HC STUDENT - FACULTY MIXER WITH COLLEGE OF SCIENCE

Kevin Brown – Complex Systems

• Mathematical modeling of biochemical networks

• Network structure/function relationships

• Biological, Neurological, and Chemical Informatics

• Semantic and Phonological Networks

• Synchronization in Networks of Coupled Oscillators

Mental LexiconSystems Biology

High throughput screening of CPA

toxicity

Informatics/Machine Learning

Cognitive Processing

Pharmaceutical Sciences and CBEE kevin.brown@oregonstate.edu

“Hard” Protein ChemistryPhil McFadden lab in the Department of Biochemistry and Biophysics

Hydroxyapatite

1. QUESTIONWhile it is known that tooth enamel is strengthened by fluoride, what is the effect of the background water?

2. EXPERIMENTAL SYSTEM Fluoride exposure testing

3. RESULTSpH 6 is optimal, but aside from that, ten different water sources showed remarkably similar fluoridation potentials.

4. Karissa Renyer D.M.D. Candidate, Class of 2024OHSU School of Dentistry

plus Fluoride

1. The amazing thing that cells like neurons or muscles do is…

they fire!

Electrophysiology: How do Living Excitable Cells Behave?

2. Some cells fire a lot; others don’t. To find out, you recordfrom them

3. When and how do cells fire? Towhom & how strongly do they connect? Do drugs, disease, or mutations change this?

Come study how the living brain works!

Kenton Hokansonhokansok@oregonstate.eduElectrophysiology Facility

DazlEgalitarian

MAP-4

Gephyrin

IC

Kibra

LVPhosphoprotein

MyosinVa

nNOSNRF1Nup159p53BP

Pak1

PTH mRNA

Rabies Phosphoprotein

RasGRP3

Swa

Syntaphilin

TRPS1 Bim

Chica

ASCIZ

NeurotransmissionCell Death

Development

Cell DivisionViral Infection

Tumor Suppression Cell Signaling

LC8 Is a Hub Protein

Barbar (2008) Biochemistry

HPIVEboVP35(Joaquin, Brianna)

Dynein assembly &regulation

(Kayla, Cat)

Panx, transcriptional silencing of transposonsCRUK Cambridge Institute(Kaya)

Kank, anchor adhesion protein at the cell cortexUtricht University(Aidan, Seth, Yu Zhen)

Sabbatical PUIN. Loening, NSF funding, Murdock funding for 600 MHz

Barbar lab- Internationally diverse labTake risks, insist, persist and resist

Prx—Karplus, OSUCrystallin- Lampi, OHSUKinesin, Qiu, OSU

Liman Zhang- OHSUJonathan Pruneda, OHSUCarsten Schultz, OHSU

Panx, UKKank, Utrecht

Multi-PI R01 proposalComputation--ZuckermanNSEM—ReichowNative mass spec– PrellAUC—Reardon

Under review

In cell NMR, ReardonFluorescence, Howe

Younger generation publicSocial media: Tiktok, twitter,

Undergraduate researchURSA ENGAGE (3)NSF Supplements (2)NSF PUI Supplements

SARS-CoV2 NEAGER grant, “Structure and Assembly of SARS-CoV2 nucleocapsid”--Funded

Multi-PI R01 proposal as an Emergency Award: Rapid Investigation of SARS-CoV-2 and COVID-19Under review

Barbar Lab - A Year in Review Publications—9 since Sep 2019

Outreach

Extra

Elisar Barbar chaired an NIH study section, Special Emphasis panel for F04B proposals in Biological Chemistry and Macromolecular Biophysics in July.

• Mostofian, B., McFarland, R., Estelle, A., Howe, Barbar, E., Reichow, SL., Zuckerman, DM (2020) Automated and statistically corrected identification of flexible multivalent IDP-bound assemblies in electron micrographs BioRxiv https://doi.org/10.1101/2020.06.16.154096

• Loening NM and Barbar, E. (2020) Structural characterization of the coiled coil domain of the LC8 binding partner Swallow. Protein Structure Report, in press.

Heather Masson- Forsythe’s TikTok Videos https://www.tiktok.com/@heycurlytop?lang=en

Elisar Barbar served on an NIH study section panel for RM1 proposals in May.

