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TRANSCRIPT
B. K. Birla College (Autonomous), Kalyan
Department of Biotechnology
F.Y.B.Sc.
Syllabus for Autonomy
Year
2018-19
Preamble Twenty First Century is known as the ‘Century of Biotechnology’. Biotechnology is one
of the youngest branches of Life Science, which has expanded and recognized as an
advanced interdisciplinary applied science in last few years. Biotechnology at the core
envisages the comprehensive study of Life. The Interdisciplinary aspect of Biotechnology
has led to a unique and dynamic status for this subject in the field of Research and
Industry.
The socio-economic potential of Biotechnology is well established. Biotechnology has its
applications in almost every field touching practically every human activity. The applied
aspect of Biotechnology is now getting recognized with its applications in various
industries viz. Agriculture, Health, Environment.
Biotechnology demands trained and skilled human resources to establish the Industry and
Research sectors. The field is novel and still expanding which demands inputs in
Infrastructure and Technology. The global and local focus is on developing new
technological applications are fast growing. Biotechnology sector in Research and
Industry is expanding which is set to predict the next major revolution in the world.
The demand for trained workforce in Biotechnology is ever growing in Fundamental
Research and Industry Sector. Academic and Research Sectors also require
interdisciplinary trained manpower to further the Biotechnology Revolution.
The need of the hour is to design appropriate syllabi which keeps pace with changing
times and technology with emphasizes on applications while elucidating technology in
depth. The present Syllabi is restructured anticipating the future needs of Biotechnology
Sector with more emphasis on imparting hands-on skills. The main thrust is laid on
making syllabus compatible with developments in Education, Research and Industrial
sectors. The Theory and Practical course in new restructured course will lead to impart
skill-set essentials to further Biotechnology Sector.
The restructured syllabus combines basic principals of Physical, Chemical and Biological
sciences in light of advancements in technology. The curriculum aims to impart basic
knowledge with emphasis on its applications to make the students industry ready.
F.Y.B.Sc.
SEMESTER I Credits
BUSBT101 Basic Chemistry -I 02
BUSBT102 Biochemistry- I 02
BUSBT103 Basic Life Science-I 02
BUSBT104 Basic Life Science-II 02
BUSBT105 Biotechnology-I 02
BUSBT106 Biotechnology-II 02 BUSBT 107 Ability Enhancement Course 1 02
Practical
BUSBTP101 Basic Chemistry 02
BUSBTP102 Life Sciences 02
BUSBTP103 Biotechnology 02
SEMESTER II
BUSBT201 Basic Chemistry- II 02
BUSBT202 Biochemistry- II 02
BUSBT203 Basic Life Science-I 02
BUSBT204 Basic Life Science-II 02
BUSBT205 Biotechnology-I 02
BUSBT206 Biotechnology-II 02
BUSBT207 Ability Enhancement Course 2 02
Practical
BUSBTP201 Basic Chemistry 02
BUSBTP202 Life Sciences 02
BUSBTP203 Biotechnology 02
SEMESTER I SEMESTER II
Basic Chemistry -I Basic Chemistry- II
Unit I Nomenclature and classification
of chemical compounds
Thermodynamics
Unit II Chemical Bonds Chemical Kinetics
Unit IIII Water and Buffers Titrimetry and Gravimetry
Biochemistry- I Biochemistry- II
Unit I Carbohydrates Amino acids and proteins
Unit II Lipids Enzymes and Coenzymes
Unit IIII Nucleic acids and genetic
material
Analytical techniques
Basic Life Science-I Basic Life Science-I
Unit I Animal and Plant Diversity Plant Physiology
Unit II Introduction to Microbial
diversity
Animal Physiology
Unit III Classification of Bacteria Classification and cultivation of Viruses
Basic Life Science-II Basic Life Science-II
Unit I Sterilization techniques Prokaryotic Cell Organization
and Division
Unit II Nutrition, cultivation and
enumeration of microorganisms
Eukaryotic Cell Organization and
Division
Unit III Microscopy and stains Ecology: Biogeochemical cycles
Biotechnology-I Biotechnology-I
Unit I Scope of Biotechnology Prokaryotic and Eukaryotic Replication
Unit II Applications in Biotechnology Microbial genetics
Unit III Ecology and interactions Population genetics
Biotechnology-II Biotechnology-II
Unit I Mendelian and Non-Mendelian
inheritance
Model organisms in biology
Unit II Eukaryotic genome and
Chromosomal disorders
Basic Immunology
Unit III Basic Biostatistics DNA mutations and DNA repair
Ability Enhancement Course 1 Ability Enhancement Course 2
SEMESTER I Credits
BUSBT101 Basic Chemistry -I 02
Unit I Nomenclature and classification of chemical
compounds
1.1 Nomenclature and Classification of Inorganic
Compounds: Oxides, Salts, Acids, Bases, Ionic,
Molecular and Coordination Compounds
1.2 Nomenclature and Classification of Organic
Compounds: Alkanes, Alkenes, Alkynes, Cyclic
Hydrocarbons, Aromatic Compounds, Alcohols
and Ethers, Aldehydes and Ketones, Carboxylic
Acids and its derivatives, Amines, Amides, Alkyl
Halides and Heterocylic Compounds
15
Lect
ures
Unit II Chemical Bonds
2.1 Ionic Bond: Nature of Ionic Bond, Structure of NaCl,
KCl and CsCl, factors influencing the formation
of Ionic Bond.
