guava publication list 2002-2011

7/28/2019 Guava Publication List 2002-2011

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2011)

AlgaeApplications

Comparative toxicity of a brominated flame retardant (tetrabromobisphenol A) onmicroalgae with single and multi-species bioassays. Debenest, T., et al.,Chemosphere (2011), doi:10.1016/j.chemosphere.2011.06.036Background: Chlorophyll autofluorescence and esterase activity were evaluated using athree-color Guava Easy-Cyte Plus System cytometerwith a laser emitting at 488 nm.The cytometer was calibrated each day of the experiment using the Guava check kit.

A Comprehensive GCMS Sub-Microscale Assay for Fatty Acidsand its Applications. Nicholas W. Bigelow, et al., J Am Oil Chem Soc, 1 February2011. This article is published with open access at Springerlink.com, DOI10.1007/s11746-011-1799-7Background: Flow cytometric measurements were made using a Millipore GuavaEasyCyte 8HT flow cytometer (Millipore, Billerica, MA, USA) Algal cells were stainedwith BODIPY 505/515 at different times during the cell cycle both to determine neutrallipid per cell by flow cytometry

AntibodyQuantitation,ScreeningandCharacterization

Identification of a sensitive anti-erythropoietin receptor monoclonal antibodyallows detection of low levels of EpoR in cells. Steve Elliott, et.al.,Journal of Immunological Methods 352 (2010) 1261392Flow cytometry on live cells with A82Cell viability and density were determined usingGuava ViaCount Assay reagents on a Guava EasyCyte

The Quality of Chimpanzee T-Cell Activation and Simian ImmunodeficiencyVirus/Human Immunodeficiency Virus Susceptibility Achieved via Antibody-Mediated T-Cell Receptor/CD3 Stimulation Is a Function of the Anti-CD3 AntibodyIsotype. Frederic Bibollet-Ruche, et. al., Journal of Virology, Oct. 2008, p. 1027110278 Vol. 82, No. 20Background: Antibody concentration in the supernatants was determined using aRapidQuant IgG assay (GUAVA Technologies. Flow cytometric analysis wasperformed using an EasyCyte flow cytometer (GUAVA, Hayward, CA).


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ApoptosisApplicationsAnnexinV&7AAD

Selective Killing of Mixed Lineage Leukemia Cells by a Potent Small-Molecule DOT1L

Inhibitor. Scott R. Daigle, et. al., Cell Press - Cancer Cell 20, 5365, July 12, 2011, ElsevierInc.Apoptosis was determined using the Guava Nexin Assay (Millipore 4500-0450)analyzedon a Guava EasyCyte Plus instrument (Millipore).

Stop codon read through with PTC124 induces palmitoyl-protein thioesterase-1 activity,

reduces thioester load and suppresses apoptosis in cultured cells from INCL

patients.Sarkar, C., Zhang, Z. & Mukherjee, A.B., Molecular Genetics and Metabolism (2011),doi: 10.1016/j.ymgme.2011.05.021 Background: Measurement of cellular apoptosis performedusing Guava Nexin assay kit (Millipore) which uses Annexin-V-PE for the identification ofapoptotic cells and 7-AAD for the detection of late stage apoptotic or dead cellsflow cytometryusing Guava EasyCyte Mini System (Millipore).

Myricetin induces pancreatic cancer cell death via the induction of apoptosis and

inhibition of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. P.A. Phillips et alCancer Letters., (2011), doi:10.1016/j.canlet.2011.05.002 Background: Measurement of

Annexin V positive cells Phosphatidylserine externalization was analyzed using the GuavaNexin Kit by flow cytometry

The Unfolded Protein Response in Human Corneal Endothelial Cells Following

Hypothermic Storage: Implications of a Novel Stress Pathway. W.L. Corwin, J.M. Baust,J.G. Baust, R.G. Van Buskirk, Cryobiology (2011)doi: 10.1016/j.cryobiol.2011.04.008Background: Counts of the unlabeled (live), necrotic (PI [1.5M]) and apoptotic (YOPRO-1[0.1M]) labeled cells were obtained using microfluidic flow cytometry (Millipore).

Combination Therapy with Epigallocatechin-3-Gallate and Doxorubicin in Human

Prostate Tumor Modeling Studies Inhibition of Metastatic Tumor Growth in Severe

Combined Immunodeficiency Mice. Stearns, Mark E. / Amatangelo, Michael D. / Varma,Devika / Sell, Chris / Goodyear, Shaun M., The American Journal of Pathology, 177 (6), p.3169,Dec 2010 doi:10.2353/ajpath.2010.100330Background:...Apoptosis was measured by flowcytometry by using Annexin V antibodies and a kit from Guava.

Casearin X exhibits cytotoxic effects in leukemia cells triggered by apoptosis. Ferreira,Paulo M. Pinheiro / Santos, Andr G. / Tininis, Aristeu G. / Costa, Patricia M. / Cavalheiro,

Alberto J. / Bolzani, Vanderlan S. / Moraes, Manoel O. / (...) / Pessoa, Cludia, Chemico-


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Biological Interactions, 188 (3), p.497, Dec 2010 doi:10.1016/j.cbi.2010.08.008 Background:All flow cytometry analyses were performed in a Guava EasyCyte Mini using...were analyzed byflow cytometry (EasyCyte from Guava Technologies)...was estimated by flow cytometry, usingthe Guava EasyCyte.

Pro-apoptotic activity of lipidic -amino acids isolated from Protopalythoa variabilis.

Wilke, Diego Veras / Jimenez, Paula Christine / Arajo, Renata Mendona / da Silva, WildsonMax Barbosa / Pessoa, Otlia Deusdnia Loiola / Silveira, Edilberto Rocha / Pessoa, Claudia /

Costa-Lotufo, Letcia Veras, Bioorganic & Medicinal Chemistry, 18 (22), p.7997, Nov 2010doi:10.1016/j.bmc.2010.09.027 Background: evaluated by flow cytometry (EasyCyte MiniSystem, Guava Technologies, CA, USA...

Role of Autophagy in Sonodynamic Therapy-Induced Cytotoxicity in S180 Cells. Wang,Xiaobing / Liu, Quanhong / Wang, Zhezhi / Wang, Pan / Zhao, Ping / Zhao, Xianghong / Yang,Liang / Li, Yixiang, Ultrasound in Medicine & Biology, 36 (11), p.1933, Nov 2010doi:10.1016/j.ultrasmedbio.2010.06.022 Background: ...(Guava easyCyte 8HT; Millipore)...lightwas measured by flow cytometry. Assay for apoptosis...apoptosis was performed by the GuavaNexin Assay (Millipore).

Triptolide and TRAIL Combination Enhances Apoptosis in Cholangiocarcinoma. Clawson,Kimberly A. / Borja-Cacho, Daniel / Antonoff, Mara B. / Saluja, Ashok K. / Vickers, Selwyn M.,Journal of Surgical Research, 163 (2), p.244, Oct 2010 doi:10.1016/j.jss.2010.03.067Background: ...Guava personal cell analysis flow cytometer (Guava Technologies, Hayward,CA). Cells...suspended cells with 100 L of the Guava Nexin reagent.

Role of Hsp-70 in Triptolide-Mediated Cell Death of Neuroblastoma.Antonoff, Mara B. /Chugh, Rohit / Skube, Steven J. / Dudeja, Vikas / Borja-Cacho, Daniel / Clawson, Kimberly A. /Vickers, Selwyn M. / Saluja, Ashok K., Journal of Surgical Research, 163 (1), p.72, Sep 2010doi:10.1016/j.jss.2010.04.047 Background: ...with annexin-V staining using Guava NexinReagent (Guava Technologies, Hayward, CA...manufacturer's instructions, and flow cytometry

was used for result...marker of early apoptosis, flow cytometry for annexin-V staining

Hepatitis B and Hepatitis C Virus Replication Upregulates Serine Protease Inhibitor

Kazal, Resulting in Cellular Resistance to Serine Protease-Dependent Apoptosis.

Lamontagne, J / Lu, X / Jason Lamontagne / Timothy M. Block / Xuanyong Lu , 84 (2): 907 --The Journal of Virology, Jul 2010 Background: For flow cytometry (fluorescence-activated cellsorting...and apoptosis was analyzed using a Guava EasyCyte Plus system (Guava


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Technologies)...Apoptotic cells were quantified by flow cytometry after double staining withannexin...

Sulforaphane induces DNA single strand breaks in cultured human cells. Sestili, Piero /Paolillo, Marco / Lenzi, Monia / Colombo, Evelin / Vallorani, Luciana / Casadei, Lucia / Martinelli,Chiara / Fimognari, Carmela, Mutation Research/Fundamental and Molecular Mechanisms ofMutagenesis, 689 (1-2), p.65, Jul 2010 doi:10.1016/j.mrfmmm.2010.05.003 Background:...Detection of apoptosis by flow cytometry Flow cytometric procedures were performed with a

Guava EasyCyte Mini flow cytometer...stained with 100l of Guava Nexin Reagent.

Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-

MB 231.Arunasree, K.M., Phytomedicine, 17 (8-9), p.581, Jul 2010doi:10.1016/j.phymed.2009.12.008 Background: ...carvacrol-treated cells were performed onGuava Easy Cyte Flow cytometer. Briefly, MDA-MB 231...with PBS, stained and analyzed byflow cytometry according to manufacturer's protocol...with Annexin V and analyzed by flowcytometer.

TBP Is Differentially Regulated by c-Jun N-Terminal Kinase 1 (JNK1) and JNK2 through

Elk-1, Controlling c-Jun Expression and Cell Proliferation. Zhong, S / Shuping Zhong /

Jody Fromm / Deborah L. Johnson. Molecular and Cellular Biology Jun 2010 Vol 27 (1): 54Background: DNA profiling determined by flow cytometry. Cells in earlyapoptosis...manufacturer's instructions (Guava Technologies, Inc.), with flow cytometry toquantify the percentage...

Adeno-associated virus induces apoptosis during co-infection with adenovirus. Timpe,Jennifer M. / Verrill, Kristin C. / Black, Bret N. / Ding, Han-Fei / Trempe, James P., Virology, 358(2), p.391, Feb 2007 doi:10.1016/j.virol.2006.08.042 Background: Annexin V-PE and 7-AADstaining was conducted using the GUAVA Nexin Kit Annexin V-PE and 7-AADand analyzedusing the Guava PCA flow cytometer.

Modulation of Human Neutrophil Functions In Vitro by Treponema denticola Major Outer

Sheath Protein. Puthengady, Thomas, Chun Xiang Sun / Elena Bajenova / Richard P. Ellen /Michael Glogauer, et al. -- Infection and Immunity. 74 (3): 1954, May 2010 Background:...granularity, were analyzed by flow cytometry (excitation wavelength...was measured with theGuava Nexin kit according to...manufacturer's protocol (Guava Technologies Inc.,Hayward...cells were analyzed with a Guava personal cytometer.


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2011)

Receptors Specific for the Carboxyl-Terminal Region of Parathyroid Hormone on Bone-

Derived Cells: Determinants of Ligand Binding and Bioactivity -- P. Divieti , A. I. Geller , G.Suliman , F. R. Bringhurst, Endocrinology. 146 (4): 1863, May 2010 Background: Apoptosiswas assessed by flow-cytometric detection of exposed...phycoerythrin-tagged annexin V(Guava Nexin method Guava Technologies, Hayward, CA)...resuspended in Nexin assay buffer(Guava Technologies).

Knockdown of COPA, Identified by Loss-of-Function Screen, Induces Apoptosis and

Suppresses Tumor Growth in Mesothelioma Mouse Model. Sudo, Hitomi / Tsuji, Atsushi B./ Sugyo, Aya / Kohda, Masakazu / Sogawa, Chizuru / Yoshida, Chisato / Harada, Yoshi-nobu /(...) / Saga, Tsuneo, Genomics, 95 (4), p.210, Apr 2010 doi:10.1016/j.ygeno.2010.02.002Background: Using flow cytometry, we then measured annexin...cells were determined by aGuava PCA system. (A) Representative flow cytometry dot plots at 48h after transfection...cellsusing microscopic and flow cytometric analyses, apoptosis...

