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Announcement of population data

Haplotype diversity in mitochondrial DNA hypervariable region

I, II and III in a Korean ethnic group from northeast China

YongJi Zhang a,b,*, QingSong Xu a, Hong Cui a, Yan Cui a, HaiYu Lin a,b,KiBeom Kim b, JungBin Lee b

a Department of Forensic Medicine, Yanbian University College of Medicine, 121 Juzi-jie, Yanji 133000, Jilin, PR Chinab Department of Forensic Medicine, Seoul National University College of Medicine, 28 Yeongon-dong,

Chongno-gu, Seoul 110-799, South Korea

Received 27 November 2004

Available online 5 March 2005

www.elsevier.com/locate/forsciint

Forensic Science International 151 (2005) 299–301

Abstract

Sequence polymorphism of the mitochondrial DNA (mtDNA) control region, hypervariable regions HVR I, II and III, from

55 unrelated Korean ethnic group individuals from northeast China (YanBian area) were determined by PCR amplification and

cycle sequencing.

# 2005 Elsevier Ireland Ltd. All rights reserved.

eywords: Mitochondrial DNA; Control region; Hypervariable region; Korean ethnic group

Population: Healthy unrelated Korean ethnic group

volunteers living in the northeastern part of China so called

Yan Bian area.

DNA extraction: Phenol–chloroform–isoamyl alcohol

extraction [1].

Amplification and sequencing of mtDNA: PCR ampli-

fication was performed using primers, L15751-H16439 for

HVR I and L048-H922 for HVR II and III [2], amplified

products were cleaned using QIAquick1 PCR Purification

Kit (QIAGEN, Germany), followed by sequencing employ-

ing BigDyeTM Terminator Cycle Sequencing Ready reaction

(PE Applied Biosystems, USA). Unincorporated dye termi-

nators were removed using the SephadexTM G-50 Fine

(Amersham Biosciences, Sweden), followed by electrophor-

esis in ABI prism (377) automatic sequencer (PE Applied

Biosystems, USA).

Quality control: According to Yao et al. [3].

* Corresponding author. Tel.: +86 433 266 0590; fax: +86 433 265 97

E-mail address: zhangyj@ybu.edu.cn (Y. Zhang).

379-0738/$ – see front matter # 2005 Elsevier Ireland Ltd. All rights

oi:10.1016/j.forsciint.2005.02.002

Analysis of data: Haplotype diversity (h) was calculated

according to Tajima [4], and random match probability ( p)

was calculated according to Stoneking et al. [5].

Result: See Table 1.

Other remarks: All the sequences were aligned with

the Anderson reference sequence [6]. In the HVR I, II and

III, within the 55 unrelated individuals were found 54

different haplotypes as determined by 97 variable posi-

tions. The position 16223, 16362, 073 and 263 variations

are similar with the South Korean and Japanese [7,8]. The

haplotype diversity (h) and random match probability ( p)

were 0.9993 and 1.9 � 10�2, respectively. Also in side

regions (15752–16023, 16366–16438, 049–072 and 620–

921) of HVR were found 12 variable positions; among

them the 750 (A!G) position variation was found for all

samples, and all haplotypes were unique when consider

HVR with side regions.

95.

reserved.

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0Table 1

Variable sites observed in the mtDNA HVR I, II, III and side regions

Sample HVR I

(16024–16365)

HVR II

(073–340)

HVR III

(341–619)

Side regions

(full number)

S1 184 223 311 362 073 263 309.1C 315.1C 489 750 827 856

S2 129 223 298 327 073 146 249D 263 309.1C 309.2C 315.1C 489 750

S3 223 261 073 150 260 309.1C 315.1C 750

S4 223 231 362 073 263 309.1c 315.1C 489 508 750

S5 129 223 362 073 152 263 309.1C 315.1C 489 750

S6 187 223 290 319 073 235 263 291T 315.1C 523D 524D 663 750

S7 187 223 290 319 073 235 263 309.1C 315.1C 523D 524D 663 750

S8 183C 189 223 362 073 146 150 252 263 309.1C 309.2C 315.1C 456 489 681 750

S9 168 189 217 249 325 073 200 257 263 315.1C 573.1C 573.2C 573.3C 16390 750

S10 223 257A 261 073 150 204 263 309.1C 315.1C 750

S11 223 257A 073 150 263 315.1C 750

S12 129 162 304 073 249D 263 309.1C 315.1C 523D 524D 16390 064 750

S13 209 223 262.1C 324 073 263 309.1C 309.2C 315.1C 489 523D 524D 750

S14 223 362 073 146 263 309.1C 309.2C 315.1C 489 750

S15 223 362 073 263 315.1C 489 750

S16 223 278 362 073 263 315.1C 489 573.1C 573.2C 573.3C 573.4C 750

S17 129 223 362 073 263 315.1C 489 750

S18 086 167 183C 188.1C 189 223 319 362 073 150 263 309.1C 315.1C 456 489 681 750

S19 209 223 324 073 263 309.1C 309.2C 315.1C 489 523D 524D 750

S20 223 073 200 215 309.1C 309.2C 315.1C 318 326 459.1C 489 750

S21 184 223 311 362 073 263 315.1C 489 750

S22 189 223 362 073 194 315.1C 489 523D 524D 750

S23 182C 183C 189 234 304 073 199 249D 263 309.1C 315.1C 523D 524D 750

S24 184 223 271 311 362 073 263 309.1C 315.1C 489 750

S25 129 223 362 073 152 263 315.1C 489 750

S26 093 129 223 249 362 073 152 263 315.1C 489 750

S27 189 194 195 223 227 278 362 073 263 315.1C 489 709 750

S28 093 179 223 288 298 327 073 195 249D 309.1C 309.2C 315.1C 489 595.1C 750

S29 129 157 188.1C 189 223 297 298 073 146 150 199 263 309.1C 309.2C 315.1C 489 750

S30 066 223 311 073 263 315.1C 489 573.1C 573.2C 573.3C 573.4C 709 750

S31 129 182C 183C 189 232A 249 304 311 344 073 152 249D 263 309.1C 309.2C 315.1C 523D 524D 750

S32 093 187 298 355 362 073 114 204 207 249D 263 309.1C 315.1C 15854G 16390 750

S33 184 214 223 362 073 263 309.1C 315.1C 489 709 750

S34 093 183C 189 220C 254 298 362 073 249D 263 315.1C 750

S35 223 362 073 146 183 263 315.1C 489 750

S36 209 223 324 073 263 315.1C 489 523D 524D 750

S37 182C 183C 189 217 268 288 311 073 263 309.1C 309.2C 315.1C 523D 524D 750

S38 066 223 311 073 263 309.1C 309.2C 315.1C 523D 524D 709 750

S39 129 162 212 304 073 249D 263 315.1C 523D 524D 548 750

S40 092 182C 183C 189 194C 195 217 261 266G 073 263 310D 523D 524D 16000 709 750

S41 223 224 245 292 263 073 146 263 315.1C 489 750

Y.J. Zhang et al. / Forensic Science International 151 (2005) 299–301 301S

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This paper follows the guidelines for publication of

population data requested by the journal [9].

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