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New Senior Secondary Mastering Biology Second Edition

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  • Contents

    1 Introduction to HKDSE 1

    Part I Public examination

    2 Types of questions in the HKDSE exam paper 4

    3 General exam skills 6

    4 Skills in answering MCQs 10

    5 Skills in answering essay questions 11

    6 Skills in plotting graphs 13

    7 Skills in drawing biological diagrams 14

    8 Skills in studying biology 16

    Part II School-based assessment (SBA)

    9 About SBA 20

    10 Basic experimental techniques 21

    11 Skills in writing investigation reports 25

  • New Senior Secondary Mastering Biology 1 Oxford University Press 2014 (Second Edition)

    HKDSE (Hong Kong Diploma of Secondary Education) examination is a public assessment aiming

    to assess the attainment of candidates who have completed the 3-year senior secondary course.

    A Mode of assessment

    HKDSE examination in Biology and Combined Science (Biology) comprises two components:

    public examination and school-based assessment (SBA).

    Biology

    Public examination SBA

    Paper 1 Paper 2

    Curriculum coverage Compulsory part Elective part Whole

    Assessment tasks Section A: Multiple-choice questions

    (36 marks)

    Structured questions

    (40 marks)

    Practical related

    tasks

    Section B: Short questions, structured

    questions and an essay

    question (84 marks)

    Number of questions

    to be attempted All 2 out of 4

    Duration 2 h 30 min 1 h S5 & S6

    Weighting 60%

    (Section A: 18%; Section B: 42%) 20% 20%

    Combined Science (Biology)

    Public examination (one paper only) SBA

    Curriculum coverage Whole Combined Science (Biology) Whole

    Assessment tasks Section A: Multiple-choice questions (24 marks) Practical related

    tasks Section B: Short questions, structured questions and an essay

    question (56 marks)

    Number of questions

    to be attempted All

    Duration 1 h 40 min S5 & S6

    Weighting 40% (Section A: 12%; Section B: 28%) 10%

    11 IInnttrroodduuccttiioonn ttoo HHKKDDSSEE

  • New Senior Secondary Mastering Biology 2 Oxford University Press 2014 (Second Edition)

    B The reporting system

    HKDSE makes use of standards-referenced reporting (SRR) () of assessments. The

    candidates level of performance will be reported as five levels (15), with 5 being the highest.

    A performance below Level 1 will be designated as Unclassified (U). The Level 5 candidates with

    the best performance are awarded a 5**, and the next top group of candidates are awarded a 5*.

    Each level has a set of descriptors () to describe what a typical candidate at this level is

    able to do. Details of the descriptors and samples of candidates performance of different levels in

    the past HKDSE examinations can be found at the HKEAAs website.

    http://www.hkeaa.edu.hk/en/hkdse/assessment/subject_information/ category_a_subjects/hkdse_subj.html?A2&2&5

    1

    2

    3

    4

    5

    5*

    5**

  • New Senior Secondary Mastering Biology 3 Oxford University Press 2014 (Second Edition)

    PPaarrtt II

    PPuubblliicc eexxaammiinnaattiioonn

  • New Senior Secondary Mastering Biology 4 Oxford University Press 2014 (Second Edition)

    1 Multiple-choice questions (MCQs)

    For Biology, there will be 36 MCQs in Paper 1 Section A.

    For Combined Science (Biology), there will be 24 MCQs in Section A of the paper.

    Each question scores 1 mark.

    2 Short questions

    Each short question scores about 27 marks.

    There are various types of short questions. Some examples are shown below.

    a Fill-in-the-blanks

    b Matching

    In addition to the above types of short questions, some short questions begin with diagrams,

    photographs, graphs or tables of data, which are followed by a few questions.

    Column 1 Column 2

    Mitochondrion ________ A. Encloses the cytoplasm

    B. The site of energy release during respiration

    Nucleus ________ C. Controls activities of the cell

    D. Supports and gives shape to the cell

    Cell wall ________ E. Contains organelles

    22 TTyyppeess ooff qquueessttiioonnss iinn tthhee HHKKDDSSEE eexxaamm ppaappeerr

    SSaammppllee qquueessttiioonn

    Amino acids are made up of carbon, hydrogen, oxygen and (a) ______________. Each amino

    acid molecule contains an amino group and a (b) _____________ group. A (c) _____________

    is formed when two amino acids combine together. It can combine with more amino acids to form

    a long chain called the (d) ______________ .

