse their data. The cell analysers are available 7 days a week-24 hours a day by trained researchers. The FACSAria is operated by dedicated UCF staff at normal Monday to Friday working hours. Our unit also provides education, training, and advice to researchers wishing to use flow cytometry in their studies. It runs regular flow cytometry courses tar-geted at new users that combines lectures on flow cytometry principles and research applications with practical hands-on tutorials. Occasional seminars are also presented by Unit members or invited speakers on new techniques and instrumentation. Our Unit is used by nearly all the research units of the IMM, with an average of 300 hours of flow cytometer usage per month in 2009. UCF staff is also actively involved in research projects in collaboration with other IMM Units.
Head of Unit
Maria SoaresPhD (2002) in Immunology at University College LondonPost-doctoral research fellow at University College London, UK and at IMM.Staff scientist at the IMM since 2008Head of Facility since July 2009
MISSIONThe IMM has currently one conventional animal facility, and is starting a new, larger barrier Facility. The conventional facility is currently used at 100% of its capacity by IMM researchers. All husbandry and manipulation procedures are performed according to high standards of biocontainment and bioexclu-sion, in order to ensure the best possible conditions in terms of health and safety. The new barrier facility, with an area of 800m2, will host mice and rats in microisolators and individually ventilated cages, and will be available for both IMM and external researchers. The new animal quarters include one Production facility, one Experimental Facility, one Quarantine room, two Transitional Animal Rooms, one Surgical Suite, two Procedure Rooms and one gamma irradiation suite. The new quarters include a biosafety level 3 room and one procedure room. The Animal Facility provides both internal and external services for academia, pharma, biotech and other fields of re-search. These services will range from animal hosting to procedure perform-ance such as: experimental procedures, surgical procedures, in vivo imaging and assisted reproduction techniques such as timed pregnancies, in vitro fertilization, embryo & cesarean derivation, and cryopreservation. This unit is highly committed to follow the 3Rs principles – Replace, Reduce and Refine – for a conscious and responsible use of laboratory animals, while supporting high quality science. For this reason, it provides education, training and guidance for researchers, according to Portuguese and inter-national law and recommendations for good practices and animal welfare.
ANIMAL FACILITYHead of Unit
Domingos HenriquePhD (1991) at Universidade de LisboaPost-doctoral research at NIMR and ICRF, UK and at the Institut d’Embryologie Cellulaire et Moléculaire, FranceInvestigator at Faculdade de Medicina da Universidade de Lisboa Head of Facility since 2008
Staff
Lara Alina Costa (Manager), Dolores Bonaparte (Veterinarian), Yuri Leite (Technician), Joel Filipe (Technician), Olena Pinho (Animal caretaker), Nuno Inacio (Animal caretaker), Jose Vila-Cha (Animal caretaker)
MISSION
The IMM Histology Service results from collaboration between IMM and the Histology Institute of Faculdade de Medicina da Universidade de Lis-boa (FMUL), and aims to provide technical work, expertise and know-how in Histology techniques to IMM researchers. The Histology Service is located in the Histology facilities of the FMUL Histology Institute, and is open dur-ing normal Monday to Friday working hours. The Service staff includes a permanent technician, with a degree in Technical Pathology, Anatomy and Histology.The Histology Service main tasks are: processing tissue samples for rou-tine histochemical procedures; training new users in sample preparation; and providing tutorship in the design and implementation of different his-tology techniques.
