hl-2-1513 en [sas-2]peramed.com/peramed/docs/1212_5060169855540_en.pdf · 2020. 11. 24. · > for...

English

SAS-2O P E R A T O R S M A N U A L

1 The SAS-21.1 Introduction 31.2 Informational Symbols 31.3 Precautions and Limitations 41.4 Safety Considerations 5

2 Installation2.1 Electrical Installation 62.2 Fluid Installation 7

3 Controls3.1 Mains Supply 83.2 Rear Panel 83.3 Front Panel 8

4 Operating Instructions4.1 Power up 94.2 Loading a gel 94.3 Program selection 94.4 Create or Edit a program 114.5 Enter or Change a program name 134.6 Abort a program 14

5 Maintenance5.1 Regular maintenance 155.2 Manually cleaning tank 155.3 Maintenance before storage 155.4 Gel holder 155.5 Cleaning spills 155.6 Replace fuse(s) 15

6 Troubleshooting6.1 Problem, Cause, Solution 17

7 Performance Specifications7.1 Specifications and Parameters 18

8 Appendix A:8.1 Pre-set Programs 19

Warranty Report 21

2

Contents

SAS-2 Operators Manual

English

1.1 Introduction

The SAS-2 is a laboratory instrument designed to automatically dry, stain, de-stain and dryHelena Biosciences Europe SAS-1 agarose products.

The SAS-2 incorporates 10 factory set programs which allow it to operate with the standardgels of Helena BioSciences Europe. It also has the ability to store up to 9 programs with user-defined variables.

This manual provides all necessary information for the setup and operation of the SAS-2including important information regarding safety and maintenance of the unit. To ensure safeand reliable operation, always operate the SAS-2 in accordance with the operator manual.

READ SECTIONS 1.3 (PRECAUTIONS AND LIMITATIONS) AND 1.4 (SAFETYCONSIDERATIONS) OF THIS MANUAL BEFORE ATTEMPTING INSTALLATION ANDOPERATION.

Helena BioSciences Europe is not responsible for injuries or damages caused by improperuse.

1.2 Informational Symbols

WarningThe operators manual should be consulted to avoid possible personal injury orinstrument damage.

Caution, hot surface!Indicates an area where the surface of a specific part may have a temperatureexceeding 60°C.

3

1 The SAS-2


English

1.3 Precautions and Limitations

To isolate the equipment remove the plug from the socket. The mains switch is consideredto be the primary disconnect device and should be easily accessible by the operator.

> The entire operators manual should be read and understood before attemptinginstrument operation.

> This instrument is designed for use with Helena BioSciences Europe products only.> Refer to the Instructions For Use supplied with each kit for correct gel orientation,

staining / destaining fluid preparation, and other information.> To prevent damage to the SAS-2 ensure the slits on top of the instrument are not

covered.> Ensure the fan at the rear of the unit is not covered and has adequate air circulation.> Keep flammable liquids and flammable vapors away from the instrument at all times.> Keep the gel holder installed in the stainer at all times.> Do not use 100% methanol as this can effect the performance of instrument

components.> Do not use abrasive cleaners on any of the instrument surfaces. Always isolate the

mains supply before cleaning up any spills with a soft tissue.> This piece of equipment must not be used in areas of high electromagnetic

phenomenon as temporary loss of function may occur. Contact Helena BiosciencesEurope for advice.

4


English

1.4 Safety Considerations

The SAS-2 is fully overload protected. However, the following guidelines should be observedto ensure safe operation of the unit.

> The SAS-2 must only be operated when connected to an earthed supply of the correctvoltage and frequency as indicated on the serial number label.

> For emergency shut-down, use either the ON/OFF (switch on the rear panel of thedevice), or switch off at the mains supply.

> Wait at least 5 seconds after stopping a run before handling output leads or connectedapparatus.

> Always make sure your work area is clean and dry before operating the SAS-2.> Whilst the unit is in operation the user should keep clear the area around the gel holder

lift mechanism and avoid placing objects in the top of the staining chamber.> If the equipment is used in a manner not specified by the manufacturer the protection

provided by the equipment may be impaired.