    SSaammppllee qquueessttiioonn

    For each cell structure listed in column 1, select from column 2 one phrase that correctly

    describes its function. Put the appropriate letter in the space provided. (3 marks)

  • New Senior Secondary Mastering Biology 5 Oxford University Press 2014 (Second Edition)

    3 Structured questions

    Each structured question scores about 712 marks.

    Structured questions are usually divided into parts (a), (b) and (c), etc. Each part may be

    divided into smaller parts (i), (ii) and (iii), etc.

    4 Essay questions

    For both Biology and Combined Science (Biology), there will be an essay question in the exam

    paper. It scores about 1012 marks.

    In the total mark of the essay, 3 marks are awarded for effective communication.

    SSaammppllee qquueessttiioonn

    The diagram below represents a cross section of a flower.

    (a) Name structure P and state its function. (2 marks)

    (b) Using the letters in the diagram, state where

    (i) meiotic cell division takes place. (2 marks)

    (ii) fertilization takes place. (1 mark)

    (c) State the fate of structures P, Q, R, S and U after fertilization. (5 marks)

    SSaammppllee qquueessttiioonn

    Compare the prophase in mitosis and first meiotic division. In what way does the difference

    in the prophase of first meiotic division affect the products of meiotic cell division?

    (11 marks)

  • New Senior Secondary Mastering Biology 6 Oxford University Press 2014 (Second Edition)

    1 Time allocation

    * depends on the mark of each question

    Do the easiest questions first to build up your confidence. Do not spend too much time on

    answering a difficult question. Either give it up or save it for the last.

    2 Understanding command words in questions

    Before answering a question, you should look for the command word in the question. It tells you

    what you should answer. Below are some common command words in exam questions.

    Command word Point to note Example of exam question

    Calculate () Remember to show the working steps and give the answer a right

    unit.

    According to the information given,

    calculate the rate of water absorbed by

    the plant.

    Comment () You need to present an informed opinion.

    Comment on the significance of the

    relationship between the structures for

    gas exchange and water transport in

    terrestrial flowering plants.

    Subject Biology Combined Science (Biology)

    Exam paper Paper 1

    Section A

    Paper 1

    Section B Paper 2 Section A Section B

    Mark 36 marks 84 marks 40 marks 24 marks 56 marks

    Duration 2 h 30 min 1 h 1 h 40 min

    Suggested

    time

    allocation

    Answering whole

    section 30 min 1 h 40 min 50 min 20 min 65 min

    Checking whole

    section 5 min 15 min 10 min 5 min 10 min

    Answering ONE

    question 50 s 310 min* 25 min 50 s 310 min*

    33 GGeenneerraall eexxaamm sskkiillllss

  • New Senior Secondary Mastering Biology 7 Oxford University Press 2014 (Second Edition)

    Command word Point to note Example of exam question

    Compare () Give both similarities and differences. Try to use

    comparative words like larger,

    smaller, etc.

    Compare the structures of a bacterial

    cell and a yeast cell.

    Contrast () Give the differences only. Contrast the processes of mitosis and meiosis.

    Deduce () Deductions must be made based on the information given.

    Based on the results of the cross,

    deduce which phenotype is recessive.

    Describe () No explanation is required. Describe how insulin can bring about a decrease in blood glucose level.

    Determine () Your answer should be based on the information given.

    According to the data given, determine

    the concentration of the insecticide at

    which 30% of mosquitoes are killed.

    Discuss () Give arguments for and against an issue and provide explanations.

    Discuss the pros and cons of

    producing human insulin by genetic

    engineering.

    Distinguish () Give the difference between two things.

    Distinguish between food-borne

    infection and food poisoning.

    Explain / Account for () Give reasons. Explain / Account for the increase in the length of a potato strip that has

    been put into distilled water for one

    hour.

    Give / State () No explanation is required. Give / State two functions of proteins in the human body.

    Identify () Give the name and make sure the spelling is correct.

    Using the dichotomous key given,

    identify animals A and B.

    Label () Make sure the spelling is correct. Label the cells shown in the photomicrograph.