2.2 Covalent Bond: Nature of Covalent Bond,
Structure of CH4, NH3, H2O, Shapes of BeCl2, BF3
2.3 Coordinate Bond: Nature of Coordinate Bond
2.4 Non Covalent Bonds: van Der Waal’s forces:
dipole - dipole, dipole - induced dipole.
2.5 Hydrogen Bond: Theory of Hydrogen Bonding
and Types of Hydrogen Bonding (with examples of
H2O, RCOOH, ROH, Salicylaldehyde, Amides and
Polyamides).
15
Lect
ures
Unit III Water and Buffers
3.1 Properties of Water, Interaction of Water with
Solutes (Polar, Non-Polar, Charged), Non-Polar
Compounds in Water – Change in its Structure
and the H ydrophobic Effect, Role of Water in
Biomolecular Structure and Function and Water
as a Medium for Life
3.2 Solutions: Normality, Molarity, Molality, Mole
fraction, Mole concept, Solubility, Weight ratio,
Volume ratio, Weight to Volume ratio, ppb, ppm,
millimoles, milliequivalents (Numericals expected).
3.3 Primary and Secondary Standards: Preparation of
Standard Solutions, Principle of Volumetric
15
Lect
ures
Analysis.
3.4 Acids and Bases: Lowry-Bronsted and Lewis
Concepts. Strong and Weak Acids and Bases - Ionic
Product of Water - pH,pKa, pKb. Hydrolysis of Salts.
3.5 Buffer solutions –Concept of Buffers, Types of
Buffers, Derivation of Henderson equation for Acidic
and Basic buffers, Buffer action, Buffer capacity
(Numericals expected.) pH of Buffer Solution.
Ref:
University General Chemistry , 1st edition (2000), C.N.
R. Rao, Macmillan Publishers, India ,
Physical Chemistry University for biological sciences,
1st edition, (2005), Chang R., Science Books, USA
Essentials of Physical Chemistry, 24th edition, (2000),
B S Bahl, G D Tuli, Arun Bahl, S. Chand Limited,
India.
Concise Inorganic Chemistry .5th edition (2008),
Author: J. D. Lee, John Wiley & Sons, USA.
Organic Chemistry, 6th edition, (1992), Morrison
Robert Thornton, Pearson Publication, Dorling
Kindersley (India Pvt. Ltd.)
BUSBT102 Biochemistry- I 02
Unit I Carbohydrates
1.1 Classification, Structure and functions
Monosaccharides: D & L Glyceraldehydes,
stereoisomerism, classes of monosaccharaides,
structure of monosaccharaides, Disaccharides:
Structure, source and functions; Polysaccharides:
