global leader of molecular diagnostics & platform ... · panamutyper™r series b tech d tech c...

PANA RealTyper™

· HPV· STD

PANA qPCR™

· EML4-ALK Screening· EML4-ALK Genotyping

PANAMutyper™ R

· EGFR· KRAS· NRAS· BRAF

PNAClamp™

· EGFR· KRAS· NRAS· BRAF· PIK3CA· IDH1· JAK2· BCR-ABL

PNA Oligos

· PNA FISH Telomere Probe· PNA FISH Centromere Probe· miRNA inhibitor· Gamma PNA· Real-time PCR Probe· Globin Redection RNA· Custom PNA

Global Leader of Molecular Diagnostics & Platform Technology Provider

For more information, please visit www.panagene.com 2

PNA is an artificially synthesized nucleic acid.

Phosphate ribose ring of DNA is replaced to polyamide backbone of N-(2-aminoethyl)glycine.

Despite radical structure change, PNA still binds to its complementary DNA or RNA sequences by Watson-Crick base pairing.

PNA provides several physicochemical advantages with stronger binding affinity and chemical stability as probes for molecular diagnostics and antisense drugs.

Criteria PNA DNA

Binding affinity with DNAStronger

(1°C higher per base)

Hybridization rate Faster (100 times)

Salt conc. for hybridization Independent Dependent

ΔTm of single mismatched duplex < 24 °C < 12 °C

Chemical stability Stable Unstable in acid

Water solubilityLimited solubility

: enhanced by linkersSoluble

Probe length for diagnosis 11 - 18 25 - 30

Biological stability Stable to nuclease & protease Enzymatic degradation by nuclease

Thermal stability Excellent Moderate

PNA (Peptide Nucleic Acid)

3|For more information, please visit www.panagene.com 2

PANA qPC

R™

EML4-

ALK

/ PNA O

ligomers

PANA R

ealTyper™PA

NAM

utyper™R Series

PNAC

lamp™

Mutation D

etection Kits

Platform Technologies

Innovative platform technologies in molecular diagnostics area

PNAClamp™

PANA qPCR™ PANA S-melting™

PANA C-melting™ PANArray™

Ct AnalysisMelting-curve

Analysis

selectiveAmplification

MultiplexTarget detection

MultiplexGenotyping

Selective amplification& Multiplex Genotyping Microarray

Somatic Mutation1% detection

Infectious DiseaseDrug Resistance

Infectious DiseasePersonalized Medicine

Somatic Mutation0.01-0.1%

Genotyping &Expression Profiling

EGFR, KRAS, NRAS,

BRAF, IDH1, IDH2,

PIK3CA, JAC2, c-KIT,

BCR-ABL, HER2, ERCC1

TB/NTM,

EML4-ALK,

CRE,

HPV screening

HPV genotyping

STD

RV

CRE

TB/NTM

EGFR, KRAS, NRAS,

BRAF

HPV genotyping, miRNA,

expression, HBV-Drug,

resistant

HCV genotyping

STD,

MTHFR,

CYP450

PNA / Platform

Technologies

Amplification

Mutant DNA

PNA

Foward

Reverse

Infection Disease

Cancer


● PANA C-Melting™Technology

● Features

● ProductsProducts Cat. No. Detectable Mutation Size

EGFR PNAR-3001 Exon 18, 19, 20, 21 (47 mutations) 24 tests

KRAS PNAR-1001 Coon 12, 13, 59, 61, 117, 146 (29 mutations) 24 tests

NRAS PNAR-1101 Coon 12, 13, 59, 61, 117, 146 (31 mutations) 24 tests

BRAF PNAR-2001 V600 (5 mutations) 24 tests

• LOD < 0.1% guaranteed

• Excellent specificity

• Multiplex genotyping

• Relative quantification

• Easy and convenient test procedure

• Powerful analyzer

▶ Validated Specimens - Plasma - Serum - Tissue

▶ Compatible Instruments

- Bio-Rad CFX96 - ABI QuantStudio5

PANAMutyper™R Series

Diagnosis of Cancer for Liquid biopsy

marked

-d(R

FU)/

dT

400

300

200

100

0

-100

40 50 60 70

Temperature, Celsius

Melting Peak

Annealing step

Extension step

Wild-type Allele

Wild-type Allele

Clamping PNA

3'

3'5'