    Name () Make sure the spelling is correct. Name the type of cell division occurring in the gamete-producing

    cells in human ovaries.

    Outline () No detail is required. Describe the major steps in recombinant DNA technology and

    outline their principles.

    Suggest () Put forward your ideas. Suggest two preventive measures that we can take to reduce the risk of

    contracting dengue fever.

  • New Senior Secondary Mastering Biology 8 Oxford University Press 2014 (Second Edition)

    3 Following the instructions

    You must follow the instructions to answer the questions. Below are some examples of instructions.

    Instruction What to do?

    Using the letters in the diagram, Answer with the letters provided in the

    diagram, not the names.

    With reference to the photomicrograph, Use the photomicrograph / diagram provided

    to arrive at the answer. Referring to the diagram,

    State one other cause of Give another cause that is different from the

    one stated in the question.

    Use a genetic diagram to show the result. You must include a genetic diagram.

    Explain your answer without using a genetic diagram. On the contrary, DO NOT use a genetic

    diagram to arrive at your answer. Deduce, with reasons, the genotype of

    Marks will not be awarded for genetic diagrams.

    4 Communicating effectively

    In answering structured questions and essay questions, express your ideas systematically and

    logically. Make good use of linking words to link different ideas.

    For example:

    Command word Linking words

    Describe (about a graph) Between and / From to

    Outline the steps Firstly Secondly Thirdly Finally

    Give examples of For example / This includes / such as

    Contrast however / while / whereas /

    In contrast / On the contrary

    Explain / Account for Since / Because So / Hence / Thus / Therefore / As a result

  • New Senior Secondary Mastering Biology 9 Oxford University Press 2014 (Second Edition)

    5 Avoiding common mistakes

    a Spelling mistakes

    When doing fill-in-the-blanks, or when you are asked to name, identify or label a

    certain structure, correct spelling is required. The key words in long answers must

    also be spelt correctly. Below are some commonly misspelt words.

    Correct Wrong Correct Wrong

    amnion amino nuclei nucles

    dietary fibre diary fibre pancreas pancrease

    gastric juice gastic juice phloem pholem

    membrane membrance prey pray

    night blindness night blindless vertebrate vertibrate

    b Inappropriate use of words

    Do not confuse words with similar spellings or pronunciations. For example, the pupil of the

    eye constricts, not contracts, under strong light.

    c Omitting titles, headings or units

    Remember to give your drawing, graph or table an appropriate title or heading. For

    calculation, assign a correct unit to the value obtained.

    Under strong light,

    the pupil contracts.

    the pupil constricts.

  • New Senior Secondary Mastering Biology 10 Oxford University Press 2014 (Second Edition)

    1 If you are CERTAIN with the answer

    put a beside the question and then skip the question for checking.

    2 If you are UNCERTAIN with the answer

    put a ? beside the question to remind

    you to pay attention during checking.

    look for opposing answers. It is very likely that one of them is the correct answer.

    cross out answers that mean basically the same thing. They cannot both be correct.

    make a guess instead of leaving the answer blank.

    44 SSkkiillllss iinn aannsswweerriinngg MMCCQQss

  • New Senior Secondary Mastering Biology 11 Oxford University Press 2014 (Second Edition)

    There are four major steps to help you answer essay questions.

    Step 1 Read the question carefully

    While you are reading the question, underline the key points and circle all the command words

    (e.g. compare, explain, etc.). This can remind you what should be included in your essay.

    Step 2 Organize your ideas

    Use a mind map, a flow chart or a table to organize your ideas. When it is finished, check if the

    ideas are coherent and systematic. Cross out any irrelevant information.

    Lipids Proteins

    Similar functions - components of the cell membrane

    - for making hormones

    - produce ATP

    - as energy reserves

    - for protection

    Different functions - transport lipid-soluble vitamins

    - as an insulator

    - for making myelin sheath

    - as enzymes

    - as antibodies

    - as haemoglobin

    - for blood clotting

    SSkkiillllss iinn aannsswweerriinngg eessssaayy qquueessttiioonnss

    Q SSaammppllee qquueessttiioonn

    Lipids and proteins are two primary food substances which are essential

    to life. Compare their functions in the human body.

