genomics: fish dna barcoding & protein profiling workshop
TRANSCRIPT
In electrophoresis, molecules such as DNA and proteins on top of an electric field migrate down toward
the opposite charged electrode and separate according to their charge and shape. This technique
revealsthe number of proteins found in each sample, their size and the similarities or differences
between them. Electrophoresis can be done byDNA barcoding, based on using a DNA strand to facilitate
species identification,in which various DNA are isolated into a lysis solution. Then, the primer mix is
prepared. Later, after setting up the PCR reactions, DNA are amplified in the thermal cycler, which are
then loaded into the agarose gel solutions. Electrophoresis can also be done by protein fingerprinting,
whichidentifies proteins and evolutionary relationships among fish, in which proteinsare placed in
microtubes containing a buffer. The samples are flicked, incubated andheated to denature the proteins
before beingloaded into the polyacrylamide gel solutions. After these techniques were presented in the
lab, an expert in invertebrate studies displayed his research on analyzing the taxonomy and genetic
patterns of Nemertia for being able to build a genealogical tree.