Nathan Jespersen defended his thesis! Elisar Barbar became the head of the departmentKayla Jara : Herbert F. Frolander Award for Outstanding Graduate Teaching AssistantsHeather Masson-Forsythe: HP InternSteller Customer Impact Award, runner-up

Elisar Barbar served and chaired an NIH study section in March.

• Jespersen N, Barbar E.J. (2020). Emerging Features of Linear Motif-Binding Hub Proteins. Trends in Biochemical Sciences Feb 20. https://doi.org/10.1016/j.tibs.2020.01.004

• Reardon P.N*, Jara K.A*, Rolland A.D, Smith D.A, Hoang H.T.M, Prell J.S, and Barbar E.J. (2020) The dynein light chain 8 (LC8) binds "in-register" to multivalent intrinsically disordered partners. J Biol Chem. Mar 5. pii: jbc.RA119.011653. doi: 10.1074/jbc.RA119.011653.

Heather Masson-Forsythe MC’d Grad Inspire event

• Jespersen NE, Leyrat C, Gérard FC, Bourhis JM, Blondel D, Jamin M, Barbar E. (2019) The LC8-RavP ensemble Structure Evinces A Role for LC8 in Regulating Lyssavirus Polymerase Functionality. J Mol Biol. Oct 18. pii: S0022-2836(19)30607-2. doi:10.1016/j.jmb.2019.10.011.

• Jespersen N, Estelle A, Waugh N, Davey NE, Blikstad C, Ammon YC, Akhmanova A, Ivarsson Y, Hendrix DA, Barbar E. (2019) Systematic identification of recognition motifs for the hub protein LC8. Life Sci Alliance. Jul 2;2(4). pii: e201900366. doi: 10.26508/lsa.201900366.

Heather married her partner, Margaux, in July on Mt. Hood

Heather Masson-Forsythe is a DJ for the Radio Show ‘Inspiration Dissemination’ on campus,she was also interviewed this year for her work in the lab

Kayla Jara is an OMSI Science Communication Fellow

Heather Masson-Forsythe continues her internship at HP

We welcomed Yu Zhen to the lab as our new lab manager

• Forsythe, H. M., Vetter, C. J., Jara, K. A., Reardon, P. N., David, L. L., Barbar, E. J., & Lampi, K. J. (2019). Altered protein dynamics and increased aggregation of human γS-Crystallin due to Cataract-Associated deamidations. Biochemistry, 58(40), 4112-4124.

Heather Masson-Forsythe interviewed on Chemistry Cayk Online Podcast

• Loening NM, Saravanan S, Jespersen NE, Jara K, Barbar E. Interplay of Disorder and Sequence Specificity in the Formation of Stable Dynein-Dynactin Complexes. Biophys J. 2020;119(5):950-965. doi:10.1016/j.bpj.2020.07.023

• Tseng, K-F, Mickolajczyk, K.J., Feng, Q., Howe, J., Barbar, E., Dawson, S.C., Hancock, W.O., and Qiu, W. (2020) The N-terminal Tail Dual-Regulates the Motility of Giardia Kinesin-14a. Current Biology S0960-9822(20)30942-8.

CollaborationsJim Prell’s group at UO Anna Akhmanova group (Utrecht)Niko Loening at Lewis & Clark Andy Karplus (OSU) Cancer Research UK (CRUK) Dave Hendrix (OSU)Dan Zuckerman (OHSU) Liman Zhang (OHSU)Steve Reichow group (PSU) Rick Cooley (OSU)Weihong Qiu (OSU) Blondel, Jamin, Longhi (virologists in France)

Awards & Achievements

Jesse Howe and Aidan Estelle managed a recruiting table at a Grad Recruiting event at the Biophysical Society meeting.

Microfluidic Analytical Systems and SensorsRemcho Research Group • remcho.science.oregonstate.eduVince Remcho, UHC Eminent Professor, Chemistry & Materials Science296 Linus Pauling Science Center • vince.remcho@oregonstate.edu

Microfluidic systems• for medical diagnostics• for chemical sensing & analysis• for synthesis/purification of nanomaterials

We innovate in the areas of:• materials and surface chemistry• fluidic design & polymer chip fabrication• Chemical and biochemical separations and analysisOur research results in new materials & methods that are inherently greener and more efficient than their conventional counterparts.

What we study:• Intertidal ecology• Sea star wasting disease impacts• Long-term data collection on the

Oregon coast (some projects for 25+ years!)