To avoid damage to the instrument:

> For proper ventilation, leave at least 10 cm of space behind the instrument and oneither side.

> Do not operate the SAS-2 in high humidity environments (>90%), or wherecondensation may occur.

> To avoid condensation, the unit should be left on between use during operation in acold room or in high humidity environments.

> Do not lubricate any parts of the instrument.> Never place objects on top of the instrument.> Keep flammable liquids and flammable vapors away from the instrument at all times.

5


English

Please verify upon receiving the SAS-2 that no damage has occurred during transit andconfirm that the following components and accessories are included:

Item No. Units Ref. No.

SAS-2 1 unit Cat. No. 1212 (230V) or Cat. No. 1213 (115V)

Operator manual, including Warranty report 1 unit (art. no. 0100.061)Spare fuses 2 units (art. no. 6000.160) or

(art. no. 6000.315)Power cord 1 unit (art. no. 7100.001) or

(art. no. 7100.002) or (art. no. 7100.006)

Gel holder 1 unit (art. no. 0030.311)Liquid containers 5 units 1.5L (art. no. 8036.007)

1 unit 2.5L (art. no. 8036.008)1 unit 5L (art. no. 8036.009)

Tubing 5 meters (art. no. 8002.410)Container holder 1 unit (art. no. 0030.323)

2.1 Electrical Installation

> Place the SAS-2 unit on a level bench. Keep the area around the instrument clear toensure correct ventilation.

> Select an environment free from excessive humidity, dust and large temperaturefluctuations. Allow the instrument to acclimatise to room temperature for a period ofapproximately 2 hours before operating.

> The power cord attachment module is located at the rear of the unit. Position the unitso this is easily accessible.

> Connect the unit to a suitable earthed mains outlet of the correct voltage andfrequency. This unit MUST BE EARTHED.

> Turn on the main power switch located on the left side of the instrument. A green LEDwill be activated on the service panel when the power is on.

6

2 Installation


English

2.2 Fluid Installation

> Connect the ports on the rear of the instrument to the relevant solutions. Use onlytubing and container sets provided with the instrument.

> All tubing must be securely connected to the fittings to prevent leaks.

7


English

Port 1

Port 2

Port 3

Port 4

Port 5

Port 6

Port 7 Waste

3.1 Mains Supply

ON/OFF Switch: This is a rocker-type switch which controls the mains input tothe unit.

3.2 Rear Panel

Ports: 6 x Inlet ports.1 x Outlet port.

3.3 Front Panel

Display Unit: LCD operator interface providing information of program parameters and machine status.

LED: Indicates that the current is flowing between the electrodes.

Enter: Press to access program parameters.Press to access and alter individual steps.

Left Arrow: Scroll cursor left.

Right Arrow: Scroll cursor right.

Up Arrow: Scroll by increments through steps.Increase parameter values.

Down Arrow: Scroll by decrements through steps.Decrease parameter values.

Start/Stop: Start program / Abort program during run.

8

3 Controls


English

4.1 Power up

Connect the unit to an appropriate mains outlet and switch on by using the power switchlocated on the rear of the instrument. The display will prompt:

Helena BioSciencesStaining Unit

This shows that the machine is ready to operate.

4.2 Loading a gel

The gel holder has a front and a back, see Figure 1. The gel must be loaded from the frontside. First ensure that the right-hand side variable bar is at the correct setting for the gel.This can be adjusted by first loosening the nut on the rear of the bar and then moving the barto the correct location holes before tightening. Ensure that the movable discs A and B arepushed upwards against the handle.

Insert the gel (gel side facing front) into the groove of the fixed left hand bar. Gently bendthe gel to the front to allow location of the right-hand edge into the groove of the variablebar. Move the gel down to locate it against the fixed discs C, D, E and F. Move the discs Aand B down to hold the gel in place.

Insert the gel holder into the top of the staining compartment of the instrument. The gelsurface should be facing forward with the variable bar to the right-hand side. The holdershould slide into the grooves with ease. DO NOT force the holder in. If the holder issticking, remove and relocate.

