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IMMUNOLOGY 4315

College of Natural, Applied and Health Sciences/ Dept. of Biological Sciences

Kean University, 1000 Morris Avenue, Union, NJ 07083

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COURSE SYLLABUS

Instructor: Evros Vassiliou, Ph.D.

Dept. of Biological Sciences

Office: Bruce, B120 C

E-mail: evassili@kean.edu

Phone: 1-908-737-3626

Office Hours: For current office hours please use

KeanWise, visit home page

(www.kean.edu/~evassili), or see posting on

office door

Course Number: BIO 4315

Credit Hours: 4 Credit Hours (one, 2 hour 40 min lecture

meeting per week and one, 2 hour 40 min

lab meeting per week)

Course Time & Location: Lectures/Labs:

Tue 4:30-7:15 P.M.

Thu 4:30-7:15 P.M

Lectures in Science Hall C-145

Labs in Science Hall C-154

Prerequisites: 16 credits of Biology including BIO 2400 or

permission of the instructor

Course Description: A fundamental study of the innate and

adaptive immune systems of animals

Suggested Textbook: Immunology, 6

th Edition, W.H. Freeman (Kindt et. al., 2007).

ISBN: 1-4292-0211-4

Required Notebook:

Bound Lab Notebook

Attendance: Attendance is vital for successful course completion. Physician’s

documentation or other official documentation is required to avoid lowering of grade.

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Course Objectives

Upon completion of course students will acquire the following skills

1. Understand basic functions of the immune system

2. Culture specific immune cells

3. Understand the principles of an ELISA and carry out an ELISA for a specific

cytokine

4. Understand the immune system respond to a variety of pathogens

5. Understand basic signaling pathways utilized by TLRs

6. Understand the role of inflammation in pathogenesis

7. Understand the immunomodulatory properties of fatty acids

Academic Integrity Policy

Kean’s academic integrity policy will be strictly enforced. In cases of violations,

appropriate penalties should be expected. Policy is available at University web site.

Letter Grading: � Exams will be multiple choice.

� A total of 3 written exams will be administered. In case of class cancellation,

exam will be given the following period we meet.

� 3 Exams: 100 pts each (300 pts)

� Lab Practicals, Participation, Attendance, Quizzes: 100 pts

Final grade is calculated based on the total number of points accumulated.

Example: Person with 3 exams 90, 95, 98 and 96 pts for lab projects has a total of

379 pts. (379/400)*100 = 95 %. Grade letter is A.

� Grading Scale:

A: 94-100 % B+: 84-89 % C+: 66-71 %

A-: 90-93% B: 78-83 % C: 60-65 %

B-: 72-77 % F:<60

Make-Up Exams:

No make up exams will be given. Final examination will count twice. Official

documentation is required for final examination to count twice.

Laboratory Work

You are expected to record all your activities in your lab notebook in ink. Bring your lab

notebook to every lab class session.

A good lab notebook is one that if you read it 6 months later contains enough information

to allow you to reproduce a specific task.

For technical and logistical reasons no lab make-ups are possible.

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Tentative Lecture And Laboratory Course Outline

Syllabus

Digital Diet

Lab Equipment Introduction (Lab)

Intro to Immune System Chpt. 1

Cells and Organs of Immune System Chpt. 2

Media Preparation (Lab)

Trypsinization (Lab)

Cell Counting (Lab)

Lab Practical Exam I

Test I

Antibodies: Structure and Function Chpt. 4

ELISA Lecture

Stimulation (Lab)

Cytokines Chpt. 12

Toll-like Receptors

TNF-a ELISA Protocol (Lab)

ELISA Reagent List (Lab)

ELISA Report (Lab)

Test II

Magnetic Bead Cell Separation

MHC Chpt. 7

Cell Mediated Immune Responses Chpt. 14

Fatty Acids and the Immune System

Apoptosis Protocol (Lab)

Test III

Flow Cytometry

Immune Responses To Infectious Diseases

Chpt.17

pDC Paper