These materials have been developed within the ESF project: Innovation and development of study field Nanomaterials at the Technical University of Liberec

Innovation and Development of Study Field Nanomaterials at the Technical University of Liberec

nano.tul.cz

Our aim is to produce the smallest diamonds

in the world!

NANODIAM = nanodiamonds

in form of layers or powders

They are so small that it's possible to

see them only by Atomic Force

Microscope.

Very thin layer consists of tiny

crystals of diamond, so tiny that one

diamond crystal is of the size of

nanometers, one millionth of a

millimeter 2

Nanodiamond Particles

3

FRAP1

0

50

100

150

200

250

Control Diamond D4 Diamond D5 Diamond M1 Graphite Chromium

mR

NA

copie

sper

1000

HP

RT

1m

RN

Acopie

s

JUN

0

100

200

300

400

500

600

700

800

900

Control Diamond D4 Diamond D5 Diamond M1 Graphite Chromium

mR

NA

copie

sper

1000

HP

RT

1m

RN

Acopie

s

Molecular level Determination of nanodiamonds bioactivity

Gene name Encoded protein PCR product

length

Main inducing stimulus

JUN Jun activation domain binding

protein

118 Cellular stress

FRAP1 FKBP-rapamycin associated

protein 1

141 Genotoxic stress

K.Mitura, Ł.Pułaski, K.Kania, S.Mitura, G.Bartosz: „Biocompatibility assay of nanocarbon preparations based on

transcriptomic stress biomarkers”, Int. Conf. 4-th Nanodiamond and Related Materials jointly with 6-th Diamond and

Related Films, Zakopane, Abstract Book, (2005), 165. 4

Glioblastoma multiforme Glioblastoma multiforme cells were cultured on the

chorioallantoic membrane of chicken embryo and after 7 days of incubation, were treated with carbon nanoparticles administered in ovo to the tumor. Diamond nanoparticles significantly decreased tumor mass and volume and vessel area. Quantitative real-time polymerase chain reaction analysis showed downregulated fibroblast growth factor-2 and vascular endothelial growth factor expression at the messenger ribonucleic acid level. The present results demonstrate antiangiogenic activity of carbon nanoparticles, making them potential factors for anticancer therapy.

M.Grodzik, E.Sawosz, M.Wierzbicki, P.Orłowski, A.Hotowy, T.Niemiec, M.Szmidt, K.Mitura, A.Chwalibog, International Journal of Nanomedicine, 6, (2011), 3041 – 3048.

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Microorganisms &Nanodiamonds

Salmonella E

Listeria m. Staphylococcus a.

6

Salmonella E.

Listeria m. Staphylococcus a.

Nanodiamonds

7

Ag

Salmonella E.

Staphylococcus a.

Listeria m.

8

Au

Salmonella E.

Staphylococcus a. 9

10

In ovo examination

with nanodiamond particles

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6 day of chicken embryogenesis

eye brain heart

12 day of chicken embryogenesis

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E.Sawosz, T.Niemiec, K.Mitura: Chicken

embryon 12 day – vessel system nanodiamond

particle introduced to embryon in the second

day (300ul-50 ppm C ),

NanoSMat, Rome, 2009.

vessel

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CAM of 12-day old chicken embryos implanted with a sponge loaded with; (A) PBS (control),

(B) VEGF (proangiogenic control), (C) diamond nanoparticle suspended in water at a level of 50 ppm

and (D) with aglomerate of nanodiamond placed onto CAM after injection of diamond nanoparticles

(5000 ppm) in ovo

A B

C D

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14-day old chicken embryo’s chorioallantoic membrane (CAM) after

treatment with;

(A,D) control (PBS) and diamond nanoparticles suspended in PBS at a level:

(B,E) 500 ppm, (C,F) 5000 ppm.

A B C

D E F

15

Distribution of diamond nanoparticles and their aglomeration in chicken embryo’s CAM after injection of diamond

nanoparticles hydrosol (5000 ppm) in ovo; (A) diamond nanoparticles flow inside blood vessels, (B) diamond

nanoparticles flow inside blood vessels and aglomerates placed outside the vessels, (C) diamond nanoparticles

aglomerates located arounded vessels to force them, (D) diamond nanoparticles force membrane

A B

C D 16

Nanodiamond Particles and Nanospider

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Centre for Nanomaterials, Advanced Technology & Innovations,

Technical University of Liberec, Czech

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30 kV

Capillary

collector

Electrospinning-apparatus

Centre for Nanomaterials, Advanced Technology & Innovations

Needle electrospinning:

David Lukas

19

E

Water solution of poly(vinyl alcohol)

Centre for Nanomaterials, Advanced Technology & Innovations

Elmarco - Liberec

(WO/2005/024101) A METHOD OF NANOFIBRES PRODUCTION FROM A POLYMER SOLUTION USING ELECTROSTATIC SPINNING AND A DEVICE FOR CARRYING OUT THE METHOD

20 m

Nanofibers

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Filtration Tisuue engineering Drug delivery Membrains Soundproofed m. Sorbents „Sponge” -Hydrogen container -Ions container etc.

X-rays from Nanospace

DPP addition

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γ - aminobutyric acid

and

N-methyl-4-aminobutyric acid

X-rays from Nanospace

DPP addition

colle

cto

r

25

Few grams of nanospider has the same surface area as of the football field….

Ideas:

• Sponge made from nanospider and modificated

ND inclusions as a containar for hydrogen

• Sponge made from nanospider and modificated

ND inclusion as a source of ions (with very

extended surface)

• After information about application of

detonation ND as solid lubricant - additive for

engine oil – we carry out research in this area.

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Centre of Excellence NANODIAM

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CENTRE OF EXCELLENCE NANODIAM

Thank you for your attention

68thIUVSTA WORKSHOP

„Multifunctional Surface Engineering for Advanced Energy Applications"

December 9-13, 2012. City University of Hong Kong

Results of clinical research – patch test with Nanodiamond Cream

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Clinical research with Nanodiamond Cream

Patch tests on the skin of beck

in patient with allergic diseases 1. Irritation reactions (IR)

2. Allergic reactions

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Bioactivity of diamond

The most important is the bioactivity of diamond powder in human organism.

This mechanism is based on the influence of diamond on membrane cell and DNA in nucleus.

Diamond Powder is a potential medicine because in examinations in vitro and in clinical research has antioxidant, antiinflammatory, anticancerogenic and antiallergic prpperties.

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Application of Nanodiamond Cream

Antiallergic medicine in Atopic Contact Dermatits, Contact Allergy, Hypersensitivity on metals on human skin in external application.

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Application of Nanodiamond Cream

Paramedical substance in treatment of bacterial skin diseases (staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae).

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Application of Nanodiamond Cream

Cosmetic – cream with antioxidant property, anti-age, hypoallergic, to sensitive skin, for all types of face skin, anti-wrinkle.

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After Plasma modification – possibility of depositon

effectual substance on nanofibres

PCL befor

modification

PCL after plasma modification and

deposition effectual substance on its

surface

PLASMA MODIFICATION OF NANOFIBRES

PU befor

modification

PU after plasma modification and

deposition effectual substance on its

surface

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H

H

H

H

H

H

H

H

H

+

+

H3O+

H2O

H2O

H2O

OH¯

H2

H2O

CO2

O2

N2

Diamond Layer of water Air

Ristein J., Riedel M., Mantel B. F., Stammler M., Ley L.: „Surface doping: a special

feature of diamond”; Journal of Physics: Condensed Matter; 13(2001) 8979-8987

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