    55

  • New Senior Secondary Mastering Biology 12 Oxford University Press 2014 (Second Edition)

    Step 3 Write your essay

    Divide your essay into three parts:

    Step 4 Check your essay

    Check if the essay includes the necessary information. Make sure there are no spelling mistakes or

    inappropriate use of words.

    Lipids and proteins have many functions in the human body.

    Some of the functions are similar but some are unique to each

    type of biomolecule.

    Both lipids and proteins are components of the cell

    membrane. They are used for making hormones such as

    steroids (lipids) and insulin (proteins). Both of them act as

    energy reserves and can be broken down to produce ATP.

    Lipids and proteins also offer protection to the body. For

    example, adipose tissues around the internal organs can act as

    a shock absorber and keratin in nails can provide mechanical

    protection.

    Lipids are a solvent for some vitamins (e.g. vitamins A and D).

    They are a good insulator to reduce heat loss from the body.

    Lipids are also used for making myelin sheath around nerve

    fibres to speed up the transmission of nerve impulses.

    Some proteins are enzymes. They can catalyse the biochemical

    reactions in the body. Proteins can act as antibodies which act

    against the pathogens invading the body. Some proteins are

    transporters such as the oxygen carrier haemoglobin. Fibrous

    proteins are involved in blood clotting. They form a network

    to trap the red blood cells.

    Lipids and proteins have some similar but also some different

    functions in the human body. They are both important

    biomolecules.

    1 Introduction

    Briefly describe the main

    theme of your essay.

    Avoid too much

    background information.

    2 Body

    Write in paragraphs. Each

    paragraph should have a

    main point and the point

    is explained, elaborated or

    supported by evidence.

    3 Conclusion

    Sum up your points and

    restate the main theme.

  • New Senior Secondary Mastering Biology 13 Oxford University Press 2014 (Second Edition)

    Note the following when plotting a graph:

    1 Use the x-axis to denote the independent variable and y-axis to denote the dependent variable.

    2 Choose a suitable scale and make full use of the graph paper.

    3 Label each axis and show the units.

    4 Plot each point as a small cross or dot with a sharp pencil.

    5 Join the points. If two or more lines are to be drawn on the same graph, draw with different

    styles (e.g. solid line versus dotted line) and label them.

    6 Give a title to the graph.

    EExxaammppllee

    In an experiment, rats from the same mother were divided into groups A and B. Group A was fed on purified

    cheese proteins, glucose, starch, fats, minerals and water. Group B was fed on the same food with additional

    3 cm3 of milk each day. After 20 days, the diets of the two groups were exchanged. The average mass of the

    two groups of rats from day 0 to day 50 was recorded. The results are presented on the graph below.

    66 SSkkiillllss iinn pplloottttiinngg ggrraapphhss

    symbol to represent the unused part of the scale (045 g). This makes the graph easier to read

    5 10 15 20 25 30 35 40 45 50 time (day)

    45

    50

    55

    60

    65

    70

    75

    80

    85

    90

    0

    average mass

    of rats (g)

    The change in average mass of rats against time

    group A

    group B

    y-axis is labelled and unit is shown

    x-axis is labelled and unit is shown

    title is given

    graph paper is fully used with a suitable scale

    two curves are drawn with different styles and labelled

  • New Senior Secondary Mastering Biology 14 Oxford University Press 2014 (Second Edition)

    The diagram below is poorly drawn. Can you point out the mistakes?

    Points to note when drawing biological diagrams

    1) Use a sharp HB pencil. Never draw in pen.

    2) Draw large diagrams, occupying about two-third of a piece of A4 paper.

    3) Draw with smooth single lines. Keep all parts in proportion.

    4) Do not shade the diagrams. If you need to show the contrast, you may only use dots.

    The denser the dots are, the deeper is the colour.

    5) Draw freehand. Do not use a ruler or a pair of compasses.

    6) Draw what you see from the specimen. Do not copy from books.

    7) Label different parts of the drawing.

    Put the labels by the sides, using labelling lines.

    Labelling lines should be straight, horizontal and should not cross each other.

    8) Give a title, including the name of the specimen and the power of magnification.

    9) State how the section is cut if you are drawing a cut surface of a specimen, such as

    longitudinal section (L.S.) or transverse section (T.S.).

