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Pennsylvania Highlands Community College
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Fall 2012
Pennsylvania Highlands Community College
Index
Welcome…………………………………………………………………………..1
Frequently Asked Questions …………………………………………………….2
Pennsylvania Highlands Community College
Welcome
Quick Contact List for New Faculty
Name Title Contact
William Wolff Assistant Professor, Chemistry
262-6479
Jill Mitchell Instructor, Biological Sciences Lead Faculty
262-6487
Ashley Hollern Instructor, Biological Sciences 262-6412
We want to first welcome you to the Biology Department, here at Pennsylvania Highlands Community College. This packet includes information that will be useful in your faculty role as an instructor. This is designed to make your transition into the Biology Department at Pennsylvania Highlands Community College more pleasant and productive. The Biology department provides a solid foundation in science by instilling in students a commitment to search for truth through application of the scientific method, to gain a better understanding of humans, the earth and the universe, and to desire knowledge that can be applied to the service of others. The department offers courses that fulfill the college's Core Curriculum, as well as courses that support the Biotechnology Program. Your role as a member of the department is to aid our endeavors of creating a learning environment that enhances critical thinking, allows for collaboration, and exploration through experience which lends to life-long learning skills.
WELCOME TO PENNSYLVANIA HIGHLANDS
COMMUNITY COLLEGE BIOLOGY DEPARTMENT
Summer 2011
Volume 1,
Issue 1
Important Phone Extensions
Security – x 6247 Security Emergency – x 5555 IT Help Desk – x 6470 Front Desk – x 6400
To make a local call from any room
dial 9 first.
101 Community College Way, Johnstown PA 15904
www.pennhighlands.edu 814-262-6400
Wolff, William, Associate Professor, Natural Sciences and Technology Mitchell, Jill, Instructor, Biological Sciences Hollern, Ashley, Instructor, Biological Sciences
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1. How do I obtain the keys for my classroom?
The keys for your lecture are lab room can be obtained from Mary Ann Lee during the
hours of 8:30am and 4:30pm. If your class is held after these hours you should contact
security at x6427 which can be dialed from any open classroom or from the faculty copy
room.
2. Who are the full time departmental employees in biology?
Jill Mitchell is the Biology Department lead faculty. She teaches BIO110, and BIO115.
Her office is located in C. Ashley Hollern is a full time biology faculty member and
teaches BIO210, BIO260, and BIO262. Her office is located in C
3. Who can I ask for assistance while teaching lecture / lab?
Feel free to contact either Jill Mitchell at x 6487 or Ashley Hollern at x 6412 with any
questions or concerns regarding lab or lecture questions.
4. What supplies are available to me?
You will find a list of supplies available to you in this manual. See pages ****
5. Is it ok to borrow supplies from another lab cabinet?
We would prefer you obtain the lab supplies from the lab cabinet assigned to your class.
However, if supplies are in short supply and you must borrow supplies from another
cabinet, please make a note of the need for a purchase order. There is a tablet for
“supplies needed” that can be found in C230B Storage room, or C226 B Storage room.
6. Where do I make copies?
Copies can be made in the faculty copy room located in C*****
7. Who do I contact if I need additional supplies?
If you are aware of the need for additional supplies at the beginning of the semester, you
should contact Jill Mitchell with the Supply list, company name and purchase order
information so that the order may be processed in a timely manner.
8. How do I address supplies that I run out of during the semester?
If you should run out of supplies during the semester and no replacements are available,
contact Jill Mitchell ASAP so that an order may be processed.
9. What is the proper PHCC procedure for disposing of laboratory material?
You will find a “Lab Safety” section in the manual outlining the proper procedure of
handling hazardous materials handled during the semester.
10. Where can I find manuals to the lab equipment available to me?
The lab equipment manuals can be found in the C230 A Prep Room, located in the
drawer beneath the incubators labeled “Equipment Manuals”
11. How do I address Lab Safety in Lab?
At the start of the semester, students should be made aware of proper lab safety protocol.
