glycolysis and kreb cycle
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glycolysis and kreb cycleTRANSCRIPT
PresentationOn
“Glycolysis And Kreb Cycle”
Presented by:-AAKRITI
GFT/115201
# The overall reaction:-C6H12O6+6O2 6CO2+6H2O+Energy(~30-32ATP)
# Definition:- It is a process of taking oxygen
and glucose and turning them into ATP i.e. energy for the body.
# Efficiency:- It is a 40% efficient process with 60% of the energy going to heat.
# Happens in 3 phases:-a) Glycolysis b) Krebs's Cycle c) ETC
Cellular Respiration
PATHWAY EUKARYOTE PROKARYOTE
GLYCOLYSIS CYTOPLASM CYTOPLASM
INTERMEDIATE STEP CYTOPLASM CYTOPLASM
KREB’s CYCLE MITOCHONDRIAL MATRIX CYTOPLASM
ETC MITOCHONDRIAL INNER MEMBRANE
PLASMA MEMBRANE
Where does cellular respiration take place?
STRUCTURE OF CELL
REVIEW:-# Smooth outer
membrane.# Folded inner
membrane.# Folds called
cristae.# Space inside
cristae is called matrix.
STRUCTURE
MITOCHONDRIA
OVERALL PROCESS DIAGRAM
Derived from Greek words; Glykys=sweet & Lysis=splitting
It is an anaerobic process. Ten step metabolic pathway to convert glucose into two molecules of pyruvate and
two molecules each of NADH and ATP. It occurs in the cytoplasm of plant and
animal cells. Free energy released in this process is
stored as two molecules of ATP and NADH.
GLYCOLYSIS
# Energy Investment Stage:- * Includes reaction steps from one to five. * Glucose to two Glyceraldehyde 3-phosphate. * Two ATPs are invested. * Also called as preparatory stage.# Energy payoff stage:- * Includes reaction steps from six to ten. * Two Glyceraldehyde 3-phosphate to two Pyruvate plus four ATPs. * A net gain of two molecules of ATP and two NADH
molecules.
TWO STAGES:-
A) GLUCOSE
B) ATP
MAIN REACTANTS
A) PYRUVATE
B) ATP
C) NADH
PRODUCTS
1) Hexokinase reaction: phosphorylation of glucose
2) Isomerization of G6P to Fructose-6-phosphate
3) Phosphorylation of F6P to Fructose 1,6-bisphosphate
4) Cleavage of Fructose 1,6-bisphosphate
5) Interconversion of triose phosphate
6) Oxidation of G3P to 1,3-bisphosphoglycerate
7) Phosphoryl transfer from 1,3-bisphosphoglycerate to ADP
to form 3-phosphoglycerate
8) Conversion of 3-phosphoglycerate to 2-
phosphoglycerate
9) Dehydration of 2-phosphoglycerate to phosphoenol pyruvate
10) Transfer of phosphoryl group from phosphoenol pyruvate to ADP
Intermediate step between Glycolysis and Krebs cycle.
Oxidation of Pyruvate
Location:- Mitochondrial matrix. It is an aerobic process. Net zero ATP is produced. Pyruvate molecules transported from
cytoplasm to mitochondria. Converts each pyruvate(3C) into acetyl
coenzyme(2C). 2CO2 released (1from each pyruvate) 2NAD+ reduced to NADH. Reaction:-pyruvate+NAD+CoA Acetyl-CoA+NADH+CO2
(2 pyruvate=2 NADH and CO2)
Details
# Also known as Citric Acid Cycle or TCA(Tri Carboxylic Acid).
# Occurs in mitochondrial matrix.# It is an aerobic process.# Net production of 2 ATPs.# Takes two complete cycles.# Uses Acetyl CoA to generate ATP,NADH,FADH2
and CO2.
# Eight steps, each within an enzyme.
KREB’s CYCLE
REACTION NO. OF ATP/REDUCED COENZYME FORMED
NO. OF ATP ULTIMATELY FORMED
1)Glucose to G6P -1 ATP -1
2)F6P to Fructose1,6-bisphosphate
-1 ATP -1
3)2G3P to 2 1,3-BPG 2NADH 3-5
4)2 1,3BPG to 2,3-PG 2ATP 2
5)2 PEP to 2 pyruvate 2ATP 2
6)2 Pyruvate to 2Acetyl-CoA
2NADH 5
7)2 Isocitrate to 2a-KG
2NADH 5
8)2 a-KG to 2 S- CoA 2NADH 5
9)2S-CoA to 2 Succinate
2ATP(2GTP) 2
10)2S to 2 Fumarate 2FADH2 3
11)2Malate to 2 Oxaloacetate
2NADH 5
TOTAL 30-32
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