HISTOLOGY SERVICECoordinator
Sandra CasimiroPhD (2007) in Molecular Biology, at FCUL, Lisbon, PortugalPost-doctoral research fellow at IMM since 2007
MISSION
The zebrafish (Danio rerio) is a representative of the vertebrate species and is used for the study of human genetic diseases such as cancer, car-diovascular disorders, neurological diseases, inflammation, angiogenesis, muscle-associated diseases and osteoporosis. Unlike humans, the ze-brafish is one of the few vertebrate species that can fully restore the shape, structure and function of body parts lost after severe injury or amputation. Therefore, it has become a powerful model for regenerative medicine.The IMM zebrafish facility hosts approximately 3000 fish in a state-of-the-art housing system. Besides wild type lines there are 16 transgenic lines and 22 mutant lines available to the IMM scientific staff for research pur-poses. As a service we can provide technical help to manage line stocks, identify transgenic and mutant lines, electroporate DNA and morpholino oligos into adult fish, microinject DNA, RNA and morpholino oligos into em-bryos, cell/tissue transplantation in embryos. The IMM zebrafish facility is committed to follow the 3Rs principles – Replace, Reduce and Refine – for responsible use of laboratory animals.
ZEBRAFISH FACILITYHead of Unit
Leonor SaúdePhD (2001) in Developmental Biology at University College London, UKPost-doctoral fellow at Instituto Gulbenkian de Ciência (IGC)Group Leader at IGC (2005-07) and at IMM since 2008Assistant Professor at Faculdade de Medicina da Universidade de LisboaHead of Facility since 2008
Staff
Lara Carvalho (Manager), Fábio Valério (Technician), Patrícia Matos (Part-time technician)
Staff
Inês Matos (Technician)
Staff
Andreia Pinto (Technician)
Facilities and ServicesFA
CILITIES A
ND
SERVIC
ES
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ABOUT IMM
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TechnoPhage, SA is a Biotech company with labs located at the IMM, under a collaboration protocol signed by these two entities. TechnoPhage, SA is engaged in two main R&D programs:
1) Bacteriophages
R&D of novel products, based on the unique prop-erties of bacteriophages, for the treatment, diag-nosis and prevention of bacterial infections. Cur-rent work is focused on the R&D of new products targeting nosocomial, community and food indus-try infections. Different products are being devel-oped with two main purposes:
a) Specific elimination of antibiotic-resistant bac-teria (therapy and sanitation)
b) Detection and quantification of live microor-ganisms (diagnostic devices)
2) Recombinant antibodies
R&D on recombinant antibodies with therapeutic and diagnostic applications
TechnoPhage, SA continuously strives to increase the number of proprietary products, through an ac-tive R&D program and collaboration agreements. TechnoPhage, SA also delivers R&D services for diverse pharmaceutical companies.
Miguel Garcia Director and Chief Executive Officer
www.technophage.pt
GenoMed is a spin-off company of IMM created in 2004. The mission of GenoMed is to provide cutting-edge technology transfer services rele-vant for medical applications. Currently, GenoMed offers over 100 different tests that are used in diagnosis, prognosis and therapeutic follow up. GenoMed acts in 3 main medical areas:
Oncology: Molecular and Cytogenetics tests for Hemato-Oncology, including follow up of bone marrow transplantation. Molecular diagnosis (FISH) for Oligodendroglio mas and urinary tract cancer. Hereditary breast cancer.
Infectious Diseases: Sequencing the HIV genome to detect resistance to anti-viral therapy. Geno-typing Hepatitis B and C genomes and detecting resistance to anti-viral therapy. Genotyping Hu-man Papillomavirus. Infection by Aspergillus in immuno suppressed patients: detection of Galac-tomannan Antigen.
Genetic Diseases: Alzheimer and Parkinson dis-eases; Fronto-Temporal Dementia. Wilson Dis-ease, Hemochromatosis, Porphyria. Hyperthropic cardiomyopathy; Familial Hypercholesterolemia; Brugada and Long QT Syndromes. Bone Dyspla-sias. Pharmacogenetics: Genetic testing to reduce bleeding associated with Warfarin in take. Genetic testing for management of antidepressive drug therapy. Additionally, GenoMed provides parental and ancestry DNA testing.