Homo- and Heteropolysachharides; Storage and
Structural polysaccharides.; Derivatives and
glycoconjugates
1.2 Characteristic reactions, Physical and chemical
properties
1.3 Carbohydrates as informational molecules
1.4 Carbohydrate analysis and glycomics
15
Lect
ures
Unit II Lipids
2.1 Classification, structure and properties of Lipids:
2.2 Fatty acids (saturated and unsaturated fatty acids,
nomenclature), waxes, triacylglycerols,
phospholipids (Lecithin, Cephalin, Plasmalogen),
2.3 Storage lipids and structural lipids
2.4 Lipoproteins: Structure and Function
2.5 Lipid aggregates: Micelles and bilayers, liposomes
2.6 Rancidity, saponification, lipid peroxidation and
15
Lect
ures
hydrogenation of Oils, Tests to check adulteration
of fats and oils,
2.7 Action of Phospholipases
2.8 Lipids as signals, cofactors and pigments
2.9 Lipid analysis: Extraction, separation and
identification of lipids
Unit III Nucleic acids and genetic material
3.1 DNA, RNA.
3.2 Structure of Purine and Pyrimidine Bases,
3.3 Hydrogen Bonding between Nitrogeneous Bases in
DNA,
3.4 Differences between DNA and RNA,
3.5 Structure of Nucleosides, Nucleotides and
Polynucleotides,
3.6 Different form of DNA and RNA.
15
Lect
ures
Ref:
Outlines of Biochemistry: 5th Edition, (2009), Erice
Conn & Paul Stumpf ; John Wiley and Sons, USA
Principles of Biochemistry, 4th edition (1997), Jeffory
Zubey, McGraw-Hill College, USA
Lehninger , Principles of Biochemistry. 5th Edition
(2008), David Nelson & Michael Cox, W.H. Freeman
and company, NY.
Fundamentals of Biochemistry. 3rd Edition (2008),
Donald Voet & Judith Voet , John Wiley and Sons, Inc.
USA
Biochemistry: 7th Edition, (2012), Jeremy Berg,
Lubert Stryer, W.H.Freeman and company, NY
BUSBT103 Basic Life Science-I 02
Unit I Animal and Plant Diversity
1.1 Origin of Life, Chemical and Biological Evolution,
1.2 Origin of Eukaryotic Cell.
1.3 Concept of Biodiversity, Taxonomical,
1.4 Ecological and Genetic Diversity & its
Significance
1.5 Introduction to Plant Diversity: Algae, Fungi,
Bryophyta, Pteridophyta, Gymnosperms and
Angiosperms (with one example each)
1.6 Introduction to Animal Diversity: Non-Chordates
and
Chordates {with at least one representative
example.)
15
Lect
ures
Unit II Introduction to Microbial diversity
2.1 Introduction to Microbial Diversity
15
Lect
2.2 Archaebacteria,
2.3 Eubacteria,
2.4 Blue-green Algae,
2.5 Actinomycetes,
2.6 Eumycota Habitats, Examples and Applications.
ures
Unit III Classification of Bacteria
3.1 Bacteria : Classification, Types, Morphology (Size,
Shape and Arrangement)
3.2 Cultivation of Bacteria.
3.3 Reproduction and Growth (Binary Fission,
Conjugation and Endospore formation)
3.4 Growth Kinetics, Isolation and Preservation.
Significance of Bacteria
15
Lect
ures
Ref:
Jordan, E.L. and Verma P.S. 1978, (i) Chordate
Zoology S. Chand & Company Ltd. Ram Nagar. New
Delhi.
Modern Text Book of Zoology: Invertebrates.
R.L.Kotpal. Publisher, Rastogi Publications, 2012.
Devlin R.M. (1983) - Fundamentals of Plant
Physiology (Mac. Millan, New York)
Dutta A.C. (2000) A Classbook of Botany (Oxford
University Press, UK)
Ganguli, Das Dutta (2011) – College Botany Vol I, II
and III (New Central Book Agency, Kolkata)
Ecology – P.S. Verma and Agarwaal- S. Chand
Publications
Microbiology–6th Edition (2006), Pelczar M.J., Chan
E.C.S., Krieg N.R., The McGraw Hill Companies Inc.
NY
Presscott‘s Microbiology, 8th edition (2010), Joanne
M Willey, Joanne Willey, Linda Sherwood, Linda M
Sherwood, Christopher J Woolverton, Chris
Woolverton, McGrawHil Science Enginering, USA
Text book of Medical Microbiology, Anantnarayan
Microbiology- Frobisher
General Principles of Microbiology- Stanier
Fundamental Principles of Bacteriology - A. J. Salle
McGraw Hill
BUSBT104 Basic Life Science-II 02
Unit I Sterilization techniques
1.1 Definition : Sterilization and Disinfection.
1.2 Types and Applications Dry Heat, Steam under
pressure, Gases, Radiation and Filteration
15
Lect
ures
1.3 Chemical Agents and their Mode of Action -
Aldehydes, Halogens, Quaternary Ammonium
Compounds, Phenol and Phenolic Compounds,
1.4 Heavy Metals, Alcohol, Dyes, and Detergents
Ideal Disinfectant.
