gametogenesis in the adult hermaphrodite of c. elegans
TRANSCRIPT
oocyte eggs inuterusspermatheca
gonad
vulva
Gametogenesis in the adult hermaphrodite of C. elegans
Chromosome structures in the gonad of the adult hermaphrodite
0-8h: II (early embryogenesis) + III (diakinesis oocyte)8-24h: III + IV + V + VI (diplotene to pachytene nuclei)
Egg hatchability in the wild-type hermaphrodite after X-ray irradiation
(0- 8 h) (8 – 22< h)
Egg hatchability in the wild-type hermaphrodite after heavy ions and UV irradiation
Requirement of Ce-rdh-1 & Ce-atl-1 genes for ds-break repair system
Requirement of Ce-atl-1 gene for UV-damage repair system
CED3 apoptosis system is dispensable for ds-break repair system in meiotic cells.
Conclusion
(1) Meiotic pachytene nuclei were hyper-resistant to X-ray, 12C, and 18Ar-ion beam irradiation.
(2) It suggests that general recombination activity during meiosis can effectively repair ds-breaks and gaps by X-ray, 12C, and 18Ar-ion beam irradiation.
(3) A checkpoint kinase Ce-atl-1 (ATM/ATR like 1) was also involved in repair for X-ray and UV damages of mitotic and meiotic cells.