    10) State the direction of view if you are drawing a whole specimen, such as dorsal,

    ventral or lateral view.

    SSkkiillllss iinn ddrraawwiinngg bbiioollooggiiccaall ddiiaaggrraammss 77

  • New Senior Secondary Mastering Biology 15 Oxford University Press 2014 (Second Edition)

    There are two kinds of microscopic drawings:

    1 Low-power diagrams

    a They show the distribution and proportion of different tissues.

    b Only outlines of structures are drawn. No need to draw individual cells.

    For example:

    Dicotyledonous plant root (T.S.) (10)

    2 High-power diagrams

    a They show the features of different types of cells.

    b Draw individual cells.

    For example:

    Vascular tissue in dicotyledonous plant root (T.S.) (400)

  • New Senior Secondary Mastering Biology 16 Oxford University Press 2014 (Second Edition)

    Studying biology can be easy if you understand the biological concepts instead of memorizing

    them by rote. Knowing some basic techniques can help you learn biology more effectively.

    1 How to remember the spellings of biological terms more easily?

    a By understanding prefixes and suffixes

    Many biological terms are built up on Greek or Latin prefixes () and suffixes (). By

    dissecting the biological terms into discrete units and knowing the meanings of the prefixes and

    suffixes, you will remember their meanings and spellings more easily.

    For example:

    Prefix Meaning Example

    an- lacking, without anaerobic (), anaemia ()

    anti- against antigen (), antibody (), anticodon ()

    auto- self autotroph (), autoimmune disease (),

    autonomic nervous system ()

    bi- two biceps (), bicuspid valve (), binary fission ()

    di- two disaccharide (), dicot (),

    dihybrid ()

    endo- within endocrine gland (), endolymph ()

    exo- outside exocrine gland (), exoskeleton ()

    haem- blood haemoglobin (), haemolysis ()

    hyper- above hypertonic (), hypertension ()

    hypo- below hypotonic (), hypothermia ()

    mono- one monosaccharide (), monocot (),

    monoculture ()

    photo- light photosynthesis (), phototropism (),

    photolysis ()

    tri- three triceps (), tricuspid valve (),

    triglyceride ()

    SSkkiillllss iinn ssttuuddyyiinngg bbiioollooggyy 88

  • New Senior Secondary Mastering Biology 17 Oxford University Press 2014 (Second Edition)

    Suffix Meaning Example

    -ase enzyme amylase ( ), invertase ( ), lipase ( )

    -cide killing agent fungicide (), herbicide (), pesticide ()

    -lysis breaking down glycolysis (), hydrolysis (), photolysis ()

    -plast living granule chloroplast (), protoplast ()

    -trophic feeding autotrophic nutrition (),

    heterotrophic nutrition ()

    b By pronouncing words correctly

    Marking down each syllable () of a word with slashes is very helpful for memorizing the

    spelling. For example:

    accommodation ac / com / mo / da / tion

    assimilation as / si / mi / la / tion

    epididymis e / pi / di / dy / mis

    homeostasis ho / me / o / sta / sis

    metabolism me / ta / bo / li / sm

    Bear in mind that this is only a way for you to remember the spellings of the words, but not a

    way for you to acquire the correct pronunciations.

    c By distinguishing words with similar spellings

    Make sure you are able to distinguish words with similar spellings. For example:

    amino amnion

    afferent efferent

    cerebrum vs cerebellum

    glycogen glucagon

    ovule ovary

    vertebrate vertebra

  • New Senior Secondary Mastering Biology 18 Oxford University Press 2014 (Second Edition)

    2 How to remember biological concepts more easily?

    a By drawing flow charts through logical deductions

    Start with what the question asks for, ask yourself what comes next. Answer the question you

    ask. Base on your answer, ask yourself what comes next again. Repeat and you will get the

    answer for questions requiring detailed explanation.

    Example: What happens to the cells when a piece of onion epidermis is immersed in a

    hypertonic solution?

    QQ What is a hypertonic solution? AA A solution with water potential lower

    than that of the cytoplasm.

    QQ What happens when they differ in

    water potential?

    AA Water moves out of the cells through

    the differentially permeable membrane

    by osmosis.

    QQ What happens to the cells when

    they lose water?