You are required to show a brief video on “Lab Safety” from Flinn Scientific. Following
the video ALL students are to receive the “Lab Safety Contract” that they must read and
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sign, prior to the start of any lab. Please keep the contracts until the end of the semester,
at which time you must turn them into Jill Mitchell.
12. The lab stations are out of lab disinfectant, where can I find more?
The lab countertop disinfectant can be found in the C230 prep room to the left of the
dishwasher in a cabinet marked “Lab Disinfectant”
13. Where the emergency gas shut-off located in the lab rooms?
The emergency gas shut off is a large red button located near the door. By pushing the
button in, the gas is shut “off”.
14. What is the recharging procedure for the chargeable microscopes located in C224/
C226? Microscopes that have been used should be plugged into the outlet strip and the timer
knob turned. The timer will turn the charge off automatically after 8 hours. Microscopes
that were not used should not be plugged in during this process. Overcharging of the
scopes will shorten the life of the battery.
15. Usage of the dishwasher for glassware? What is it primarily used to clean? It can be
used to clean glassware, but it is best to have students wash glassware at the end of lab so
that glassware remains available to all labs and isn't hanging out in the dishwasher.
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Welcome to The Microbiology Lab C230
The lab and lecture rooms on campus are used frequently
for both day and evening classes. Therefore it is of utmost
importance that the materials used during class and lecture
be returned. ALL supplies and equipment should be put
away after use and all glassware needs to be cleaned.
You are permitted to prep your materials ahead of time and
place the materials on a marked cart in the prep rooms of
the lab. However following the lab sections the materials are
to be put away.
Also, it is your responsibility as an instructor to prep your
own labs. As the instructor, you are to plan accordingly to
make sure all the materials you need are available prior to
the start of lab.
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The following is a list of stocked items, and equipment available for each of the biology
courses. If you require additional supplies not present on the list, please contact Jill
Mitchell with your order ASAP to assure your purchases are made. In your order supply
the company name, item name, item number and quantity required.
Note: Not all additional orders are guaranteed and are based on funds available at the time.
BIO 110 Principles of Biology Storage Room C230B
Sugar
Glycerin
Dish detergent
Straws
pH paper
antacid tablets
3 beverages: i.e. soda, iced tea,
Gatorade, etc.
Milk
String
Pennies
Ethanol
Vegetable oil
Lemon juice
Ammonia
Benedicts solution
Iodine solution Buiret solution
Sudan IV solution
Starch solution
Glucose solution
Egg white
Elodea
Agar
Corn syrup
Syringes
Potato cores
Dialysis tubing
Well plates
Sodium bicarbonate
Food coloring
Yeast
Balloons
Bromothyml blue
Sucrose
Prepared mitosis slides
Magnetic chromosome kits
Electrophoresis gels, etc.