Detection probe

Detection probe

Mutant Allele

Mutant Allele

Mutant Allele

Denaturation

a) b)PCR clamping

MT 10% MT 1%MT 0.1%MT 0.01%MT 0.005%MT 0.001%MT 0%

L858R


● Advantage of PANAMutyper™

New Advanced Mutation Detection Solution for Circulating Tumor DNA

* *

EGFR T790M EGFR L858R KRAS G12D KRAS G12V KRAS Q61R KRAS Q61K

B Tech N Tech d Tech c Tech Q Tech PANAMutyper™ PNAClamp™

5%

2%1%

0.10%

0.01%

Comparison of somatic mutation detection methods

*:Not detected in 5% mutation samples(false negative), §: No test for those mutations

Comparison of somatic mutation detection methods Superiority of PANAMutyper™: The Choice is Clear

PANAM

utyper™R Series

B Tech d Tech c Tech

N Tech Q Tech

Easiness

LOD

Run time Equipment independancy

Assay cost

PANAMutyper™

c Tech

d Tech

B Tech

Increasing Cost Effectiveness

0.01

0.1

1

5Incr

easi

ng T

est Sen

stiv

ity(%

)(M

edia

n L

OD)

General Info : [email protected]

PANA qPC

R™

EML4-

ALK

/ PNA O

ligomers

PANA R

ealTyper™PN

AC

lamp™

Mutation D

etection Kits

PNA / Platform

Technologies


PNAClamp™ Technology is the PNA-based PCR clamping that selectively amplifies only the mutated target DNA sequence as a minor portion in the mixture with the major wild type DNA sequences.

• Compatible with the most popular real-time PCR instruments• Test several biomarkers in one plate - eg.) EGFR+KRAS+BRAF … in one plate• High sensitivity and specificity• Various compatible samples• Mutation detectable with small amount of DNA

Criteria PANAGENE Company A Company B

TechnologyPNAClamp™

& Real-time PCRScorpions™Real-time PCR

& ARMS™Direct sequencing(D-Sequencing)

Sensitivity <1% <1% 15~25%

Procedure Time(except DNA extraction)

2.5 hrs. 3hrs. 11hrs.

Simplicity ++++ ++ +

Reproducibility +++ ++ ++

PNAClamp™Mutation Detection KitsKFDA approved

marked

Real-time PCR-based Ready-to-use Kit● PNAClamp™Technology

● Features

● Panagene’s product vs. other products

PNA annealing

PNA Probe

NO Amplicon

Amplicon

PCR reaction


▶ Validated Specimens

- Tissue (Fresh, FFPE) - Biopsy

- Pleural effusions

- Cytology samples


- Bio-Rad CFX96

- Roche LC480II

- ABI 7500, 7900, StepOnePlus

- Qiagen Rotor-Gene Q

● Products

* Research Use Only

Products Cat. No. Detectable Mutations Size

EGFR PNAC-3002 Exon 18, 19, 20, 21 (40 mutations) 25 tests

KRAS PNAC-1006 Codon 12, 13, 59, 61, 117, 146 (40 Mutations) 25 tests

NRAS PNAC-1101 Codon 12, 13, 59, 61, 117, 146 (43 Mutations) 25 tests

BRAF PNAC-2001 V600 (5 mutations) 50 tests

PIK3CA PNAC-4001 Codon 542, 545 or 546, 1047 (13 mutations) 25 tests

IDH1 PNAC-5001 R132 (5 mutations) 25 tests

JAK2 PNAC-6001 V617F 25 tests

BCR-ABL* PNAC-7001 T315I 25 tests

Technical Question : [email protected]

PNAC

lamp™

Mutation D

etection Kits

PANA qPC

R™

EML4-

ALK

/ PNA O

ligomers

PANA R

ealTyper™PA

NAM

utyper™R Series

PNA / Platform

Technologies


• Tm analysis for high multiplexing in real - time• Specific PNA probe for target HPV, STD and RV

Products Cat. No. Detectable Mutation Size

HPV PNAM-1001 40 HPV types (genotyping 22 types, screening 18 types) 48 tests

STD PNAM-2001 13 STD types (NG, CT, UU, UP, TV, MG, MH, CA, HD, T.P, HSV1, HSV2, GV) 48 tests

PANA RealTyper™marked

● PANA S-Melting™Technology

● Features• High sensitivity and specificity• High reproducibility• Multiplex genotyping