Redox signaling via lipid raft clustering in homocysteine-induced injury of podocytes.

Zhang, Chun / Hu, Jun-Jun / Xia, Min / Boini, Krishna M. / Brimson, Christopher / Li, Pin-Lan,Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1803 (4), p.482, Apr 2010doi:10.1016/j.bbamcr.2009.12.006 Background: Flow cytometry detection of podocyte

apoptosis. The apoptosis rate of podocytes...and resuspension, the cells were detected using aflow cytometer (GUAVA, Hayward, CA, USA).

Lysophosphatidic acid induces upregulation of Mcl-1 and protects apoptosis in a PTX-

dependent manner in H19-7 cells. Sun, Yuanjie / Nam, Ju-Suk / Han, Dong-Hoon / Kim,Nam-Ho / Choi, Ho-Kyew / Lee, Jeong Kug / Rhee, Hae Jin / Huh, Sung-Oh, Cellular Signalling,22 (3), p.484, Mar 2010 doi:10.1016/j.cellsig.2009.11.002 Background: The cells were analyzedby flow cytometry using a Guava EasyCyteannexin V-Alexa Fluor 488 and PI, and analyzedby flow cytometry. The intensity of the green fluorescent annexin-Alexa488....Annexin V-PE andflow cytometry. Since membrane permeability...7-AAD, and analyzed by flow cytometry. We firstexamined the...and analyzed using the Guava PCA flow cytometer.

A Genome-wide In Vitro Bacterial-Infection Screen Reveals Human Variation in the Host

Response Associated with Inflammatory Disease. Dennis C. Ko, et. al., DOI10.1016/j.ajhg.2009.07.012. The American Journal of Human Genetics 85, 214227, August 14,2009 Background: Cells were stained with 7-AAD (eBioscience), and cell death and GFP-expressing bacteria were measured on a Guava Easycyte Plus flow cytometer.


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2011)

Cell surface staining of recombinant factor VIII is reduced in apoptosis resistant BHK-21

cells. Ishaque, Adiba / Thrift, John / Murphy, John E. / Konstantinov, K., Journal ofBiotechnology, 137 (1-4), p.20, Oct 2008 doi:10.1016/j.jbiotec.2008.07.1856Background: ...using the Guava Nexin kit (Guava Technologies, Hayward CA). Samples wereanalyzed by flow cytometry with a Guava PCA system (Guava Technologies)...solution priorto analysis by flow cytometry using the Guava PCA system.

Macrophage response to methacrylate conversion using a gradient approach. Lin, Nancy

J. / Bailey, LeeAnn O. / Becker, Matthew L. / Washburn, Newell R. / Henderson, Lori A., ActaBiomaterialia, 3 (2), p.163, Mar 2007 doi:10.1016/j.actbio.2006.10.001Background:...(Guava Nexin Apoptosis Assay, Guava Technologies, Hayward, CA). Usingthese dyes...fluorescence intensity from each dye were measured using a Guava PCA flowcytometer (Guava Technologies). Triplicate readings were taken from...

ApoptosisApplicationsCaspase

Selective Killing of Mixed Lineage LeukemiaCells by a Potent Small-Molecule DOT1L InhibitorScott R. Daigle, et. al., Cell Press - Cancer Cell 20, 5365, July 12, 2011.

Background: Percent of cells positive for activated caspases were determined byfollowing the protocol outlined in a kit specific for caspases 3 and 7 (Millipore

45000510). Samples were read on the Guava EasyCyte Plus instrument (Millipore).

Sanguinarine induces apoptosis of human osteosarcoma cells through theextrinsic and intrinsic pathways.Park, Hyunjin / Bergeron, Eric / Senta, Helena / Guillemette, Kim / Beauvais, Sabrina /

Blouin, Richard / Sirois, Jol / Faucheux, Nathalie, Biochemical and BiophysicalResearch Communications, 399 (3), p.446, Aug 2010doi:10.1016/j.bbrc.2010.07.114Background: ...sanguinarine was detected by flow cytometry using a MultiCaspase...sinstructions (Guava EasyCyte System, Guava Technologies...osteosarcoma cells.Guava Software images...multicaspase activation by flow cytometry.


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2011)

Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells toTRAIL-induced apoptosis.Niu, Ting-Kuang / Cheng, Yan / Ren, Xingcong / Yang, Jin-Ming, FEBS Letters, 584(16), p.3519, Aug 2010doi:10.1016/j.febslet.2010.07.018Background: caspase activity, the Guava multi-caspase detection protocol...analysisof caspase activity on a Guava flow cytometry.

Apoptogenic effect of 7,8-diacetoxy-4-methylcoumarin and 7,8-diacetoxy-4-methylthiocoumarin in human lung adenocarcinoma cell line: Role of NF-B, Akt,ROS and MAP kinase pathway. Goel, A. / Prasad, A. K. / Parmar, V.S. / Ghosh, B. /Saini, N. Chemico-Biological Interactions, 179 (2-3), p.363, May 2009doi:10.1016/j.cbi.2008.10.060Background: ...distribution was analyzed by flow cytometric analysis. . analyzed usingflow cytometryMulticaspase Assay Kit from Guava Technologies...were acquiredusing Guava Flow Cytometer.

ApoptosisApplicationsOxidativeStress

Oxidative stress induction by (+)-cordiaquinone J triggers both mitochondria-dependent apoptosis and necrosis in leukemia cellsMarinho-Filho, Jos Delano B. / Bezerra, Daniel P. / Arajo, Ana J. / Montenegro,Raquel C. / Pessoa, Claudia / Diniz, Jacio C. / Viana, Francisco A. / (...) / Costa-Lotufo, Letcia V., Chemico-Biological Interactions, 183 (3), p.369, Feb 2010doi:10.1016/j.cbi.2009.11.030Background: ...fragmentation was analyzed by flow cytometry (Guava EasyCyte)after...externalization was analyzed by flow cytometry after PS staining...Guava Nexin

Assay Kit was used...were analyzed by flow cytometry (EasyCyte from GuavaTechnologies)...

ROS accumulation and IGF-IR inhibition contribute to fenofibrate/PPAR -mediated inhibition of Glioma cell motility in vitro.Drukala, Justyna / Urbanska, Katarzyna / Wilk, Anna / Grabacka, Maja / Wybieralska,Ewa / Del Valle, Luis / Madeja, Zbigniew / Reiss, Krzysztof , Molecular Cancer, 9 (1),p.159, Jun 2010doi:10.1186/1476-4598-9-159


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Background: ...measurement was performed by flow cytometry based MitoPotentialKit...manufacturer protocol (Guava Easy Cyte). Loss of the...immediately analyzed byGuava EastCyte flowcytometer...Mito-Potential software (Guava Technologies, Inc...

Arginase 2 and nitric oxide synthase: Pathways associated with the pathogenesisof thyroid tumors. Sousa, Maria Sharmila A. / Latini, Flavia R.M. / Monteiro, Hugo P. /Cerutti, Janete M., Free Radical Biology and Medicine, 49 (6), p.997, Sep 2010doi:10.1016/j.freeradbiomed.2010.06.006

Background: ...of ROS was measured by flow cytometry using 2,7-dichlorofluorescein...(Guava Nexin method; Guava...EasyCyte Mini System flowcytometer (Guava Technologies).

ApoptosisApplicationsTUNELAssay

In vitro synergism between LFA-1 targeting leukotoxin (LeukotheraTM) andstandard chemother-apeutic agents in leukemia cells.Gupta A, et al. Leuk Res (2011), doi:10.1016, Journal Leukemia Research. 2011.05.017DNA fragmentation assayThe TUNEL assay was carried out using the TUNEL assaykit according to the suppliers instructions (Guava Technologies).

Sphonaxanthin, a marine carotenoid from green algae, effectively inducesapoptosis in human leukemia (HL-60) cells.Ganesan, Ponesakki / Noda, Kenji / Manabe, Yuki / Ohkubo, Takeshi / Tanaka,Yukihisa / Maoka, Takashi / Sugawara, Tatsuya / Hirata, Takashi, Biochimica etBiophysica Acta (BBA) - General Subjects, 1810 (5), p.497, May 2011doi:10.1016/j.bbagen.2011.02.008Background: ...by the Guava TUNEL assay using a Guava PCA according to themanufacturer's...concentrations was analyzed by flow cytometryTUNEL-positive cellswere measured by flow cytometry. (B) Carotenoid-treated HL-60...

Exogenous DNA uptake by bovine spermatozoa does not induce DNAfragmentation. Feitosa, W.B. / Mendes, C.M. / Milazzotto, M.P. / Rocha, A.M. /Martins, L.F. / Simes, R. / Paula-Lopes, F.F. / (...) / Assumpo, M.E.O.A.,Theriogenology, 74 (4), p.563, Sep 2010doi:10.1016/j.theriogenology.2010.03.016Background: Flow cytometry analyses (for TUNEL and SCSA) were performed usingthe Guava Easycyte Mini Flow Cytometry System (Guava Technologies, Hayward)


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BacterialApplications

Improvements of high-throughput culturing yielded novel SAR11 strains andother abundant marine bacteria form the Oregon coast and the Bermuda AtlanticTime Series study site. Stingle U, Tripp HJ, Giovannoni SJ. ISME Journal. 2007; 1,361-371.

Rapid microbial respiration of oil from the Deepwater Horizon spill in offshore

surface waters of the Gulf of Mexico. Bethanie R Edwards et al 2011 Environ. Res.Lett. 6 035301 Background:cells were counted using a Guava 5HT (Millipore) flowcytometer as described (Tripp 2008). Bacterial cells and Synechococcus cells weredifferentiated and counted using plots of side scatter versus yellow fluorescence andred fluorescence

A Genome-wide In Vitro Bacterial-Infection Screen Reveals Human Variation in

the Host Response Associated with Inflammatory Disease. Ko, Dennis C. / Shukla,Kajal P. / Fong, Christine / Wasnick, Michael / Brittnacher, Mitchell J. / Wurfel, Mark M. /Holden, Tarah D. / (...) / Miller, Samuel I., The American Journal of Human Genetics, 85

(2), p.214, Aug 2009 doi:10.1016/j.ajhg.2009.07.012Background: ...bacteria weremeasured on a Guava Easycyte Plus flow cytometer. Each LCL was assayed...with 7-

AAD and analyzed by flow-cytometry so that viability and intracellular bacteria...taggedwith inducible GFP and flow cytometry to allow for rapid and accurate...

Improvements of high-throughput culturing yielded novel SAR11 strains and

other abundant marine bacteria form the Oregon coast and the Bermuda Atlantic

Time Series study site. Stingle U, Tripp HJ, Giovannoni SJ., ISME Journal . 2007; 1,361-371. Background: Plates were screened after 4, 8 and 12 weeks with a GuavaEasyCyte cell counter (Guava Technologies, Hayward, CA, USA)...Screening of the

plates using the Guava EasyCyte, a 96-well flow cytometer primarily designed forcounting eukaryotic cells, is both fast and reliable. Counting of four 24-well plates takesapproximately 45 min. Limit of detection was determined with a pure culture ofCandidatus Pelagibacter ubique HTCC1062 and is in the range of 2000 cells/ml.

Counting marine microbes with Guava Easy-Cyte 96 well plate reading flow

cytometer. Tripp H J 2008, Nature Protocol Exchange., doi:10.1038/nprot.2008.29
http://iopscience.iop.org/1748-9326/6/3/035301http://iopscience.iop.org/1748-9326/6/3/035301http://iopscience.iop.org/1748-9326/6/3/035301http://iopscience.iop.org/1748-9326/6/3/035301


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2011)

Bioprocessing/QualityControl

Integrated Strategies for clone and Media Formulation Selection. Gerber MA,Arunakumari A, Huang D, Goldstein J, McNorton S, Drew B, Caple MV,Hartshorn JM,Wathen M, Kayser KJ. BioProcess International, January 2008, 58-63.

