IMPORTANT!!!! PLEASE READ BEFORE USING: CARE AND USE NOTES

E

B

C

D DISTAL

PROXIMAL

FOS (Fiber Optic Stylet) The FOS has been shipped ready for use with the FO-Light. A protective housing has been installed on the distal end of the device to prevent damage during shipping. The housing is inserted into the coupling adapter and can be removed by pulling the housing out of the adapter FIG A. The proximal end also contains a black protective sleeve over the fiber optic connector FIG B. Both of these protective devices should always be used when storing the device. HANDLING The FOS is a glass fiber optic cable and must be handled with care to ensure optimum performance. Though the connector is compatible with other laser light sources, THE FIBER SHOULD ONLY BE USED WITH THE FO-LIGHT SOURCE. The distal end of the fiber has been stripped of its buffer coating. DO NOT BEND THE END OF THE FIBER. A thin protective coating is present to add support, but breakage can occur with excessive bending FIG C. CATHETER INTRODUCTION The FOS has been shipped with a coupling adapter already installed on the distal end FIG D. Once the protective housing has been removed, the coupling adapter is ready for use. Simply insert the bare end of the fiber into the endotracheal tube (typically an IV catheter) FIG E. If you meet resistance, slowly spin the catheter while continuing to push the fiber. The catheter can be secured to the coupling adaptor. When fully inserted, a small section of the fiber will protrude from the end of the catheter. This can be adjusted by loosening the cap on the proximal end of the coupling adapter FIG D allowing the adapter to move along the fiber, and retightening the cap once in the desired location. CLEANING The tip of the fiber may get dirty after continual use. The tip can be cleaned using 70% isopropyl alcohol on a clean lint free tissue. Be careful not to apply pressure to the tip during cleaning as this can result in fiber breakage. STORAGE The FOS should be stored with the protective black sleeve FIG B over the SMA coupler and a rigid support FIG A over the distal end of the fiber to prevent inadvertent breakage.

A

IMPORTANT!!!! PLEASE READ BEFORE USING: CARE AND USE NOTES

FO-LIGHT (Fiber Optic Light Source) The FO-Light has been shipped to you ready for immediate use. A red protective sleeve has been installed over the coupler FIG F and should be retained and used during storage to prevent debris from collecting in the coupler. OPERATION The FO-Light is powered on by rotating the neck of the light source counter-clockwise with the light pointed toward the user FIG F. Rotating the neck clockwise powers off the light source. DO NOT OVER-TIGHTEN the neck in the OFF position. Maximum light intensity is observed at the initial power-on point. Continue to rotate the neck clockwise until the desired intensity is observed. Excessive clockwise rotation will result in removal of the neck from the body of the device. DO NOT ATTEMPT TO SCREW THE NECK BACK ON. Follow the bulb replacement directions in the maintenance section to replace the neck assembly. Improper replacement of the neck onto the body WILL RESULT IN A BROKEN BULB. MAINTENACE The light should not be left on for extended periods of time as it this will decrease battery and bulb life. The batteries should be replaced if there is a noticeable decrease in light output. If light intensity is still diminished, replace the bulb. A replacement bulb is located in the end cap of the device where the batteries are retained. Remove the spring from the end piece and the bulb is stored in a red protective sleeve FIG G. With the batteries removed, unscrew the neck of the device in the ON direction until the neck is completely detached. Remove the old bulb by pulling straight out gently, being careful not to break the bulb (use of paper towel is recommended). The neck assembly can be further disassembled into three pieces, distal piece, optical system, and proximal piece. The following steps are depicted in FIGS H through K. Insert the prongs of the new bulb into the two holes in the top of the plastic bulb housing FIG H. DO NOT INSERT FULLY. Rotating in the OFF direction screw the proximal neck piece back onto the main body by until it is fully seated FIG I. Replace the optical system insert over the bulb. Rotate the proximal neck piece in the ON direction until it meets the insert FIG J. Holding the proximal neck piece in place, screw the distal neck piece onto the proximal neck piece FIG K. Replace the spring in the end piece FIG G and reassemble with batteries installed. THE LIGHT WILL BE ON ONCE END PIECE IS SCREWED INTO PLACE. Power off the FO-Light normally to complete the bulb changing process. STORAGE The FO-Light should be stored in the mesh sleeve that has been included to prevent inadvertent damage from rolling around. The red protective cap should be replaced over the coupler whenever the light is not in use FIG F.

