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In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

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Page 1: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

In this presentation you will:explore the importance and use of light

microscopes

Introduction to the Microscope

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Page 2: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

Introduction

Many things that are known and understood today, could not have been discovered without microscopes.This is especially the case with the life sciences.

Microscopes are used to magnify things that are too small to be seen clearly by the naked eye.

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For example, without the microscope we would never have found out that all living things are made from cells.

Page 3: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

"Microscopes are used to magnify things that are too small to be seen clearly by the naked eye."

Is this statement true or false?

Question 1

Answer True or False.

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Page 4: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

"Microscopes are used to magnify things that are too small to be seen clearly by the naked eye."

Is this statement true or false?

Question 1

Answer True or False.True

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Page 5: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

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Parts of the Light Microscope

1. Eye Piece/ Ocular2. Arm3. Low Power Objective4. Stage Clips5. Coarse Focus

Adjustment6. Fine Focus Adjustment7. Revolving Nose Piece8. High Power Objective9. Stage10. Diaphragm11. Aperture12. Light/ Mirror13. Base

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Function

Arm

Coarse focus adjustment

Stage Clips

Eye piece

Eye piece - magnifies objectsSupport arm - holds the lenses in position over the stage

Revolving nose piece Rotating nose piece - for

changing objective lensesClips - to secure specimen slide to the stage

Coarse focus dial - for focusing and large movements of the stage

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Function

Stage

Base

Diaphragm dial

Fine Focus Adjustment

Light/ mirror

Stage - for supporting specimen slideDiaphragm dial - adjusts the amount of light that shines on to the slide

Fine focus dial - for focusing and small movements of the stage

Base - weighted to keep microscope stableThis directs the light upward onto the slide.

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Low Power Objective – a small lens with low magnifying power.

High-Power objective – A large lens with high magnifying power.

Aperture- hole or an opening through which light travels

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What is the purpose of the diaphragm dial on a light microscope?

Question 2

A) To magnify the specimen

B) To hold the glass slide in place

C) To change the objective lens being used

D) To adjust the amount of light that is directed through the specimen

Page 10: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

What is the purpose of the diaphragm dial on a light microscope?

Question 2

A) To magnify the specimen

B) To hold the glass slide in place

C) To change the objective lens being used

D) To adjust the amount of light that is directed through the specimen

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Page 11: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

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Guidelines for Using the Light MicroscopeMicroscopes are expensive precision instruments and must be handled with care.

Keep the instrument in a box or under a cover when not in use.

Use only the supporting arm of the microscope to lift it. Always carry the microscope with one hand underneath the base and the other hand holding the supporting arm.

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Guidelines for Using the Light Microscope

Keep the lenses clean by wiping them with lens tissue.

Never touch the lenses with your fingers.

Always handle microscope slides by their edges.

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Total Magnification

The total magnification of an image is equal to the magnifying power of the eye piece lens multiplied by the magnifying power of the objective lens.

Eye piece

Objective lens

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Total Magnification

The magnifying power of each lens is usually inscribed on the lens barrel.

For example, if a ×10 objective lens is used, assuming the eye piece magnification is ×10, the total magnification of the image will be (×10) × (×10) = × 100.

Eye piece

Objective lens

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Selecting the Lowest Power Objective Lens

To do this, position the ×4 objective lens so that it lies directly above the hole in the stage.

It is normal practice to look at a specimen using the lowest available magnification first, then to look more closely at the specimen using a higher magnification.

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Placing the Slide on the Stage

Before you place a slide on the stage, use the coarse focus dial to position the stage of the microscope so that there is at least a 2 cm gap between the objective lens and the stage.Place the slide on the stage, so that the specimen is over the middle of the hole on the stage.

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Placing the Slide on the Stage

Make sure that light is shining through the slide, and then secure it in place with the clips.

This will help to prevent the slide from being dislodged when you are viewing it.

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Raising the Stage

Looking at the stage and the slide from the side, use the coarse focus dial to raise the stage so that it is as close to the objective lens as possible without the risk of damaging the slide.

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Getting the Specimen into Focus

Looking through the microscope eye piece, use the coarse focus dial to slowly move the stage downward.

Do this until the specimen comes into focus.

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Getting the Specimen into Focus

Try to keep both eyes open as you do this, as it will help to prevent eye strain and headaches. Never move the stage upward when you are looking through the microscope eye piece. This is because, when using the higher powered objective lenses, you can easily crush the slide and break the lens.

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Minor Focusing Adjustments

The fine focus dial alters the height of the stage more accurately than the coarse focus dial.

You may need to make minor adjustments to the height of the stage using the fine focus dial to get your specimen into sharp focus.

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Adjusting the Diaphragm Dial

The diaphragm dial can be rotated to let different amounts of light through the specimen.

Finding the amount of light that produces the best viewing results for each specimen is often a matter of trial and error.

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What part of the microscope is used to make minor focusing adjustments?

Question 3

A) The diaphragm

B) The coarse focusing dial

C) The fine focusing dial

D) The objective lens

Page 24: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

What part of the microscope is used to make minor focusing adjustments?

Question 3

A) The diaphragm

B) The coarse focusing dial

C) The fine focusing dial

D) The objective lens

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Page 25: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

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Biological Drawings

It is good practice to make biological drawings of what you see using a microscope.

The aim of a biological drawing is to provide a clear, labeled account of what you have seen.

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Biological Drawings

Biological drawings are useful because they: allow you to

study a specimen in detail provide a clear, labeled account of what you have seen act as a memory aid to help you remember what you have seen

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Which of the following states why it is useful to make biological drawings from things you see using a microscope?

Question 4

A) To provide a clear, labeled account of what you have seen

B) To help you to remember what you have seenC) To allow you to study the specimen in detailD) All of the above

Page 28: In this presentation you will: explore the importance and use of light microscopes Introduction to the Microscope Next >

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Which of the following states why it is useful to make biological drawings from things you see using a microscope?

Question 4

A) To provide a clear, labeled account of what you have seen

B) To help you to remember what you have seenC) To allow you to study the specimen in detailD) All of the above

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Summary

In this presentation you have seen:

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the purpose of microscopes

how a light microscope should be used

why biological drawings are used