1.5 Examples of Disinfectants and Evaluation of
Disinfectant
Unit II Nutrition, cultivation and enumeration of
microorganisms
2.1 Nutritional Requirements : Carbon, Oxygen,
Hydrogen, Nitrogen, Phosphorus, Sulphur and
Growth Factors.
2.2 Classification of Different Nutritional Types of
Organisms.
2.3 Design and Types of Culture Media. Simple Medium,
Differential, Selective and Enriched Media
2.4 Concept of Isolation and Methods of Isolation. Pure
Culture Techniques
2.5 Growth and Enumeration. Growth Phases, Growth
Curve. Arithmatic Growth and Growth Yield.
Measurement of Growth. Chemostat and Turbidostat
2.6 Enumeration of Microorganisms- Direct and Indirect
Methods
2.7 Preservation of Cultures- Principle and
2.8 Methods. Cryogenic Preservation Advantages and
Limitations
15
Lect
ures
Unit III Microscopy and stains
3.1 Microscope- Simple and Compound: Principle.
Parts, Functions and Applications.
3.2 Dark Field and Phase Contrast Microscope
3.3 Stains and Staining Solutions, Definition of Dye
and Chromogen.
3.4 Structure of Dye and Chromophore. Functions of
Mordant and Fixative.
3.5 Natural and Synthetic Dyes.
3.6 Simple Staining, Differential Staining and Acid Fast
Staining with specific examples
15
Lect
ures
Ref:
Microbiology–6th Edition (2006), Pelczar M.J., Chan
E.C.S., Krieg N.R., The McGraw Hill Companies Inc.
NY
Presscott‘s Microbiology, 8th edition (2010), Joanne M
Willey, Joanne Willey, Linda Sherwood, Linda M
Sherwood, Christopher J Woolverton, Chris
Woolverton, McGrawHil Science Enginering, USA
Text book of Medical Microbiology, Anantnarayan
Microbiology- Frobisher
General Principles of Microbiology- Stanier
Fundamental Principles of Bacteriology - A. J. Salle
McGraw Hill
BUSBT105 Biotechnology-I 02
Unit I Scope of Biotechnology
1.1 History & Introduction toBiotechnology
1.2 What is Biotechnology?
1.3 Definition of Biotechnology,
1.4 Traditional and Modern Biotechnology,
1.5 Branches of Biotechnology- Plant, Animal Biotechnology,
Marine Biotechnology, Agriculture, Healthcare, Industrial
Biotechnology, Pharmaceutical Biotechnology,
Environmental Biotechnology.
1.6 Biotechnology Research in India.
1.7 Biotechnology Institutions in India (Public and Private
Sector)
1.8 Biotech Success Stories
1.9 Biotech Policy Initiatives
1.10 Biotechnology in context of Developing World
1.11 Public Perception of Biotechnology
15
Lect
ures
Unit II Applications in Biotechnology
2.1 Applications of Biotechnology in Agriculture : GM
Food, GM Papaya, GM Tomato,
2.2 Fungal and Insect Resistant Plants-BT Crops, BT
Cotton and BT Brinjal. Pros and Cons
2.3 Biotechnological applications in Crop and Livestock
Improvements
2.4 Modifications in Plant Quality-,Golden Rice,
2.5 Molecular Pharming, Plant Based Vaccines
2.6 Ethics in Biotechnology and IPR
15
Lect
ures
Unit III Ecology and interactions
3.1 Ecology and Biogeography. Ecosystems, Definition
and Components, Structure and Function of
Ecosystems.
3.2 Aquatic and Terrestrial Ecosystems,
3.3 Biotic and Abiotic Factors, Trophic Levels, Food
Chain and Food Web,
3.4 Ecological Pyramids (Energy, Biomass and Number)
3.5 Interactions with examples: Commensalism,
Mutualism, Predation and Antibiosis, Parasitism.
15
Lect
ures
Ref:
Biotechnology B.D. Singh.
Biotechnology 3rd Edition S.S. Purohit
Biotechnology ,U.Satyanarayan,Books & Allied
Pvt.Ltd.
Molecular Biotechnology-Principles and Applications
of Recombinant DNA
Technology 3rd Edition Glick B.R., Pasternak J.J.,
Patten C.L
Ecology – P.S. Verma and Agarwaal- S. Chand
Publications
Biotechnology: Environmental Processes- Rehm and
Reed- Wiley
BUSBT106 Biotechnology-II 02
Unit I Mendelian and Non-Mendelian inheritance
1.1 Introduction to Genetics and three branches of
genetics.
