grds international conference on health and life (17)
TRANSCRIPT
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VITAMINE E ROLE ON DUODENUM HISTOLOGICAL STRUCTURE OF WHITE RAT (Rattus norvegicus, Berkenhout,
1769) UNDER CADMIUM EXPOSURE
Shadrina Fildzah Ramadhani
Counselor: Drs. Yohanes Sugiyanto, M.S.Faculty of Biology
Universitas Gadjah Mada, Indonesia
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Introduction
Vitamin E as antioxidant
Cadmium toxicity
Cadmium (Cd)
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Cadmium effects inside
the body
• Ingested cadmium is poorly absorbed.
• Cadmium does not undergo metabolic conversion
• Given orally, cadmium chloride is absorbed quickly in the lower side of oral and digestive tract lamina mucosa
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Tunica Submucosa
Tunica Serosa
Tunica Mucosa
Tunica Muscularis
(Kuehnel, 2003)
Figure 1. Histological structure of Duodenum
Duodenum
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Vitamin E
• Vitamin E has been shown, in some studies, to inhibit lipid peroxidation, and forms an integral part of the cell membrane
• Vitamin E is able to protect biological membranes against free radical damage
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Objective
The aims of the study is to determine the effect of cadmium on the duodenum histological structure of
white rat (Rattus norvegicus, Berkenhout, 1769) and the dose of vitamin E as antitoxic.
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Methods
Instrumentation
Materials
• Mayer’s albumin• Xilol• Hematoksilin• Eosin• Canada Balsam
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18 white rats (Rattus norvegicus Bekenhout,1769)
Given treatmentfor 4 weeks
Aquades 1 ml/day (K1)
CdCl2 1 ml at dose 1,5 mg/kg bw/day (K2)
Vitamin E 6,2 IU (K3)
Treatment
CdCl2 1 ml at dose 1,5 mg/kg bw/day + vitamin
E 3,15 IU (P1)CdCl2 1 ml at dose 1,5
mg/kg bw/day + vitamin E 6,2 IU (P2)
CdCl2 1 ml at dose 1,5 mg/kg bw/day +
vitamin E 12 IU (P3)Euthanasia,
NecropsyPreparations and
scoring
Control
Methods
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Observation
Damage on Duodenum
• Degeneration of Lamina Propria
• Infiltration of Leukocytes • Dilated Gland• Villi Fusion• Edema
Scoring system
• 0 = normal, damage absent• 1 = low, damage present, not in all coupes, not
in all rats• 2 = moderate, damage present, in all coupes,
not in all rats; not in all coupes, in all rats• 3 = high, damage present, in all coupes, in all
rats
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Results
Table 1. Scoring damage on Control Group
Damage K (1) 1 K (1) 2 K (1) 3 K (2) 1 K (2) 2 K (2) 3 K (3) 1 K (3) 2 K (3) 3
Degeneration of Lamina Propria 0 0 0 2 2 2 0 0 0
Infiltration of Leukocytes 0 0 0 1 1 2 0 0 0
Dilated Gland 0 0 1 2 1 1 1 0 0
Villi Fusion 1 0 0 2 2 2 0 0 0
Edema 0 0 0 1 1 0 0 0 0
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Table 2. Scoring damage on Treatment Group
Damage P(1) 1 P (1) 2 P (1) 3 P (2) 1 P(2) 2 P(2) 3 P (3) 1 P(3) 2 P (3) 3
Degeneration of Lamina Propria 0 0 1 1 0 0 0 0 1
Infiltration of Leukocytes 1 0 0 0 0 0 0 0 0
Dilated Gland 1 0 1 0 0 1 1 1 0
Villi Fusion 1 1 1 1 0 0 0 1 0
Edema 0 1 0 0 0 0 0 0 0
Results
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A B
Figure 2. Duodenum Histological Structure of Control Group (A) Aquades dan (B) Vitamin E (10x10)
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Figure 3. Damages by Cadmium on Histological Structure of Duodenum (A) Degeneration of Lamina Propria (B) Infiltration of
Leukocytes (C) Dilated Gland (D) Villi Fusion (E) Edema(10x40)
A C
D E
B
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A B
C
Figure 4. Histological Structure of Tteatment Group (A) Cadmium + vitamin E 3,15 IU (B) Cadmium + vitamin E
6,2 IU (C) Cadmium + vitamin E 12 IU (10x10)
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Cadmium
• Causes inflamation on intestine mucose
• Uncontrolled poiferation of intestinum cells
• Stacking of fluid in the interstitial tissue
Vitamin E
• Vitamin E, are able to interact with oxidizing radicals directly.
• Vitamin E terminates the chain reaction of lipid peroxidation in bio-membranes and lipoproteins
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Conclusion
Vitamin E repair the damage of histological structure on duodenum under cadmium expousre of white rats
The optimal dose of vitamin E that can repair the damage is 6,2 IU
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Acknowledgement
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Thank You