F

G

H

I

J

K

ON OFF

ProximalNeck Piece

Optical System Insert

DistalNeck Piece

IMPORTANT!!!! PLEASE READ BEFORE USING: CARE AND USE NOTES

L

M

N

LIB (Lung Inflation Bulb) This device provides a very Safe and Reliable method for verification of tube placement in place of the use of a dental mirror to verify respiration, which is not reliable because the exhaled air of the sized animals these products are designed for does not consistently result in the typical mirror fogging that is apparent in larger animals, along with other methods of verification where the animal is observed for several seconds with the airway obstructed looking for pronounced abdominal breathing with no visible distention of the chest. HANDLING The Lung Inflation Bulb is constructed from two pieces that are bonded together with a special epoxy. The distal end is fitted with a male luer that will mate with the female luer connector on the trachea tube FIG L. The Proximal end is flexible bulb that when squeezed the there should be obvious distention of the chest followed by an immediate return to normal when released. Incorrect placement will not result in chest distention. Thus the animal is not deprived of oxygen for any period of time and verification is absolute and immediate. STORAGE These devices are designed to be Semi-Disposable meaning they are not designed for rigorous cleaning or sterilization but are capable of Multiple Uses depending on your specific needs. ETT (Endo Tracheal Tube) These are IV catheters FIG M that have a Urethane based catheter in place of the typical PTFE (or Teflon®) catheter. The Urethane catheters exhibit qualities that make them superior to the PTFE catheters in this application. They are much more Softer and more Flexible which minimizes the trauma to the animals who in most cases will be debilitated. HANDLING These will be provided in sterile packaging FIG N but are Not For Human Use and are clearly labeled as such. STORAGE These are disposable and intended as single use devices.

IMPORTANT!!!! PLEASE READ BEFORE USING: CARE AND USE NOTES

RIS (Rodent Intubation Stand) Constructed from durable polycarbonate, this platform provides the perfect stage for intubating mice and rats of all sizes. The stand is constructed from a single piece of polycarbonate plastic with a seamless design. The top of the stand provides a hand rest for stabilization during intubation that is designed to support significant weight. OPERATION The animal is suspended from the incisor wire on the angled side of the stand. Three mounting positions are available to customize the placement of the animal on the stand in order to meet the user's needs. Two sets of posts are provided with each stand for installation of the incisor wire and the optional head positioning wire. Each post has three positions for the height adjustment to optimally accommodate the user and the animal. Each post is also fitted with a slit for securing the end of the wire making the wire easy to install and easier to remove. CLEANING The compact seamless design and its construction from durable polycarbonate make this stand easy to clean and will not crack with the use of harsh cleaning agents as is common with similar stands constructed from acrylic. STORAGE The stand is lightweight and portable so it can be stored in the most convenient location possible. The posts are removable and should be stored with the stand.

O

P

IMPORTANT!!!! PLEASE READ BEFORE USING: CARE AND USE NOTES

Rodent Inhalation Anesthesia Delivery & Scavenging System The system should be installed near electrical supply (outlet), oxygen supply, and exhaust evacuation route to the outside. SETUP The anesthetic vaporizer (not supplied) can be placed in either of two locations, top left side of the box (FIG Q) or over the handle between the two rubber tubes attached. The vaporizer input hose is located nearest the left edge of the box while the vaporizer Output hose is located nearer the center of the box. The exhaust port on the top right of the box should be connected to the external exhaust evacuation with the large evacuation hose. The exhaust hose used should not be longer that 12-15 feet. Make sure all connections of the exhaust hose are completely sealed. OPERATION Activate Scavenging system Verify the 2 inch exhaust hose is securely connected to the exhaust outlet of unit and ceiling port; check all connections for tight fit. Plug the system into a standard 110 volt electrical outlet. The switch located on the top of the unit turns the evacuation blower on/off. Ensure all delivery ports are plugged with rubber stoppers. Activate Induction Box Put mouse/rat in induction box and seal the lid. Make sure white clamp on tubing to induction box is open (close this clamp when induction box is not in use). Close induction box exhaust valve by turning the “T” handle on top of the valve to the off position. Activate Anesthesia System Verify that vaporizer contains isoflurane. Turn on O2 tank. Set flow meter to 2-4 lpm depending on how many stations are in use. Set isoflurane vaporizer to 2.0% (adjust as desired). Observe mouse/rat for anesthetic induction; when mouse/rat has been immobile with steady respiratory rate for 1 minute (approximately 4-5 minutes for first mouse/rat then 2-3 minutes per mouse/rat). Transferring Animals from Induction Box to Workstation Exhaust induction box by turning induction box exhaust valve to "Open" position and close anesthetic gas line to induction box using white tube clamp. Break the seal on the induction box lid and open slightly. DO NOT COMPLETELY OPEN BOX. Wait 5 seconds then open completely, remove mouse/rat, and loosely replace lid. Remove rubber stopper from one of the delivery ports and place mouse/rat's nose into the port. The next animal can be placed into induction box and the exhaust port closed. Shutdown Shut off O2 tank and flow meter. Turn induction box exhaust valve to "Open" position. Switch off the evacuation blower. When induction chamber is not in use but workstations are active, close white tube clamp on anesthetic gas line to the induction chamber. Keep rubber stoppers in all procedure ports when they are not in use. For boxes with two vaporizers, the left vaporizer operates the induction box and procedure ports and the right vaporizer operates the remote anesthetic delivery system.