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he_supp. fig.1

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He_Supp. Fig.1 ATG (1) TG A (3519) rdm7 ( nrpd2a) AT1G63020 AT2G40030 B AT3G23780 C A AT4G20910 AT5G63110 E F AT2G27040 D G AT2G16390 rdm8 ( ago4 ) 6 bpdeletion ATG (1) TAA (2775) ATG (1) TAA (1416) rdm10 (hda6) 13 bpdeletion rdm5 ( nrpd1a ) ATG (1) TGA (4362) ATG (1) TAA (5931) rdm6-2 ( nrpd1b) rdm6-1 ( nrpd1b ) 1377 bpdeletion TGA (2829) rdm9 ( hen1) ATG (1) rdm11 (drd1) 35 bpdeletion ATG (1) TAA (2667)

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He_Supp. Fig.1. (. ). rdm5. nrpd1a. ATG (1). TGA (4362). (. rdm6. -. 2. nrpd1b). (. ). rdm6. -. 1. nrpd1b. 1377. bp. deletion. ATG (1). TAA (5931). (. ). rdm8. ago4. 6. bp. deletion. ATG (1). TAA (2775). (. ). rdm9. hen1. TGA (2829). ATG (1). rdm10 (hda6). - PowerPoint PPT Presentation

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Page 1: He_Supp. Fig.1

He_Supp. Fig.1

ATG (1) TGA (3519)

rdm7 (nrpd2a)

AT1G63020

AT2G40030

B

AT3G23780

C

A

AT4G20910

AT5G63110

E

F

AT2G27040

D

GAT2G16390

rdm8 (ago4)6 bp deletion

ATG (1) TAA (2775)

ATG (1) TAA (1416)

rdm10 (hda6)13 bp deletion

rdm5 (nrpd1a)

ATG (1) TGA (4362)

ATG (1) TAA (5931)

rdm6-2 (nrpd1b)

rdm6-1 (nrpd1b)1377 bp deletion

TGA (2829)

rdm9 (hen1 )

ATG (1)

rdm11 (drd1)35 bp deletion

ATG (1) TAA (2667)

Page 2: He_Supp. Fig.1

RD29A

NPTII

COR15A

TUB8

4 °C

WT23

°C

4 °C

23 °

C

23 °

C

4 °C

ros1 ros1rdm6-1

rRNA

RD29A

NPTII

COR15A

LUC

4 °C

WT

23 °

C

4 °C

23 °

C

23 °

C

4 °C

ros1 ros1rdm8

TUB8

rRNA

RD29A

NPTII

COR15A

LUC

TUB8

4 °C

WT

23 °

C

4 °C

23 °

C

23 °

C

4 °C

ros1 ros1rdm10

rRNA

A nrpd1b B ago4

C hda6

RD29A

NPTII

COR15A

LUC

TUB8

4 °C

WT

23 °

C

4 °C

23 °

C

23 °

C

4 °C

ros1 ros1rdm11

rRNA

drd1D

He_Supp. Fig.2

Page 3: He_Supp. Fig.1

B

WT

ros1

ros1

nrpd

1b

ros1

hda6

ros1

ago4

ros1

drd1

5S rDNA repeat Southern

HpaII MspI HaeIII

siRNA1003

microRNA173

U6 snRNA

AtMU1

WT ros1

ros1

hda6

WT

ros1 ros1

ago4

WT

ros1 ros1

drd1

AtGP1

TUB8

WT

ros1

ros1

nrpd

1bW

T

ros1

ros1

hda6

WT

ros1

ros1

ago4

WT

ros1

ros1

drd1

AtSN1 HaeIII

AtSN1 No Digest

A

CD

WT

ros1

ros1

nrpd

1b

ros1

hda6

ros1

ago4

ros1

drd1

WT

ros1

ros1

nrpd

1b

ros1

hda6

ros1

ago4

ros1

drd1

WT

ros1

ros1

nrpd

1b

ros1

hda6

ros1

ago4

ros1

drd1

He_Supp. Fig.3

Page 4: He_Supp. Fig.1

Col-0nr

pd4-

2

Col-0nr

pd4-

2

nrpd

1a

nrpd

1b

nrpd

1b

nrpd

1a

Col-0nr

pd4-

2

nrpd

1b

nrpd

1a

MspI HaeIIIHpaII

180 bp CEN BA

Cluster4 siRNA

Col

-0

nrpd

1a

nrpd

4-2

nrpd

1b

AtSN1 siRNA

siRNA1003

tRNA and 5S rRNA

AtGP1 siRNA

AtMU1 siRNA

siRNA02

siRNA255

microRNA171

He_Supp. Fig.4

Page 5: He_Supp. Fig.1

SD-Trp-Leu-His+50 mM 3AT SD-Trp-Leu

A

AD BD

NRPD4 AGO4

NRPD4 Vector

AGO4 NRPD4

Vector NRPD4

B

C

He_Supp. Fig.5


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