gate life sciences 2000

8/13/2019 GATE Life Sciences 2000

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IG,\ E-ZO{) l I ot 16LIFE SCIENCES Jc:===========

H 1 HEMISTRY COMPULSORY)Usefullnfonnalion:

Gasconstanl R = Jmol ~P l a n e s conswu . h li.626 l0 J sMass ol'eleclron, mo =9 10&-x 10 " kg

Foo cacb sub-qucsHon oo siatenwnt givenbelow, fo ur ah emati ves a oe giveu or whk h onlyon e is cor er t. \rite th cor red answer in fbeanswer book by woiting a, b, c or d along wi thche cb rrespondin_: sub quescion o stnre me ntn u m b (15 x 1 15)

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Wh ch one of the followmg elemen ts hasthe largest mdius'/a. Beb cc Nd F'The ''nlenceeleclronic c o n ~ g r a l i o of' Cratom lAiomic Numb er 24) in i ls groundstate isa. -ls13d5b. 3d ' 4s1c. -ls23d'd. 3d ' 4sl'he phosphorus 0101\1m lhe mism the Sla te ofhybridi1.a110na splb spc. p'd. dspThe 1onic compounds MgO and CaO hal ethe same cryslal structure excep1 lhal theintenonic distanees are 2 Ill A and 2 40"A res pecthely The ratio of latucel."llergies, U(MgO)/U(CaO). isa. 0.7b. ) &75c I 14.3

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d. 1.30i ng relationships holds good i f hereis no c.hange .n the number of mo les due 10the a c b oa K;, ~ Kb Kp > K.c. < -;.d K, =Vz KLo t PI and p, be the ,.ap


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a. Beb. cc. Nd 0l'hll oxidations tate ofBt in B:10, 1sa. zerob. Ic. +2d 14One of tlte species given bel ow 1S a Lewisacid. Which one is it?u. Ib. rc. I ,d l13. lde nlifY 1e mOSl stable :;pecies from lbefollowing divalent halides;a. CCI,h. ~c. n ~d PbClt

14. Wluch of the fallowing ligands causes lbemaxirnwn crys1al field f lil1ing wh ileforming a complexwith Pe2 1on'(a. Ob, NH,c. c ~d. cr15 The formal oxidation stare of Ni inNi(CO), isa. zerob. +Ic; -t2d +4

For cacb sulrqucslion or : ~ t e u w o l ginobelow, fow- alternatives a.le gheo ofwblcb onlyoue Is conoct. W r ite tht> con-oct n ~ w e r In the~ w e r hook by writing a , b, c or d along \\itbthe corresponding u b q u e ~ 1 i o or >t-atementnumber. ( h1II , The largest ionization energy is tbund with

5 . atkali metalsb. alkriline u 1 J J metalsc, halogens

2 of l6d. inert gasesI 7_ In Planck's l1ypothesis E = bv, E is theenergy of

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a. aphotonb. a hydrogenatomc. one clectrond. one H. o l e cThe most IVid


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0'rhe ICachou ol 1-uctyne with JlgSU,Jl ,SO, Ll10 will l' ''ea. nclnn- luncb. qornn 2 onuc. clnn-J..outd. t - 1 - e u ~Tho correct order of dccrcnsing mtc )dehvdrohnlnuonnuon n(ulkvl hnlidcs ts- - -a. RBP Rl RCI > RfuRl RBr RCI Rf

Rl ROr- Rl' RCId. Rl RF RBr RC I

Z5 TI1c reaction o( sodiumpbcooxide withCQ at high lCmpcrulure und pres ;l trctallowcd byaddilicntion givesM. p - h y d r o x ~ b.:uzoic acidb. m-hydroxy bcnzo1c uc,1dc, oh)'droxy bcw.flic acidd. p-hydm:.-y sodium benzoate

26. i\ns\\erthe Iolli ing .I C u l c t h ~ l1>11ice


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c. PhophoryJ.-c:tl. Glyt.:rlgtm syntl lt:t.a :seThe tumoyer number of chymoh flsin l1100 s 1 and fo r DNA polymernse ll ,.s t. "lltis means th:Jt

ChymolrYP"in hind' I() it s u b s t r a t"illt higher a J l i ~ i t y tlum uocs DNApnlymer:,.cb. The vdoC'rty of tl1c chymotrypsinreacti(m Ill a h > O greater limn lhal ,,f'DNA polymerasen,., ve lollitieh. lO"oc 7 1 ~ ' .d. 151\o

10. The follow ina typeofmteraction is moin lyresponsible lo ; ~ t i o n ..,r proteins indilute so lutionsa. llydmgcn bundsb. H intc:ntcti


