Image Viewing with WebScope

Page 2

WebScope Overview WebScope

• A platform–independent digital slide viewer. • Does not require client side software installation. • Can be used from any workstation (Macintosh, Linux, etc.). • Uses any Internet browser that supports Adobe Flash Player.

Certain WebScope features are only available when used with an Aperio system in which digital slides are served by Aperio ImageServer. User permission are controlled by the role assigned to the user.

Page 3

WebScope Features WebScope Features Include:

• Create and view slide annotations. • Analyze digital slides. • Adjust brightness and contrast. • Navigate and zoom the digital slide.

Annotation tools

Annotation window

Page 4

WebScope Tools WebScope Main Display

Main toolbar

Thumbnail

Navigation toolbar

Page 5

WebScope Tools Toolbar Summary

Pan

Image Information

WebScope Quick Reference

WebScope Information

Show/Hide Thumbnail

Show/Hide Navigation

Toolbar

Adjust Brightness/Contrast

Show/Hide Annotations

Window

Analyze Digital Slide

Save Annotations

Integrated Color Management

Pen

Negative Pen

Ruler Rectangle

Ellipse

Arrow

Page 6

WebScope Tools Navigation Toolbar Summary

Home: fits entire digital slide to fit

window. Current Magnification

Level

Zoom In/Out

Move Indicated Direction

Magnification Slider bar

Create Link to Digital Slide

Page 7

WebScope Basic Features Open Slide in WebScope

• Depending on the Aperio product, the method of opening a digital slide in WebScope differs.

• Spectrum and Spectrum Education: • Click the thumbnail image to launch WebScope.

If ImageScope is installed, hold down the W key while you click the thumbnail image.

Page 8

WebScope Basic Features Open Slide in WebScope

• KickStart for Educators: • Access the website served by KickStart. • Click WebScope link under the digital slide to be opened.

Page 9

WebScope Digital Slide Navigation Navigation

• Panning - use the mouse to left-click and drag the image (this is the fastest method).

• Use the arrows to move in one direction. • Click anywhere in the thumbnail to move to a new location quickly.

The thumbnail image shows the entire digital slide.

Page 10

WebScope Digital Slide Navigation Change Magnification

• Quickly zoom to max magnification by double-clicking on the image. • Set the magnification to a specific level using the slider icon. • Zoom in and out using the icons. • Rest the view to fit the entire image in the main window using the icon.

Page 11

WebScope Annotations Annotations Window

• Displays details about annotations that have been drawn on the digital slide. • Use the window to:

• Show or hide annotations on main window. • Place text labels on the annotations. • View analysis results. • Change the color of the drawing.

Click the icon to show or hide the annotations window.

Layers of annotations display in different colors.

Layer Region displays data for each region within the Layer.

Page 12

WebScope Annotations Annotation Drawing Tools

• Annotations can define a region of interest and measure an object on the digital slide.

• To use a tool, select tool, left-click and move across the image.

Pen * Negative Pen*(excludes area) Ruler (measures in microns) Rectangle* Ellipse Arrow

*These annotation tools can be used to define regions to perform or exclude Image Analysis.

Tip: Right click to select an annotation tool.

Page 13

WebScope Annotations Layers and Attributes

• Each group of annotations can exist on a separate layer. • Organize information by pathologist or department.

• Each layer is given a separate color to easily differentiate one from another. • Each layer can have one or more attributes.

• Attributes can be text stored with the layer or analysis data.

Show/hide layers Change Color Add Layer Delete Selected Layer Delete All Layers

Layer Attributes displays combined image analysis results for all regions.

Page 14

WebScope Image Analysis Analyze a Digital Slide in WebScope

• Use the drawing tools to define an area of interest (unless doing whole slide analysis).

• Click on the main toolbar to open the Algorithm job window. • Select a macro, region of analysis and if a markup image is to be generated and click

Analyze.

A status message informs the job status.

Page 15

WebScope Image Analysis Analysis Results

• Quantitative results are available in a new layer in the Annotations window.

Page 16

WebScope Image Analysis Markup Image

• If requested, a markup image will be displayed with the digital slide.

For information on the meaning of the different pseudo-colors used in the markup, see the user’s guide for the particular algorithm you are using.