• Climate change biology• Invertebrate and algal physiology

Menge Lab Research Opportunities

Chantelle MacAdams (she/her)Technician / Volunteer coordinatoradamsch@oregonstate.edu

Role of Oxidants in Tumor Development and GrowthPeroxynitrite & Tyrosine

nitrationTumors of the nervous

system

Role of Oxidants in Tumor Development and GrowthPeroxynitrite & Tyrosine

nitrationTumors of the nervous

system

Proteins

DNA

Lipids

ONOO-

Nitrotyrosine

ONOO-

Tyrosine

NitrotyrosineTubulin

DAPI (nuclear staining)

Human U87 cells

Maca Franco, Ph.D.(She, her, hers)

Biochemistry and Biophysicsmaria.franco@oregonstate.edu

ALS 2103 / (541) 737-4997

Drugs that specifically target the nitrated form of the

protein• Surface Plasmon resonance

• In vitro functional assays• Drug screenings

Translational Research

Structural Biology

Protein gain-of-function How does nitration affects protein

structure to induce a gain-of-function?• Analytical ultracentrifugation

• Negative stain• Cryo-EM

Role of Oxidants in Tumor Development

and GrowthPeroxynitrite &

Tyrosine nitrationCell and Molecular BiologyRole of Nitration in tumor growth

• Relevant nitrated proteins• Cell metabolism

• Signaling pathways• Mechanism of action

• OMICSIn vitro and

in vivo models

13

• Xiang (Shawn) Zhang, Ph.D.

• Research Associate

• xiang.zhang@osucascades.edu

• WET Lab, 2600 NW College Way, PON 101, Bend, OR, 97703

• Research of interest:

– Additive manufacturing (both metal and plastic 3D printing)

– Heat transfer & compact heat exchanger

– Wastewater treatment & system energy saving

Cell Biology of Cnidarian-Algal SymbiosisVirginia Weis Lab –Integrative Biology (IB)

Exaiptasia pallida (Aiptasia)tropical sea anemone host

Breviolum minutumdinoflagellate symbiont

Itchung Cheung

Academic Programs Manager & Senior Instructor | Hatfield Marine Science Center | Department of Integrative Biology

http://hmsc.oregonstate.edu/academics

541-867-0380 phone Itchung.cheung@oregonstate.edu

Fighting infection with vitamin D and other natural compounds

Nanofiber bandages and sutures

Chen S, Ge L, Gombart AF, Shuler FD, Carlson MA, Reilly DA, Xie J. Nanofiber-based sutures induce endogenous antimicrobial peptide. Nanomedicine (Lond). 2017 Nov;12(21):2597-2609. doi: 10.2217/nnm-2017-0161. Epub 2017.

Adrian F. Gombart, Linus Pauling Institute, Department of Biochemistry & Biophysics

Jiang J, Zhang Y, Indra AK, Ganguli-Indra G, Le MN, Wang H, Hollins RR, Reilly DA, Carlson MA, Gallo RL, Gombart AF, Xie J. 1α,25-dihydroxyvitamin D₃-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression. Nanomedicine (Lond). 2018 Jun;13(12):1417-1432.PMCID: PMC6219435.

Lowry MB, Guo C, Zhang Y, et al. A mouse model for vitamin D-induced human cathelicidin antimicrobial peptide gene expression [published online ahead of print, 2019 Nov 26]. J Steroid Biochem Mol Biol. 2019;198:105552. doi:10.1016/j.jsbmb.2019.105552

Fighting fat with natural compounds

Zhang Y, Bobe G, Revel JS, Rodrigues R, Sharpton TJ, Fantacone ML, Raslan K, Miranda CL, Lowry MB, Blakemore PR, Morgun A, Shulzhenko N, Maier CS, Stevens JF, Gombart AF. Improvements in Metabolic Syndrome by Xanthohumol Derivatives are Linked to Altered Gut Microbiota and Bile Acid Metabolism. Mol Nutr Food Res. 2019 Nov 22;e1900789. PMID: 31755244.

Paraiso IL, Plagmann LS, Yang L, Zielke R, Gombart AF, Maier CS, Sikora AE, Blakemore PR, Stevens JF. Reductive Metabolism of Xanthohumol and 8-Prenylnaringenin by the Intestinal Bacterium Eubacterium ramulus. Mol Nutr Food Res. 2019 Jan;63(2):e1800923. PMCID: PMC6561348.

Questions?

Alex Michels and Joe Pardo