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c. lUO.UUU [oldd, 10 folrlA homogeneous protein of oativemolecular 1wrght I 00,000 ~ v e singlebandof nolecular weight 50.000 on SDS -PAGE u presence ofj)-mercaptoethanol.N-tenniml analy.;i gave two amino acidlllarune and leuollie in equal proportionsHortec tho

.Protein is a lx>modimerb. Protem J s contaminated w1lh. U 1 ~ r'rol emo. Protoit1 ha.< l.wo ptilype mcolum n 2 -.nd wnte mn lchms pairs in lhenswer book Sx 1=5)

Co1wnn 1A. Van tle1 Waals n d ~Ll 'lU lobulu:rs

Teniruy siructurcD. H i l l c o e f f i c i e n l ~ n HE Tu01camycinCoh,nnn

St:>atial ammgemeol of amino acidsll.Jat are neru each odJel m the I nearsequcuco

2 Glycosy lataon 1 t o r3. Coope.mliv ity of oxyg.au bimliog4 r n v o l v e ~ polarizable5 lrurmnw protection

22 Answer lhe l l o w ~A. In U1c-Entne.r - Doudoroff pathway. 2-keto-3-


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B. T o tirst order rote constlll tsk, tutd kJand one .second order rote constant 1.:1delint k., by tht relationship.K ~

II 1llv subslltuting the appropriate. muts fott J ~ rate. Cotl..SlftD IS IU this C..Xp i\ 'SSltlU. sbowLltut K., IIIUXI be x p r t S . ~ e d Il l Lei111S orconcetJt rutum . {2)Auswet tilUu11iug .A. rseuak. sUl .. 1s an uncuupllngreagent lor O. C1dauvc phosphorylahoo

b 111 unhke dinllrnpheuul 11 does 1101L r a u s p ~ . u t protons uctoss the innermitochoulriu l l b r u n e How 111ightursenate [ul\Ctiuple r? (31

13 . Romuval uf the thymus glund from uyoung unu11uJ uot only CUiupruutiscsUte prducriou or tn ii)X.ic Icdls. butnlso decreases antibody p1'oduction b)B Why is this the u . s ~ (2)

Ausii' r ~ o t irnte,he rcspirulol)' quoticn t wuu ld 1 >u. 0.36h 11.80c. I (XId 1.3 1

W h ~ t role does 11ccrosyring


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tL Indu= site -pecific nicks in lhebo lom strandl'he pro ce. Ah.cilr ic .reidc Zeot ind. GibborcllinsCnrn (Zcu mny ) a rli1>l1id number of2(). 'llte chromosome number in rnicm p


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An o m i n t h e t 3 ~ u responslbleform lion of peptide bond

1>. f:lind ing ofmRNA lo ribosomes, grn.:.ifu.c.. Ambidop. ill ~ o i l l iJ . RJ IJ)O di>co lornu: pb ys iolo_gi1:B. MentholC. ~ n mD. VincnlmeE. Opium (5)Anwcr the rn llowongA, EJH.unernte three imrortru11 g ~of liquioculture in micr-propagnlion

ofplants. 1 ~ 1B. J, i> l two fa"tors tha i nJlillo.M molec ular m:orkc:rMhave.ocred v e r ~ a wol in plnn1 gcnn(lfy:


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Wl>ich one 111 lhe folluwing ~ t n H < n > t 1correcl'/n, Grnm +ve bocteno lack p;:p1idoglycnnh (ir:>m hnc1crio lock pC(llidoglycon,e. Bolh (;ram and n nnn -ve h;ICterl:l

Jli SI'SS p c : p t i d y ~ ; a and Uu: outermcmbn11d. BoO> Gram ' 'e nnd G1':1 111 -ye bucteri11possess pcplidoglycou bul 01c Gram+vc b3cteria lacok- outer memhNm :

For infeclion of Esd,ericlun c:uil 1\ irh lhel;unbda l e , the E. ct>li is u.onlly ST''in a medium \ . " o n t ~ t i . n l n g m:Jitosc b e c . : a u . ~ en. l'.bllose is essenli>l for lhe lys i" of E.euli.b Maltose >orvcs os an imporL'llllcofaclor during t h ~ phnge DNA

rephe>hon,.:. The presence of moltosc mduce.moillls" binding prutvin whi


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c. Presence of viral p a r t i ~ l e 3 in the lio.llowing M t c m onphnhlrespir>tiun is ineorrect7a. II occurs on ly ln phot lc. DN1 polymcrnocd. Rc:yersa trnnscriptas.a

T i t . ~ drug. i c i n e ~ x ~ r t s l inhibitoryaclion during which or the tb llowinsa s ~ of rhe c.:ll cycle'/

a. G )h. Ci Ic. (.i2d. MWhich nf tb" followmg lnlamf lltJ iwnmg/ . Lactic aood is produced bv