Cultivating anchorage-dependent cells: disposable bioreactor can grow animalcells immobilized on microcarriers. Costillo J, Vanhamel S. GEN. September 15,

2007; 40.

A capillary cytometer method to quantitate viable virus particles based on earlydetection of viral antigens and cellular events within single cells. Pheasey N, JohnJ, Love C, Coffin R, Ward JM, Boushaba R, Hoare M, Levy MS. Journal of VirologicalMethods. 2006; 137: 213-218.

A flow cytometric assay for rapid, accurate determination of baculovirus titers.Mulvania T, Hayes B, Hedin D. BioProcessing Journal: 2004;3(3); 47-53.

High throughput cytometric analysis of whole cells: Implication for cell therapy

product characterization. Hong K, McCaman M. Cell & Gene Therapy. May 2004;1(1): 1-10.

BioProcessing: Tactics for monitoring & delaying apoptosis. DePalma A. GEN.February 2004: 48-49.

Cell counting and viabiltiy assessments in the process development of cellulartherapeutics. Lem L. BioProcessing Journal.July/August 2003; 57-60.

CancerBiology

Anti-proliferative effect of LXR agonist T0901317 in ovarian carcinoma cells. Rough,James J / Monroy, M Alexandra / Yerrum, Smitha / Daly, John M , Journal of Ovarian Research,3 (1), p.13, May 2010 doi:10.1186/1757-2215-3-13Background: Flow Cytometric Analysis Aliquots...mini system by using the Guava CytoSoft CellCycle Program...manufacturer's instructions (Guava Technologies, Hayward).


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2011)

Inhibition of cell growth by Stellera chamaejasme extract is associated with induction of

autophagy and differentiation in chronic leukemia K562 cells. Tsolmon, Soninkhishig /Han, Junkyu / Isoda, Hiroko, Journal of Bioscience and Bioengineering, 110 (2), p.262, Aug2010 doi:10.1016/j.jbiosc.2010.02.006Background: ...CD14 was analyzed by flow cytometry (Guava Technologies, Inc...quantified byflow cytometry after cells were...analyzed on a flow cytometer (Guava technologies...werecounted by flow cytometry using cell viability...CD14-PE).

Role of KCNMA1 gene in breast cancer invasion and metastasis to brain. Khaitan, Divya /Sankpal, Umesh T / Weksler, Babette / Meister, Edward A / Romero, Ignacio A / Couraud,Pierre-Olivier / Ningaraj, Nagendra S , BMC Cancer, 9 (1), p.258, Jul 2009doi:10.1186/1471-2407-9-258Background: BK Ca channel green (FITC) fluorescence was recorded for a minimum of 5,000events using a Guava flow cytometer.

Resistance to cisplatin does not affect sensitivity of human ovarian cancer cell lines to

mifepristone cytotoxicity. Freeburg, Elizabeth M / Goyeneche, Alicia A / Seidel, Erin E /Telleria, Carlos M, Cancer Cell International, 9, p.4-4, Feb 2009doi:10.1186/1475-2867-9-4 Background: ...Mini microcapillary cytometer (Guava

Technologies)...cells are drawn into a capillary flow cell of known dimensions at...using theCytoSoft 4.1 software (Guava Technologies).

Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an

Anti-Estrogen Effect of Iodine. Stoddard II, Frederick R. / Brooks, Ari D. / Eskin, Bernard A. /Johannes, Gregg J., International Journal of Medical Sciences, 5 (4), p.189-196, Jul 2008Background: ...cells were grown on a six well plates, trypsinized, stained using ViaCountReagents (Guava Technologies, Hayward, CA) and analyzed using flow cytometry (GuavaEasyCyte Mini.).

The 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors, simvastatin, lovastatin

and mevastatin inhibit proliferation and invasion of melanoma cells. Glynn SA, O'SullivanDO, Eustace AJ, Clynes M, Odonnova N. BMC Cancer.2008;8:9.

Flavopiridol targets C-KIT transcription and induces apoptosis in gastrointestinal stromaltumor cells. Sambol EB, Ambrosini G, Geha RC, Kennealey PT, DeCarolis P, O'Connor R, WuYV,Motwani M, Chen JH, Schwartz GK, Singer S. Cancer Res. 2006; 66:(11).June 1, 2006.

RKIP sensitizes prostate and breast cancer cells to drug-induced apoptosis. Chatterjee D,Bai Y, Wang Z, Beach S, Mott S, Roy R, Braastad C, Sun Y, Mukhopadhyay A, Aggarwal BB,


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Darnowski J, Pantazis P, Wyche J, Fu Z, Kitagwa Y, Keller ET, Sedivy JM, Yeung KC. Journalof Biological Chemistry. 2004;279: 17515 - 17523.

The codon 72 polymorphic variants of p53 have markedly different apoptotic potential.Dumont, P, Leu, JI, Della Pietra, AC, George, DL, Murphy, M. Nature Genetics.2003;33(3):357-365.

Global transcriptional program of p53 target genes during the process of apoptosis andcell cycle progression. Mirza A, Wu Q, Wang LQ, McClanahan T, Bishop WR, Gheyas F, Ding

W, Hutchins B,Hockenberry T, Kirschmeier P, Greene JR, Liu SX. Oncogene.2003;22:3624-3654.

Response Markers and the Molecular Mechanisms of Action of Gleevec inGastrointestinal Stromal Tumors. Frolov A, Chahwan S, Ochs M, Arnoletti JP, Pan ZZ,Favorova O, Fletcher J, Von MehrenM, Eisenberg B, K. Godwin AK. Molecular CancerTherapeutics.2003;2: 699-709.

Critical Requirement of BAX for Manifestation of Apoptosis Induced by Multiple Stimuli inHuman Epithelial Cancer Cells. Wu Q, Kirschmeier P, Hockenberry T, Yang TY, Brassard DL,Wang L, McClanahan T,Black S, Rizzi G, Musco ML, Mirza A, Liu SX. Cancer Research.2002;62: 3373-3376.

Transcriptional Regulation during p21(WAF1/CIP1)-induced Apoptosis in Human OvarianCancer Cells. Wu Q, Kirschmeier P, Hockenberry T, Yang TY, Brassard DL, Wang L,McClanahan T,Black S, Rizzi G, Musco ML, Mirza A, Liu SX. Journal ofBiological Chemistry. 2002:277(39): 3632936337.

Protection of CLL B cells by a follicular dendritic cell line is dependent on induction ofMcl-1. Pedersen IM, Kitada S, Leoni LM, Zapata JM, Karras JG, Tsukada N, Kipps TJ, Choi YS,Bennett F, Reed JC. Blood. 2002;100(5): 1795-1801.

CellBiology

-Melanocyte stimulating hormone cytoprotective biology in human dermal fibroblastcells. Rebecca P. Hill, Paul Wheeler, Sheila MacNeil, John W. Haycock. Peptides.2005;26:1150-1158.

Hydrodynamic Shear Rate Regulates Melanoma-Leukocyte Aggregation, Melanoma

Adhesion to the Endothelium, and Subsequent Extravasation. Shile Liang et. al.,AnnBiomed Eng. 2008 April ; 36(4): 661671.


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(2002

2011)

doi:10.1007/s10439-008-9445-8.Background:After shear, aliquots were immediately fixed with 1% formaldehyde at roomtemperature and subsequently analyzed in GUAVA personal cytometer(GUAVA TechnologiesInc., Burlingame, CA). The size distribution and cellular composition of aggregates generated inthe cone-plate viscometer were determined by a two-color flow cytometric methodology

Hibiscus sabdariffa leaf induces apoptosis of human prostate cancer cells in vitro and in

vivo. Lin, H-H., Chan, K-C., Sheu, J-Y., Hsuan, S-W., Wang, C-J., Chen, J-H.,Food Chemistry

(2011)doi:10.1016/j.foodchem.2011.11.057Background: Mitochondrial transmembrane potential (MTP) assay. MTP of various cells wasstudied by staining the cells with JC-1, a metachromatic probe whichselectively enters themitochondria, using a standard protocol from the manufacturer. This probe exists in a redfluorescent monomeric form (~529 nm) in healthy mitochondria; but in depolarisedmitochondria, it diffuses to the cytoplasm and forms green fluorescent J-aggregates (~590 nm).In experiments requiring hyperosmolar stimulation, cells were exposed to hyperosmolar mediafor 24hr before trypsinisation. Trypsinised cells were resuspended in 1 mL of media andincubated with 10L of JC-1 dye for 30 min at a concentration recommended by themanufacturer. Subsequently, cells were analysed by a mini-flow cytometry method (GUAVA,

Millipore, Billerica, MA) and data analysed by ExpressPro software.

CellCountingandViability


Inhibitor. Scott R. Daigle, et. al., Cell Press - Cancer Cell 20, 5365, July 12, 2011, ElsevierInc.Background:Viable cell number was determined every 34 days for up to 18 days using theGuava Viacount assay (Millipore # 4000-0040) and analyzed on a Guava EasyCyte Plusinstrument (Millipore).

Fenofibrate suppresses microvascular inflammation and apoptosis through adenosinemonophosphateactivated protein kinase activation. Tomizawa, Atsuko / Hattori, Yoshiyuki/ Inoue, Teruo / Hattori, Sachiko / Kasai, Kikuo, Metabolism, 60 (4), p.513, Apr 2011doi:10.1016/j.metabol.2010.04.020 Background:...Apoptosis was evaluated using GuavaEasyCyte Mini Flow cytometry system (Millipore). The ViaCount...DNA-binding dyes in theGuava ViaCount Reagent. The nuclear...apoptotic, and dead cells.


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GuavaFlow

Cytometry

Publications

(2002

2011)

Effects of Non-Thermal Plasma on Mammalian Cells. Kalghatgi, Sameer / Kelly, Crystal M. /Cerchar, Ekaterina / Torabi, Behzad / Alekseev, Oleg / Fridman, Alexander / Friedman, Gary /

Azizkhan-Clifford, Jane, PLoS ONE, 6 (1), Jan 2011 doi:10.1371/journal.pone.0016270Background: ...propidium iodide (PI) and analyzed by Guava. Prior studies have focused mainlyon bactericidal...using a Cell Viability Assay (Guava EasyCyte Plus, Millipore, MA...Sampleswere analyzed immediately by flow cytometry (Guava EasyCyte Plus, Millipore, MA...

Polyethylene Glycol Modified-Albumin Enhances the Cold Preservation Properties of

University of Wisconsin Solution in Rat Liver and a Hepatocyte Cell Line 1. Abbas, Rime /Kombu, Rajan S. / Dignam, David / Gunning, William / Stulberg, Jonah J. / Brunengraber, Henri/ Sanabria, Juan R., Journal of Surgical Research, 164 (1), p.95, Nov 2010doi:10.1016/j.jss.2009.03.030 Background:Flow Cytometry Hepatocyte viability inthe...reperfusion was assessed by the Guava EasyCyte capillary flow cytometry system (FlowCore, Case Western Reserve...Cleveland, OH)Guava ViaCount assays (GuavaTechnologies).

Multiple shRNAs driven by U6 and CMV promoter enhances efficiency of antiviral effects

against foot-and-mouth disease virus. Kim, Su-Mi / Lee, Kwang-Nyeong / Lee, Su-Jung / Ko,Young-Joon / Lee, Hyang-Sim / Kweon, Chang-Hee / Kim, Hyun-Soo / Park, Jong-Hyeon,

Antiviral Research, 87 (3), p.307, Sep 2010doi:10.1016/j.antiviral.2010.06.004 Background:Cell viability test Flow cytometry, GuavaEasyCyte Plus system (Guava Technologies, Billerica, MA)using Guava ViaCont Reagent(Guava Technologies).