• Easy and convenient test procedure• Powerful analyzer

● Products

DenaturationExtension

Annealing

PNA Probe

PNA Probe

-d(R

FU)/

dT

500

400

300

200

100

0

40 50 60 70 80 90


Melting Peak

-d(R

FU)/

dT

1200

1000

800

600

400

200

040 50 60 70 80 90


Melting Peak

-d(R

FU)/

dT

500

400

300

200

100

040 50 60 70 80 90


Melting Peak

-d(R

FU)/

dT

1000

800

600

400

200

040 50 60 70 80 90


Melting Peak

PNA Probe(HEX)

PNA Probe(FAM)

PNA Probe(ROX)

PNA Probe(Cy5)

▶ Validated Specimens

- Cervical swab sample - Liquid based cytology sample


- Bio-Rad CFX96 - ABI QuantStudio5

PCR Amplification Melting Curve


◆ Tube A : NG, CT, UU, UP, TV, MG, MH◆ Tube B : CA, HD, T.P, HSV1, HSV2, GV

Real-time PCR based Multiplex Genotyping Kit

● PANA RealTyper™HPV

◆ Tube A and B : High Risk Type and Low Risk Type - 20 High Risk HPV Genotypes:

16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 70, 73, 82- 2 Low Risk HPV Genotypes:

6, 11

◆ Tube O : Other Types : Screening 18 Types- 18 HPV types: 32, 34, 40, 42, 43, 44, 54, 55, 62, 81, 83, 30, 61,67, 74, 84, 87, 90

● Comparison with Current HPV Testing Methods

● PANA RealTyper™STDPANA RealTyper™ STD Genotyping Kit takes advantages of superior PNA properties to DNA. STD genotyping can be completed in a shorter time with higher sensitivity and specificity. Single or multiple infections among 13 STD can be accurately identified.

UP+MG TV+MG

-d(R

FU)/d

T

400

300

200

100

0

40 50 60 70 80


Melting Peak

UP+TV+MG

-d(R

FU)/d

T

400

300

200

100

0

40 50 60 70 80


Melting Peak

-d(R

FU)/d

T

500

400

300

200

100

0

40 50 60 70 80


Melting Peak

Testing Method Provided Information

Genotyping Co-infection Turn around time

Hybrid capture II X X 3 hours

Microarray /bead ○ ○ Over 5 hours

DNA sequencing ○ X Over 8 hours

Real-time PCR ▲ ▲ 3 hours

PANA RealTyper™ ○ ○ 3 hours

○ : Provided / ▲ Limited provided / X : Not provided

Quote Request : [email protected]

PNAC

lamp™

Mutation D

etection Kits

PANA qPC

R™

EML4-

ALK

/ PNA O

ligomers

PANA R

ealTyper™PA

NAM

utyper™R Series

PNA / Platform

Technologies


PANA qPCR™ technology uses a sequence-specific PNA probe with a fluorescent reporter and quencher. The PNA probe is designed to hybridize specifically to a nucleotide region of the target sequence. PNA probe is not hydrolyzed during amplification because its resistant to enzyme degradation. PANA qPCR™ is a powerful tool for multiplexing, allelic discrimination experiments and quantitative analysis with high specificity and sensitivity.

Products Cat. No. Detectable Variants Size

Screening PNAQ-3101 25 variants (V1, V6, V3a, V3b, V2, V4, V7, Vnew, V5a, V5b, V8a, V8b, ect) 24 tests

Genotyping PNAQ-3201 12 variants (V1, V6, V3a, V3b, V2, V4, V7, Vnew, V5a, V5b, V8a, V8b) 10 tests

PANA qPCR™EML4-ALK Screening Kit PANA qPCR™EML4-ALK Genotyping Kit

Components 1 tube ( EML4-ALK fusion gene + Internal control )

4 tube (12 EML4-ALK fusion gene + Internal control )

Coverage 25 EML4-ALK fusion variants detectable 12 EML4-ALK fusion variants detectableDye ROX, HEX ROX, HEX, FAM, CY5

Probe Common Probe Specific ProbeSensitivity 10^2 copy 10^2 copy

Assessment EML4-ALK positive or negative Assess each variantSize 24 tests / kit 10 tests / kit

PANA qPCR™ EML4-ALK™RUO (Research Use Only)

● PANA qPCR™Technology

● Products

● Features

▶ Validated Specimens - Tissue (Fresh, FFPE)- Biopsy- Pleural essusions- Cytology samples


- Bio-Rad CFX96- Roche LL480Ⅱ- ABI7500, 7900, StepOnePlus- Qiagen Rotor-GeneQ

Common probes

Specific probes

Quenched status

Hybridized status

EML4 ALK

F : fluorophoreQ : quencher

F

F

Q

Q

template DNA

Screening

Genotyping

PNA probe


PNA-DNA hybridization is much faster than DNA-DNA and has shown much higher signal to background noise ratio.

