GENUITY® Corn on Corn

Corn Rootworm Update

July 31, 2012 Spencer, IA

Craig Lamoureux Monsanto Technology Development Rep

Corn Rootworm Update

Welcome

•Node Injury Scale (NIS) 0-3 and the definition of CRW damage

•Refresher on corn rootworm biology – life cycle

•Monsanto Corn rootworm technology history/background

•2011 Location Data

•2012 recommendations

0.01 1.0 2.0 3.0

0-3 Iowa State Node-Injury Scale (Oleson 1998)

Less Damage Greater Damage

Iowa State Node-Injury Scale

2nd

Larval Stage (4-9 mm long)

3rd Larval Stage (10-16 mm long)

Pupa – 1 to 2 weeks

WCR Adult – emerge

July and early August

Eggs – Laid in soil

from late July to October

and overwinter in soil

Rootworm Life Cycle And Biology

1st

Larval Stage (1-3 mm long) –

emerge in late May/early June) WCR & NCR

Life Cycle

1 generation per year

CRW Life Cycle

Source, S. Wagner, M. Zellner, LFL; JKI

Growing season Planting Harvest

2003 EPA Grants Approval of YGRW and YGPL

in February and October respectively

• Monsanto’s portfolio of corn-rootworm products continue to provide

outstanding performance and grower satisfaction across tens of

millions of acres. In nearly all of these areas, our recommendations

remain the same.

2003 Treatment

Comparisons

0-3 Iowa State Node-Injury

Scale Below (Oleson 1998)

0.01 1.0 2.0 3.0

Less Damage Greater Damage

Background • From the time that we first introduced YieldGard® Corn Rootworm

technologies, Monsanto has been responding to occasional reports of

greater than expected corn rootworm damage to our single-gene CRW

products, with the majority in limited portions of a few states in the

corn-growing area.

2006

NIS = 2.25 RR2/YGPL vs

NIS = 0.75 RR2+Aztec

C-C Cylinder, IA

Background • In 2011, we again worked with farmers to better understand greater

than expected damage in limited geographies with high corn rootworm

populations, and based on that work, we are providing management

recommendations for those areas.

Refuge with Insecticide Genuity® SmartStax®

What factors could cause variability in performance?

Hybrid

Overwinter CRW Survival

2010-2011 Problem Field Studies

ISU 2011 Node Injury Scores by corn traits

2 Locs Northeastern Iowa problem fields (2010)

HXX

VT3

VT3 VT3 SS

2011 ISU Studies

ISU 2011 Western CRW Adult Survival by corn trait

2 Locs Northeastern Iowa problem fields (2010)

VT3

VT3

VT3

HXX SS

2011 ISU Studies

Monsanto Academic Trials at Nashua

and Hopkinton

• 96RM Isoline for all RR2, VT3, and SmartSTAX

• Not part of the protocol, a 105RM Herculex Xtra from

Mycogen

• Shows Node Injury Rating on a Herculex trait when grower

had never planted Herculex Xtra before

1.90

0.30

0.57

2.50

0.33

1.23

0.37

1.25

1.40

0.32

0.43

2.13

0.62 0.64

0.11

0.53

0.00

0.50

1.00

1.50

2.00

2.50

3.00

VT3 VT3/Aztec VT3/Force RR2 RR2/Aztec RR2/Force Smartstax HXX

Hopkinton Nashua

Node Injury Scale (0-3)