Impact of Mucosal Inflammation on Cervical Human Immunodeficiency Virus (HIV-1)-

Specific CD8 T-Cell Responses in the Female Genital Tract during Chronic HIV Infection.Pamela P. Gumbi / Nonhlanhla N. Nkwanyana / Alfred Bere / Wendy A. Burgers / Clive M. Gray,82 (17): 8529, Jun 2010 The Journal of Virology. Background:counted using an automatedGuava cell counter and Viacount staining (Guava).

Cigarette Smoke Induces Cellular Senescence Toru Nyunoya , Martha M. Monick ,Aloysius Klingelhutz , Timur O. Yarovinsky , Jeffrey R. Cagley , 35 (6): 681 -- American Journalof Respiratory Cell and Molecular Biology, May 2010. Background:...Personal Cytometer (PC)(Guava Technologies, Hayward, CA). The Guava ViaCount assay distinguishes...in theViaCount Reagent (Guava Technologies).


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GuavaFlow

Cytometry

Publications

(2002

2011)

Isopropylamine NONOate (IPA/NO) moderates neointimal hyperplasia following vascular

injury. Tsihlis, Nick D. / Murar, Jozef / Kapadia, Muneera R. / Ahanchi, Sadaf S. / Oustwani,Christopher S. / Saavedra, Joseph E. / Keefer, Larry K. / Kibbe, Melina R., Journal of VascularSurgery, 51 (5), p.1248, May 2010doi:10.1016/j.jvs.2009.12.028Background:(HUVEC) in Guava ViaCount Reagent (Guava Technologies, Hayward, Calif),and cell death assessed by Guava personal cell analysis (PCA...).

Hedgehog Signaling Promotes Medulloblastoma Survival via BclII. Eli E. Bar, / AneekaChaudhry, / Mohamed H. Farah, 170 (1): 347 -- American Journal of Pathology, May 2010[http://ajp.amjpathol.org/cgi/content/full/170/1/347] Background:...2 and 4 days aftercyclopamine treatment with the Guava-PCA flow cytometry system and ViaCount reagentaccording to the manufacturers instructions (Guava Technologies, Hayward, CA).

Tuning the cellular uptake and cytotoxicity properties of oligonucleotide intercalator-

functionalized mesoporous silica nanoparticles with human cervical cancer cells HeLa.

Vivero-Escoto, J.L. / Slowing, I.I. , Lin, Victor S.-Y., Biomaterials, 31 (6), p.1325, Feb 2010doi:10.1016/j.biomaterials.2009.11.009 Background:by a cell viability assay (GuavaViaCount), flow cytometry, and confocal fluorescence microscopy.

Global Demethylation of Rat Chondrosarcoma Cells after Treatment with 5-Aza-2 -

Deoxycytidine Results in Increased Tumorigenicity. Hamm, Christopher A. / Xie, Hehuang /Costa, Fabricio F. / Vanin, Elio F. / Seftor, Elisabeth A. / Sredni, Simone T. / Bischof, Jared / (...)/ Soares, Marcelo B., PLoS ONE, 4 (12), Dec 2009doi:10.1371/journal.pone.0008340 Background:Viability was determined using the GuavaEasyCyte Mini Flow Cytometry System, and the Guava ViaCount Reagent (Millipore; cat no.4000-0040).

Bioactive responses of Hep-G2 cells to soyasaponin extracts differs with respect to

extraction conditions. Zhang, Wei / Yeo, Mei Ching / Tang, Fang Yin / Popovich, David G.,

Food and Chemical Toxicology, 47 (9), p.2202, Sep 2009doi:10.1016/j.fct.2009.06.006 Background:..,Guava ViaCount reagentGuava flowcytometry system with CytoSoft...the dual fluorochrome flow cytometry cell viabilitymeasurement using Guava ViaCount


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GuavaFlow

Cytometry

Publications

(2002

2011)

Background:...2 and 4 days after cyclopamine treatment with the Guava-PCA flow cytometrysystem and ViaCount reagent according to the manufacturer's instructions (GuavaTechnologies, Hayward, CA).

CellCycle

DNA demethylation by 5-aza-2-deoxycytidine treatment abrogates 17 beta-estradiol -

induced cell growth and restores expression of DNA repair genes in human breast

cancer cells. K.P. Singh, J. Treas, T. Tyagi, W. Gao,Cancer Letters (2011)doi: 10.1016/j.canlet.2011.10.022 Background: For analysis of cell cycle by flow cytometry,200,000 cells from each treatment groups were collected and fixed in 70 % ethanol for aminimum of 24 hrs at 4oC. Samples were stained with Guava Cell Cycle Reagent (Millipore)and run on Guava EasyCyte Flow Cytometer (Millipore) instrument. A total of 5,000 events werecounted and data was acquired using GuavaSoft software (Millipore).

Both Xpa And DNA Polymerase Eta Are Necessary For The Repair Of Doxorubicin-

Induced DNA Lesions. M.C.S. Moraes, et. al., Cancer Letters (2011).doi: 10.1016/j.canlet.2011.09.019Background: Flow cytometry analysis (sub-G1 assay, cell cycle and _H2AX analysis)Data

were collected with a Guava Flow Cytometerand the percentageof sub-G1 populationdetermined with CytoSoft Data Acquisition and Analysis Software


Inhibitor. Scott R. Daigle, et. al., Cell Press - Cancer Cell 20, 5365, July 12, 2011.Background: cells were washed once more with PBS and stained with the Guava cell cyclereagent (Millipore 4500-0220) for 20 min. Samples were analyzed using the Guava EasyCytePlus System (Millipore).

The Fate of Chrysotile-Induced Multipolar Mitosis and Aneuploid Population in Cultured

Lung Cancer Cells. Beatriz de Araujo Cortez et. al., 2011, PLoS ONE 6(4): e18600.

doi:10.1371/journal.pone.0018600Background: cell cycle was analyzed by flow cytometry using the Guava System.

Hexane/ethanol extract of Glycyrrhiza uralensis and its active compound isoangustone A

induce G1 cycle arrest in DU145 human prostate and 4T1 murine mammary cancer cells.

Seon, Mi Ra / Park, So Young / Kwon, Soo Jin / Lim, Soon Sung / Choi, Hyun Ju / Park,Heesook / Lim, Do Young / Park, Jung Han Yoon, The Journal of Nutritional Biochemistry, InPress, Corrected Proof,Mar 2011


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GuavaFlow

Cytometry

Publications

(2002

2011)

doi:10.1016/j.jnutbio.2010.11.010Background:...Cell-cycle analysis by flow cytometry Cells were plated in...was conducted usinga Guava PCA-96 system).

Hydroxytyrosol rich extract from olive leaves modulates cell cycle progression in MCF-7

human breast cancer cells. Bouallagui, Zouhaier / Han, Junkuy / Isoda, Hiroko / Sayadi, Sami,Food and Chemical Toxicology, 49 (1), p.179, Jan 2011doi:10.1016/j.fct.2010.10.014

Background: Flow cytometry cell cycle analysis Briefly...distribution was recorded using aGuava Cell Analysis System...the arrested cells using flow cytometry.

A new tick Kunitz type inhibitor, Amblyomin-X, induces tumor cell death by modulating

genes related to the cell cycle and targeting the ubiquitin-proteasome system.

Chudzinski-Tavassi, Ana Marisa / De-S-Jnior, Paulo Luis / Simons, Simone Michaela / Maria,Durvanei Augusto / de Souza Ventura, Janaina / de Ftima Correia Batista, Isabel / Faria,Fernanda / Demasi, Marilene, Toxicon, 56 (7), p.1145, Dec 2010doi:10.1016/j.toxicon.2010.04.019Background:...M phase were analyzed by the cell cycle software Guava Express Plus (GuavaTechnologies).

Activation of Liver X Receptors and Retinoid X Receptors Induces Growth Arrest and

Apoptosis in Insulin-Secreting Cells. Wente et al. 148 (4): 1843 Endocrinology, Sep 2010Wolf Wente , / Martin B. Brenner , / Heike Zitzer , / Jesper Gromada / Alexander M. Efanov,Background:...Flow cytometry DNA content analysis MIN6...detected with the cytometer GuavaPCA system (Guava Technologies...cell cycle profile with flow cytometry after staining MIN6cells...cells was examined with flow cytometry using annexin V

Triptolide Induces Cell Death in Pancreatic Cancer Cells by Apoptotic and Autophagic

Pathways. Mujumdar, Nameeta / Mackenzie, Tiffany N. / Dudeja, Vikas / Chugh, Rohit /Antonoff, Mara B. / BorjaCacho, Daniel / Sangwan, Veena / Saluja, Ashok K.,

Gastroenterology, 139 (2), p.598, Aug 2010doi:10.1053/j.gastro.2010.04.046Background:...Analysis Cell cycle was analyzed using the Guava cell-cycle reagent and GuavaPCA flow cytometry according to the manufacturer's instructions (Guava Technologies).

From the Cover: Small-molecule MDM2 antagonists reveal aberrant p53 signaling in

cancer: Implications for therapy. Tovar et al. 103 (6): 1888 -- Science Signaling, June 2010Background:...was analyzed by BrdU labeling and flow cytometry. ( B ) Cell-cycle distribution


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GuavaFlow

Cytometry

Publications

(2002

2011)

was calculated from the flow cytograms in A and expressed as the was determined by theGuava Nexin kit and expressed as a percentage...

CBL Is Frequently Altered in Lung Cancers: Its Relationship to Mutations in MET and

EGFR Tyrosine Kinases. Tan, Yi-Hung Carol / Krishnaswamy, Soundararajan / Nandi,Suvobroto / Kanteti, Rajani / Vora, Sapana / Onel, Kenan / Hasina, Rifat / Salgia, Ravi, PLoSONE, 5 (1), Jan 2010doi:10.1371/journal.pone.0008972

Background:...Flow Cytometry Cell cycle analysis was carried out by flow cytometry.Approximately 210 6 cells were grown...cycle analysis was performed using a Guava PCA-96flow cytometer (Guava Technologies, Millipore).

Induction of apoptosis by thymoquinone in lymphoblastic leukemia Jurkat cells is

mediated by a p73-dependent pathway which targets the epigenetic integrator UHRF1.

Alhosin, Mahmoud / Abusnina, Abdurazzag / Achour, Mayada / Sharif, Tanveer / Muller,Christian / Peluso, Jean / Chataigneau, Thierry / Fuhrmann, Guy, Biochemical Pharmacology,79 (9), p.1251, May 2010doi:10.1016/j.bcp.2009.12.015Background:...Cellular DNA content was assessed by flow cytometry in either a Guava

EasyCyte Plus HP system.

ERBB2 suppression decreases cell growth via apoptosis in gastrointestinal

adenocarcinomas. Arrington, Amanda K. / Dahlberg, Peter S. / Davydova, Julia / Vickers,Selwyn M. / Yamamoto, Masato, Surgery, 146 (2), p.213, Aug 2009doi:10.1016/j.surg.2009.04.008 Background:...Guava PCA flow cytometer (GuavaTechnologies) according to manufacturer's instructions...Representation of cell cycle analysis byflow cytometry of all nontreated cells.

Disrupted Cell Cycle Control in Cultured Endometrial Cells from Patients with

Endometriosis Harboring the Progesterone Receptor Polymorphism PROGINS.

D'Amora, Paulo / Maciel, Thiago Trovati / Tambellini, Rodrigo / Mori, Marcelo A. / Pesquero,Joo Bosco / Sato, Helio / Giro, Manoel Joo Batista Castello / Schor, Eduardo, The AmericanJournal of Pathology, 175 (1), p.215, Jul 2009doi:10.2353/ajpath.2009.080966 Background:...fluorescence was analyzed using a GuavaPCA flow cytometer (Guava Technologies). Results were expressed as the...Changes in thenumber of cells were determined by flow cytometry using the Guava Viacount kit (GuavaTechnologies).