L o c t o b o d l l u ~ dolbrueckiih. Bout.,rial amyln."; l>roduced hy

B ~ < : i U W I subl illsc. Cilt '" acid b produccd by Rlrizopusd. Gibherollic ncid is produced byFusa1ium mono liforme .l) unng I)Nt\ replicotion, the ynthc.is Ithe lcuJing strnnd uc< un; in the 5dircctioo n wilh lhc muvc:mcul t,r he::IUJ;IicMiun fork \ \ ~ r i c h one of lhcfil lowing " rmc uho t rhc

s y n t l o o ~ v ; or tloc l>ggmg strand?" DNA ~ r n s e U. which hs anaddiliouol udivil)' of pol ymo r-iL.ttion in3' s dilwtJon, is utilizOO.b. DNA pnlynoern" T is ulilizod whichcnn carry our ONA svnthest inabsence ;Jr an y prin1cr c. ntlt


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.ympatrio populotions shown. P


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ca roohydril tC i y ~ O l l l n . hen the ell j ;tramn. Plantb. Pungo B ~ c t e r i ~.1. AnimalIn hum ans. the implantation or blastocystoccurs tn. lmmotliatc:ly oller w ulationb. 1-2 rlnys after om latCinc 6-8 days after-oV11 IaHond. 2-5 day> ~ n c r mtlalionl11e pollem nl" gfO\\ th in primnto" IJmitcd and ollomelrieb Limited rmd isometric. . u nlimited and i.mnehi.:.d. L nlimitcd aod aUomctricllumoulic mui.1Lit.lus rosult ina, L-o of H o m c o s l > l l i . ~b. L o . ~ of l10111ing ol' prowin. to tlteir

< ~ r g c l .:_ Delation of body partd. Conversion ofone o d y pnrl o anotltor13n.:ili"'Y dyseot"TY i lll Oduced h)' lh.:argnnism bclQnging to the genesIL. Baoillusb. Shigdla.:, Myeob>cletiumd. Cl01> tridiumr>.titolic p l n i l l is l'ocued by bundle5 orn. t u h u lh. l l l i c r o t i l u n e u t ~c J.llcu>bodi"su. Jntermediate ill:unt u15lnltrpnolhtinn of bchaviornl datA in l c m \of humuu C.XIIl.Ticm:c ;, calleda. . \.nUtrOpocculrismb. n lhropruno pllliruc Tc Vide brief and prccu;c nnswcr I


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26 Provide brief o.nd precise onswor to each ofhe que: txoco producedcomm ercia y from m o m m a l i ~ n cellcultures?a, sm.inogeo actNatDrb, Antibactrial antibiotic$c. Imulind. Re nin1 .ung b.:nn nucltmsu could bc usud foa, DNAsyn tb es.i.s

b. uuchcotidc hydrolysisc. trlaruulng single slnlndad r ~ : g a o n inDN \d. rcmo l of f'l10sphllt< from the

> n e l nflhe DNAPhage T7 pi'Omotcr contnining mare u.sed frw uvcrcxprcssivn c)l clnnt:d

' l l . < b c'Cllll


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cL T7 infeclll E. coli lysogenizcs the.:ellIn e u k a r y ~ the nb()S>t un: bi 'hly lil.o

n.ntibCII Iiosb. Antibodiu tltllt hn \'Cc;llulytic c t i \ i i c . ~.:. .' \so ret'erred l.)'tttoglllUd. &zymcs wllicidtydroi)>N\lihrary in vector tha t allows the.:xpresiou s a fusion with a reporter tllg.\Vllllt p ~ r c c n l l t g uf the eDNA do ucs . like ly to give rise to oom:ct gene produ ~ d ~ tor prel llanc)' m

humaM?


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,._ Ulb. Fl'iHc. Chruionic gonadotropin,L Btroscn

25. PKL ;,. uno known hcn:l :utlibodk-s n::oxtensivaly in di:agno1ish emlltUpoiertc C:Jocer tAMwcr the foll(lwmg

ucdof

(5)A. Explin in one sentence why you can

nHl tun n < ~ l anllbddfes wln\ .h.:;ou react wiUt uo:ISt cells. (2)

B. W l ~ l .,c b b 1 ) e c i i i u1onoolonal..nliboJi t:s1 (1)C. lJO I bispccific I I I O U O C I M ~ antibodie&can be genenucd .

Give two methods ) (2)t.bc following

A. Wlul iHomulio m b r y o g e n ~ i . s ( IIB. \Vll i lhc difference ~ 1 W I . l C I t l i r e ~ lnil indirect orn tic emhryogenei'l

{2)C. IIVO methods for direct l)N1

a n ~ f . : r mto plant c.:lls (2)Am;w

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