NANOHETER Assessing the heteroaggregation of manufactured nanoparticles

with naturally occurring colloids in a typical surface water

• Aix-Marseille Université, CNRS, CEREGE, Aix en Provence, France • ETH Zurich, Institute for Chemical and Bioengineering, Zurich, Swiss • BRGM, Orléans, France • University of Wyoming, Laramie, WY, USA

ERA-NET SIINN NANOHETER program 2013-2016

ERA-NET SIINN funded project, 1st Call 2012 http://nanoheter.cerege.fr

Project Duration: 36 months Started on April, 1st, 2013 Total Costs: 1,214,054€ ERA-NET SIINN funding: 526,352€

Objectives of NANOHETER project: As part of ENP exposure assessment,

Study the physical fate of ENPs in surface water under relevant conditions:

low dose, complexe environment, (ENM lifecycle)

NANOHETER Partners

• CEREGE – CNRS, Aix-Marseille University (Aix en Provence, France), funded by ANR J. Labille (coord.), D. Slomberg (postdoc funded), O. radakovitch, J.-Y. Bottero (iCEINT coord.),... Long experience in environmental physical-chemistry, and ENP exposure http://www.cerege.fr, http://i-ceint.org

• ETH, Zürich (Swiss Federal Institute of Technology), funded by FOPH

M. Scheringer, N. Sani-Kast (PhD thesis funded), A. Praetorius A technical university run by the Swiss Federal Government, long tradition in chemistry and environmental sciences; http://www.ethz.ch Safety and Environmental Technology Group, Department of Chemical and Bioengineering at ETH: Mandate by the Swiss chemical industry to investigate health and safety risks of chemical processes and products; http://www.sust-chem.ethz.ch

• BRGM (Orléans, France), funded by ANR P. Ollivier, S. Ilina (postdoc funded), N. Baran French national institute for research on earth and environment sciences Divisions “Water, Environment and Ecotechnology”, and “Monitoring and Analysis” http://brgm.fr

• University of Wyoming, Laramie, USA, self funded partner (PUF Grant 2011-2014)

Jonathan Brant Department of Civil and Architectural Engineering

WP1 - Naturalist approach Case study: Rhone River, France

9 sampling sites Water characterisation

(SPM, NOM, ions, chemical comp., pesticides)

Slomberg et al., submitted

• ENPs heteroaggregate rapidly with SPM, even at low dose.

• This alters the fate of both ENP and SPM • Need to understand the driving mechanisms

ENP injection in river water at t0

100 µg/L ENP

10 µg/L ENP

Size evolution of SPM when spiking ENP laser diffraction nano-TiO2 5-30 nm

WP 2-3 Mechanistic approach

Homoaggregation of nano-TiO2 vs. NaCl,

pH = 5; TiO2 = 10 mg/L measured by DLS

Clay/nanoparticle heteroaggregation vs. ENP concentration (0.1−4 mg/L),

clay concentration = 100 mg/L, NaCl (10-1 M), pH = 5,

measured by laser diffraction

Labille et al., ES&T, 2015

WP 4: Modelling the Fate of ENPs in Natural Surface Water

• Model integrates results of experimental work into a quantitative ENP mass balance for water and sediment

• Shows interplay of

– transport with moving water – heteroaggregation and subsequent deposition with SPM – resuspension

• Facilitates uncertainty analysis; analysis of scenarios, including other European river systems with different conditions

• Model described by Sani-Kast et al. (2015)1

1: http://dx.doi.org/10.1016/j.scitotenv.2014.12.025

WP4 - Model Description and

Application

Concentration profile of ENPs simulated in the Rhone River, water column vs. sediment for a ponctual source at d=0

Dissemination: Publications

1. Praetorius A., Labille J., Scheringer M., Thill A., Hungerbuehler K., Bottero J.Y., Heteroaggregation of titanium dioxide nanoparticles with model natural colloids under environmentally relevant conditions, Environ. Sci. Technol. (2014) 48, 10690-10698