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GuavaFlow

Cytometry

Publications

(2002

2011)

Generation and characterization of JCV permissive hybrid cell lines. Sariyer, Ilker K. /Safak, Mahmut / Gordon, Jennifer / Khalili, Kamel, Journal of Virological Methods, 159 (1),p.122, Jul 2009doi:10.1016/j.jviromet.2009.02.023Background:...with the parental cells flow cytometry assay was performedFlow cytometricanalysis of...ethanol, stained with Guava Cell Cycle Reagent (Guava Technologies).

CellSurfaceExpression

A C-terminal PDZ binding domain modulates the function and localization of Kv1.3

Channels. Megan A. Doczi, et. al., Experimental Cell Research (2011).doi: 10.1016/j.yexcr.2011.06.009Background: Flow cytometry was done with the Easycyte single laser flow cytometer (GuavaTechnologies)Surface channel was measured by flow cytometry in cells transfected withKv1.3 or Kv1.3

Hepatitis C Virus Inhibits Cell Surface Expression of HLA-DR, Prevents Dendritic Cell

Maturation, and Induces Interleukin-10 Production. Saito et al. 82 (7): 3320, The Journal ofVirology. Ray, R / Kousuke Saito / Malika Ait-G / Steven M. Truscott / Keith Meyer, Jun 2010

Background: Cell surface expression...HLA-DR was quantified by flow cytometry. TransfectedHT1080 cells...light scatter) using a Guava Easycyte

Distinct role of the intracellular C-terminus for subcellular expression, shedding and

function of the murine transmembrane chemokine CX3CL1. Andrzejewski, Michael G. /Koelsch, Anne / Kogel, Tanja / Dreymueller, Daniela / Schwarz, Nicole / Ludwig, Andreas,Biochemical and Biophysical Research Communications, 395 (2), p.178, Apr 2010doi:10.1016/j.bbrc.2010.03.139Background: Flow cytometry HEK293 (5105 cells) were incubated...the cells was analyzedusing Guava EasyCyte flow cytometer (Millipore, Billerica...surface expression analysis by flowcytometry.

Cell surface staining of recombinant factor VIII is reduced in apoptosis-resistant BHK-21

cells. Ishaque A. Thrift J. Murphy J.E. Konstantinov K.Journal of Biotechnology (2008) 137:1-4 (20-27). Date of Publication: 10 Oct 2008Background: We have recently demonstrated an increase in recombinant factor VIII (rFVIII)secretion from BHK-21 cells (rBHK-21)The Zenon technique was used to double stain for cellsurface and intracellular rFVIII using flow cytometric Guava PCA(trademark) analysis


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GuavaFlow

Cytometry

Publications

(2002

2011)

Tissue specific characteristics of cells isolated from human and rat tendons and

ligaments. Scutt, N / Rolf, CG / Scutt, A, Journal of Orthopaedic Surgery and Research, 3,p.32-32, Jul 2008doi:10.1186/1749-799X-3-32Background:...the Guava Viacount systemabsorbance measured at 650 nm. Flow cytometricanalysis of cell surface markers Flow cytometry was used to assess the expression...washedthen analyzed on the Guava Personal Cytometry system...

Profiling of membrane protein variants in a baculovirus system by coupling cell-surfacedetection with small-scale parallel expression. Hanson, Michael A. / Brooun, Alexei / Baker,Kent A. / Jaakola, Veli-Pekka / Roth, Chris / Chien, Ellen Y.T. / Alexandrov, Alexander /Stevens, Raymond C., Protein Expression and Purification, 56 (1), p.85, Nov 2007doi:10.1016/j.pep.2007.06.003Background:...and assayed using a 96-well Guava EasyCyte microcapillary flow cytometer. Theraw data from a typical...internal receptor, and the Guava EasyCyte analysis detecting...surfaceexpression as measured by flow cytometry

DrugDiscovery

Converging Paths: New intersections of drug discovery and flow cytometry. Lem L,Cappione A. Innovations in Pharmaceutical Technology. 2006;32-36.

Agitation during lipoplex formation improves the gene knockdown effect of siRNA.

Barichello, Jose Mario / Kizuki, Shinji / Tagami, Tatsuaki / Asai, Tomohiro / Ishida, Tatsuhiro /Kikuchi, Hiroshi / Oku, Naoto / Kiwada, Hiroshi, International Journal of Pharmaceutics, InPress, Uncorrected Proof,Mar 2011doi:10.1016/j.ijpharm.2011.03.001Background: a flow cytometer Guava EasyCyte mini system (Guava...cell was directlyanalyzed in a flow cytometer Guava EasyCyte mini system.

Identification of AKN-032, a novel 2-aminopyrazine tyrosine kinase inhibitor, withsignificant preclinical activity in acute myeloid leukemia. Eriksson, Anna / Hglund, Martin/ Lindhagen, Elin / leskog, Anna / Hassan, Sadia Bashir / Ekholm, Carina / Fhlenhag, Karin /Parrow, Vendela, Biochemical Pharmacology, 80 (10), p.1507, Nov 2010doi:10.1016/j.bcp.2010.08.002Background: Flow cytometry analysis the effect of compounds...proliferation was alsoanalyzed using flow cytometryviability reagent (Guava ViaCount, staining dead cells).


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GuavaFlow

Cytometry

Publications

(2002

2011)

Cytotoxic activity of naphthoquinones with special emphasis on juglone and its 5-O-

methyl derivative. Montenegro, Raquel Carvalho / Arajo, Ana Jrsia / Molina, Mara Teresa /Filho, Jos Delano Barreto Marinho / Rocha, Danilo Damasceno / Lopz-Montero, Eulogio /Goulart, Marlia O.F. /Costa-Lotufo, Letcia Veras, Chemico-Biological Interactions, 184 (3),p.439, Mar 2010doi:10.1016/j.cbi.2010.01.041Background: ...Flow cytometry analysisFor all tested compounds, five thousand...HL-60 cellfluorescence was then determined by flow cytometry in a Guava EasyCyte Mine using Guava

Express Plus software...

Identification of Potent Chemotypes Targeting Leishmania major Using a High-

Throughput, Low-Stringency, Computationally Enhanced, Small Molecule Screen.

Sharlow, Elizabeth R. / Close, David / Shun, Tongying / Leimgruber, Stephanie / Reed, Robyn /Mustata, Gabriela / Wipf, Peter / (...) / Lazo, John S., PLoS Neglected Tropical Diseases, 3 (11),Nov 2009doi:10.1371/journal.pntd.0000540Background: ...ViaCount reagent (Guava Technologies, Hayward, CA) followed...parasites.Data were captured on a Guava EasyCyte Plus flow cytometer and analyzed using CytoSoft5.0.2 software (Guava Technologies).

A 96-well flow cytometric screening assay for detecting in vitro phospholipidosis-

induction in the drug discovery phase. Natalie, Mesens / Margino, Steemans / Erik, Hansen/ Annelieke, Peters / Geert, Verheyen / Philippe, Vanparys, Toxicology in Vitro, 23 (2), p.217,Mar 2009doi:10.1016/j.tiv.2008.11.010Background: ...on the Guava easycyte flow cytometer by analysing a defined...analysed of eachsample. On the Guava easycyte system, fluorescence and...respectively, was analysed. Flowcytometric built- in population statistics...

Stabilization of the Human 2-Adrenergic Receptor TM4TM3TM5 Helix Interface by

Mutagenesis of Glu1223.41, A Critical Residue in GPCR Structure. Roth, Christopher B. /Hanson, Michael A. / Stevens, Raymond C., Journal of Molecular Biology, 376 (5), p.1305, Mar2008doi:10.1016/j.jmb.2007.12.028 Background: ...cell-surface expression using a Guavacapillary flow cytometer


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GuavaFlow

Cytometry

Publications

(2002

2011)

Drug Discovery Poster:

Background: Apoptosis (programmed cell death, PCD) plays important roles in developmentand homeostasis of the human organism. Inappropriate apoptosis can lead to a wide range ofacute and chronic diseases.the use of microcapillary cytometry (Guava Technology)allowed us to screen in less than 90 days the whole in-house library.


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GuavaFlow

Cytometry

Publications

(2002

2011)

HIVMonitoring/HIVAIDS

Comparison of 5 flow cytometric immunophenotyping systems for absoluteCD4(+) T-lymphocyte counts in HIV-1-infected patients living inresource-limited settings.Pattanapanyasat K. Phuang-Ngern Y. Sukapirom K. Lerdwana S. Thepthai C.,Journal of Acquired Immune Deficiency Syndromes (2008) 49:4 (339-347). Dateof Publication: 1 Dec 2008

Background: CD4 (+) T-lymphocyte counts on blood samples from 150 HIV-1-infectedThai patients were determined in parallel by 5 FCM systems: the 2 single-platformvolumetric FCM systems, Guava

Evaluating new CD4 enumeration technologies for resource-constrainedCountries. Stevens W. Gelman R. Glencross D.K. Scott L.E. Crowe S.M. Spira T.Nature Reviews Microbiology (2008) 6:SUPPL. 11 (S29-S38). Date ofPublication: 2008

Evaluation of a single-platform microcapillary flowcytometer for enumeration of absolute CD4+ T-lymphocyte counts in HIV-1

infected Thai patients. Pattanapanyasat K, Phuang-Ngern Y, Lerdwana S,Wasinrapee P, Sakulploy N, Noulsri E, Thepthai C, McNicholl JM. Clinical Cytometry.2007; 72B: 387-396.Background: Various assays are used to enumerate peripheral blood absoluteCD4+ T-lymphocytes. Flow cytometry is considered the gold standard for thispurpose. However, the high cost of available flow cytometers and monoclonalantibody reagents make it difficult to implement such methods in theresource-poor settings. In this study, we evaluated a cheaper, recentlydeveloped single-platform microcapillary cytometer for CD4+ T-lymphocyteenumeration,.

Simpler tests for immune cells could transform AIDS care in Africa .Willyard, C. Nature Medicine.2007; 13 (10), 1131-1132.doi:10.1038/nm1007-1131.


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GuavaFlow

Cytometry

Publications

(2002

2011)

Comparison of capillary based microfluorometric assay for CD4+ T cell countestimation with dual platform flow cytometry. Thakar MR, Kishorekumar B, MahajanBA, Mehendale SM, Paranjape RS. AIDS Research and Therapy. 2006; 3:26.

A reliable and inexpensive EasyCD4 assay for monitoring HIV-infectedindividualsin resource-limited settings. Balakrishnan P, Solomon S, Mohanakrishnan J, Cecelia

A, Kumarasamy N, Murugavel KG, Venkatakrishnan B, Solomon S, Crowe SM, GaneshAK, Thyagarajan SP, Flanigan T, Mayer KH. Acquired Immune Deficiency Syndrome.

September 2006; 43:(1).Spacek LA, Shihab HM, Lutwama F, Summerton J, Mayanja H, Ronald A, Margolick JB,

Evaluation of a low-cost method, the Guava EasyCD4 Assay, to enumerate CD4-postive lymphocyte counts in HIV_infected patients in the UnitedStates and Uganda. Nilles TL, Quinn TC. Acquired Immune Deficiency Syndrome.2006; 41(5), 607-610.

Comparison of capillary based microfluorometric assay for CD4+ T cell countestimation with dual platform flow cytometry. Thakar MR, Kumar BK, Mahajan BA,Mehendale SM, Paranjape RS. AIDS Research and Therapy. 2006; 3(26).

Low-cost monitoring for HIV-infected individuals on highly active antiretorviraltherapy (HAART) in developing countries. Balakrishnan P, Solomon S, KumarasamyN, Mayer KH. Indian J Med Res.April 2005; 121, 345-355.

