histology lab 1 introduction and epithelium jun zhou (周俊), ph.d & m.d school of...
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Your final score is composed of four parts 1) Attendance and picture drawing:10% 2) Quiz: 15% (each quiz 5%) 3) Final lab test: 25% 4) Final written examination: 50% How to pass this course: Final score>60 and Final written exam>50TRANSCRIPT
Histology Lab 1
Introduction and Epithelium
Jun Zhou(周俊) , Ph.D & M.DSchool of Medicine,Zhejiang University
Note:Please come lab on time.Find your seat according your lab number.
Your final score is composed of four parts 1) Attendance and picture drawing:10% 2) Quiz: 15% (each quiz 5%) 3) Final lab test: 25% 4) Final written examination: 50%
How to pass this course: Final score>60 and Final written exam>50
Lab facilities: 1 Light electron microscope with computer 1 case of slides 1 atlas http://m-learning.zju.edu.cn 1.Students share these facilities2.Do not take them out the laboratory with you.3.Put them where they belong to when lab is over.4.Do not left any rubbish after you.5.No food in the lab.6.Lecture and lab slides online: m-learning.zju.edu.cn 2015 Histology and Embryology
Contents of Lab 1:1. Introduction
2. Epithelium
1)Simple squamous epi (small intestine,stomach)
2)Simple cuboidal epi.(thyroid)
3)Simple columnar epi.(small intestine)(drawing)
4)Pseudostratified ciliated columnar epi.(trachea)
5)Stratified squamous epi.(esophagus)
6) Transitional epi.(bladder)
3.Lab report (content of lab , drawing)
Tissue ProcessingTissue Processing
1.Fixation: 10% Formalin
2.Dehydration:graded concentrated ethyl alcohol
3.Clearing:xylene,infiltrate with paraffin solvent.
Loss of fat due to ‘clearing’
Tissue ProcessingTissue Processing
4.Embedding4.Embedding
5.Sectioning5.Sectioning
Tissue ProcessingTissue Processing6.Staining6.Staining
Common Stains: Hematoxylin & EosinCommon Stains: Hematoxylin & Eosin
Ross, 5th ed, fig 1.1
Common StainsCommon Stains
Wright’s stain
Hematoxylin and eosin ("H&E")
Relative Sizes in HistologyRelative Sizes in Histology
Standard Units of MeasureStandard Units of Measure
micrometer: 10-6 meter
nanometer: 10-9 meter
Relative Sizes in HistologyRelative Sizes in Histology
Standard Units of MeasureStandard Units of Measure
Changes in apparent shape, Changes in apparent shape, due to orientation of sectionsdue to orientation of sections
Ross, 5th ed, fig 1.11
Changes in apparent shape, Changes in apparent shape, due to orientation of sectionsdue to orientation of sections
Netter/Ciba
Junqueira fig 1-30
Epithelium
•No blood vessles, nourishment is by diffusion•Epithelium is innervated•polarization
Trachea
Epithelium
Classification of Epithelia•Number of cells1. Simple
2. Pseudostratified
3. Stratified
•Shape of surface cells1. Squamous
2. Cuboidal
3. Columnar
4. Transitional
Stomach (NO 25): mesothelium HE ×400
Simple squamous epi.
Small intestine (NO 1): endothelium HE × 400
Thyroid (NO 35): HE × 100
Thyroid: HE × 400
Simple cuboidal epi.
Small intestine (NO 1): villus HE × 400 (Drawing )
Simple columnar epi., Goblet cells, striated border (microvilli)
Trachea Pseudostratified ciliated columnar epi
Trachea: HE × 400
Pseudostratified ciliated columnar epi.
Esophagus (No.2)
Stratified squamous epi.
Esophagus: HE × 100
Transitional epitheliumTransitional epithelium
distended contractedBladderBladder
Bladder (NO33): HE × 40
Bladder: HE × 100 Transitional epi.
Bladder: HE × 400
Transitional epi.
The end!