High resolution clinical phenotype:

• Demographics• Socio-Economic status• Birth History• Social History• Family History• Pre-kidney disease H.• Kidney disease H.• Medication H.

• Clinical Symptoms• Physical Exam• Quality of Live • Patient recorded

outcomes (PROMIS)• Adverse Events• Histopathology

(local and digital repository)

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4-6 monthly visits,473 parameters captured in 1053 data fields

NEPTUNE Virtual Microscopy Archive of Nephrotic Syndrome

Glass slides are scanned usingAperio ScanScope OS

Data analysis

Kidney bx slide

Scoring process on annotated images

Annotator

Scoring process on annotated images

Reader #1 Reader #2

L. Barisoni, S.Hewitt, J. Kopp

Comprehensive Sample Procurement

• DNA:– blood, kidney, urine

• RNA: – Blood, kidney, urine

• Serum• Plasma

– EDTA, NaHep, NaCitrate

• Urine– Spot / 24h– Native, NaAz, Protease In.– Urine sediment

Funded:• Targeted Sequencing• GWAS• Nephron-segment specific

Transcriptome• Digital Path incl. glomerular

Morphometry

Options to capture genome wide profiles along genotype – phenotype continuum

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