Comparison ofmicrocapillary cytometry technology and flow cytometry for CD4+and CD8+ T-cellestimation. Kandathil AJ, Kannangai R, David S, Nithyanandam,Solomon S, Balakrishnan P, Abraham OC, Subramanian S, Rupali P, Verghese VP,Pulimood S, Sridharan G. Clinical and Diagnostic Laboratory Immunology. August 2005;1006-1009.

Optimization of HIV-1 infectivity assays. Jones J, Whitford W, Wagner F, Kutsch O.Biotechniques. 2007; 43(5), 589-594.

Immunology

Isolation and characterization of T cells from semenOlivier, A.J., et al., J. Immunol. Methods (2011), doi:10.1016/j.jim.2011.11.001CD3+ cell counts were performed on a Guava cell counter using anti-


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GuavaFlow

Cytometry

Publications

(2002

2011)

CD3 PE monoclonal antibody (Guava Technologies, Hayward, CA, USA)CD3+ T cellnumbers in samples prior to any leukocyte isolation or enrichment technique weredetermined using a Guava cell counter and an anti-CD3+ PE stain, as described in themethods.

Augmentation of myelopoiesis in a murine host bearing a T cell lymphomafollowing in vivo administration of proton pump inhibitor pantoprazole,N.K. Vishvakarma, S.M. Singh, Biochimie (2011), doi: 0.1016/j.biochi.2011.06.022

Background: BMC population was analyzed for flow cytometry of 21 macrophagemarker F4/80 using easyCyte HT flow cytometer (Guava, USA).

Cryptococcus neoformans Directly Stimulates Perforin Production and RearmsNK Cells for Enhanced Anticryptococcal Microbicidal Activity -- Marr et al. 77 (6): 2436 -- Infection and Immunity. Kaleb J. Marr / Gareth J. Jones / Chunfu Zheng / Shaunna M.Huston / Martina Timm-McCann, Jan 2011Background: perforin labeling was measured by flow cytometry with a GuavaEasyCyte flow cytometer (Guava Technologies, San Francisco, CA). NK cells werelabeled with a phycoerythrin-Cy5-conjugated...

Antibodies against the voltage-dependent anion channel (VDAC) and itsprotective ligand hexokinase-I in children with autismGonzalez-Gronow, Mario / Cuchacovich, Miguel / Francos, Rina / Cuchacovich,Stephanie / del Pilar Fernandez, Maria / Blanco, Angel / Bowers, Edith V. / Pizzo,Salvatore V., Journal of Neuroimmunology, 227 (1-2), p.153, Oct 2010doi:10.1016/j.jneuroim.2010.06.001Background: Flow cytometry SK-N-SH cells were detached from...was performedimmediately prior to flow cytometric analysis to exclude dead cells. Flow cytometry wasconducted using the Guava EasyCyte Plus system.

In vitro human TGF- treatment converts CD4+CD25 T cells into induced Tregulatory like cellsShanmugasundaram, Revathi / Selvaraj, Ramesh K., Veterinary Immunology andImmunopathology, 137 (1-2), p.161, Sep 2010; doi:10.1016/j.vetimm.2010.04.017Background: dilution of CD4+CD25 cells by flow cytometry (Guava Eascyte,Millipore).

The effect of cholecyst-derived extracellular matrix on the phenotypic behaviourof valvular endothelial and valvular interstitial cells


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Brody, Sarah / McMahon, Jillian / Yao, Li / OBrien, Margret / Dockery, Peter / Pandit,Abhay, Biomaterials, 28 (8), p.1461, Mar 2007;doi:10.1016/j.biomaterials. 2006.11.030Background: ...analysing uptake of the antibody by flow cytometry (Guava ExpressPlus, Guava.... When analysed by flow cytometry, 81% of VECs expressed CD31...

Cross-Talk between Activated Human NK Cells and CD4+ T Cells via OX40-OX40Ligand Interactions. Zingoni A, Sornasse T, Cocks BG, Tanaka Y, Santoni A, and

Lanier L. Journal of Immunology. 2004;173: 3716-3724.

Megakaryocyte-bone Marrow Stromal Cell Aggregates Demonstrate IncreasedColony Formation and Alkaline Phosphatase Expression in Vitro. Miao D, MurantS, Scutt N, Genever P, Scutt A. Tissue Engineering. 2004; 10:5/6; 807-817.

Direct recognition of cytomegalovirus by activating and inhibitory NK cellreceptors.Arase H, Mocarski ES, Campbell AE, Hill AB, Lanier LL. Science2002;296(5571):1323-1326.

Inducible costimulator costimulates cytotoxic activity and IFN-gamma production

in activated murine NK cells. Ogasawara K, Yoshinaga SK, Lanier LL. Journal ofImmunology 2002:169(7): 3676-3685.

Cutting edge: the minor histocompatibility antigen H60 peptide interactswith both H-2Kb and NKG2D. Cerwenka A, O'Callaghan CA, Hamerman JA, Yadav R,

Ajayi W, Roopenian DC, Joyce S, Lanier LL. Journal of Immunology. 2002; 168(7):3131-3134.

NKG2D-mediated Natural Killer Cell Protection Against Cytomegalovirus IsImpaired by Viral gp40 Modulation of Retinoic Acid Early Inducible 1 GeneMolecules. Lodoen M, Ogasawara K, Hamerman JA, Arase H, Houchins JP, MocarskiES, Lanier LL. J. Exp. Med. 2003:197(10):1245-1253.

Impairment of NK cell function by NKG2D modulation in NOD mice. Ogasawara K,Hamerman JA, Hsin H, Chikuma S, Bour-Jordan H, Chen T, Pertel T, Carnaud C,Bluestone JA, Lanier LL. Immunity 2003;18(1):41-51.


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A Structural Basis for the Association of DAP12 with Mouse, but Not Human,NKG2D. Rosen DB, Araki M, Hamerman JA, Chen T, Yamamura T, Lanier LL. Journalof Immunology. 2004; 173: 2470-2478.

Serum-Free Expansion of CD34+ Umbilical cord Blood Using StemlineHematopoietic Stem Cell Expansion Medium. Allison D, Leugers S, Fuhr B, DonahueL, Swartzwelder F, Tario Jr. J, Harrington J, McNiece I. LifeScience Quarterly: ATechnical Application Newsletter. 2003; 4(1): 10-12.

Immunotoxicity

Development of a murine model of lymph node metastases suitable for immunotoxicity

studies. Egenolf, Devon D. / Rafferty, Patricia / Brosnan, Kerry / Walker, Mindi / Jordan, Jarrat/ Makropoulos, Dorie / Kavalkovich, Karl / (...) / Bugelski, Peter J., Journal of Pharmacologicaland Toxicological Methods, In Press, Corrected Proof, Dec 2010doi:10.1016/j.vascn.2010.12.001Background: Flow cytometry Purified anti-mouse CD16...The Guava EasyCyte Cell Toxicity Kit.

Parasitology

Vaccination with the ML0276 Antigen Reduces Local Inflammation but NotBacterial Burden during Experimental Mycobacterium leprae InfectionRaman et al. 77 (12): 5623 -- Infection and Immunity, Jun 2010Background: ..ViaCount assay with a PCA system (Guava Technologies, Hayward, CA).

Involvement of lectin pathway activation in the complement killing of GiardiaintestinalisEvans-Osses, Ingrid / Ansa-Addo, Ephraim A. / Inal, Jameel M. / Ramirez, Marcel I.,Biochemical and Biophysical Research Communications, 395 (3), p.382, May 2010doi:10.1016/j.bbrc.2010.04.025Background: Flow cytometry analysis Giardia intestinalis...parasites were analyzed ona Guava EasyCyte flow cytometer using ExpressPlus software (Guava Technologies).

Anti-IgG-FITC...and then subjected to flow cytometry analysis with antibodies...

Role of early lectin pathway activation in the complement-mediated killing ofTrypanosoma cruzi.Cestari, Igor dos S. / Krarup, Anders / Sim, Robert B. / Inal, Jameel M.,


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Molecular Immunology, 47 (2-3), p.426, Dec 2009doi:10.1016/j.molimm.2009.08.030Background: Flow cytometry analysis T. cruzi (1.0106 in 900l of...4C, washed inPBS and analysed with a Guava EasyCyte ExpressPlus flow cytometer (GuavaTechnologies).

Proteomics,NanoparticlesandBiomaterialsApplications

Oriented conjugates of single-domain antibodies and quantum dots: toward newgeneration of ultra-small diagnostic nanoprobesSukhanova Alyona, et. al.,Nanomedicine: Nanotechnology, Biology and Medicine (2011),doi:10.1016/j.nano.2011.07.007Background: Labeling of cells with sdAbs-QD conjugates and flow cytometrymeasurementsFlow cytometry measurements of stained cells were performed immediately afterstaining with awith a Guava EasyCyteTM Plus (Guava Technologies, Billerica, MA)flow cytometer. At least 5,000 events for each sample were collected. 488-nm argonlaser was used for excitation and fluorescence intensity was measured between570-

596 nm with Guava EasyCyte Plus

Enhancement of cell recognition in vitro by dual-ligand cancer targeting goldnanoparticlesLi, Xi / Zhou, Hongyu / Yang, Lei / Du, Guoqing / Pai-Panandiker, Atmaram S. / Huang,Xuefei / Yan, Bing, Biomaterials, 32 (10), p.2540, Apr 2011doi:10.1016/j.biomaterials.2010.12.031Background: Flow cytometry analyses were performed on a Guava EasyCyte Mini flowcytometry system (Millipore, Billerica, MA)...further supported by the CFM images andflow cytometry

MHC class I-presented lung cancer-associated tumor antigens identified byimmunoproteomics analysis are targets for cancer-specific T cell responseShetty, Vivekananda / Sinnathamby, Gomathinayagam / Nickens, Zacharie / Shah,Punit / Hafner, Julie / Mariello, Lisa / Kamal, Shivali / Philip, Ramila, Journal ofProteomics, Feb 2011doi:10.1016/j.jprot.2011.02.020


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Background: Peptide stabilization assay employing flow cytometry T2 cells were seededin a 24-well plate at 1...Cells were washed thrice with PBS and subjected to flowcytometry using Guava flow cytometer (Millipore).

Synthetic nanoparticle vaccines produced by layer-by-layer assembly of artificialbiofilms induce potent protective T-cell and antibody responses in vivoPowell, Thomas J. / Palath, Naveen / DeRome, Mary E. / Tang, Jie / Jacobs, Andrea /

Boyd, James G., Vaccine, 29 (3), p.558, Jan 2011doi:10.1016/j.vaccine.2010.10.001Background: ...analyzed immediately using a Guava EasyCyte Mini flow cytometer. Tomonitor uptake...PBS and analyzed by 2-color flow cytometryanalyzed by flowcytometry to detect donor CFSEhi and CFSElo.

Design and fabrication of multivalent Gal-containing quantum dots and study ofits interactions with asialoglycoprotein receptor (ASGP-R)Yang, Yang / Zhao, Yue-Tao / Yan, Ting-Ting / Yu, Min / Sha, Yin-Lin / Zhao, Zhi-Hui /Li, Zhong-Jun, Tetrahedron Letters, 51 (32), p.4182, Aug 2010doi:10.1016/j.tetlet.2010.06.002

Background: ...interaction with the ASGP-R were investigated using Flow cytometry(FCM) and fluorescence microscopyuptake of glyco-QDs was measured by FlowCytometry (Guava EasyCyte Mini System) and analyzed with the Guava ExpressPro.

Radiometric bimolecular beacons for the sensitive detection of RNA in singleliving cellsChen, Antony K. / Davydenko, Olga / Behlke, Mark A. / Tsourkas, Andrew, Nucleic

Acids Research, 38 (14), p.e148-e148, Aug 2010doi:10.1093/nar/gkq436Background: ...involving RBMB detection via flow cytometry, the second oligonucleotidewas...once in PBS and analyzed on a guava excite flow cytometer fitted with a 488 nmexcitation laser.

