how to assess welfare in genetically altered animals? · welfare assessment is line-specific...
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HOW TO ASSESS WELFARE IN GENETICALLY ALTEREDANIMALS?
Anne ZintzschAnimal Welfare Officer
… IN ANIMAL WELFARE LEGISLATION
USE OF GENETICALLY ALTERED LAB ANIMALS IN GERMANY
0
100.000
200.000
300.000
400.000
500.000
600.000
700.000
800.000
900.000
1.000.000
2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013
40 % of mice used in animal experimentswere transgenic (2013)
Mouse strains
STATUS QUO AT THE MDC
Animal reporting
77%
23%
non-harmful phenotype
with a potential harmfulphenotype
31%
58%
11%
not genetically altered
genetically altered non-harmfulphenotypegenetically altered with harmfulphenotype
LEGAL REQUIREMENTSFOR SEVERITYASSESSMENT
Zintzsch A et al. Guidelines on Severity Assessment and Classification of Genetically Altered Mouse and Rat Lines. Lab Anim. 2017 accepted, In press
• 7 m, 7f• Neonates, at weaning, adult every two month line-specific
RECOMMENDATIONS FOR A BASIC WELFARE ASSESSMENT
Grune B et al: Animal welfare: Rules for assessing pain in lab animals. Nature 2014; 512: 28.Bundesinstitut für Risikobewertung: Severity Assessment of genetically altered mice and rats - Version 2: Recommendation no. 002/2016 by the National Committee (TierSchG) 2016.
BASIC WELFARE ASSESSMENT - OBJECTIVES
nonemildmoderate
severe
actual
prospective
Refinement Share &Archive
EXAMPLES ON SEVERITY ASSESSMENT AND CLASSIFICATION
EXAMPLE I: SUDDEN CARDIAC DEATH
GUIDELINES ON SEVERITY ASSESSMENT AND CLASSIFICATION
Zintzsch A et al. Guidelines on Severity Assessment and Classification of Genetically Altered Mouse and Rat Lines. Lab Anim. 2017 accepted, In press
DECOMPENSATED HEART INSUFFICIENCY
GUIDELINES ON SEVERITY ASSESSMENT AND CLASSIFICATION
Zintzsch A et al. Guidelines on Severity Assessment and Classification of Genetically Altered Mouse and Rat Lines. Lab Anim. 2017 accepted, In press
EXAMPLE III: MICROPHTHALMIA, ANOPHTHALMIA
GUIDELINES ON SEVERITY ASSESSMENT AND CLASSIFICATION
IV The lack of more than one sense is considered to cause an impairment that should be classified as harmful phenotype V If the animals are kept in a constant environment.
Zintzsch A et al. Guidelines on Severity Assessment and Classification of Genetically Altered Mouse and Rat Lines. Lab Anim. 2017 accepted, In press
EXAMPLE IV: DYSTROPHIC EPIDERMOLYSIS BULLOSA
Nyström et al: Rat Model for Dominant Dystrophic Epidermolysis Bullosa: Glycine Substitution Reduces Collagen VII Stability and Shows Gene-Dosage Effect., PLoS ONE. 2013;8(5):e64243
neonatal
GUIDELINES ON SEVERITY ASSESSMENT AND CLASSIFICATION
Zintzsch A et al. Guidelines on Severity Assessment and Classification of Genetically Altered Mouse and Rat Lines. Lab Anim. 2017 accepted, In press
EXAMPLE V:OBESITY
GUIDELINES ON SEVERITY ASSESSMENT AND CLASSIFICATION
Zintzsch A et al. Guidelines on Severity Assessment and Classification of Genetically Altered Mouse and Rat Lines. Lab Anim. 2017 accepted, In press
Welfare assessment is line-specific according to prospective severity assessment and should be adapted to observations
Team approach – scientist, animal caretaker, AWO/AWB
Guidelines on severity assessment and classification as starting point and will be reviewed regularly according to your feedback
Transfer and archive severity assessments to support animal welfare and science!
TAKE HOME MESSAGE
DRFZDr. Elena NoeAstrid Puppe
DIfEDr. Reinhart KlugeDr. Stephanie Krämer (now MDC)
HU Dr. Monika Reißmann
CharitéDr. Hannah NicklesDr. Kristina Ullmann
MDCDr. Claudia GöseleDr. Boris Jerchow (now UKE)
Vetmed Uni ViennaProf. Thomas Rülicke