    AA The cells shrink.

    QQ What happens to the cell membrane

    when the cells shrink?

    AA The cell membrane pulls away from

    the cell wall, i.e. plasmolysis occurs.

    b By using mnemonics ()

    It would be easier to remember some facts or concepts if you can make associations between

    different words. For example:

    c By using cut-off method ()

    Some biological processes are mirror images. For example:

    inhalation and exhalation

    accommodation of the eye to near and distant objects

    constriction and dilation of the pupil

    In each pair, you can focus on only one of the processes. The other process is simply the other way

    round.

  • New Senior Secondary Mastering Biology 19 Oxford University Press 2014 (Second Edition)

    PPaarrtt IIII

    SScchhooooll--bbaasseedd

    aasssseessssmmeenntt ((SSBBAA))

  • New Senior Secondary Mastering Biology 20 Oxford University Press 2014 (Second Edition)

    The SBA of biology covers the assessment of students performances in practical related tasks

    throughout the S5 and S6 school years. The tasks include laboratory work and fieldwork.

    Students will be assessed in two ability areas (A and B) by their teachers.

    Ability area A Ability area B

    Focus on practical skills planning and reporting of

    scientific investigation

    Assessment

    ability

    organizing and performing practical work, including the use of suitable

    apparatus and equipment, and the

    appropriate skills in carrying out the

    work

    making accurate observations and measurements

    identifying the problem to be

    investigated and formulating a

    hypothesis, where applicable

    devising a plan of investigation

    according to the problem identified

    recording and presenting results in an

    appropriate form

    interpreting and discussing results, and

    drawing appropriate conclusions

    The table below summarizes the percentage weighting and the minimum number of assessments

    required in S5 and S6 for the different areas of the SBA:

    Subject Biology Combined Science (Biology)

    Ability area A B A B

    Weighting 8% 12% 4% 6%

    Minimum number of assessment

    S5 1 1 1 1

    S6 1 1

    Details of the SBA can be found at the HKEAAs website.

    http://www.hkeaa.edu.hk/en/sba/sub_info_sba/ dse_subject.html?5

    99 AAbboouutt SSBBAA

  • New Senior Secondary Mastering Biology 21 Oxford University Press 2014 (Second Edition)

    A Making a temporary mount

    Making a temporary mount is the first step to prepare a specimen for microscopic examination.

    Steps of making a temporary mount are shown below:

    1 Place the specimen in the middle of a

    slide.

    2 Add 1 to 2 drops of water or stain to the

    specimen.

    3 Let the edge of a cover slip touch the

    mounting medium.

    4 Use a pair of forceps to lower the cover

    slip slowly and cover the specimen.

    5 Soak up excess mounting medium with

    tissue paper.

    6 The slide is ready for microscopic

    examination.

    1100 BBaassiicc eexxppeerriimmeennttaall tteecchhnniiqquueess

  • New Senior Secondary Mastering Biology 22 Oxford University Press 2014 (Second Edition)

    B Free-hand sectioning

    Free-hand sectioning () is the simplest method to prepare a thin section for a temporary

    mount. Steps of free-hand sectioning are shown below:

    1 Moisten both the razor blade and the

    specimen.

    2 Hold the specimen as shown below. Rest

    the razor blade on the tip of the forefinger.

    Adjust the position of the forefinger to

    control the thickness of the section.

    3 Cut the specimen by sliding the razor

    blade rapidly and smoothly towards

    yourself. Repeat the steps to prepare a

    number of sections.

    4 Put all the sections into a Petri dish of

    water. Use a paintbrush to select the

    thinnest section for examination.

  • New Senior Secondary Mastering Biology 23 Oxford University Press 2014 (Second Edition)

    C Microscopic examination

    1 Place the microscope on the bench with

    good illumination. Rotate the nosepiece

    and select the low-power objective.

    2 Look through the eyepiece. Adjust the

    angle of the mirror to reflect light towards

    the centre of the stage.

    3 Adjust the condenser and the

    diaphragm until the light is sufficient

    and even.

    4 Clip a prepared slide onto the stage. Watch

    the stage from the side and lower the body

    tube by turning the coarse adjustment knob

    until the objective nearly touches the slide.