2. Sani-Kast N. Scheringer M., Slomberg D., Labille J. Hungerbühler K. Addressing the Complexity of Water Chemistry in Environmental Fate Modeling for Enginered Nanoparticles, Journal of the Total Environment, (2015) in press, doi:10.1016/j.scitotenv.2014.12.025

3. Labille J., Harns C., Bottero J.-Y., Brant J., Heteroaggregation of titanium dioxide nanoparticles with natural clay colloids. Environ. Sci. Technol (2015) in press. DOI: 10.1021/acs.est.5b00357

4. Slomberg D, Patrick Ollivier, Olivier Radakovitch, Nicole Baran, Nicole Sani-Kast, Hélène Miche, Daniel Borschneck, Olivier Grauby, Auguste Bruchet, Martin Scheringer, and Jérôme Labille. Characterization of suspended particulate and natural organic matter in the Rhône River: Insights into selecting surface water analogues. submitted to Environmental Chemistry

Dissemination: Communications in international conferences last year 1. International congress on safety of engineered nanoparticles and nanotechnology (SENN 2015), 12-15.04.2015, Helsinki, Finland - Evaluation of

manufactured nanoparticle heteroaggregation with suspended particulate and dissolved organic matter in natural surface waters. Slomberg, D.; et al. (poster)

2. International congress on safety of engineered nanoparticles and nanotechnology (SENN 2015), 12-15.04.2015, Helsinki, Finland - Addressing the Complexity of Water Chemistry in Environmental Fate Modeling for Engineered Nanoparticles. (poster)

1. Sustainable nanotechnology organization (SNO) (9-11.03.2015, Venice, Italy) - Addressing the Complexity of Water Chemistry in Environmental Fate

Modeling for Engineered Nanoparticles. (poster)

2. Sustainable nanotechnology organization (SNO) (9-11.03.2015, Venice, Italy) - Evaluation of titanium dioxide nanoparticle fate and heteroaggregation in natural surface waters. Slomberg, D.; Labille, J.; et al. - Oral presentation

3. Second Marine NanoEcosafety Workshop, Palermo, Italy, 17-18 November 2014; Assessing the behavior and fate of manufactured nanoparticles in seawater. Case study for nano-TiO2 used in sunscreen. J. Labille (keynote)

1. Fluid Particle Separation FPS 2014, Lyon France, October 15-17, 2014 - Nanoparticle Transport and Removal Mechanisms in Membrane Filtration, Brant

J. A., Ruff L., Tan G., Labille J. – oral presentation 1. 24th SETAC Europe 2014, Basel, Swiss, 11-15 May 2014 - Fate and heteroaggregation of titanium dioxide nanoparticles in natural surface waters ;

Danielle L. Slomberg, Patrick Ollivier, Jérôme Labille - oral presentation 1. 24th SETAC Europe 2014, Basel, Swiss, 11-15 May 2014 – Environmental fate models for engineered nanoparticles – Simulating realistic condition in a

complex natural river system ; N. Sani-Kast, A. Praetorius, et al. - oral presentation 1. 24th SETAC Europe 2014, Basel, Swiss, 11-15 May 2014 - Environmental fate models for engineered nanoparticles – simulating realistic conditions in a

complex natural river system (poster)

2. 2014 CEINT Internal Meeting, Durham (NC), USA 22-23 May 2014 - Fate and heteroaggregation of titanium dioxide nanoparticles in natural surface waters. Slomberg, D.; Labille, J.; Ollivier, P. - oral presentation

3. European Geoscience Union General Assembly (EGU) 2014, Vienna, Austria 27 Apr-2 May 2014 - Assessing the heteroaggregation of manufactured nanoparticles with geogenic colloids in surface water ; Danielle Slomberg, Jérôme Labille, et al. - oral presentation

Communications coming soon 1. 10th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials, ICEENN, Vienna, Austria, 6-10/09/15

oral presentation by Slomberg et al.

2. SETAC North America 36th annual meeting, Salt Lake City, USA – 1-5 November 2015 - Dedicated session chaired Assessing the Environmental Fate and Exposure of Engineered Nanoparticles Under Relevant Conditions – oral presentation by Slomberg et al.

Web site here: http://nanoheter.cerege.fr