4.3 Program Selection

Press any key and the display will prompt.

Select a ProgramPr 1 SP-24

Use the ‘up’ and ‘down’ arrow keys to scroll through the program menu. Programs 1 to 10are factory set. A full overview of these is given in Appendix A. Programs 11 to 19 arecustomer set.

9

4 Operating Instructions


English

Figure 1

10


English

Variable Discs A and B

Variable Bar

Fixed Discs C, D, E and F

Front

Back

Location nut

4.4 Create or Edit a program

To start the program, press the ‘Start’ button.

From the ‘select a program’ prompt use the ‘up’ and ‘down’ keys to select a program or anempty program slot.

Select a ProgramPR 15

Use the ‘Enter’ key to enter the program, the prompt will change to:

Entering Program 15

and then to,

Pr 15 Step 1NO FUNCTION

PR 16 Prot 1 Step1WASH 1 30:00 1

The function of each section of the display is:


11


English

Program Name

Program Number

Function of current step

Port used during step

Current step of program

Time of activity

Number ofrepeats of step

12


English

Press the ‘Enter’ key once more to enter an individual step. A cursor should appear over the1 of ‘Step 1’.


Use the ‘left’ and right’ arrow keys to move the cursor over the first letter or number of thefunction. Use the ‘up’ and ‘down’ arrow keys to select the function of the step.

Possible functions are: No functionDryFixStainDestainClearWash

Use the ‘left’ and ‘right’ keys to move to the port selection number and alter this to thecorrect port using the ‘up’ and ‘down’ arrow keys. With the drying function the temperatureis stated here. Move to the time value and set to the appropriate time. Then move to therepeat value and set the number of repeats required (up to a maximum of 9). Repeats cannotbe set at greater than 1 for drying steps. Use the ‘left’ and ‘right’ keys to return the cursorover the step number. Use the ‘up’ and ‘down’ keys to move to the next step. Repeat theabove sequence until all of the steps are entered.

To save the program press the ‘Enter’ key and the prompt will change to:

Saving ProgramPlease wait

then to


Press the ‘Start/stop’ key to exit to the program selection prompt.

4.5 Enter or Change a program name

From the program selection prompt.

Select a ProgramPr 15

Press the ‘left’ arrow key, the prompt will change and the cursor will appear over the firstcharacter of the program name.

Enter Program namePr 15

Use the ‘up’ and down’ arrow keys to select the letter required. Use the ‘left’ and ‘right’arrow keys to select the next letter to be changed. Proceed until the program name iscomplete.

Press the ‘Enter’ key to save and the prompt will change to:

Saving Program namePlease wait

And then return to the program selection prompt.

13


English

4.6 Abort a Program

At any point during a run a program may be aborted by pressing the ‘Stop’ key. The promptwill change to:

Aborting the ProgramPlease wait

The program will hold, empty the chamber and enter into the chamber cleaning process.After this cleaning of the chamber the prompt will change into:

Press STOP to abortENTER to continue

When the ‘Stop’ key is pressed, the program will return to stand by mode. When ‘Enter’ ispressed the last step will be repeated and the program continues from that point on.

It is not possible to stop the cleaning process at the end of each run, as it is required forinstrument maintenance.

14


English

This section describes routine operators maintenance procedures. NOTE: No operator adjustments are necessary. There are no user serviceable or adjustable parts inside theunit. Any service problems or adjustments should be referred to your nearest Helena BioSciences EuropeDistributor or Service Department.

5.1 Regular MaintenanceAt the end of each session ensure that port 3 is connected to either a 50/50 methanol/watersolution or the 5:5:1 fixative solution. Select program 10 (clean). The empty gel holdershould be in the instrument to facilitate a good cleaning process.

5.2 Manually cleaning tankSoak the brush provided in a 50/50 Methanol/water solution. Clean the tank as shown inFigure 2 below, pay particular attention to the left side (front view) of the tank as this is wherethe level detectors are situated.

