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Center for Preventive Doping Research

Institute of Biochemistry

European Monitoring Center for Emerging Doping Agents

Activities of the European Monitoring Center for Emerging

Doping Agents

Mario Thevis

Institute of Biochemistry / Center for Preventive Doping Research , German Sport University Cologne, Germany

& European Monitoring Center for Emerging Doping Agents (EuMoCEDA), Cologne/Bonn, Germany

WADA / Science and Investigations Symposium, October 28th 2014, Istanbul

European Monitoring Center

for

Emerging Doping Agents




collect information

Information

Internet sources

Data bases

Scientific literature

Confis-cated

material

Meetings /

Conferences Patents


for


collect information / identify relevant developments collect information / identify relevant developments / question & summarize





for


other monitoring centers

laboratories

industry

police / customs control

scientific community

Contact

Collaboration

report mandate

Expert groups

e.g. WADA List Expert Group

review / evaluate / initiate actions




Literature


for



laboratories

industry



Contact

Collaboration

report mandate

Expert groups






Databases


for



laboratories

industry



Contact

Collaboration

report mandate

Expert groups






April 8, 2011




April 18, 2011




Conferences


for



laboratories

industry



Contact

Collaboration

report mandate

Expert groups






May 28, 2006




February 2009




May 2011, Test methods




(2 x 20 kDa)

March 2012, FDA Approval




March 2013, FDA Recall




Confiscations


for



laboratories

industry



Contact

Collaboration

report mandate

Expert groups






(home-made)

testosterone gel dispenser

100 160 220 280 340 400

85.0656

[C5H9O]+

257.1660

[C16H21N2O]+

316.2155

[C19H28N2O2]+

400.2740

[C24H36N2O3]+

modified furazabol conjugate




Internet sources


for



laboratories

industry



Contact

Collaboration

report mandate

Expert groups



http://www.southernsarms.com/




Welcome!

We are the leading provider of SARMS S-4 in the United States.

At www.XXXXXXXXX.com we work very closely with our customers

to provide the very best products available on the market.

Our business is built on a philosophy of strong customer focus,

regular communication with a high degree of quality and value.

November 2005




CF3 N

H

O

OH CH3

O NH

O2N

O

CH3

http://www.jamaicaobserver.com/




Patents


for



laboratories

industry



Contact

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report mandate

Expert groups






GW501516 positives confirmed, three out of 4 riders are from the same BCR Pizza Hut team By Shane Stokes @ 3:10 PM Monday, April 15, 2013

UCI confirms Vargas Barrantes, Mudarra Segura, Morales Castillo,

Villalobos Azofeifa, and Miguel Ubeto provisionally suspended

Sergej Lisin arguably first athlete tested positive with GW1516 (2012)




Science and Technology

February 8, 2014




The amended 2014 Prohibited

List (Section S2.), which now

includes Hypoxia-Inducible Factor

(HIF) activators Xenon and Argon,

will be in force as of September 1.





for



laboratories

industry



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Confiscated Enobosarm

- Seized 2012 by German Customs Control

- 1 kg of pure Enobosarm (MK-2866)




130 kDa 100 kDa

70 kDa 55 kDa

40 kDa

35 kDa

25 kDa

150 kDa

15 kDa

10 kDa

SDS-PAGE:




17934 17936 17938 17940 17942 17944 17946 17948 17950 17952

m/z

0

10

20

30

40

50

60

70

80

90

100

Rel

ativ

e A

bu

nd

ance

[%

]

17942.8717941.88

17943.88

17940.88

17944.89

17939.85

17945.88

17938.88

17946.89

17937.86 17947.87

17948.9017936.8317949.86

17935.8517952.8617950.88

17934.85 17951.96

Unknown black market sample

Monoisotopic mass of the intact protein: 17931.84 Da




pET-32 Ek/LIC Vektor (Novagen):

• Hypothetical peptide including protein tags and MCS:

→ 183 AS, 19.5 kDa

Black market product = „empty“ vector





for



laboratories

industry



Contact

Collaboration

report mandate

Expert groups






World Anti-Doping Agency

Council of Europe (Advisory Group on Science)

World Association of Anti Doping Scientists

DFG Senate Commission on Food Safety

Network of (European) NADOs




Industry partners




Acknowledgments

• German Federal Ministry of the Interior

• World Anti-Doping Agency

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