1.2 Mendelian inheritance: Terms, Why Pisum sativum,
Mendel’s experimental technique, Mendel’s Laws of
Heredity: Monohybrid cross, Law of Dominance and
Law of Segregation.
1.3 Dihybrid Cross: Law of Independent Assortment.
Application of Mendel’s Principles.
1.4 Mendel’s Principle in Human genetics.
1.5 Non-Mendelian inheritance: Why Mendel was lucky,
existence of Non-Mendelian genetics.
1.6 Incomplete Dominance and Codominance, Multiple
alleles,
1.7 Effect of Environment on the expression of the
genes, Penetrance and expressivity,
1.8 Essential genes and lethal alleles,
1.9 Epistasis (Dominant and recessive) and
Quantitative inheritance (kernel color in wheat).
15
Lect
ures
Unit II Eukaryotic genome and Chromosomal disorders
2.1 Organization of eukaryotic genetic material: Histone
proteins, non-histone proteins, nucleosome structure
and 10 nm fibre, 30 nm fibre (solenoid model and zig-
zag model) and chromosome.
2.2 Kinds of chromosome: Polytene chromosome and
lampbrush chromosome.
2.3 Introduction to cytogenetics: G-Banding and
Karyotyping, Study of normal male and female
karyotype.
15
Lect
ures
Unit III Basic Biostatistics
3.1 Defination &Importance of Statistics in Biology
3.2 Types of Data, Normal and Frequency Distribution
Representation of Data and Graphs(Bar Diagrams, Pie
Charts and Histogram, Polygon and Curve)
3.3 Types of Population Sampling Measures of Central
Tendency (For Raw,Ungroup & Group Data)
3.4 Mean, Median ,Mode. Measures of Dispersion
3.5 Range, Variance, Coefficient of Variance.
3.6 Standard Derivation.Standard Error.
Ref:
Genetics, (2006) Strickberger MW - (Prentice Hall,
India)
Human Genetics- A. M. Winchester – MacMillan Press
Microbial Genetics- Freifelder –Narosa Publishing
House
Genes XI, 11th edition (2012), Benjamin Lewin,
Publisher - Jones and Barlett Inc. USA
Molecular Biology of the Gene, 6th Edition (2008),
James D. Watson, Pearson Education, Inc. and Dorling
Kindersley Publishing, Inc. USA
Molecular Biology, 5th Edition (2011), Weaver R.,
McGraw Hill Science. USA
Fundamentals of Molecular Biology, (2009), Pal J.K.
and Saroj Ghaskadbi, Oxford University Press.
Molecular Biology: genes to proteins, 4th edition
(2011), Burton E Tropp Jones& Bartlett Learning, USA
Cell and Molecular Biology – De Robertis- Lippincott
Williams& Wilkins
Introductory Biostatistics. 1st edition. (2003), Chap T.
Le. John Wiley, USA
Methods in Biostatistics- B. K. Mahajan –Jaypee
Brothers
PRACTICALS
Semester I
BUSBTP101: Basic Chemistry (Credits: 02) 1. Safety Measures and Practices in Chemistry Laboratory
2. Working and use of a Digital Balance, pH Meter
3. Inorganic qualitative analysis of mixtures (1 mixtures)
4. Dissociation Constant of Weak Acids by Incomplete Titration Method using pH
Meter and determination of Acetic acid in Vinegar by Titrimetric Method
5. Determination of strength of HCl in commercial sample
6. Organic qualitative analysis (Identification of functional group and Melting/boiling
point of any 4 organic compound).
7. Determination of Sodium carbonate and bicarbonate by double indicator
8. Preparation of Standard (Molar, Molal and Normal solutions) and Buffer Solutions
9. Spot test for Carbohydrates, Lipids and Nucleic Acids
10. Estimation of Reducing sugar by DNSA method
11. Estimation of DNA by DPA method and RNA by Orcinol method
12. Determination of acid number of oils
13. Determination of iodine value of oils
14. Staining of Starch grain from potato, pea, wheat and rice
BUSBTP102: Basic Life Science (Credits: 02)
1. Study of animal tissues from permanent slides:-blood, bone marrow, neuron,
connective tissue-spongy, smooth, skeletal and heart muscles.
2. Study of plant tissues from suitable material - parenchyma, sclerenchyma, xylem
and phloem.
3. Study of bacterial morphology by Monochrome staining
4. Gram staining of organism from different samples.
5. Study of bacterial structures by staining (capsule, cell wall, lipid granules and
spore).
