ISOLATION AND CHARACTERIZATION OF ACETYLATED XYLAN FROM

SUGARCANE BAGASSE

UFV

COST ACTION FP1105 SAN SEBASTIAN 26TH – 27TH MAY 2015

Danila M. de Carvalho , Antonio M. Abad, Francisco Javier V. Domingo, Mikael E. Lindström, Jorge Luiz Colodette, Olena

Sevastyanovacarvalho.danila@gmail.com

MOTIVATION

Sugarcane: Brazilian situation

Bagasse: Stalk

fraction after juice removal

• 659 million tons of sugarcane (2014/15)• 92 million tons of bagasse (2014/15)

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BAGASSE

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Chemical composition

OBJECTIVE

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This study aims to evaluate the chemical and structural characteristics of acetylated xylan isolated from sugarcane bagasse.

EXPERIMENTAL

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Ethanol/toluene 1:2

(0.2 % EDTA, 90 º, 1 h)

10 % PAA, pH 3.5, 85 ºC, 30 min

NaClO2*,75 ºC, 30 min

DMSO, 24 ºC, 24 h, N2 atm.

DMSO, 24 ºC, 24 h, N2 atm.

Xylan Xylan

Chemical characterization

Sawdust ˂ 35 mesh

Extractives removalDelignification

Xylan isolation (yield determination)

*388 mL H2O, 15 mL acetic acid, 72 mL sodium acetate 30 % (w/v), and 55 mL sodium chlorite

30 % (w/v)

Fourier transform infrared (FTIR) spectrometry, 1H nuclear magnetic resonance (NMR), and sugar analysis

RESULTS

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Yield of DMSO isolation (xylan/holocellulose)

Delignification method

NaClO2 EDTA/PAA

Isolation yield, % 0.85 5.70

EDTA/PAA-DMSO xylanNaClO2-DMSO xylan

Yield and sugar analysis

RESULTS

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Where: a) PAA-holocellulose, b) NaClO2-holocellulose; c) xylan isolated by DMSO from PAA-holocellulose; d) xylan extracted by DMSO from NaClO2- holocellulose.

1H NMR spectrum of bagasse xylan extracted by DMSO from PAA-holocellulose (solvent is DMSO-d6).

FINAL CONSIDERATIONS

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• Xylan was isolated by two methods – from PAA and NaClO2 holocelluloses;

• Xylan sample isolated by PAA-holocellulose contained higher amount of xylose;

• The structure of xylan was confirmed by FTIR and 1H NMR methods;

• More detailed information on structure will be provided by SEC and linkage analysis.

ACKNOWLEDGEMENTS

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• European Community's Seventh Framework Program FP7/2007-2013 under the grant agreement number KBBE-2009-3-244362 LignoDeco (EU/Brazil co-operation);

• Coordination for the Improvement of Higher Education Personnel (CAPES);

• National Council for Scientific and Technological Development (CNPq);

• Knut and Alice Wallenberg Foundation;

• Cost Action FP1105.

THANK YOU!