57

11

1

100

3 7 6

17

3 0 0

29

0 0 0 0 0

20

40

60

80

100

120

VT3 VT3/Aztec VT3/Force RR2 RR2/Aztec RR2/Force Smartstax HXX

Hopkinton Nashua

Percent Lodged Plants

188

223 225

195

231

203

220 221

203 201 210

185 182

201 208

231

0

50

100

150

200

250

VT3 VT3/Aztec VT3/Force RR2 RR2/Aztec RR2/Force Smartstax HXX

Hopkinton Nashua

YIELD in Bushels per Acre

Hopkinton Yield vs NIS

188

223 225 195

231

203

220 221

1.90

0.30

0.57

2.50

0.33

1.23

0.37

1.25

0.00

0.50

1.00

1.50

2.00

2.50

3.00

0

50

100

150

200

Yield NIR HXX-105RM, Rest-96RM

Hopkinton Location NIS

2.50

1.90

1.25

0.37

0.00

0.50

1.00

1.50

2.00

2.50

3.00

RR2 VT3 HXX Smartstax

Nashua Yield vs NIS

203 201 210

185 182

201 208

231

1.40

0.32 0.43

2.13

0.62 0.64

0.11

0.53

0.00

0.50

1.00

1.50

2.00

2.50

3.00

0

50

100

150

200

250

VT3 VT3/Aztec VT3/Force RR2 RR2/AztecRR2/Force Smartstax HXX

Yield NIR HXX-105RM, Rest-96RM

Other 2010 Problem Fields with 2011 data

TRUMM Farms NIS with Identified Thresholds

0 0 0.06 0.117 0.008 0.018

0.001 0.001 0.008 0.123 0.005 0.001 0

0.25

0.5

0.75

1

1.25

1.5

1.75

2

2.25

2.5

2.75

3

GENSS /W Ins VT3 /W Ins RR2 /W Ins RR2 VT3 GENSS

NIR Rep 1 NIR Rep 2

TRUMM Farms % Root Lodging

3

10

30

70

30

10

3

10

20

35

15

10

0

10

20

30

40

50

60

70

80

GENSS /WIns

VT3 /W Ins RR2 /W Ins RR2 VT3 GENSS

Root Ldg Rep 1 Root Ldg Rep 2

TRUMM Farms Yield

224.7 227.2 232.7

228.5 230.1 221 223.2 223

233.9 231.2 224.9

220.9

100

120

140

160

180

200

220

240

260

GENSS /WIns

VT3 /W Ins RR2 /W Ins RR2 VT3 GENSS

Yield Rep 1 Yield Rep 2

Lockhard Farms NIS w/REP Average & Thresholds

0.536

0.108

0.538

0.047 0.008

0.19

0.046

0.857

0.1

0.002 0.008 0.008

0.885

0.093 0.035

0.245

0.054

0.760

0.080

0.015

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

VT3 VT3/W Ins RR2 RR2/W Ins GENSS

NIR Rep 1

NIR Rep 2

NIR Rep 3

NIR Average

.25

Lockhard Farms Yield

190 192

185

200

212

183

193 190

197

206

188

180

188

194

203

160

170

180

190

200

210

220

VT3 VT3 W/Ins RR2 RR2 w/insect. GENSS

Rep1 Rep 2 Rep 3

HOSCH Farms NIS with Thresholds

0.008 0.007 0.008 0.116 0.129

0.002 0.013 0.15

0.248

0.476

0.075 0.001

0.007 0.007 0.058 0.113 0.115

0.015 0

0.25

0.5

0.75

1

1.25

1.5

1.75

2

2.25

2.5

2.75

3

GENSS/W Ins VT3/W Ins RR2/W Ins RR2 VT3 GENSS

NIR Rep 1 NIR Rep 2 NIR Rep 3

HOSCH Farms Yield

218.8 217

199.9 191.4

199.3 193.5

199.2 207.9 205

172

195.4 199.9

0

50

100

150

200

250

GENSS/W Ins VT3/W Ins RR2/W Ins RR2 VT3 GENSS

Yield Rep 1 Yield Rep 2

Conclusions…

•History has shown the CRW species can overcome insecticides or traits

when conditions are favorable

•The Western CRW populations in these fields are extremely high

•More work needs to be performed in these “problem fields” to identify the

true issues

•There appears to be some type of tolerance building in these populations

of Western CRW to the Cry3Bb1 gene and intital data could suggest

tolerance to other single trait MOA providers

•The YEAR effect dictates performance of traits year-to-year

•Putting multiple MOAs together is showing great promise even with a

“so-called” ineffective trait like Cry3Bb1

•The sky is not falling

RECOMMENDATIONS GOING

FORWARD…

2012 Corn Rootworm (CRW) Recommendations

for Growers in High CRW Pressure Areas

• Rotate to a non-host crop • plant soybeans or another crop in order to break the insect population cycle in these local

areas…OR

• Plant Genuity® SmartStax® RIB Complete™

• a two mode-of-action product for CRW; it simplifies refuge management through the RIB

solution; it provides better insect control and ultimately, longer durability of our

technology…OR

• Plant YieldGard VT Triple® or Genuity® VT Triple PRO™ plus

a secondary mode of action

• Soil applied insecticide to control CRW larvae

• Aztec, Force, Counter

• Foliar insecticide application to control CRW larvae

• Very difficult to achieve success—moisture or tillage dependent

• Foliar insecticide application to manage CRW adults—very effective

2012 Corn Rootworm (CRW) Recommendations

for Growers in High CRW Pressure Areas

•Growers with positive experiences with single mode hybrids (VT3 VT3P)

and want to continue using these products

• Soil- & foliar-applied insecticides must be a part of this agronomic system when

high CRW populations exist.

•During the growing season—IPM

• High CRW pressure fields must be scouted for CRW larva feeding

• Understand the %Control with products being used and rescue if necessary

• High CRW pressure fields must be scouted for CRW adults late season

• Understand the emerging adult population,

silk clipping, leaf scarring and rescue if necessary,

and repeat if necessary

High CRW Pressure & Insect Resistance

Management (IRM)

•The importance of refuge compliance—IRM: • The primary purpose of a refuge is to maintain a population of insect

pests that are not exposed to the Bt protein. The lack of exposure to Bt

proteins allows susceptible insects nearby to mate with any rare

resistant insects that may emerge. Susceptibility to Bt technology would

then be passed on to their offspring, helping to preserve the long-term

effectiveness of Bt technologies

•With multiple modes of action in SmartStax® RIB

Complete™ your refuge is always in place

• RIB Complete™ = 95% SmartStax® + 5% Refuge

CRW Management Solutions for the Long Term

• 2012

• Switch crop rotation

• Plant Genuity® SmartStax® RIB Complete™ corn

• Plant YieldGard VT Triple® or Genuity® VT Triple

PRO® + Insecticides

• Future—Monsanto

• Cry3Bb1/RNAi CRW III*

• 2 RNAi +2 Bt genes in a vector stack CRW IV*

• Balance near-term performance and long-term durability

• Continued emphasis on IPM and the of planting the appropriate refuge

* Commercialization is dependent on many factors, including the successful conclusion of the regulatory process.

Summary…

•Mother Nature is still in control

– We sway her actions by the small things we do in our

farming practices and environment does the rest

•Profitability, ethanol / livestock production that demands corn

•These demands drive corn-corn rotations to satisfy the need

•More pressure gets applied to any one practice—traits, insecticide

•We have to be good stewards of the technologies

and agronomic practices at our disposal

Summary (cont.)…

•Don’t let the CRW populations get ahead of you

•Use multiple modes of action for your high pressure

fields

•Maintaining durability of these traits and practices is

the right thing to do

•When using traits, insecticides, rotations, report

problems or concerns to seed company to be

proactive

•The sky is not falling

Thank You