hydrogen-based microbial interactions for successful ......development and grow ce to ethene...
TRANSCRIPT
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Hydrogen-Based Microbial Interactions for Successful Bioremediation
Rosa Krajmalnik-Brown Environmental Protection and Restoration
Thrust leader Professor, Arizona State University
School of Sustainable Engineering and the Built Environment
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Chlorotehenes in Groundwater
1. The problem 2. Bioinspired and Biomediated solution 3. Some contributions from my lab towards
enhancing Bioinspired solution 4. New H2 based insight
www.biogeotechnics.org
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Chlorotehenes in Groundwater
U.S. Superfund Sites:
• More than 1000 sites
• 60% polluted with PCE/TCE
3Image: https://www.epa.gov/enforcement/map-superfund-enforcement-cleanup-work
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Bioinspired solution?
Halogenated Organics
Biogenic Geogenic Anthropogenic Microbial Dehalogenation
Halogenation
F- I-Cl- Br-
Organic Compounds
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Reductive dechlorination
• Bioremediation of PCE and TCE to ethene occurs under anaerobic conditions via reductive dechlorination with H2 as electron donor.
• Dehalococcoides mccartyi are the only microbes capable of detoxification of chlorinated ethenes to ethene.
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Development of fast dechlorinating cultures Development and grow CE to ethene Dehalococcoides cultures
Sediment
Anaerobic medium & nutrients
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7 Delgado et al., Plos One, 2014, 9
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mm
ol L
−1
Time (days) Delgado et al., Plos One, 2014, 9 8
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Bioagmentation on the same soil?
Cuzdrioara bioaugmentation
Carolina bioaugmentation
9Delgado et al., Plos One, 2014, 9
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FTFHS
Arc mcrA Dhc Geo
10
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Hydrogen ”demand” in the subsurface
X Objectives
1. Track H2 consumption in a variety of soils and sediments
2. Which electron accepting processes dominate under a regime of excess H2.
Image: https://clu-in.org/ 11
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12
15 soils and sediments
-NO3
2-Organic C SO4
COD Fe3+
Inorganic C
100 days
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Lessons learned
• Robust Enrichment process, scale up and application at the field scale.
• Inorganic carbon metabolism (acetogenesis and methanogenesis) dominated in most soils.
• Organic carbon and Humics can consume H2 and perhaps release it later for processes like reductive dechlorination.
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Thank you!
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Questions? [email protected]
krajmalnik.environmentalbiotechnology.org
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