    5 Look through the eyepiece again.

    Slowly raise the body tube by turning

    the coarse adjustment knob until the

    image of the specimen becomes clear.

    6 Turn the fine adjustment knob until the

    image is in focus.

    Skip this step if the

    microscope has a

    built-in light source.

  • New Senior Secondary Mastering Biology 24 Oxford University Press 2014 (Second Edition)

    D Using a pipette

    1 Rinse the pipette with distilled water

    followed by the liquid to be transferred.

    2 Insert the pipette filler into the pipette.

    Squeeze the pipette filler. Use another

    hand to hold the pipette.

    3 Insert the pipette into the liquid to be

    transferred. Release the filler slowly

    until the pipette is filled to about 2 cm

    above the graduation mark.

    4 Remove the pipette filler. Cover the mouth

    of the pipette by your forefinger.

    5 Release your finger to control the flow

    until the meniscus of the liquid sits on

    the graduation mark.

    6 Transfer the liquid to another container.

    Release the liquid. Touch the pipette tip

    against the side of the container to release

    the last drop of liquid.

    conical flask

    liquid transferred

  • New Senior Secondary Mastering Biology 25 Oxford University Press 2014 (Second Edition)

    When you observe something about the natural world that you do not understand, you may carry out

    investigations and try to find out the answer. How to carry out a scientific investigation is discussed

    in detail in the textbook (Bk 1A, Ch 1, p. 711). After performing the investigation, you should

    write a report to share what you have done and what you have discovered with other people. Below

    is an example to show how to write an investigation report.

    1111 SSkkiillllss iinn wwrriittiinngg iinnvveessttiiggaattiioonn rreeppoorrttss

    IInnvveessttiiggaattiioonn

    Peter bought some bread. He put it on a table but forgot to eat it. After a week, he

    found some bread mould growing on the bread surface. He wondered if light affected the

    growth of bread mould on bread. He carried out an investigation of it with the following

    set-ups.

    After a week, Peter put a transparency with a grid on the bread and counted the number of

    squares with bread mould in both set-ups.

    In set-up A, the number

    of squares with bread

    mould is 15.

    In set-up B, the number

    of squares with bread

    mould is 10.

  • New Senior Secondary Mastering Biology 26 Oxford University Press 2014 (Second Edition)

    An investigation report usually includes the following:

    1 Date

    2 Aim

    It gives the aim of the investigation.

    Example: To find out whether light affects the growth of bread mould on bread.

    3 Introduction

    a Problem to investigate

    Example: Does light affect the growth of bread mould on bread?

    b Hypothesis

    This part states the hypothesis of the investigation and the prediction based on the design of the

    investigation. It should be noted that hypotheses are NOT needed in investigations which do NOT

    require the explanation of observations (like Peters investigation).

    c Principle behind the design of the investigation

    This part includes:

    a brief description of the rationale of the design

    identification of the independent and dependent variables, and ways of manipulating and

    measuring the variables

    the controlled variables

    Example:

    Independent variable Dependent variable Controlled variables

    Variable Presence of light Number of squares with

    bread mould on the

    bread

    Kind of bread, air

    temperature, humidity,

    etc.

    Ways of

    manipulating and

    measuring the

    variable

    Keep a slice of bread in

    a light-proof box and

    keep another slice of

    bread in a transparent

    box

    Put a transparency with

    a grid on the bread and

    count the number of

    squares with bread

    mould

    the critical assumptions made, if any

    Example: The two slices of bread in the two set-ups are identical.

  • New Senior Secondary Mastering Biology 27 Oxford University Press 2014 (Second Edition)

    4 Procedure

    This is an account of what you have done during the investigation. It should be concise, precise and

    presented logically.

    5 Results

    There are different ways to present the results, e.g. drawing tables, plotting graphs, drawing

    biological diagrams, etc.

    a Drawing tables

    In a table, the data of the independent and dependent variables should be clearly put side by

    side for easy comparison.

    Example:

    b Plotting graphs (Refer to p. 13 for the skills in plotting graphs.)

    c Drawing biological diagrams (Refer to p. 14 for the skills in drawing biological diagrams.)

    Conditions Number of squares with bread mould

    Without light 15

    With light 10

    Points to note when writing Procedure

    1) Write in reported speech, i.e. past passive voice.

