GHS ScienceCAPT Embedded Task Review

Lab Investigation: Enzymes

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Your Task

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Lab Design

1. Place filter paper in a funnel and put funnel in a flask.

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Funnel with filter paper in it

Flask

Most GHS students used a procedure that looked something like this:

Lab Design

2. Set up three flasks with funnels.

5 A B C

Lab Design

3. Pour exactly 20 g of apple sauce into each funnel.

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20 g of apple sauce

20 g of apple sauce

20 g of apple sauce

A B C

Lab Design

4. Add a different enzyme to each funnel.

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8 ml of Pectinase

8 ml of Cellulase

No enzyme added

A B C

Lab Design

5. Let each flask sit for exactly 10 minutes.

8 A B C

Lab Design

6. After 10 minutes, measure how much juice was produced from each flask using a graduated cylinder.

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Graduated Cylinder

A B C

Apple Juice Produced

Apple Juice Produced

Apple Juice Produced

Sample DataEnzyme(s)

Flask APectinase

Flask BCellulase

Flask CNo Enzyme

AddedAmount of

Juice Produced3.0 ml 5.0 ml 0.5 ml

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Analysis Questions

Directions: On a separate sheet of paper, answer the following questions in complete sentences.

1. Identify the following for this experiment:

a. Problem statementb. Independent variablec. Dependent variable

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Analysis Questions

2. Identify two variables that were held constant in this

experiment.

3. Explain why it is important to hold these variables constant.

4. What was the substrate and the product(s) in this experiment?

5. Why was it important that “flask C” was used?

6. Based on the data, what would your conclusion be for this

experiment?13

Going Further… Challenge Questions, Set 1

7. After performing this experiment, you learn from the apple juice

company that Pectinase and Cellulase cost different amounts:

a. Pectinase: $.50 per ml

b. Cellulase: $1.00 per ml

The apple juice company wants you to be as cost-effective as

possible in producing the most amount of juice.

Explain to the company which enzyme is most cost-effective (ml/$)?

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Going Further… Challenge Questions, Set 2

A group of students are convinced that pectinase is the most cost-

effective enzyme to choose to produce apple juice for the company.

They think that temperature may affect how much juice pectinase can

produce.

In a new experiment, they test their hypothesis. Using the same basic

design as the original experiment, the students heat apple sauce with

pectinase to different temperatures to see which temperature is

optimal for producing the most apple juice.

The temperatures are: 0°C, 20°C, 40°C, 60°C, 80°C16

Going Further… Challenge Questions, Set 2

The results of the students’ new experiment are shown below:

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0 20 40 60 80Degrees Celcius

Going Further… Challenge Questions, Set 2

8. Explain the behavior of pectinase in temperature range 0-20 °C.

9. Explain the behavior of pectinase in temperature range 50-80 °C.

10. What would be an appropriate conclusion for the new experiment?

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