Teresa Porta NovaDirector
www.genomed.pt
Technology Transfer
MM aims to be the perfect environment for scientific ideas to grow and turn into products and technologies
that make difference in health care. To achieve this goal IMM develops ties and strategic plans with companies committed to innovation and new solutions for the sake of patients. Partnerships are established to strengthen positions in the competitive markets of drug discovery and diagnostics. These partnerships involve both com-panies incubated by IMM and external companies. IMM is one of the leading founders of the Health Cluster Por-tugal consortium, a group of major players for the devel-opment of health sciences and technologies.
The high quality of work developed by all research units of the IMM, either alone or in collaboration, is generat-ing an increasing level of intellectual property rights (IP
rights), namely a few patents of invention with an acknowledged biomedical and/or pharmaceutical use. At the same time, IMM researchers are becoming more aware of questions related to IP rights or technology transfer.
Therefore, IMM takes on the responsibility and the commitment to support the costs involved in the process of patent application and protection, and shares incoming royalties with the inventors, whether as part of any IP rights licensing process or as the core of any emerging biotechnological start-up.
IMM policy is to encourage researchers to become inventors and help their discoveries to thrive and develop beyond the limits of academy, so that they can contribute for a better quality of life of patients and their families.
TECHNOLOGYTRANSFER
“ (...) IMM develops ties and strategic plans with companies committed to innovation and new solutions for the sake of patients.”
“ IMM policy is to encourage researchers to become inventors and help their discoveries to thrive and develop beyond the limits of academy, so that they can contribute for a better quality of life of patients and their families.”
I
Legend
Innovative approaches to drug discovery include using zebrafish as an in vivo model system in regeneration
TECH
NO
LOG
Y TR
AN
SFER
IMM REPORT 2009 . 201068 69
FROM BENCH TO CLINIC
ecently funded Clinical and Trans-lational Research Grants from
competitive calls:
- 15 ongoing clinical research grants, from the Fundação para a Ciência e Tecnologia. These grants were awarded under the 2007 call for clinical re-search, where IMM had a 48% success rate (National success rate was 19%).
- Maria Mota, a group leader at IMM, won one of the three collaborative research grants under the 2009 call of the Harvard Medical School - Portugal Pro-gramme.
IMM is involved in several research networks from the 7th Framework Programme of the European Commission that aim to search, improve and/or de-velop therapies for neurodegeneration, rheumatoid arthritis and Malaria.
R
IMM fosters clinical and translational research - our mission is to go from bench to bedside, and back to the bench, i.e., not only to take new discoveries and technologies for the clinical practice but also to bring the patient care know-how to the laboratory. The close relationship with hospitals, particularly with Hospital of Santa Maria (HSM), a founding partner of IMM, places the institute in a privileged position to bridge the gap between bench research and clinical practice.
FRO
M B
ENC
H TO
CLIN
ICFrom Bench to Clinic
“ IMM is involved in several research networks from the 7th Framework Programme of the European Commission that aim to search, improve and/or develop therapies for neurodegeneration, rheumatoid arthritis and Malaria.”
Ongoing research projects spam a multiplicity of topics: cancer research, regenerative medicine, car-diovascular diseases, inflammation, neurosciences, infectious diseases and drug discovery. Among these are strictly clinical projects but also projects that arose from the collaboration of basic research and clinical research units.
- IMM Angiogenesis Unit actively collaborates with the radiotherapy department of Hospital de Santa Maria to unveil the biological effects of ionizing ra-diation in the vasculature and cancer development.
- The Cellular Immunology and Rheumatology Re-search Units are actively collaborating, resulting in the recent publication of three papers in internation-al peer-reviewed journals.
- The Cell Biology of the Immune System and Clini-cal Neurological Research Units have a grant to de-cipher the contribution of the immune response for sleep and cardiovascular disorders.
- The Cell and Molecular Neuroscience and Clinical Neurological Research Units work closely together to accelerate drug discovery efforts and develop nov-el therapeutic approaches for Parkinson’s disease.