Evolutionary agents kit
BIO 115 Principles of Biology II Storage Room C230B
BIO 210 Microbiology Storage Room C230B
Microscopes
Staining trays
Test Tube Racks
Gloves
Prepared Slides
o Letter “e” slide
o Bacterial Shapes
o Gram Stain
Glass Slides
Cover Slips
Rubber Bulb Pippets
Toothpicks
Oil
Stains / Alcohol
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o Methylene blue
o Crystal violet
o Safranin
o Nigrosin
o Safranin
o Methyl red
o Iodine
o Ethyl alcohol
o Acid fast alcohol
o Carbol fuchsin
o Methyl green
o
Pond Water
Sterile Swabs
Bibulous paper
Kim wipes
Petri plates
Test tubes
Sterile Cotton Swabs
Inoculating Loop
Bunsen Burners
Hot Plates
Clothespins
Wax marking pencils
Alcohol Pads
Specimen (All specimen are located in
cooler unless otherwise noted)
o Escherichia coli
o Staphlococcus aureus
o Streptococcus pyogenes
o Clostridium botulinum
o Bacillus megaterium
o Corynebacterium diphtheriae
o Micrococcus luteus
o Enterobacter aerogenes
o Serretia marcescens
o Mycobacterium smegmatis
o Saccharomyces cerevisiae
What’s in your water Kit
Agar:
o Nutrient
o Eosin Methelyne Blue
o Sabouraud agar
o MacKonkey agar
o Trypticase Soy agar
o Nutrient broth
BIO 260 Anatomy and Physiology I Storage Room C226B
Microscopes
Dissecting Kits
Gloves
Synthesizing Macromolecules lab
Set
Dialysis Tubing
Iodine
Starch
Onion Root Tip / Ascaris Slides
Tissue Slides
Bones (2 boxes- green, blue)
Skeletons (4)
Muscles (see models below)
Scapels
Mammal Spinal Cord
Mammal Brain
Mammal Eye
Human Senses Lab Kit
Phenyl-thiocarbamide (PTC)
Thiourea
Sodium Benzoate
BIO 262 Anatomy and Physiology II Storage Room C230B
Microscopes
Gloves
Dissecting Kits
Blood Group Identification Lab
Mammal Heart
Double Injected Cats
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Blood Pressure Cuffs
Stethoscopes
Models:
- (2) Human Leg Muscle
- (2) Human Arm Muscule
- (2) Human Torso
- (3) Atlay Human Heart
- (3) Human Lung
- Kidney Model (Small)
- Cross Section With Description
chart
- Kidney Model (Med)
- Cross Section on stand
- Kidney Model (Large)
- Cross Section with description
booklet in base.
- Cell Model Globe
- Liver Model (Large) Cross
Section
- Reflex Arc Model (Large, Flat)
- Eye Model (small) Eyeball only
- Eye Model (Medium) in socket
with muscle and bone sites
- Eye Model (Large) in socket
with muscle and bone sites
- (5) Brain Model (small)
- external colored regions ,and
cross section marked sites
- (4) marked sites cards for
identification purposes
- Male Reproductive Organs in
box
- Brain Model (Large)
- External and Cross Section with
description booklet in base
- Brain Model in Head (Small)
LAB Equipment Prep / Storage Room C230A/B
- Microscopes
- Balances
- Hot Plates
- Stirrers
- Water Distiller
- Bunsen Burners
- Microscopes
- Autoclave
- Incubators (2)
- Fume Hood (2)
- Dishwasher
- Cooler
- Refrigerator
- Gel Electrophoresis Chambers
- Micropippeters
- Thermometers
- Glassware
- Test Tube Racks
Note: The lab equipment manuals can be found in the C230 A Prep Room in a
cabinet drawer labeled, “Equipment Manuals”.
LAB Equipment C226 Prep / Storage Room C226A/B
- Beakers
- Microscopes
- Yard Sticks
- Cooler
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Index
A. OSHA Laboratory Safety Guidance
B. Disposal of Biological Specimen
C. OSHA Bloodborne Pathogens and Hazard Communication Standards
A. OSHA Laboratory Safety Guidance
Here are a list of helpful links to documents provided by the Occupational Safety and Health
Administration.
Laboratory Safety Guidance
OSHA Fact Sheet OSHA’s Bloodborne Pathogens Standard
OSHA Fact Sheet Chemical Hygiene Plan
OSHA Fact Sheet Laboratory Safety OSHA Laboratory Standard
OSHA Quick Facts Laboratory Safety Autoclaves / Sterilizers
OSHA Quick Facts Laboratory Safety Labeling and transferring of Chemicals
Other Helpful Links:
Material Safety Data Sheets
Center for Disease Control and BIO Safety
World Health Organization
Management and Disposal of Biological Waste
At Pennsylvania Highlands Community College
I. Introduction
The purpose of this document is to provide information, requirements, guidelines and
procedures for the handling and disposal of hazardous and non-hazardous biological
waste for all departments and units on the Pennsylvania Highlands Community
Pennsylvania Highlands Community College
College campus. Currently there are three departments or units that generate
biowaste: Science, Healthcare Management, and Workforce Education.
