DNA SEQUeNCing SummaryDeoxyribonucleic Acid Watson and Crick modelDNA Sequencing PurposeDNA Sequencing MethodMaxam & Gilbert MethodSanger MethodComparison Application of DNA Sequencing Advantages & Disadvantages

Shotgun sequencing

Direct approach

Direct approach

Next generation DNA sequencing

Next generation sequencing platforms differ from traditional Sanger sequencing technology in a number of ways.

First, the DNA sequencing libraries are clonally amplified in vitro, obviating the need for time consuming and laborious cloning of the DNA library into bacteriaSecond, the DNA is sequenced by synthesis, such that the DNA sequence is determined by the addition of nucleotides to the complementary strand rather through chain termination chemistry.

Finally, the spatially segregated, amplified DNA templates are sequenced simultaneously in a massively parallel fashion without the requirement for a physical separation step.