- The Clinical Neurological Research and the Tissue Morphogenesis and Repair Units are searching for novel risk factors for Genetic diseases, such as Non-syndromic cleft lip, and in parallel developing func-tional studies on an animal model, the Zebrafish.
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IMM REPORT 2009 . 201070 71
From Bench to ClinicFR
OM
BEN
CH
TO C
LINICThe IMM clinical research units – namely, the Neu-
romuscular, the Clinical Neurology Research and the Clinical Oncology Research Units are involved in dif-ferent international multicenter studies such as clin-ical trials and registries. From these collaborations resulted important publications
Costa, L. and Major, P.P. (2009) Effect of bisphosphonates on pain and quality of life in patients with bone metastases. Nat. Clin. Prac. Oncol. 6: 163-174. (Journal IF: 9.113)
CLOTS Trials Collaboration, Dennis, M., Sandercock, P.A., Reid, J., Graham, C., Murray, G., Venables, G., Rudd, A. and Bowler, G. (2009) Effectiveness of thigh-length graduated compression stockings to reduce the risk of deep vein thrombosis after stroke (CLOTS trial 1): a multicentre, ran-domised controlled trial. Lancet 373: 1958-1965 (Journal IF: 28.409)
- Miguel Castanho and his team at the Physical Bio-chemistry Unit succeeded in devising an analgesic dipeptide that was able to cross the Blood-Brain Bar-rier and remain effective. This work was awarded the 2008 Grunenthal Award on Pain and originated the registry of a patent.
- Luís Graça and Marta Monteiro of the Cellular Im-munology Unit won an international entrepreneur-ship contest to develop a business plan for a new cel-lular therapy that reduces the risk of rejection after a liver transplant.
- The 2009 Senior Clinical Research and Career De-velopment of the Harvard Medical School – Portugal Program was awarded to Helena Canhão, an IMM researcher and Rheumatologist at Hospital Santa Maria.
- 2008 Bial Award in Clinical Medicine to João Eurico da Fonseca and team (Rheumatology Research unit)
- 2009 Pfizer Award in Clinical Research to Bruno Silva Santos (Molecular Immunology Unit)
Another area is diagnosis, where the Clinical Immu-nology Unit (Group leader Ana E. Sousa) provides services in the area of Primary Immunodeficiencies in response to the medical community’s needs. Fur-thermore, IMM hosts a spin-off company, GenoMed, which offers over 150 different tests that are used in diagnosis, prognosis and therapeutic follow up.
Besides research and technology transfer to the clinical practice, IMM organizes scientific meetings
and courses, namely the “HIV/AIDS: Closing the Gap between Basic Research and Clinical Practice” (IMM, January 2010). IMM researchers also set-up meet-ings aimed at patients and caregivers such as the “Parkinson’s Disease course for caregivers and fam-ily members” (IMM, May 2010).
To further develop and support clinical biomedical research in Portugal, IMM created a Biobank. This infrastructure reinforces the connections between basic and clinical research, promoting the preven-tion, diagnosis and therapeutics of several diseases.