5.3 Maintenance before storageBefore storage of the instrument for long periods of time connect all ports to either a 50/50methanol/water solution or the 5:5:1 fixative solution. Select program 9 (service). The empty gel holder should be in the instrument to facilitate a good cleaning process.Following the use of program 9 with methanol containing solution, the program should berepeated with all ports connected to deionised water.

5.4 Clean Gel HolderWipe off all residues on the gel holder with a cloth soaked in destain, rinse in deionised waterand dry with a lint-free tissue. For heavy accumulations, soak gel holder in destain untilresidue can be removed, then rinse in deionised water and dry.

5.5 Cleaning SpillsClean spills with a soft cloth or sponge ONLY after turning off the power and unplugging thepower cord. Do not use corrosive cleansers. Dry the unit before plugging in the power cord.

5.6 Replace fuse(s)1. Switch off the device and remove the mains cable from the input.2. Withdraw the fuse carrying tray from the fuse holder and remove the fuses.3. Replace with fuses of the correct type and rating.4. Replace fuse holder5. Plug power cord back into the instrument and switch on the power6. If fuses blow immediately contact Helena BioSciences Europe for assistance.

15

5 Maintenance


English

Figure 2

16


English

NOTE: If problem persists or any other messages are displayed on the screen, contactHelena BioSciences Europe for assistance.

17

6 Troubleshooting


English

Problem Cause Solution

No power to the instrument

Power cord not connected Check power cordconnections at both ends

Fuse(s) blown Replace fuse(s)

Power on/off is off Switch on/off switch ‘on’

Gel not clean Destain and washprocedure has not beenperformed correctly

Check program steps

Check Destain / Wash timesettingCheck bottle contents

Check port connections

Gel not dry Drying procedure has notbeen performed correctly

Check program steps

Check dry time setting

Check dry temperature

Poor staining results Staining procedure has notbeen performed correctly

Check program steps

Check stain time setting

Check bottle contents

Check port connections

Error message ‘Clean tank’ Tank is dirty Clean tank

Display: 2 x 20 character LCD

Keypad: 6 keys

Operating Modes: Standby, Program selection, Program editing and Program run.

Input Power: 230 Vac (Cat. No. 1212)115 Vac (Cat. No. 1213)Frequency 50/60 Hz, 380 wattsVoltage is factory set (see Serial No. Label)

Timers: Set value: from 0 to 99 minutesfrom 0 to 59 seconds

Accuracy: Better than +/- 1 % of setting.

Dry temperature: Set value: 60, 65 or 70°CAccuracy: Better than +/- 2°C

Fuse - Mains: 230 Vac 1.6A T 5 x 20mm.115 Vac 3.15A T 5 x 20mm.2 fuses required (live and neutral fused)

Safety Interlock: Heating element power breaks if dry-fan is defective.

RS232: Not implemented yet

Dimensions: Weight: 9 kgSize: 24 cm x 36 cm x 30 cm (WxDxH)

18

7 Performance Specifications


English

1. SP-24

Step Temp Port Time (mm:ss) Repetitions

Fix Port 3 05:00Dry 65°C 15:00Stain Port 6 10:00Destain Port 2 02:00 Repeat x 2Wash Port 1 01:00Dry 65°C 15:00

2. IFE-4


Dry 65°C 15:00Stain Port 5 10:00Destain Port 2 02:00 Repeat x 3Wash Port 1 01:00Dry 65°C 15:00

3. Acid Hb


Fix Port 4 05:00Stain Port 5 15:00Destain Port 4 00:10Dry 65°C 15:00Destain Port 4 02:00 Repeat x 2Wash Port 1 01:00Dry 65°C 15:00

4. Alk Hb


Fix Port 3 05:00Dry 65°C 15:00Stain Port 6 10:00Destain Port 2 02:00 Repeat x 3Wash Port 1 01:00Dry 65°C 15:00

19

8 Appendix A: Pre-set programs


English

5. Ur. Scr

Step Temp Port Time (mm:ss) RepetitionsFix Port 4 05:00Dry 65°C 15:00Stain Port 5 10:00Wash Port 1 00:10 Repeat x 2Destain Port 2 02:00 Repeat x 2Wash Port 1 00:30Dry 65°C 15:00

