bioMérieux S.A.

69280 Marcy l’Etoile, France

Tél. : 33 (0)4 78 87 20 00

Fax : 33 (0)4 78 87 20 90

www.biomerieux.comwww.biomerieux-industry.com

1 Detection

Reconstitution

buffer

E. coli O157:H7

PCR Mix

Lysis

5 min

5 µL5 µL

Spin down 10 sec

<1 h run

- +

45 µL

2 Confirmation

ChromID®

O157:H7

24 ± 3 h at 35 ± 1°C CT-SMAC

24 ± 3 h at 35 ± 1°C

CONFIRMATION

Enriched sample

200 g in 800 ml

pre-warmed

BPW

10-24 h at 42°C ± 1°C20 µL

E. coli O157:H7 rangeREF 414152 GENE-UP® E. coli O157:H7 Kit 192 testsREF 414057 GENE-UP® Lysis Kit 192 testsREF AEB910303/4 BPW broth 4 x 3 LREF AEB910305/2 BPW broth 2 x 5 LREF 42606 Cefixime-Tellurite Mixture (CT)REF 43391 CT-SMAC agar 20 platesREF 42605 chromID® O157:H7 agar 6 x 200 ml REF 42630 chromID® O157:H7 agar 5 x 20 ml

GENE-UP® E. coli O157:H7 Produce

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GENE-UP® E. coli O157:H7 Raw Beef

1 Detection

E. coli O157:H7 rangeREF 414152 GENE-UP® E. coli O157:H7 Kit 192 testsREF 414057 GENE-UP® Lysis Kit 192 testsREF 42043 BPW broth 6 x 225 mlREF 42729 BPW broth 10 x 225 ml mini-bagsREF 42042 BPW broth 6 x 90 mlREF AEB910303/4 BPW broth 4 x 3 LREF AEB910305/2 BPW broth 2 x 5 LREF 42606 Cefixime-Tellurite Mixture (CT)REF 43391 CT-SMAC agar 20 platesREF 42605 chromID® O157:H7 agar 6 x 200 ml REF 42630 chromID® O157:H7 agar 5 x 20 ml

Reconstitution

buffer

E. coli O157:H7

PCR Mix

Lysis

5 min

5 µL5 µL

Spin down 10 sec

<1 h run

- +

45 µL

10-24 h at 42°C ± 1°C

8-24 h at 42°C ± 1°C

375 g in 1.125 L

pre-warmed

BPW

25 g in 225 ml

pre-warmed

BPW

2 Confirmation

ChromID®

O157:H7

24 ± 3 h at 35 ± 1°C CT-SMAC

24 ± 3 h at 35 ± 1°C

CONFIRMATION

20 µLEnriched sample

bioMérieux S.A.

69280 Marcy l’Etoile, France

Tél. : 33 (0)4 78 87 20 00

Fax : 33 (0)4 78 87 20 90

www.biomerieux.comwww.biomerieux-industry.com

bioMérieux Industry

595 Anglum Road

Hazelwood, MO 63042 • U.S.A.

Tel: (800) 634 7656

Fax: (314) 731 8678

bioMérieux Canada, Inc.

7815 Henri-Bourassa Boulevard West

St. Laurent (Québec) • Canada H4S 1P7

Tel: (1) 514 336 7321

Fax: (1) 514 807 0015

www.biomerieux-usa.com