AWARDS FOR ACCOMPLISHMENTS THAT BRIDGE BASIC AND CLINICAL RESEARCH:
Connolly, S.J., Ezekowitz, M.D., Yusuf, S., Eikelboom, J., Oldgren, J., Parekh, A., Pogue, J., Reilly, P.A., Themeles, E., Varrone, J., Wang, S., Alings, M., Xavier, D., Zhu, J., Diaz, R., Lewis, B.S., Darius, H., Diener, H.C., Joyner, C.D., Wallentin, L.; RE-LY Steering Committee and Investigators (2009) Da-bigatran versus warfarin in patients with atrial fibrillation. N. Engl. J. Med. 361: 1139-1151 (Journal IF: 50.017)
Olanow, C.W., Rascol, O., Hauser, R., Feigin, P.D., Jankovic, J., Lang, A., Langston, W., Melamed, E., Poewe, W., Stocchi, F., Tolosa, E.; ADAGIO Study Investigators (2009) A double-blind, delayed-start trial of rasagiline in Parkinson’s dis-ease. N. Engl. J. Med. 361: 1268-1278 (Journal IF: 50.017)
Sampaio, C. (2009) Can focusing on UPDRS Part II make assessments of Parkinson disease progression more ef-ficient? Nat. Clin. Pract. Neurol. 5: 130-131 (Journal IF: 6.979)
IMM REPORT 2009 . 201072 73
he IMM International PhD Programme in Bio-medical Sciences recruits every year highly
motivated students to join one of the three IMM research programmes: Cell & Developmental Biol-ogy, Immunology & Infectious Diseases and Neuro-sciences.IMM’s mission is to enable these students to be able to carry out independent, original and scientifically significant research, and become critical thinkers. The core of the IMM PhD Programme is the research project, which is complemented by a “student-orient-ed” training component. IMM PhD students are ac-companied by a supervisor and by a Thesis Committee that includes the student´s Tutor. Moreover, students are challenged to organize one or more activities.
The PhD training component has a flexible modular structure that includes:
Nanocourses, IMM offers a wide selection of courses, aimed to provide:fundamental scientific knowledge in specific topics among the IMM research programmes transferable skills training
Workshops, organized by IMM or by other national orinternational research institutionsSeminars and Scientific meetings.
Advanced Training
ADVANCED TRAINING
The IMM is strongly committed to Advanced Training in Biomedicine, in particular through the IMM PhD Programme. IMM is associated to the Faculty of Medicine of the University of Lisbon and with the Santa Maria teaching hospital through the Academic Medical Centre of Lisbon (CAML). CAML is a newly formed consortium aiming to promote the academic dimension in clinical practice, renewing the teaching hospital concept.IMM is also a partner of the Harvard-Medical School – Portugal program and of the Programme for Advanced Medical Education, PFMA.
T
•
•
•
IMM PhD
PROGRAMME
PROGRAMME TEAM
Director
Miguel Carneiro de Moura
Executive Coordinator
Inês Crisóstomo
Programme Manager
Joana Costa
Scientific Advisory Board
António JacintoAna Espada de SousaIsabel Pavão Martins
AD
VAN
CED
TRA
ININ
G
Legend
2009 Students Retreat in Quinta dos Amarelos; Alentejo
IMM REPORT 2009 . 201072
IMM REPORT 2009 . 201074 75
Advanced Course on Protein Expression and Purification: Tips and Tricks
BioImaging Nanocourse on Microscopy
Bioinformatics: What? When? How?
Biology of Cancer
Biostatistics
Cell Adhesion and Migration in Health and Disease
Flow Cytometry Workshop - From Basic Concepts to multicolor analysis
Get the best from your sequences
Interacting with different publics
Nanomedicine
New frontiers, new careers: science outside academia
Presenting your research: from bench to peers
Technology Transfer and Intellectual Property in Biomedicine
Advanced TrainingA
DVA
NC
ED TR
AIN
ING
Students (December 2009)
Students with fellowships from Fundação para a Ciência e Tecnologia (FCT)
Other PhD fellowships:
Axa Research Fund
Marie Curie Initial Training Network
MD Resident (PFMA-FCT)
IMM
PhD Thesis Defenses in 2009
9485
1121
10
Legend
Students, faculty and guests socializing during the 2009 Students Annual Meeting
PhD PROGRAMME IN NUMBERS
:
COURSES OFFERED AT IMM
2009/2010:
IMM REPORT 2009 . 201076 77
Science and Society
A major effort is also placed in regular collaboration with the media, in particu-lar in the promotion of dialogue and opin-ion making in health and biomedicine (e.g. public debate in H1N1 pandemic flu), as well as in highlighting the research devel-oped by IMM teams. A proactive attitude towards the me-dia includes regular production of press releases and articles, support to press conferences and media visits at IMM, contact management and media training for researchers.
