Image-Pro PremierBasic Training Course

Part 5 - Automated Counting

Automated Counting

Manual measurements take time and are difficult to repeat reliably

Objects in images often share intensity characteristics that are different from background or other object

By identifying groups of pixels based on pixel information we can automatically measure objects

Automated Counting

1.Segment image

2.Set up counting option

3.Select measurement types

4.Count

5.Display and save/export data

Segmentation

Divide pixels from the image into segments corresponding to:

Objects or one or more class

Background

Image-Pro can automatically identify bright or dark objects

Manual segmentation give more flexibility

Automatically Segmentation

Go to Count/Size ribbon

In Segment section select Bright or Dark

Pixels identified according to the the selection are highlighted on the image

Counting Options

Display Options - Affect look of objects

Segmentation and Count Options - Affect how objects are measured

Connectivity

4 Connect 8 Connect

Only pixels sharing a side are touching

Any neighbouring pixel can be touching

Clean Borders

Clean Borders

% Surface Area - Partial objects not important, holes probably need to be ignored - Clean borders > None

Morphology - Only whole objects to be measured, holes likely part of the objects - Clean borders > All

Counting - Need to avoid double counting partial objects, 1/2 objects on borders - Clean borders > 2 Adjacent

Measurement Types

Same as manual measurements

Select measurements relevant for object type

Use measurements for filtering

Displaying Data

When objects are counted, measurement table is automatically shown

Use Measurements Histogram to show spread

Use classification to enhance display on Image

Exporting Data

Quick Save for Publication to save image with overlays

Quick Save for Analysis to save image with measurements for review later

Export function in Measurements table to save data to Excel, a text file or for importing into Premier