genotype and growth stage effects on heat stress

Genotype and Growth Stage Effects on Heat

Stress Susceptibility in Wheat

Henry M Barber Monogram 2016

Twitter: @henrybarber

[email protected]

Henry Barber – Monogram 2016

Heat Stress

Grain set failure in field grown UK wheat (but modified environment) associated with days above 30oC, during the five days before anthesis

Ferris R et al. 1998. Effect of high temperature stress at anthesis on grain yield and biomass of field-grown crops of wheat. Annals of Botany 82, 631-639.

Temperature at flowering (oC)

15 20 25 30 35 40

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ield

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0.2

0.4

0.6

0.8

1.0

Savannah

6 June

2010

Alghabari et al (2014) Effect of

Rht alleles on the tolerance of

wheat grain set to high

temperature and drought

stress during booting and

anthesis. Journal of Agronomy

and Crop Science 200, 36-45.

Effect of 3- day pot transfers


Susceptible Timings • Experiment in Rice:

SATAKE, T. & YOSHIDA, H. 1978. High

Temperature-Induced Sterility in Indica Rices

at Flowering. Japan Journal of Crop Science,

47, 6-17.

CRAUFURD, P. Q., VADEZ, V., JAGADISH, S.

V. K., PRASAD, P. V. V. & ZAMAN-ALLAH, M.

2013. Crop Science experiments designed to

inform crop modeling. Agricultural and Forest

Meteorology, 170, 8-18.


DH Population

• Savannah (Rht-D1b + 1BL/1RS) x Renesansa (Ppd-D1a x Rht8)

• 62 lines genotyped at JIC


Savannah (Rht-D1b + 1BL/1RS) x Renesansa (Ppd-D1a x Rht8)

BARBER, H. M., GOODING, M. J. & SEMENOV, M. A. 2014.

Improving modelling of wheat responses to high temperature

stress under climate change. European Society of Agronomy

13th Congress. Debrecen, Hungary: European Society of

Agronomy.


Project Overview

• Year 1:

• Heat stress trial on DH parents focussing on timing of stress

• Smaller Experiment on Paragon NILs

• Year 2:

• Heat Stress Trial on all DH population

• Most susceptible GS’s

• Assess for tolerance to HS


Identifying the susceptible timings

Stress Treatment

Control Treatment Spikelet weight

data,

Grain yield data

to be published. Published in:

BARBER, H. M., CARNEY, J.,

ALGHABARI, F. & GOODING, M.

J. 2015. Decimal growth stages for

precision wheat production in

changing environments? Annals of

Applied Biology, 166, 355-371.


Comparing the two Parents

• Significant effects on grain number

• Some grain size compensation at booting, especially

from Savannah

• Parents most susceptible at different stages

• Clear differences in response at anthesis

• Double Gaussian data relative to GS65 to be published.


Was it Rht8 Effects? • 5 timings in experiment

• Significant effects on grain number at booting and early

flowering

• Temperature x timing effects

• No significant effect of Rht8 on response

• Paragon NILs comparison comparing rht8, described in:

KOWALSKI, A. M., et al. 2016. Agronomic

assessment of the wheat semi-dwarfing gene Rht8

in contrasting nitrogen treatments and water

regimes. Field Crops Research.


Comparing the whole population • 3 timings in experiment: Early booting, Mid Booting, onset

of anthesis

• Significant variation in fertility response to high

temperature between genotypes

• Interactions with GS within each timing

• Allele effects at anthesis of Ppd-D1 and Rht-D1

• QTL analysis to be conducted

• Results published in near future


Key Points

• Double dip response seen

• Very discrete differences in HS response due to GS

• This response influenced by genotype

• Both timings need stress when comparing different genotypes

• Grain Size compensation variable due to genotype and

environment.

• Grain Number rather than yield needs to be studied

• No evidence Rht8 increases tolerance to heat stress

• Ppd-D1 and Rht-D1 influence fertility response at anthesis


Future Work

• Why are these effects around anthesis but not at meiosis?

• Why does Ppd-D1 and Rht-D1 cause differences in

response?

• Large variation in response in DH population

• Find out why unexplained variation occurred- QTL?

• Crop Modelling?


Thanks for Listening! • Acknowledgements:

• Mike Gooding, Martin Lukac, Mikhail Semenov

• BBSRC and the University of Reading

• Caroline Hadley, Liam Doherty, Richard Casebow, Laurence Hansen

• Richard Kino, Christina Clarke, Sam Leigh, Jacob Bishop, Pete Mark,

Peter Jackson, Mike Garratt

genotype and growth stage effects on heat stress

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