“Biological Waste” or “Biowaste” means discarded biological material from teaching
and clinical laboratories and operations. This does not include household or office
trash.
“Biohazardous Waste” means any solid or liquid biological waste that is hazardous
because of its physical and/or biological nature. All waste that contains infectious
material, or because of its biological nature, may be harmful to humans, animals,
plants, or the environment is biohazardous waste. This includes waste from
infectious animals, human blood or blood products, pathological waste, and sharps.
II. Responsibility
Faculty member or other person with operational responsibility
1. Shall be responsible for ensuring students in classes generating biohazard waste
comply with the provisions of the plan.
2. Shall take appropriate steps to assure the safety of the students involved in the
handling of biowaste.
3. Will take immediate decontamination measures, primarily consisting of intensive
flushing, and other preliminary first aid procedures in the event of a spill or other
accident, while notifying the Director of Security and Safety or his employees
who will in turn notify appropriate medical and HAZMAT first responders.
Science lab manager, Health Care Management appointee, Director of
Workforce Education
1. Shall be responsible for the proper storage, handling and packaging for disposal
of biological waste generated from their respective programs. Material for
disposal shall be clearly marked in the red biohazard bags provided, and stored in
the “prep” rooms, room 224A for Healthcare Mgmt. and Workforce Ed., as well
as in room 230 for Science, pending pickup. Boxes should also be clearly marked
with the name of the originating department for billing purposes. Access to these
locked rooms shall be strictly limited to appropriate faculty, or to students under
direct supervision of those faculty members, and all access doors will be clearly
marked with signs depicting the universal biohazard symbol.
2. Shall notify the Director of Security and Safety when a sufficient number of
containers of biowaste or sharps are ready for disposal (2-3 standard boxes) so
that a pickup can be scheduled. Will also place orders for replacement containers
at this time.
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3. Shall immediately report spills or other events to the Office of Security and
Safety, while ensuring that any necessary decontamination and first aid to affected
persons is immediately performed. The Office of Security will respond
immediately to the scene of the incident and will coordinate immediate
deployment of medical and HAZMAT first responders as appropriate.
Facilities Manager
1. Shall be responsible for receiving requested replacement biowaste supplies from
the disposal company at the time of a scheduled pick-up and distributing the same
to the various departments.
2. Shall be responsible for directing invoices and other paperwork resulting from a
pickup to the Director of Security and Safety, who will in turn file or distribute to
the appropriate departments as necessary.
3. Shall be responsible for transporting containers of biowaste from the “prep” room
to a holding area. This includes assuring that the containers are secure while they
await removal by the disposal company. Containers of “sharps” will be
transferred to the holding area approximately one hour prior to the anticipated
arrival of the disposal company.
4. Facilities Manager will oversee the actual loading and removal of biowaste.
Director of Security and Safety
1. Shall assume primary oversight responsibility for procedures as outlined in this
plan, as well as ensuring that they conform to applicable state and federal
regulations.
2. Shall be notified immediately of any incident occurring on PHCC property
involving biowaste spills or other related accidents, particularly those which may
result in the contamination of students, faculty, staff or others. Will coordinate
notification to appropriate medical and HAZMAT first responders, and will
proceed immediately to the accident scene to assist with decontamination and first
aid, as well as the evacuation of personnel and securing the area. Subsequent
notification of the incident will also be made to the PA Department of
Environmental Protection.
3. Following the incident will complete a PHCC Incident/Injury report, and will
convene an after-action meeting attended by all involved parties for the purpose
of identifying the cause(s) of the accident, as well as making recommendations
aimed at avoiding a reoccurrence.
