how to use dasgip od4 module - cbr.ubc.ca · 1. assemble bioreactor (before autoclave) 1a.position...

How to use DASGIP OD4 module

Prepared by Sung-Hye GriecoSingle Cell Fermenter SuiteCentre for Blood ResearchUniversity of British Columbia2350 Health Sciences MallVancouver, BC, V6T 1Z3

(Office Tel): 604-827-4356(Emergency Cell): 604-836-6774(E-mail): [email protected](Website): www.cbr.ubc.ca/fermenter.htm

Updated 2008 Feb

Contents

1.Assembly of bioreactors (before autoclave)2.Connect cables to sensors3.Connect to computer4.Operation of module5.Start experiment and save data6.Retrieval of Data after experiment7.How to clean and maintain sensors

How to use DASGIP OD4 modu

UBC Centre for Blood Research: Fermentation Suite P 1/

1. Assemble bioreactor (Before autoclave)1. Assemble bioreactor (Before autoclave)

1A. Position OD sensor into head plate (replace the rubber septum). Port adaptor for Applikon head plate was attached.

1B. Cover the attached dust cap.

1C.Autoclave bioreactors (30 min cycle).

2. Connect cable to sensors2. Connect cable to sensors


UBC Centre for Blood Research: Fermentation Suite Page 2/

2A. Connect sensor cables to the respective ports of the “IN” column at the rear of the transmitter. CAUTION: NEVER CONNECT OD SENSOR CABLES to the I/O PORTS. THIS WILL DAMAGE THE SENSORS AND/OR TRANSMITTERS.

2B. Connect OD sensor with cables. (Red dots on both cables are aligned.

2A

2B

Applikonadaptors

3A. Put the OD4 modules at desired place (Pichia or E.coli station) and connect unit to power outlet using supplied grounded power cord. Turn the “racking power switch” Off.

5A

5B

5C

3. Connect cable to computer3. Connect cable to computer

3B. Connect cable to computer (fist e-mail from Falk Schneider)

1) Connect the DTP-In (module) rear connector with the serial cable came with the OD4 module.

2) Connect the cable with computer on serial port COM1.

No serial port was available so USB port (COM3) was used.

3) Power on the OD4 module (racking power switch).





3C. Install the software (DTP-Insight, DASGAP EasyAccess)Find software called “DASGIP DTP-Server” (Originally this software couldn’t be found. On Feb 13th 2008, Falk Schneider phoned and e-mailed the software (downloaded form website) and saved to memory sticks and now installed in computer and copies are burn into same CD with other software. Icon won’t show up until it is installed.)

3D.Open DTP-Insight (calibration).

COM1, COM2, COM3 appears(couple of seconds later)

OD4 (25) folder appears under the COMP3



Right click on OD4 (25) folderSelect [Show DTP statistics]Table like below will appear and numbers are frequently changing(Module is communicating with computer)

4. Operation of OD4 module4. Operation of OD4 module

4A. Check digital display:Press [AU] button to change display unitsAU: Absorption, I: Sensor current (nA), T: Temperature (°C)

4B. Zero Calibration:1) Calibration with module

Select channel (OD sensor #1,2, or 3) – Press [1] – [3]Zero calibration – Press [Calib]Diamond will appear (on going calibration)Cancel on going calibration – Press [break]

2) Calibration with software (DTP-Insight)Open DASGIP OD4 moduleClick Zero calibration (3 chosen)

Diamond will appear(3 sensors)

Zero calibration for 3 sensorsare completed



4C.Define Correlation Curves:

1) Activate Correlation Curve Editor – Press [back], [∧], [∨], or [enter].



2) Select Correlation Curve #1– Press [1]General parameters will appear:

Move cursor to [1]: TagnameSelect tagname to [OD] – Rotate the Selector knob.

Move cursor to [2]: DecisSelect decimal to [3] – Rotate the Selector knob.

ex) 1 (0.2), 2 (0.20), 3 (0.200)

Move cursor to [3]: TypeSelect type to [Polynom] – Rotate the Selector knob.

Curve#1



Move cursor to [4]: Parameter

Move cursor to [1]: p0Select number (OD) to [0.000] – Rotate the Selector knob.

Move cursor to [2]: p1Select number (OD) to [x.xxx] – Rotate the Selector knob.

Press [enter] and [back] to go back to main screen.

All the sensor was chosen with curve #1:

Unit : ODDecimals: 0.001

Check correlation curve numbers for each column (sensor):Press [parts]

5B. Minimize the DTP-Server window. Open DASGIP EasyAccesssoftware (save data).

“Searching for network” will appear(couple of seconds later)“COM3 (4): OD4 (25)” will appear.

5. Start experiment and save data5. Start experiment and save dataHow to use DASGIP OD4 module


5A. Open DTP-Server to verify COM-port setting by clicking “Settings”. First and last COM port should be 1 (or the one the serial cable is connected with which is 3 for E.coli station (F#5, #6, #7) and 4 for Pichia station (F#1, #2, #3).). If you have to change settings please start the DTP-server.

Update Intervals : 5Fist DTP COM Port: 3 (F#5, #6, #7) or 4 (F#1, #2, #3)Last DTP COM Port: 3 (F#5, #6, #7) or 4 (F#1, #2, #3)



1) Type interval time (Sec): 300 (5 min)2) Click the OD4 (25) green box

6. Retrieval of data after experiment6. Retrieval of data after experiment



6A. Close the DASGIP EasyAccess software. OpenC:/Program files/DASGIP/EasyAccessData are saved as CSV forms. Name of data are set asOD4 (25)-yyyymmdd-hhmmss.cvs

6B. Download into memory stick and open with Excel program.

7. How to clean and maintain sensors7. How to clean and maintain sensorsHow to use DASGIP OD4 module


how to use dasgip od4 module - cbr.ubc.ca · 1. assemble bioreactor (before autoclave) 1a.position...

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