institut national de la recherche agronomique a mixed research team between inra, inpt-ensat and...
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![Page 1: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/1.jpg)
Institut National de la Recherche Agronomique
A mixed research team between
INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science
X. Fernandez (director)T. Gidenne (associated director)
‘Tissus Animaux, Nutrition, Digestion, Ecosystèmes et Métabolisme’
(‘TANDEM’)
Animal tissues, nutrition, digestion, ecosystems and metabolism
![Page 2: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/2.jpg)
Biological characteristicsof edible tissues
Controlling factorsunder studies
Genetics
Breeding strategies
Slaughter stress
ProteomicsProtein expression,
biomarkers
MetabolomicsMetabolic profiles
Tissue structure & biochemistry
Collaborations for
gene expression
The control of technological and sensory qualities of products from waterfowlsApplied goals of research
Research Group ‘Promété’ (Head : C. Molette)
Proteomes, Metabolism and Quality
Researches in the Promété group
![Page 3: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/3.jpg)
Our main project
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Liver weight (g)
Fat loss (%)
R²=0.33
![Page 4: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/4.jpg)
Our main project
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1000100010001000
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101520253035
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Liver weight (g)
R²=0.14
CV = 54 %
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Fat loss (%) R²=0.14
CV = 54 %
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CV = 54 %
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R²=0.14
CV = 54 %
Understanding the biological mechanisms underlying the variability in te quality of french « Foie Gras »
![Page 5: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/5.jpg)
Scientific approach
A combined approach to characterize hepatic tissue…
Proteomics
2-D electrophoresisShot gun
4 7
PM (
kDa)
pI
4 74 7
PM (
kDa)
pI
1 23 4
5
67
8 910 11
1213 14
1517
18 19 20
21
2223
2425 26
16
2728
2930
31
1 23 4
5
67
8 910 11
1213 14
1517
18 19 20
21
2223
2425 26
16
2728
2930
31
1 23 4
5
67
8 910 11
1213 14
1517
18 19 20
21
2223
2425 26
16
2728
2930
31
1 23 4
5
67
8 910 11
1213 14
1517
18 19 20
21
2223
2425 26
16
2728
2930
31
1 23 4
5
67
8 910 11
1213 14
1517
18 19 20
21
2223
2425 26
16
2728
2930
31
1 23 4
5
67
8 910 11
1213 14
1517
18 19 20
21
2223
2425 26
16
2728
2930
31
1 23 4
5
67
8 910 11
1213 14
1517
18 19 20
21
2223
2425 26
16
2728
2930
31
Structural characterization
Structural histologyElectron microscopy
Metabolic profiling
Triglycerides profilesMetabolomics
![Page 6: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/6.jpg)
Structure colorationfrom « cryo-fixated » samples
An exemple of structural characterization of fatty liver
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High cooking yield Low cooking yield
Ma
trix
rat
e
a a
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a a
b
a a
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20 min post mortem
After chilling
After cooking
Network extraction Computerized calculation of « matrix rate »
![Page 7: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/7.jpg)
20 µm 20 µm 20 µm
20 min post mortem After chilling After cooking
Small and spherical lipid droplets Big and non spherical lipid droplets
Another exemple of structural characterization of fatty liver
Resine inclusions
![Page 8: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/8.jpg)
The use of proteomics for the search of quality biomarkers
Proteins from fatty liver
Small proteins, soluble at low ionic strength
2-D electrophoresis and image analysis
coupled with identification through mass spectrometry
Heavier proteins, non soluble at low
ionic strength
Shot-gun strategy
![Page 9: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/9.jpg)
The use of proteomics for the search of quality biomarkers
pH Gradient : 5-8
Mole
cula
r w
eig
ht
Identification of spots showing differential expression according to
liver quality
Protein/peptides identification using mass spectrometry is currently
running
2-D gel electrophoresis
![Page 10: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/10.jpg)
The use of proteomics for the search of quality biomarkers
Shot Gun
Identification and semiquantification
of proteins and peptides in different
« strips »by mass spectrometry
.
.
.
.
.
Low cooking
yield
High cooking
yield
![Page 11: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/11.jpg)
The use of metabolomics for the search of quality biomarkers
What can happen…
What could apparently happen…
The one who make it happening…
What really happened …
![Page 12: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/12.jpg)
The use of metabolomics for the search of quality biomarkers
Metabolic profiling through NMR spectroscopy
![Page 13: Institut National de la Recherche Agronomique A mixed research team between INRA, INPT-ENSAT and Toulouse Veterinary Faculty of Science X. Fernandez (director)](https://reader035.vdocuments.us/reader035/viewer/2022062802/56649e875503460f94b8b8e5/html5/thumbnails/13.jpg)
Other projetcs
Tissue and cellular targets ?
Differential expression of quality biomarkers according to various pre-
slaughter treatments
Impact of reactivity to aversive stimulations ?
Comparisons of different genotypes obtained from divergent selection on HPA response to
restraint and suspension
Pre-slaughterhandling
Stress responses
Liver quality
Effect of slaughter on product quality