Gene Query

http://www.ncbi.nlm.nih.gov/

HBB gene for homo sapiens

Our query will give the following results

Sequences available

Proteins available

Expression data, available from several high-throughput transcriptome technologies.

Micorarray Experiment

Sequences available

We check forsequences

Info concerning the GENE

Info concerning theHBB Nucleotide Sequence

Lets check the nucleotide sequence by opening the link

Info concerning the GENE

Info concerning theHBB Nucleotide Sequence

Info for the sequenceAccession from RefSeq DB

Length Latest annotation

“vesrion” and “gi” accessions:

CHECK LINK FOR INFO:http://www.ncbi.nlm.nih.gov/Sitemap/sequenceIDs.html

Lower part of the previous file

Additional links

More choices of visualizingthe sequence and the related information

More choices of visualizingthe sequence and the related information

Lets choose the coding region (CDS).This will give the minimum information concerning Geneand CDS (see next slide)

Start end of GENE

Start end of coding region

Link to other databases

Lets extract the coding sequenceby clicking at the CDS link

It opens the part concerning the cds

Check the different (lower) length to that of the gene

Upper part of the file

Lower part of the file terminateswith the coding sequence

HOW TO EXTRACT

Above we have the option to choose the format.Since we want to extract the Coding sequence, we choose FASTA format.

WE SAVE THE NUCLEOTIDE SEQUENCE OF HBB.WE MAY NOW OPEN AND VISUALIZETHE SAVED SEQUENCE USING A TEXT EDITOR

LETS GO BACK TO THE INITIAL QUERY

Lets check the GENE INFORMATION

Info concerning the GENE

Info concerning theHBB Nucleotide Sequence

SEVERAL FEATURES FOR THE GENE UNDER EXAM

GENE STRUCTUREPlus Genomic info

Info for the 5’ and 3’ UTR,introns and exons.

Information for position on GENOME

GENOME VIEWER

OUR GENE