in the name of god. molecular imprinted polymer (mip) supervisor dr. parchehbaf presenter meysam...
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In the name of GOD
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Molecular imprinted polymer(MIP)
Supervisor
Dr. Parchehbaf
Presenter
Meysam abdi
1391/09/152012/12/05
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Content
Introduction
History
Principles
Advantage
Application
Conclusion
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Introduction
Base of the method, is recording of target molecule structure
In Polymer memory at polymerization process.
so that the identification, synthesized polymer only detects the
target molecule.
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History
1894, Fischer lock & key theory.
1930, Polyakov, molecular imprinting in silica’s matrix
1949, Linus pauling theory, change of antibody structures
1977, Gunter wulff, molecular imprinting by using covalence banding
1981, Klaus mosbach, molecular imprinting by using non-covalence banding
1995, Witcombe, molecular imprinting by using semi-covalence (hybrid) banding
www.wikipedia.com www.sciencedirect.com
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Research procedure
C. Alexander, L. Davidson, and W. Hayes, Tetrahedron 59, 2025-2057 (2003).
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Principles
www.biotage.com
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Fischer Lock & Key theory
Monomer & cross linker
Template molecule
bonding
Remove template molecule
PowerPoint of J.jafari thesis
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Variety methods of MIP production
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Advantage
high selectivity
high pre-Concentration factor
the sorbent is reusable for many times
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Application of MIP
MIP
Sensor
Pre-concentratio
n
Pharmacy
Catalyses
Separation
Selective sorption
Purification
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Sensor
Development of a voltammetric sensor based on a molecularly
imprinted polymer(MIP) for caffeine measurement
Step1: A caffeine-selective molecularly imprinted polymer (MIP) and a non-imprinted polymer (NIP) synthesized.
Step2: The MIP, embedded in the carbon paste electrode.
Step3:The prepared electrode was used for caffeine measurement via: 1.analyte extraction in the electrode 2.electrode washing 3.electrochemical measurement of caffeine.
Step4: DPV were used for determination.
A linear range of calibration curve: 6×10-8 to 2.5×10-5 mol/L
The detection limit: 1.5×10-8 mol/L.
T. Alizadeh et al. / Electrochimica Acta 55 (2010) 1568–1574
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Caffeine (1,3,7-trimethylxanthine) is an alkaloid
Caffeine Fountain
Tea
kola nuts
Cacao beans
Coffee
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MIP-CP & NIP-CP responses
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MIP-CP electrode selectivity
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Real sample analysis
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A novel high selective and sensitive para-nitrophenol volta-
-mmetric sensor, based on a MIP-CP electrode
Step1: Para-nitrophenol selective MIP and a non-imprinted polymer (NIP) synthesized
Step2: used for carbon paste (CP) electrode preparation.
Step3: A dynamic linear range of 8×10-9 to 5×10-6 mol /L was obtained. The detection limit of the sensor was calculated as 3×10-9 mol/L.
T. Alizadeh et al. / Talanta 79 (2009) 1197–1203
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Electrode responses & washing effect
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Selection of elecrochemical methods
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Analytical characterization
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Application of an Hg2+ selective imprinted polymer for the preparation of a
novel highly selective and sensitive electrochemical sensor for the
determination of ultra trace Hg2+
Step1: Mercury ion selective cavities were created in the vinyl pyridine based cross-linked polymer.
Step2: The interference of different ionic species with the response of the electrode was also studied.
Step3: This sensor showed a linear response range of 2.5×10-9 to 5.0×10-7 M and lower detection limit of 5.2×10−10 M (S/N).
T. Alizadeh et al. / Analytica Chimica Acta 689 (2011) 52–59
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Hg2+
Properties
Hg2+ can in turn cause symptoms
paranoia
Sleeplessness fever memory
lossweakness
tremors
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Comparison of the IIP-CP & NIP-CP electrodes
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Evaluation the selectivity of sensor
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Determination of Hg2+ in real samples
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Preparation of nano-sized Pb2+ imprinted polymer and its application as the
chemical interface of an electrochemical sensor for toxic lead determination
indifferent real samples
Step1: a new nano-structured ion imprinted polymer (IIP) was synthesized by copolymerization
Step2: A carbon paste electrode modified with IIP-nanoparticles was used for fabrication of a Pb2+ sensitive electrode.
Step3: Differential pulse stripping voltammetry method was applied as the determination technique,
Step4: The introduced sensor showed a linear range of 1.0×10-9 to 8.1×10-7 M and detection limit of 6.0×10-10 (S/N = 3).
T. Alizadeh, S. Amjadi / Journal of Hazardous Materials 190 (2011) 451–459
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Pb2+
importance
toxicological
Effects
interferes with
calciumPoisoner
environmental
pollutant
enzyme inhibitor
Found in Soil
Air
Water
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Comparison of the prepared electrodes
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Evaluation of the selectivity of IIP-CP electrode
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Evaluation of the effect of extraction conditions
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Determination of Pb2+ in real samples by proposed sensor
T. Alizadeh et al. / Talanta 79 (2009) 1197–1203
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Prepared electrode
T. Alizadeh et al. / Talanta 79 (2009) 1197–1203
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a new electrochemical sensor for determination of compounds
at trace levels was introduced. Application of polymer as a
novel modifying agent in the carbon paste electrode made it
very selective for compounds determination in the presence of
common potential interfering agents.
The polymer, used in the carbon paste composition, acted
as the selective chemical interface of the sensor as well as a
pre-concentration agent.
Conclusion