6. Ur. IFE

Step Temp Port Time (mm:ss) RepetitionsDry 65°C 15:00Stain Port 5 10:00Destain Port 2 02:00 Repeat x 2Wash Port 1 01:00Dry 65°C 10:00Destain Port 4 01:00Dry 65°C 10:00

7. Reserved

8. Service

Step Temp Port Time (mm:ss) RepetitionsWash Port 6 00:10 Repeat x 2Wash Port 5 00:10 Repeat x 2Wash Port 4 00:10Wash Port 3 00:10Wash Port 2 00:10Wash Port 1 00:10

9. Clean

Step Temp Port Time (mm:ss) RepetitionsDestain Port 3 05:00 Repeat x 2

20


English

Please complete and return to Helena BioSciences Europe, by email, fax or post.

Instrument Name:

Date Received: Catalogue Number:

Serial Number:

Name: Department:

Institution:

Address:

Post Code:

Telephone Number:

Ext. (where applicable):

Email:

WarrantiesHelena warrants each unit to be free from defects in material and workmanship. Helena’s liability under thiswarranty is limited to servicing or adjusting any unit and to replacing any defective part. This warranty iseffective for 1 year commencing from the date of shipment to the original purchaser, and does not cover faultscaused by misuse, abnormal conditions, damage incurred in shipment, or consumable items such as lamps. This warranty is made in lieu of all other warranties, expressed or implied, and is limited in any sale to the repairor replacement of the affected component.

21

Warranty


English

Condition of Equipment on Arrival

In order to evaluate shipping procedures and conditions of equipment on arrival, pleasecomplete this report and return to us.

1. Did the instrument arrive in apparent good condition?

Yes [ ] No [ ]

2. Will you be able to operate this instrument without additional information?

Yes [ ] No [ ]

Comments:

Helena BioSciences Europe is committed to providing you with the finest equipment and supplies possible. If you need assistance please call our Sales / Service Department:

tel: +44 (0) 191 482 8440fax: +44 (0) 191 482 8442

email: [email protected]

22


English

HL-2-1513P 2006/06 (2)

Helena BioSciences EuropeQueensway SouthTeam Valley Trading EstateGatesheadTyne and WearNE11 0SD

tel: +44 (0)191 482 8440fax: +44 (0)191 482 8442

email: [email protected]

/ColorImageDict > /JPEG2000ColorACSImageDict > /JPEG2000ColorImageDict > /AntiAliasGrayImages false /DownsampleGrayImages true /GrayImageDownsampleType /Bicubic /GrayImageResolution 350 /GrayImageDepth -1 /GrayImageDownsampleThreshold 1.50000 /EncodeGrayImages true /GrayImageFilter /DCTEncode /AutoFilterGrayImages true /GrayImageAutoFilterStrategy /JPEG /GrayACSImageDict > /GrayImageDict > /JPEG2000GrayACSImageDict > /JPEG2000GrayImageDict > /AntiAliasMonoImages false /DownsampleMonoImages true /MonoImageDownsampleType /Bicubic /MonoImageResolution 1200 /MonoImageDepth -1 /MonoImageDownsampleThreshold 1.50000 /EncodeMonoImages true /MonoImageFilter /CCITTFaxEncode /MonoImageDict > /AllowPSXObjects false /PDFX1aCheck false /PDFX3Check false /PDFXCompliantPDFOnly false /PDFXNoTrimBoxError true /PDFXTrimBoxToMediaBoxOffset [ 0.00000 0.00000 0.00000 0.00000 ] /PDFXSetBleedBoxToMediaBox true /PDFXBleedBoxToTrimBoxOffset [ 0.00000 0.00000 0.00000 0.00000 ] /PDFXOutputIntentProfile (None) /PDFXOutputCondition () /PDFXRegistryName (http://www.color.org) /PDFXTrapped /Unknown

/Description >>> setdistillerparams> setpagedevice

hl-2-1513 en [sas-2]peramed.com/peramed/docs/1212_5060169855540_en.pdf · 2020. 11. 24. · > for...

Documents