PANAGENE has developed PNA based miRNA Inhibitors with higher sensitivity, reproducibility and stability in the cell. We provide all types of PNAs™ miRNA inhibitors to inhibit miRNA available from miRBase Sequence Database hosted by the Sanger Institute (http://microrna.sanger.ac.uk).

Reference1.Su Young Oh et al., Oligonucleotides. 2010 Oct; 20(5):225-30 2. Fabani MM et al., Nucleic Acids Res 1-10.

Panagene has developed PNA based miRNA Inhibitors with higher sensitivity,reproducibility and stability in the cell. We provide all types of PNAs™ miRNA inhibitors to inhibit miRNAs available from miRBase Sequence Database hosted by the Sanger Institute (http://microrna.sanger.ac.uk).

For more information, please visit www.panagene.com

Telomere probe Centromere probe

- Telomere, Centromere probes- Q-FISH, CO-FISH, Flow-FISH - Fast hybridization- Custom probe design possible

- Unlabeled PNA - Labeled PNAs with dyes, biotin or other functional groups- Modified PNAs for your various research - Special PNAs from unnatural bases - High purity (up to 99%) - Large scale manufacturing - PNA monomers- Bis-PNAs- Gamma PNAs(γPNA)

* right-handed helix* enhanced PNA-DNA stability* more handle for labeling* improved solubility* stereogenic center

PNA Oligomers

● FISH probes

● miRNA Inhibitors

● Custom PNA Oligomers

PNAC

lamp™

Mutation D

etection Kits

PANA qPC

R™

EML4-

ALK

/ PNA O

ligomers

PANA R

ealTyper™PA

NAM

utyper™R Series

PNA / Platform

Technologies

Products List

Products Cat. No. Details Size

PANAMutyper™ R Series (for Plasma)

EGFR PNAR-3001 Exon 18, 19, 20, 21 (47 mutations) 24 tests

KRAS PNAR-1001 Coon 12, 13, 59, 61, 117, 146 (29 mutations) 24 tests

NRAS PNAR-1101 Coon 12, 13, 59, 61, 117, 146 (31 mutations) 24 tests

BRAF PNAR-2001 V600 (5 mutations) 24 tests

PNAClamp™ Mutation Detection Kit (for Tissue)

EGFR PNAC-3002 Exon 18, 19, 20, 21 (40 mutations) 25 tests

KRAS PNAC-1006 Codon 12, 13, 59, 61. 117, 146 (40 mutations) 25 tests

NRAS PNAC-1101 Codon 12, 13, 59, 61. 117, 146 (43 mutations) 25 tests

BRAF PNAC-2001 V600 (5 mutations) 50 tests

PIK3CA PNAC-4001 Codon 542, 545 or 546, 1047 (13 mutations) 25 tests

IDH1 PNAC-5001 R132 (5 mutations) 25 tests

JAK2 PNAC-6001 V617F 25 tests

BCR-ABL PNAC-7001 T315I 25 tests

PANA qPCR™ EML4-ALK Detection Kit

Screening PNAQ-310125 variants

(V1, V6, V3a, V3b, V2, V4, V7, Vnew, V5a, V5b, V8a, V8b, ect.)24 tests

Genotyping PNAQ-320112 variants

(V1, V6, V3a, V3b, V2, V4, V7, Vnew, V5a, V5b, V8a, V8b)10 tests

PANA RealTyper™Kit

Products Cat. No. Details Size

HPV PNAM-1001 40 HPV types (genotyping 22 types, screening 18 types) 48 tests

STD PNAM-200113 STD types

(NG, CT, UU, UP, TV, MG, MH, CA, HD, T.P, HSV1, HSV2, GV)48 tests

Cancer

Infectious Disease

Distributed by Eurogentec Website : www.eurogentec.com Contact: [email protected]

global leader of molecular diagnostics & platform ... · panamutyper™r series b tech d tech c...

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