Biocompatibility of polymer grafted core/shell iron/carbon nanoparticlesMu, Qingxin / Yang, Lei / Davis, James C. / Vankayala, Raviraj / Hwang, Kuo Chu /Zhao, Jincai / Yan, Bing, Biomaterials, 31 (19), p.5083, Jul 2010doi:10.1016/j.biomaterials.2010.03.020Background: Flow cytometry analyses Flow cytometry analyses were performed on aGuava EasyCyte Mini flow cytometry system (Millipore, Billerica, MA).


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Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cellsThorek, Daniel L.J. / Tsourkas, Andrew, Biomaterials, 29 (26), p.3583, Sep 2008doi:10.1016/j.biomaterials.2008.05.015Background: ...groups washed with or without enzyme. 2.9. Flow cytometry andrelaxation measurements immediately after...particles were removed from T cellsamples, flow cytometry was performed on a Guava Easycyte (Guava Technologies,Hayward, CA...

Short-term effects of adhesion peptides on the responses of preosteoblasts topBMP-9Marquis, Marie-Eve / Lord, Etienne / Bergeron, Eric / Bourgoin, Lee,Biomaterials, 29 (8) p.1005, Mar 2008doi:10.1016/j.biomaterials.2007.10.047Background : ...pBMP-9 (400ng/mL) were detected after 48h by flow cytometry (GuavaEasyCyte System, Guava Technologies, Hayward, CA, USA). Briefly...according tothe manufacturer's instructions (Guava Nexin Assay Kit, Guava Technologies).

Interaction studies of novel cell selective antimicrobial peptides with model

membranes and E. coli ATCC 11775Joshi, Seema / Bisht, Gopal Singh / Rawat, Diwan S. / Kumar, Anil / Kumar, Rita / Maiti,Souvik / Pasha, Santosh, Biochimica et Biophysica Acta (BBA) - Biomembranes, 1798(10), p.1864, Oct 2010doi:10.1016/j.bbamem.2010.06.016Background: flow cytometer (Guava Technologies, Hayward, CA...Express pluscytosoft software (Guava Technologies, Hayward, CA...

Magnetic control of vascular network formation with magnetically labeledendothelial progenitor cellsWilhelm, C. / Bal, L. / Smirnov, P. / Galy-Fauroux, I. / Clment, O. / Gazeau, F. /Emmerich, J., Biomaterials, 28 (26), p.3797, Sep 2007doi:10.1016/j.biomaterials.2007.04.047Background: ...either in the Trypan blue exclusion test or by flow cytometry (GuavaViaCountR; calceine labeling). EPC proliferation...EPC phenotyping by quantitative flowcytometry.

CpG motifs in pDNA-sequences increase anti-PEG IgM production induced byPEG-coated pDNA-lipoplexes


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Tagami, Tatsuaki / Nakamura, Kazuya / Shimizu, Taro / Yamazaki, Naoshi / Ishida,Tatsuhiro / Kiwada, Hiroshi, Journal of Controlled Release, 142 (2), p.160, Mar 2010doi:10.1016/j.jconrel.2009.10.017Background: Cells were analyzed by using a flow cytometer, Guava EasyCyte Mini(Guava Technologies, CA, USA)means of double immunofluorescent staining andflow-cytometric analysis at day 2...

Sperm

Analysis

Sperm Proteasomes Degrade Sperm Receptor on the Egg Zona Pellucida duringMammalian FertilizationZimmerman SW, Manandhar G, Yi Y-J, Gupta SK, Sutovsky M, et al. (2011) PLoS ONE6(2): e17256.doi:10.1371/journal.pone.0017256Background: The effect of soluble zona proteins and proteasomal inhibitors on spermacrosomal status and formation of the acrosomal shrouds was assessed by flowcytometry with fluorescently labeled lectin PNA.Analysis was performed on a GuavaEasyCyte Plus flow cytometer (Guava Tech, Hayward, CA)

StemCellBiology

Hydrogen Sulfide Causes Apoptosis in Human Pulp Stem CellsKobayashi, Chie / Yaegaki, Ken / Calenic, Bogdan / Ishkitiev, Nikolay / Imai, Toshio / Ii,Hisataka / Aoyama, Izumi / (...) / Haapasalo, Markus, Journal of Endodontics, 37 (4),p.479, Apr 2011;doi:10.1016/j.joen.2011.01.017Background: ...The percentage of apoptotic cells was determined by Guava NexinReagent (Guava Technologies, Hayward, CA) by using 2 fluorescent...Apoptosis wasanalyzed by employing 2000 cells and Guava EasyCyte (Guava Technologies).

Identification of a stem-like cell population by exposing metastatic breast cancercell lines to repetitive cycles of hypoxia and reoxygenationLouie, Elizabeth / Nik, Sara / Chen, Juei-suei / Schmidt, Marlies / Song, Bo / Pacson,Christine / Chen, Xiu Fang / Chen, Emily I , Breast Cancer Research, 12 (6), p.R94,Nov 2010doi:10.1186/bcr2773


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Background:...C and in 5% carbon dioxide. Flow cytometry Non-confluent cultureswere...and cells were analyzed on a Guava EasyCyte Plus Flow Cytometer(Millipore)...microtiter plate and read by the Guava Flow Cytometer.

Inherent Differential Propensity of Dental Pulp Stem Cells Derived from HumanDeciduous and Permanent TeethGovindasamy, Vijayendran / Abdullah, Aimi Naim / Sainik Ronald, Veronica / Musa,Sabri / Che Ab. Aziz, Zeti Adura / Zain, Rosnah Binti / Totey, Satish / (...) / Abu Kasim,

Noor Hayaty, Journal of Endodontics, 36 (9), p.1504, Sep 2010doi:10.1016/j.joen.2010.05.006Background:...content was analyzed on Guava Technologies (Millipore, Billerica, MA)flow cytometer by using Cytosoft, Version 5.2, Guava Technologies software.

Cancer Stem Cells from human breast tumors are involved in spontaneousmetastases in orthotopic mouse modelsHuiping Liu et. al., PNAS, October 19, 2010, vol. 107, no. 42, 18115-18120www.pnas.org/cgi/content/short/1006732107Background:Guava flow cytometry analysis of the CD44* population (percentage)contained in the APTCs and the ITCs isolated from the L2G-TN1 breast tumor.

Expression of Multiple Stem Cell Markers in Dental Pulp Cells Cultured in Serum-free MediaHirata, Thais Miyuki / Ishkitiev, Nikolay / Yaegaki, Ken / Calenic, Bogdan / Ishikawa,Hiroshi / Nakahara, Taka / Mitev, Vanyo / Haapasalo, Markus, Journal of Endodontics,36 (7), p.1139, Jul 2010doi:10.1016/j.joen.2010.03.002Background:...Guava ViaCount reagent for 10 minutes and then analyzed by usingGuava EasyCyte flow cytometry (GE Healthcare Bioscience Co...analysis wereperformed by using Guava CytoSoft software (Guava Technologies).

Deciduous and Permanent Dental Pulp Mesenchymal Cells Acquire HepaticMorphologic and Functional Features In VitroIshkitiev, Nikolay / Yaegaki, Ken / Calenic, Bogdan / Nakahara, Taka / Ishikawa, Hiroshi/ Mitiev, Vanyo / Haapasalo, Markus, Journal of Endodontics, 36 (3), p.469, Mar 2010doi:10.1016/j.joen.2009.12.022Background: Flow Cytometry Cells were trypsinized with 0.05% trypsin solution...theseantibodies, the cells were analyzed with Guava EasyCyte flow cytometry (GuavaTechnologies)


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2011)

DNA methyltransferase inhibition induces mouse embryonic stem celldifferentiation into endothelial cellsBanerjee, Saswati / Bacanamwo, Methode, Experimental Cell Research, 316 (2), p.172,Jan 2010doi:10.1016/j.yexcr.2009.08.011Background:...enhanced chemiluminescence. Flow cytometry About 1106 cells wereblocked...hour, washed and analyzed in a Guava Easy Cyte flow cytometer using

Cytosoft 3.

Mesenchymal Stem Cells Derived From Canine UmbilicalCord VeinA Novel Source for Cell Therapy StudiesEder Zucconi et. al., Stem cells and Developmentdoi: 10.1089/scd.2008.0314Background:To analyze cell-surface expression of typical protein markers, cUCVadherent cells... total of 10,000 labeled cells were acquired using EasyCyte fl owcytometry (Guava Technologies).

Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord

Units: Cord is Different from BloodSecco, Mariane / Moreira, Yuri B. / Zucconi, Eder / Vieira, Natassia M. / Jazedje,Tatiana / Muotri, Alysson R. / Okamoto, Oswaldo K. / Zatz, Mayana, Stem CellReviews, 5 (4), p.387-401, Dec 2009doi:10.1007/s12015-009-9098-5Background:...using a Guava EasyCyte flow cytometer running Guava ExpressPlussoftware (Guava Technologies Hayward, CA). A panel of surface markers was tested byflow cytometry.

Human fallopian tube: a new source of multipotent adult mesenchymal stem cellsdiscarded in surgical proceduresJazedje, Tatiana / Perin, Paulo M / Czeresnia, Carlos E / Maluf, Mariangela / Halpern,Silvio / Secco, Mariane / Bueno, Daniela F / (...) / Zatz, Mayana , Journal ofTranslational Medicine, 7 (1), p.46, Jun 2009doi:10.1186/1479-5876-7-46Background:Analysis Flow cytometry analysis was performed using a Guava EasyCytemicrocapillary flow cytometer (Guava Technologies, Hayward, CA) utilizing laserexcitation...


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Stem cells from umbilical cord blood do have myogenic potential, with andwithout differentiation induction in vitroJazedje, Tatiana / Secco, Mariane / Vieira, Natssia M / Zucconi, Eder / Gollop, ThomazR / Vainzof, Mariz / Zatz, Mayana, Journal of Translational Medicine, 7, p.6-6, Jan 2009doi:10.1186/1479-5876-7-6Background:...cells was determined for flow cytometry...were analyzed using GuavaEasyCyte flow cytometer running Guava ExpressPlus software (Guava Technologies).

Early transplantation of human immature dental pulp stem cells from baby teethto golden retriever muscular dystrophy (GRMD) dogs: Local or systemic?Kerkis, Irina / Ambrosio, Carlos E / Kerkis, Alexandre / Martins, Daniele S / Zucconi,Eder / Fonseca, Simone AS / Cabral, Rosa M / Zatz, Mayana , Journal of TranslationalMedicine, 6 (1), p.35, Jul 2008doi:10.1186/1479-5876-6-35Background: (Guava Technologies, CA). Negative controls...antibody only. Resultswere analyzed using Guava Express Plus software (Guava Technologies, Hayward,CA, USA).

Age-related changes in human bone marrow-derived mesenchymal stem cells:

Consequences for cell therapiesStolzing, A. / Jones, E. / McGonagle, D. / Scutt, A., Mechanisms of Ageing andDevelopment, 129 (3), p.163, Mar 2008doi:10.1016/j.mad.2007.12.002Background: The cells were analysed using a Guava personal flow cytometry system(GUAVA Instruments, Hayward, USA).

Stem cells from umbilical cord blood differentiate into myotubes and expressdystrophin in vitro only after exposure to in vivo muscle environmentViviane A. NunesBiol. Cell (2007) 99, 185196doi:10.1042/BC20060075Background: floating (haematopoietic) and adherent (mesenchymal) cells werecharacterized with mouse anti-human PerCP (peridinin chlorophyll protein)-conjugatedCD34, FITC-conjugated CD45, PE (phycoerythrin)-conjugated CD117 (c-Kit), PE-Cy5-conjugated CD29 monoclonal antibodies... Appropriate gating was performed todetermine the molecular markers expression via flow cytometry (Guava Technologies).