    2) Number the steps, rather than describing all the steps in a single paragraph.

    3) State the quantities of the apparatus and the materials used.

    4) Use diagrams or tables whenever appropriate.

    5) State any precautions to be taken.

    6) Do not repeat the details of a step when the step is just repeated with

    different kinds / amounts of materials.

  • New Senior Secondary Mastering Biology 28 Oxford University Press 2014 (Second Edition)

    6 Discussion

    In this part, you should evaluate how valid the investigation is and recognize trends and patterns in

    the data.

    7 Conclusion

    This part includes the conclusion drawn from the findings with regards to the aim of the

    investigation.

    Example: Light slows down the growth of bread mould on bread.

    It is not necessary to give further explanations, which are not tested by the experiment.

    Points to note when writing Discussion

    1) State the limitations of the experimental design.

    2) Suggest sources of error in the investigation and their influence on the

    accuracy of the results obtained.

    3) Consider any unusual observations and assess their significance.

    4) Give possible explanations for unexpected results.

    5) Suggest improvement for further work.

    Next time I should repeat the

    experiment with more bread to

    increase the reliability of the

    results.

  • New Senior Secondary Mastering Biology 29 Oxford University Press 2014 (Second Edition)

    Below is the investigation report written by Peter. There are many mistakes in the report. Can you

    point them out?

    Name: Peter Chan Class: 5A Class no.: 3

    Date of investigation: 1292014

    Aim

    To study the growth of bread mould on bread

    under different conditions.

    Introduction

    (a) Problem to investigate

    Does light affect the growth of bread mould on

    bread?

    (b) Hypothesis

    Bread mould does not grow in the presence of light.

    (c) Principle behind the design of the investigation

    Independent variable: Number of bread mould

    Dependent variable: Light

    Procedure

    I put a slice of bread into two different boxes. One

    of the boxes is a light-proof box and the other is a

    transparent box. I put the set-ups under light.

    After some time, I recorded the growth of bread

    mould on the two slices of bread.

    Only the effect of light

    was studied in the

    investigation.

    Peters investigation

    does not need an

    explanation of

    observation. Therefore

    a hypothesis is not

    required in this case.

    1) Steps should be

    numbered.

    2) Reported speech

    should be used.

    3) The duration of the

    experiment should

    be clearly stated.

    1) Variables are

    wrongly stated.

    2) Controlled variables

    and assumptions

    should be

    mentioned.

  • New Senior Secondary Mastering Biology 30 Oxford University Press 2014 (Second Edition)

    without light with light

    num

    ber

    of s

    quare

    s w

    ith

    brea

    d m

    ould

    on t

    he

    brea

    d

    5

    10

    15

    0

    Results

    The following are the results of the experiment:

    Conditions Number of squares with bread

    mould on the bread

    Without light 15 cm

    With light 10 cm

    1) The units are

    wrong.

    2) The units, if any,

    should be put in the

    heading of the

    column, not in the

    boxes.

    It is not necessary to

    draw a graph for this

    investigation as the

    graph does not help

    analyse the data.

  • New Senior Secondary Mastering Biology 31 Oxford University Press 2014 (Second Edition)

    Discussion

    Much less bread mould grew on the slice of bread

    under light, showing that light can inhibit the

    growth of bread mould. This is because the

    ultraviolet light in the light can kill the spores of the

    bread mould.

    As the difference in the number of squares with

    bread mould in the two set-ups is large, the results

    are accurate and reliable.

    Conclusion

    Light can prevent the growth of fungi because the

    ultraviolet light can kill the spores of the fungi.

    1) the ultraviolet light

    in the light can kill

    the spores of the

    bread mould is a

    new hypothesis, not

    a fact.

    2) Further tests should

    be suggested to test

    this hypothesis, e.g.

    block the ultraviolet

    light in the light and

    carry out a similar

    investigation again. 1) The reason for the judgment of the accuracy and

    reliability of the experimental results is wrong.

    2) As only one measurement is taken, we cannot say

    that the results are reliable. Thus, this report lacks a

    critical analysis of the data obtained.

    The conclusion is over

    generalized (applying

    the finding to all

    fungi) and contains

    irrelevant explanation

    (the role of ultraviolet

    light is a new

    hypothesis).