GRADE 10, OSMOSIS LAB

1. Prepare 300 ml of the following solutions: 0.0 Molar sucrose, 0.3 Molar sucrose, 0.5 Molar sucrose, 1.0 Molar sucrose. The molecular formula of sucrose is C12H220H. 1.0 Molar is the equivalent of 1 mole/ dm3.

2. Using a scalpel gently scrape off some tissue from the inside of a potato. Place the scraping on a clean slide. Place a drop of Iodine solution. Place a cover slip.

3. Observe the cells under high magnification. Record your observations.

4. Cut 4 pieces of potato chips each measuring 5 cm X 1 cm X 1 cm. Make these measurements as accurate as possible. You should be able to measure to ± 1mm. Leave no skin on the potato chips.

5. Place 1 chip in each of the sucrose solutions. Their lengths may be slightly different so keep track of which chip goes into which solution.

6. Leave them for 2-3 days.

7. Remove each chip from their respective solution.

8. Measure the length of each chip.

9. Calculate the % change in the length of each chip.

10. Using a scalpel gently scrape off some tissue from each chip. Place the scraping on a clean slide. A separate slide is required for each. Place a drop of Iodine solution. Place a cover slip.

11. Observe the cells under high magnification. Record your observations.

You will be working in pairs.