4. Will ensure that all required records and notifications to regulatory agencies are
being accomplished correctly and in a timely fashion. Will maintain a file of
Department of Environmental Protection (DEP) forms # 2540, Infectious and
Chemotherapeutic Manifest, documenting pickup and eventual disposal (i.e. chain
of custody).
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5. Upon notification by the referenced departments that a pickup is required, shall
contact the disposal company and make arrangements, and that information will
be relayed to the departments and to the Facilities Manager. Will also relay
orders for replacement biowaste containers as requested from the departments.
III. Segregation
1. Any waste that could produce lacerations or puncture injuries must be disposed
of as “sharps”. Sharps must be segregated from other waste. Metal sharps and
broken glass that pose a biological hazard may be comingled with each other, but
not with other waste. Sharps should not be comingled with regular laboratory
glassware which has been broken and disposed of in an appropriate and separate
container.
2. Biological waste must not be comingled with chemical waste or other laboratory
trash.
3. Biohazardous waste should be separated from other biological waste.
IV. Containers
Containers must be appropriate for the contents; not leak; be properly labeled; and
maintain their integrity if thermal or chemical treatment is used. Containers of
biohazardous materials must be kept closed.
1. Sharps—use a rigid, puncture-resistant container (heavy-walled plastic is
recommended) for storage and disposal. These are usually provided by the
disposal company.
2. Solid Biohazardous Waste—Use heavy- duty plastic “Biohazard Bags” (autoclave
bags) or containers for biohazardous waste.
3. Solid Non-hazardous Waste—Use heavy-duty plastic bag or other appropriate
container without a Biohazard symbol.
V. Storage
Biological waste may be held temporarily under refrigeration, prior to disposal, in a
safe manner that does not create aesthetic problems. Biohazardous waste should be
treated and disposed of promptly. Containers holding biohazardous material must be
clearly labeled, including the biohazard symbol.
Storage of any type of biological waste, as well as any other disposal materials, for
the various departments are as follows:
Science—the storage or prep room adjacent to Rm C230
Healthcare Management—the smaller storage room off of the large prep room
between Rms. C224 and C226.
Workforce Education—the large prep room between Rms. C224 and C226.
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VI. Procedures for Treatment and Disposal
1. Microbiological Waste
a.) Should be treated (disinfected) promptly on-site by autoclaving at 15 psi and
at least 121 °C for at least 30 minutes. Autoclaving is the preferred
disinfection method. However, if autoclaving is not possible, microbiological
waste may be chemically disinfected by immersion, for not less than three
minutes, in a 1:10 (volume/volume) bleach solution.
b.) Once treated as above, the waste may be deposited in the regular trash as long
as the autoclave bag has been labeled “treated” and then placed in another bag
that is opaque—but not a biohazard bag.
2. Animal Carcasses and Body Parts
The dissection specimens that the college purchases may be disposed of
by way of the regular trash according to the manufacturer. However, it is current
policy to dispose of preserved specimens via a disposal company. Therefore,
these carcasses and body parts should be handled as follows:
a.) During the semester or session, the specimens should be double-bagged, to
prevent leakage, and stored in the refrigerator nearest the lab where the
dissection is being performed.
b.) At the end of the semester, the double-bagged specimens should be placed
into a cardboard container, supplied by the disposal company, that is lined
with a red biowaste bag. Care should be exercised to not exceed the weight
limit of the box, which is typically 50 lbs. In all cases the packaging
instructions of the disposal company should be followed (See attached).
c.) Notify the Facilities Manager when the package is ready for disposal.
3. Sharps
a.) Sharps should be placed into an approved sharps container immediately after
use. The container should be kept closed between uses.
b.) At the end of the semester or when the sharps container is full, the container
should be placed into a cardboard container, supplied by the disposal
company, that is lined with a red biowaste bag. In all cases the packaging
instructions of the disposal company should be followed (See attached).
c.) Notify the Director of Security and Safety when the package is ready for
disposal.
WEW/9-25-2009
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