Promoting an informed discussion on health and biomedical research issues is a priority for the IMM. This has been achieved through the engagement of researchers and non-scientific partners in the IMM’s Outreach programme. The programme includes training for younger generations, an Annual School Visits scheme (which in 2009 benefited more than 600 students from all levels of schooling), and specific outreach projects, such as the EU Researcher’s Night. In 2009, IMM co-organized the project Setting the Stage in Portugal: researchers and the public come together through theatre, which engaged over 13.000 people.
SCIENCE & SOCIETY
SCIEN
CE &
SOC
IETY
REGULAR COLLABORATION WITH THE MEDIA
:
16
8
1
7
288
13
4
39
27
87
153
237
69
7
81
57
58
64
15
MEDIA TYPE
Specialized
Press
Debate
Public Eng.
Radio
News
Funding
Personnal
General
Internet
Interview
Event
T.V.
News story
Research
Health
Film/Photo
Award
Science
CHANNEL
ARTICLE
TOPIC
4-5/weekParticipations in the media
10-15 / yearPress releases
> 40 /yearMedia visits to IMM
1 / yearTraining course for researchers
1.
3.
Legend
1. School visit to IMM “Exploring the human body”, 4-6 year old children, December 2009
2. ”IMM Restaurant: choose your menu on human cells”. Workshop delivered in a variety of venues (this photo: Fundação Gulbenkian, Lisbon, September 2009)
3. Setting the Stage in Portugal: researchers and the public come together through theatre. Forum theatre and movement theatre performances in Lisbon, September, 2009.
2.
IMM PARTICIPATION IN THE MEDIA (2009)
IMM REPORT 2009 . 201078 79
IMM SEMINARSThese seminars are open to the general scientific community. They happen weekly and the speakers list include group lead-ers from IMM and external experts working in different areas of biomedical research.
CHALK TALKSThese seminars are exclusive to PIs and happen every fort-night. The aim is to discuss current research and internal ad-ministration of IMM.
ePI – SEMINARSInvited external senior researchers give a talk and discuss sci-entific issues with whom interested. All IMM community is in-vited to attend.
PIZZA SEMINARSInformal seminars held biweekly. They are an opportunity for PhD students and postdoctoral fellows to present on-going re-search projects.
THEMATIC SERIESSeminars in specific biomedical topics, organized by research teams from IMM and other research centers from the great Lisbon area. Examples include the Tissue Regeneration forum, Lisbon Area Neurosciences Meetings, Neurosciences Semi-nars, Oncology Series and the Immunology Club.
ANNUAL RETREATSIMM’s Group Leaders and several invited researchers gather every year over a weekend to interact and discuss present and future research at the institute. Similarly, IMM PhD students and post docs organize an annual retreat to discuss science, careers, and life at IMM.
SCIENTIFIC MEETINGS AND WORKSHOPSThe IMM researchers organize regularly national and interna-tional scientific meetings held at IMM, with the support of re-source units. In addition, the Edifício Egas Moniz hosts other similar range of activities, such as workshops, courses, public seminars, special events, to which the IMM staff has free and easy access.
THE IMM SEMINAR SERIES
INCLUDES:
The IMM offers an extensive series of public scien-tific seminars, aimed at providing researchers ac-cess to a wide diversity of scientific topics and cut-ting edge science, technology or clinical practice. Attendance to seminars is greatly encouraged as it is believed to foster scientific discussion of ongoing projects through exchange of ideas, experiences and problem-solving tips.In adition, IMM host initiatives developed by researchers and support staff that aim at creating a team spirit and pleasant environ-ment to work.
The IMM offers an extensive series of public scientific seminars, aimed at providing researchers access to a wide diversity of scientific topics and cutting edge science, technol ogy or clinical practice. Attendance to seminars is greatly encouraged as it is believed to foster scientific discussion of on-going projects through exchange of ideas, experiences and problem-solving tips.