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NTera2: A Model System to Study Dopaminergic Differentiation of HumanEmbryonic Stem Cells. Schwartz CM, Spivak CE, Shawn C. Baker SC, Timothy K.McDaniel TK, Jeanne F. Loring JF, Cuong Nguyen C, Francis J. Chrest FJ, RoberWersto RW, Ernest Arenas E, Xianmin Zeng X, William J. Freed WJ, and Mahendra S.Rao MS. Stem Cells andDevelopment. 2005:14:517-534.

TranslationalResearch

Flt3-ITD alters chemotherapy response in vitro and in vivo in a p53-dependentmanner. Pardee, Timothy S. / Zuber, Johannes / Lowe, Scott W., ExperimentalHematology, 39 (4), p.473, Apr 2011 doi:10.1016/j.exphem.2011.01.009...48 hours after the last infection by flow cytometry (Guava EasyCyte; GuavaTechnologies, Millipore, USA)

Suboptimal adherence associated with virological failure and resistancemutations to first-line highly active antiretroviral therapy (HAART) in Bangalore,India. Ekstrand, Maria L. / Shet, Anita / Chandy, Sara / Singh, Girija / Shamsundar,Ranjani / Madhavan, Vidya / Saravanan, Shanmugam / Kumarasamy,Nagalingeswaran, International Health, 3 (1), p.27, Mar 2011doi:10.1016/j.inhe.2010.11.003...cell counts were performed on whole-blood specimens collected in an EDTA tubeusing a single platform flow cytometry assay (PCA system; Guava Technologies Inc.,Hayward, CA, USA).

Stability and transport of cervical cytobrushes for isolation of mononuclear cellsfrom the female genital tractLiebenberg, Lenine J. / Gamieldien, Hoyam / Mkhize, Nonhlanhla N. / Jaumdally,Shameem Z. / Gumbi, Pam P. / Denny, Lynette / Passmore, Jo-Ann S., Journal ofImmunological Methods, Feb 2011 doi:10.1016/j.jim.2011.01.013...enumeration using a Guava automated cell...and used for flow cytometry analysisof...for automated Guava cell counting...viability by flow cytometry.

Quality assurance of intracellular cytokine staining assays: Analysis of multiplerounds of proficiency testingJaimes, Maria C. / Maecker, Holden T. / Yan, Ming / Maino, Vernon C. / Hanley, MaryBeth / Greer, Angela / Darden, Janice M. / D'Souza, M. Patricia, Journal ofImmunological Methods, 363 (2), p.143, Jan 2011 doi:10.1016/j.jim.2010.08.004


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...techniques in that this flow-based application simultaneously...performing QualityAssurance of flow cytometry assays and reporting data...was determined using theGuava ViaCount assay (Guava...staining variables, flow cytometer used, acquisition)...

Quality Monitoring of HIV-1-Infected and Uninfected Peripheral BloodMononuclear Cell Samples in a Resource-Limited Setting Olemukan et al. 17 (6): 910, Clinical and Vaccine Immunology Dec 2010...blue exclusion, or by a Guava PCA machine (Guava Technologies...Hayward, CA),

using Guava ViaCount reagent. PBMC were...run on a dual-laser flow cytometer usingthe single-platform...

Evaluation of a Quantitative Real-Time PCR Assay to Measure HIV-SpecificMucosal CD8+ T Cell Responses in the CervixChege, Duncan / Chai, Yijie / Huibner, Sanja / McKinnon, Lyle / Wachihi, Charles /Kimani, Makubo / Jaoko, Walter / Rebbapragada, Anuradha, PLoS ONE, 5 (10), Oct2010 doi:10.1371/journal.pone.0013077...addressed by enumerating T cell subsets via flow cytometry assays, such as theGuava automated cell counter

FOXP3 Expression Is Upregulated in CD4 + T Cells in Progressive HIV-1 Infectionand Is a Marker of Disease SeveritySuchard, Melinda S. / Mayne, Elizabeth / Green, Victoria A. / Shalekoff, Sharon /Donninger, Samantha L. / Stevens, Wendy S. / Gray, Clive M. / Tiemessen, Caroline T.,PLoS ONE, 5 (7), Jul 2010 doi:10.1371/journal.pone.0011762...counted using a capillary cytometer (Guava technologies)

Disrupted Cell Cycle Control in Cultured Endometrial Cells from Patients withEndometriosis Harboring the Progesterone Receptor Polymorphism PROGINS --D'Amora et al. 175 (1): 215 -- American Journal of PathologyPaulo D' / Thiago Trovati / Rodrigo Tambellini / Marcelo A. Mori / Bosco Pesquero, Jul2010. Background:...fluorescence was analyzed using a Guava PCA flow cytometer(Guava Technologies).Changes in the number of cells were determined by flowcytometry using the Guava Viacount kit (Guava Technologies).

Potent orally bioavailable purine nucleoside phosphorylase inhibitor BCX-4208induces apoptosis in B- and T-lymphocytesA novel treatment approach forautoimmune diseases, organ transplantation and hematologic malignancies


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Bantia, Shanta / Parker, Cynthia / Upshaw, Ramanda / Cunningham, Amanda / Kotian,Pravin / Kilpatrick, J. Michael / Morris, Philip / Babu, Yarlagadda S., InternationalImmunopharmacology, 10 (7), p.784, Jul 2010 doi:10.1016/j.intimp.2010.04.009...Two-color flow cytometry analysis was performed using the Guava EasycytePlus...Three-color flow cytometry analysis was performed using Guava EasycytePlus.

Concordant Proficiency in Measurement of T-Cell Immunity in Human

Immunodeficiency Virus Vaccine Clinical Trials by Peripheral Blood MononuclearCell and Enzyme-Linked Immunospot Assays in Laboratories from ThreeContinentsBoaz et al. 16 (2): 147, Clinical and Vaccine Immunology, Gilmour, J / Peter Hayes, Jun2010. Background: ...of enzyme-linked immunospot (ELISPOT) and flow cytometryassays which determine the number of antigen-specificGuava personal cell analysissystem..

Usefulness of Alternate Prognostic Serum and Plasma Markers for AntiretroviralTherapy for Human Immunodeficiency Virus Type 1 InfectionKannangai et al. 15 (1): 154 -- Clinical and Vaccine Immunology [83K]

Sridharan, G / R. Kannangai / A. J. Kandathil / D. L. Ebenezer, May 2010...CD4 T-cell counts were estimated with the Guava EasyCD4 System

Impact of Mucosal Inflammation on Cervical Human Immunodeficiency Virus(HIV-1)-Specific CD8 T-Cell Responses in the Female Genital Tract during ChronicHIV InfectionGumbi et al. 82 (17): 8529, The Journal of Virology, Pamela P. Gumbi / Nonhlanhla N.Nkwanyana / Alfred Bere / Wendy A. Burgers, May 2010counted using an automated Guava cell counter and Viacount staining (Guava).

Comparison of Microcapillary Cytometry Technology and Flow Cytometry forCD4+ and CD8+ T-Cell EstimationKandathil et al. 12 (8): 1006 ,Clinical and Vaccine Immunology , May 2010...Microcapillary Cytometry Technology and Flow Cytometry for CD4 and CD8 T-Cell...microcapillary technique (Guava Technologies).

Ginseng (Panax quinquefolius) Reduces Cell Growth, Lipid Acquisition andIncreases Adiponectin Expression in 3T3-L1 CellsYeo et al., Evidence-based Complementary and Alternative Medicine, May 2010


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...Cells were mixed with the Guava ViaCount reagent which can distinguish

CD57 expression by T cells in the female genital tract of HIV-zx1 infected womenLiebenberg, Lenine J. / Adedeji, Adebayo Lawrence / Martin, Darren P. / Gumbi, Pam P./ Denny, Lynette / Passmore, Jo-Ann S., Clinical Immunology, 135 (1), p.137, Apr 2010doi:10.1016/j.clim.2009.12.007...phenotyping using an automated Guava cell counter (Guava technologies)marker, CD3-PE (T cells; Guava technologies).

Validation of Microcapillary Flow Cytometry for Community-Based CD4+ TLymphocyte Enumeration in remote Burkina FasoCybele A Renault, et. al., The Open AIDS Journal, 2010, 4, 171-175parallel CD4+ measurements were performed using microcapillary (GuavaEasyCD4) EasyCD4 system was feasible and highly accurate in the harsh conditionsof this remote city in Sub-Saharan Africa, demonstrating acceptable sensitivity andspecificity

Reduced transcription of TCOF1 in adult cells of Treacher Collins syndromepatients

Masotti, Cibele / Ornelas, Camila C / Splendore-Gordonos, Alessandra / Moura, Ricardo/ Flix, Tmis M / Alonso, Nivaldo / Camargo, Anamaria A / Passos-Bueno, Maria Rita ,BMC Medical Genetics, 10 (1), p.136, Dec 2009doi:10.1186/1471-2350-10-136... Flow cytometry Cells were harvested with...anti-mouse-PE secondary antibody(Guava Technologies) for additional...acquired with an EasyCyte flow cytometer (GuavaTechnologies...in mesenchymal cells. Flow cytometry results showed that most...

Feasibility investigation of allogeneic endometrial regenerative cellsZhong, Zhaohui / Patel, Amit N / Ichim, Thomas E / Riordan, Neil H / Wang, Hao / Min,Wei-Ping / Woods, Erik J / (...) / Minev, Boris, Journal of Translational Medicine, 7, p.15-15, Feb 2009 doi:10.1186/1479-5876-7-15...Technologies, inc. Hayward, CA (Guava flow cytometer). Only samples with >90...CD45 and CD34 negative (< 5%) by flow cytometry; (v) Cell viability > 95% bytrypan blue staining and Guava flow cytometer...


44/52

GuavaFlow

Cytometry

Publications

(2002

2011)

Tissue specific characteristics of cells isolated from human and rat tendons andligaments. Scutt, N / Rolf, CG / Scutt, A , Journal of Orthopaedic Surgery andResearch, 3 (1), p.32, Jul 2008 doi:10.1186/1749-799X-3-32...the Guava Viacount system (Guava Technologies) Flow cytometric analysis of cellsurface markers Flow cytometry was used to assess the expression...then analysed inthe Guava Personal Cytometry system...

Rapid, high throughput determination of cervical cytology specimen adequacy

using a capillary-based cytometer. Polina R. Sturgis C. Patterson J. Patterson B.K.Cytometry Part B - Clinical Cytometry (2008) 74:2 (133-136), March 2008More than 100 liquid-based cervical cytology specimens collected in ThinPrep vialswere processed into slides for diagnosis and assessment of specimenadequacyResidual liquid (100 (mu)L) was transferred to a single well of a 96-wellplate and run in a capillary cytometer (Guava Technologies, Hayward, CA) with forwardscatter and side scatter detectors.

Toxicity/Genotoxicity

Sensitive DsRed fluorescence-based reporter cell systems for genotoxicity andoxidative stress assessment.Hendriks, Giel / Atallah, Mirna / Raamsman, Martine / Morolli, Bruno / van der Putten,Haidy / Jaadar, Haziz / Tijdens, Ine / Vrieling, Harry, Mutation Research/Fundamentaland Molecular Mechanisms of Mutagenesis, Mar 2011doi:10.1016/j.mrfmmm.2011.02.013Background: ...was determined using flow cytometry (Guava easyCyte 6HT,Millipore...viability was determined by flow cytometry.

Protective effect of creatine against RNA damage.Fimognari, Carmela / Sestili, Piero / Lenzi, Monia / Cantelli-Forti, Giorgio / Hrelia,Patrizia, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,670 (1-2), p.59, Nov 2009, doi:10.1016/j.mrfmmm.2009.07.005

Highly hydroxylated fullerene localizes at cytoskeletons and inhibits oxidative

stress in adipocytes and a subcutaneous adipose-tissue equivalent. Li Xiao,Hisae Aoshima, Yasukazu Saitoh, Nobuhiko Miwa. Free Radical Biology and Medicine,In Press, Accepted Manuscript, Available online 27 May 2011
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guava publication list 2002-2011

Documents