influence of storage conditions on survival of selected bacteria in bottled mineral water
DESCRIPTION
2. First let’s take a look at a bigger picture. Normally when looking at pictures of our Planet, it seems very blue, you could almost say that more appropriate name for it would be Planet Water, rather than Planet Earth. Therefore when you read articles about acidification, water-shortage etc. it is very easy to “shake your shoulders” and think -“With so much water on the planet, it can’t be a problem….” Well, Water is far from an abundant resource! 3. The picture above show a sphere that contain all of our water in comparison to the size of the Earth. The large blue sphere has a diameter of 1,385 kilometres (860 miles), with a volume of about 1,38 thousand million cubic kilometres. The sphere includes all the water in the oceans, seas, ice caps, lakes and rivers as well as groundwater, atmospheric water, and even the water in you, your plants and pets. Then, you realize that less than 2.5% of the total volume is fresh-water (the tiny sphere on the picture), of which 68.9% is glaciers and permanent snow, 30.8% is groundwater including soil moisture, swamp water and permafrost and 0.3% is Lakes and Rivers, it becomes more clear that there might be a problem!4. Someone was smart enough to figure out that packaging this vital resource would be a great profit chance. But what are you paying for? Actually, when you buy water in supermarkets, you are NOT paying for the water you drink, you are actually paying for the package of it. In Serbia most commonly used materials are glass (which is more expensive) and plastic (PET). Bottled mineral water must not be treated before packaging. Current Regulations on water quality in Serbia prescribes that in 250 mL there must not be Pseudomonas aeruginosa, nor any faecal coliforms. Though, it is possible for water samples to contain a larger number of total count bacteria, if it is a result of natural growth of autochthonous bacteria during storage period.5. As sanitary indicators, which are used for estimating the probability of the presence of pathogenic microorganisms in the water, are bacteria from human and animal intestines. Number of these indicators of pollution, allows more accurate assessment of the degree of bacterial contamination of the water.The basic requirements that are set in relation to bacteria indicators of sanitary conditions are:- they must share the environment with pathogenic bacteria and be extracted into the environment in large quantities- they must be evenly distributed and more resistant against pathogens in the outside environment- methods of determining these bacteria are required to be simple, fast, and reliable enough.The aim of this study was to investigate the effects of different storage conditions of bottled natural mineral water, on viability of selected bacteria indicators and native bacteria species during storage of the same batch.6. Workflow can be schematically presented in few steps. First of all, the selection of bottled water had to be made. The one that was picked out had the lowest number of culturable bacteria in initial time. That was in order to facilitate their monitoring during a period of storage at two different temperatures. Next step was a selection of bacterial strains that will be used for the inoculation of bottles. Bacteria were isolated from the water like wild strains, and were identified and confirmed by Vitek 2 Compact System. In order to determine the behavior of selected bacteria recovery tests were conducted.A total of 60 bottles of natural mineral water were inoculated with the following bacteria: Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa and Citrobacter freundii. Half of these bottles were stored at room temperature, and the other half in the fridge temperature. Under the same conditions, 60 non-inoculated bottles from the same batch were stored. At appropriate time intervals, samples were taken from both temperatures and membrane filtration was performedTRANSCRIPT
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INFLUENCE OF STORAGE CONDITIONS ON SURVIVAL OF SELECTED BACTERIA IN BOTTLED MINERAL WATER
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Blue Planet Earth
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Blue Planet Earth
1,386,000,000 cubic km (332,500,000 cubic miles)ALL WATER on our planet: oceans, seas, ice caps, lakes and rivers, groundwater, atmospheric water, even water in you, your plants and pets.
2.5% of total volume is FRESH-WATER (that tiny sphere), of which:68.9% glaciers and permanent snow30.8% groundwater (soil moisture, swamp water permafrost )0.3% Lakes and Rivers (that tiny dot)
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Pack it Sell it
What ARE you paying for?
Current regulations in Serbia: NO coliforms, faecal coliforms, faecal enterococci nor Pseudomonas aeruginosa in 250 mL Total count 10* CFU/mL
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The Aim of Our Study
How does different storage conditions (temperature, light) effect the bacteria present in bottled natural mineral water CONTROL sample
How does the number of pathogens change in artificially contaminated water under the same storage conditions
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Workflow
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Materials and Method
Microorganisms for artificial contamination Citrobacter freundii, Escherichia coli, Enerococcus faecium, Pseudomonas aeruginosa (DensyCheck, pour plate)Control sample total count bacteria in original packages
Media for total count: PCA R2A (American Public Health Association, APHA)Membrane filtration: Citrobacter freundii, Escherichia coli - Chromocult Coliform Agar (CCA) Enerococcus faecium - Chromocult Enterococci Agar (CEA) Pseudomonas aeruginosa - Cetrimid Agar (CA)
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Results
Preliminary testing of different brands of bottled natural mineral water
SampleTotal count (CFU/mL)Pseudomonas aeruginosaColiformsFaecal coliforms22 oC37 oCIndndndndndIIbbndndndndIIIndndndndnd
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Results
Temperature profile in room during storage perioddaystemperature
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Results
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Results
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days ResultsCitrobacter freundii
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Results
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Conclusion
All bottles of natural mineral water was in accordance with microbiological safety given in Regulations on the quality of quality and other requirements for natural mineral water, natural spring water and table water.
It was confirmed that use of low-nutritious media, such as R2A gives us more realistic results, when compared to high nutritious PCA, especially when it comes to presence of saprophytic, autochthonous bacteria from the water.
Pathogenic bacteria tend to die off at room temperature more rapidly than at refrigerator temperature, with the exception of P. aeruginosa, which even tend to multiply rapidly at room temperature.
When stored in refrigerator P. aeruginosa kept its initial number during period of 75 days, while other pathogens tend to decrease in number.
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THANK YOU FOR YOUR ATTENTION
MSc Marina [email protected] Dragoljub CvetkoviDr Aleksandra VelianskiDr Sinia Markov
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*Workflow can be schematically presented in few steps. First of all, the selection of bottled water had to be made. The one that was picked out had the lowest number of culturable bacteria in initial time. That was in order to facilitate their monitoring during a period of storage at two different temperatures.
Next step was a selection of bacterial strains that will be used for the inoculation of bottles. Bacteria were isolated from the water like wild strains, and were identified and confirmed by Vitek 2 Compact System. In order to determine the behavior of selected bacteria recovery tests were conducted.
A total of 60 bottles of natural mineral water were inoculated with the following bacteria: Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa and Citrobacter freundii. Half of these bottles were stored at room temperature, and the other half in the fridge temperature.
Under the same conditions, 60 noninoculated bottles from the same batch were stored. At appropriate time intervals, samples were taken from both temperatures and membrane filtration was performed. Incubation was carried out on selective media for 24 - 48 hours at a temperature of 37 C, according to ISO standard.
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