genesis 1:26
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Genesis 1:26 26 And God said, Let us make man in our image, after our likeness: and let them have dominion over the fish of the sea, and over the fowl of the air, and over the cattle, and over all the earth, and over every creeping thing that creepeth upon the earth. DNA Technology. - PowerPoint PPT PresentationTRANSCRIPT
©1998 Timothy G. Standish
Genesis 1:2626 And God said, Let us make man
in our image, after our likeness: and let them have dominion over the fish of the sea, and over the fowl of the air, and over the cattle, and over all the earth, and over every creeping thing that creepeth upon the earth.
©1998 Timothy G. Standish
DNA TechnologyDNA Technology
Timothy G. Standish, Ph. D.
©1998 Timothy G. Standish
What is DNA Technology?What is DNA Technology?Technology = The application of science in
industry and/or commerce.Because DNA is the genetic material,
manipulation of DNA (genetic engineering) allows the modification of organisms in such a way that they are more valuable than they were in their natural state.
©1998 Timothy G. Standish
Products of DNA TechnologyProducts of DNA Technology Recombinant organisms - Some examples:
– Herbicide resistant soy beans– Super tomatoes
Natural products produced by recombinant organisms:– Humulin - Human insulin produced in cell culture– Tissue Plasminogen Activator (TPA)
Engineered products produced by recombinant organisms:– Amplitaque - A modified DNA polymerase
Information:– Genetic testing for identification of diseases, individuals etc...– DNA sequencing
©1998 Timothy G. Standish
A Short HistoryA Short History 1869 - Miescher isolated DNA for the first time 1944 - Avery et al. provided evidence that DNA is the genetic
material 1953 - Watson and Crick described the double helix structure of
DNA 1957 - Kornberg discovered DNA polymerase 1961 - Marmer and Doty discovered DNA renaturation 1962 - Arber, Nathans and Smith discovered restriction
endonucleases 1966 - Nirenberg, Ochoa, and Khorana figured out the genetic code.
©1998 Timothy G. Standish
A Short History Cont.A Short History Cont. 1967 - Geller discovered DNA ligase 1972-73 - Boyer, Cohen and Berg develop DNA
cloning techniques 1975 - Southern developed gel-transfer
hybridization 1975-77 - Sanger and Barrel and Maxam and
Gilbert developed DNA sequencing methods 1981-82 - Palmiter and Brinster produced transgenic
mice, Spradling and Rubin produced transgenic fruit flies
1985 - Mullis and colleagues invented the Polymerase Chain Reaction (PCR)
©1998 Timothy G. Standish
StrategyStrategyExtract DNA
Fragment DNA
Insert into vector
DNA Library in bacteria
Screen library and grow up bacteria with clone of interest
©1998 Timothy G. Standish