gene query
DESCRIPTION
Gene Query. http://www.ncbi.nlm.nih.gov/. HBB gene for homo sapiens. Our query will give the following results. Sequences available. Proteins available. Expression data, available from several high-throughput transcriptome technologies. Micorarray Experiment. Sequences available. - PowerPoint PPT PresentationTRANSCRIPT
Gene Query
http://www.ncbi.nlm.nih.gov/
HBB gene for homo sapiens
Our query will give the following results
Sequences available
Proteins available
Expression data, available from several high-throughput transcriptome technologies.
Micorarray Experiment
Sequences available
We check forsequences
Info concerning the GENE
Info concerning theHBB Nucleotide Sequence
Lets check the nucleotide sequence by opening the link
Info concerning the GENE
Info concerning theHBB Nucleotide Sequence
Info for the sequenceAccession from RefSeq DB
Length Latest annotation
“vesrion” and “gi” accessions:
CHECK LINK FOR INFO:http://www.ncbi.nlm.nih.gov/Sitemap/sequenceIDs.html
Lower part of the previous file
Additional links
More choices of visualizingthe sequence and the related information
More choices of visualizingthe sequence and the related information
Lets choose the coding region (CDS).This will give the minimum information concerning Geneand CDS (see next slide)
Start end of GENE
Start end of coding region
Link to other databases
Lets extract the coding sequenceby clicking at the CDS link
It opens the part concerning the cds
Check the different (lower) length to that of the gene
Upper part of the file
Lower part of the file terminateswith the coding sequence
HOW TO EXTRACT
Above we have the option to choose the format.Since we want to extract the Coding sequence, we choose FASTA format.
WE SAVE THE NUCLEOTIDE SEQUENCE OF HBB.WE MAY NOW OPEN AND VISUALIZETHE SAVED SEQUENCE USING A TEXT EDITOR
LETS GO BACK TO THE INITIAL QUERY
Lets check the GENE INFORMATION
Info concerning the GENE
Info concerning theHBB Nucleotide Sequence
SEVERAL FEATURES FOR THE GENE UNDER EXAM
GENE STRUCTUREPlus Genomic info
Info for the 5’ and 3’ UTR,introns and exons.
Information for position on GENOME
GENOME VIEWER
OUR GENE