IMM LIFE
IMM Life
Legend
1. After IMM Christmas Party, PhD students deliver the presents offered by IMM collaborators to the children of Pediatrics service, Hospital de Santa Maria, December 2009
2. Stand up commedy scientist and his audience at IMM, July, 2009
3. Eric Wieschaus’ Seminar, January, 2010.
NUMBERS(2009)
IMM scientific meetings and workshops
124
7Scientific Seminars
IMM
LIFE
2. 3.1.
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1 Year in the Life of IMM
1 YEAR IN THE LIFE OF IMM
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05FEBRUARY (AND SEP 10)
Collaboration in prep.VIP visit of a committee lead by Professor André Syro-tard, General Director of INSERM, to establish a scien-tific collaboration between IMM and Inserm, France.
12MARCH
FMUL candidate dayIMM joins Lisbon Faculty of Medi-cine’s open day and reveals its bio-medical research.
20MARCH
IMM Open day on Brain Awareness weekneuroscientists share with secondary school students brain science under the motto The brain and arts.
27APRIL
H1N1 in the public agendaIMM virologist Pedro Simas takes on a long lasting commitment to engage the public in informed discussion on H1N1 pandemic flu, participating in over 80 interventions in the Portu-guese media.
6MAY
João Eurico Fonseca and team (Rheumatology Unit) receive 2008 BIAL Prize in Clinical Medicine from Portuguese President Professor Aníbal Cavaco Silva in a ceremony hosted by the Faculty of Medicine of Lisbon.
16-17MAY
Debating science, careers and IMM2nd PhD students retreat in Alentejo, Portugal.
16MARCH
Renown oncologist Klaus Pantel (Uni-versity of Hamburg, Germany) opens the 2009 edition of the Oncology Series, a seminar series dedicated to clinical investigation in cancer.
5 12 16 20 27 6 16-17 29MAY-SEPFEB MAR APRIL MAY
2009
29MAY
IMM’s new website is launched
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25SEPTEMBER
EU initiative Setting the Stage - Re-searchers and public come together through theatre.
8OCTOBER
Following the protocol signed in De-cember 8, 2008, the official creation of Medical Academic Centre of Lis-bon - a consortium of IMM, Lisbon Medical School and Hospital de Santa Maria - is published in Diário da Re-publica.
30OCTOBER
Innovative cellular therapy developed at IMM by researchers Luis Graça, Marta Monteiro and David Cristina win 2nd prize at the international compe-tition Idea To product®, in Texas, USA. The innova-tive therapy prevents complications after liver transplantation.
24NOVEMBER
Award Ceremony where Bruno Silva Santos and team (Molecular Immunology Unit) received Pfizer Prize in Clinical Medicine.
29SEPTEMBER
External Evaluators from the Institu-tional Evaluation Programme of the University of Lisbon visit the IMM.
12-13OCTOBER
3rd PhD Students MeetingUnder the students initiative, IMM re-ceives Nobel Prize winning Tim Hunt and discusses biomedical science.
2010MAY-SEP
On Monday and Wednesdays’ evenings, the seminar room was con-verted in theatre rehearsal room for scientists/actors to develop research-related plays.
Several areas of medicine will be discussed at the campus of the Academic Medical Centre of Lisbon. The Meeting “HIV/AIDS: Closing the gap between basic research and clinical practice” (IMM, January) was the first of such clinical research events.The course on Parkinsons’ Disease for caregivers and family members (IMM, April) brought together researchers, clinicians and caregivers. Young IMM researchers have already won a national contest of entrepreneurship and may represent Portuguese universities later on in the international competition.Novel ways of engagement with partners and citizens are being developed, by initiating IMM presence at social and professional networks.The visit from the International Scientific Advisory Committee of the IMM Neurosciences Programme is scheduled to occur late in 2010.
1 Year in the Life of IMM
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