6. Study of bacterial Motility –hanging drop and stab culture (Demonstration).
7. Preparation of Media- Nutrient broth and Agar, MacConkey Agar, Sabourauds
Agar
8. Aseptic transfer of sterile Nutrient Agar/St. Nutrient broth.
9. Isolation of Organisms : T-streak, Polygon method
10. Colony Characteristics of Microorganisms,
11. Growth Curve of E. coli
BUSBTP103: Biotechnology (Credits: 02) 1. Assignment- Study of any branch of biotechnology and its applications
2. All Lab equipments-autoclave, hot air oven, centrifuge, incubator, rotary shaker, filter
assembly, LAF, pH meter
3. Study of Interactions Commensalism, Mutualism, Predation and Antibiosis, Parasitism
4. Study of all the biotic and abiotic components of any simple ecosystem- natural pond
or terrestrial ecosystem or human modified ecosystem.
5. Problems on Mendelian Genetics
6. Study of Mitosis using Onion root tips
7. Effect of Colchicines on mitosis.
8. Study of Meiosis
9. Study of Karyotypes – Normal Male and Normal Female
10. Isolation of Pectin
11. Extraction of casein
SEMESTER II Credits
BUSBT201 Basic Chemistry- II 02
Unit I Thermodynamics: 1.1 System, Surrounding, Boundaries Sign
Conventions, State Functions, Internal Energy
and Enthalpy: Significance, examples,
1.2 Laws of Thermodynamics and its Limitations,
Mathematical expression.
1.3 Qualitative discussion of Carnot Cycle for ideal
Gas and Mechanical Efficiency.
1.4 Laws of Thermodynamics as applied to
Biochemical Systems.
1.5 Redox Chemistry: Oxidation number, Rules to
assign oxidation number,
1.6 Balancing redox reactions by Ion exchange
method and Oxidation number method.
15
Lectures
Unit II Chemical Kinetics
2.1 Reaction Kinetics: Rate of Reaction, Rate
Constant, Measurement of Reaction Rates
Order & Molecularity of Reaction,
2.2 Integrated Rate Equation of First and Second
order reactions (with equal initial concentration
of reactants).
2.3 Determination of Order of Reaction by
1) Integration Method
2) Graphical Method
3) Ostwald‘s Isolation Method
4) Half Time Method. (Numericals expected).
15
Lectures
Unit III Titrimetry and Gravimetry
3.1 Titrimetric Analysis: Titration, Titrant, Titrand,
End Point, Equivalence Point, Titration Error,
Indicator, Primary and Secondary Standards,
Characteristics and examples
3.2 Types of Titration –Acid –Base, Redox.
Precipitation, Complexometric Titration. Acid –
15
Lectures
Base Titration.-Strong Acid Vs Strong Base -
Theoretical aspects of Titration Curve and End
Point Evaluation.
3.3 Theory of Acid –Base Indicators, Choice and
Suitability of Indicators.
3.4 Gravimetric Analysis: Solubility and
Precipitation, Factors affecting Solubility,
Nucleation, Particle Size, Crystal Growth,
Colloidal State, Ageing/Digestion of
Precipitate.
3.5 Co-Precipitation and Post-Precipitation.
Washing, Drying and Ignition of Precipitate.
Ref:
University General Chemistry , 1st edition
(2000), C.N. R. Rao, Macmillan Publishers, India
,
Physical Chemistry University for biological
sciences, 1st edition, (2005), Chang R., Science
Books, USA
Essentials of Physical Chemistry, 24th edition,
(2000), B S Bahl, G D Tuli, Arun Bahl, S. Chand
Limited, India.
Concise Inorganic Chemistry .5th edition (2008),
Author: J. D. Lee, John Wiley & Sons, USA.
Organic Chemistry, 6th edition, (1992), Morrison
Robert Thornton, Pearson Publication, Dorling
Kindersley (India Pvt. Ltd.)
BUSBT202 Biochemistry- II 02
Unit I Amino acids and proteins
1.1 Proteins and Amino Acids: Classification,
Preparation and Properties, Isoelectric Point,
Peptide Synthesis
1.2 Proteins: Classification based on Structure and
Functions, Primary Structure, N-terminal (Sanger
and Edmans Method) and C-terminal Analysis
(Enzyme)Reactions of Amino Acids, Sorenson‘s
Titration, Ninhydrin Test.
1.3 Denaturation of protein Structure of Peptides.
1.4 Titration Curve of Amino Acids.
1.5 Concept of Isoelectric pH, Zwitter ion.
Glycoproteins
15
Lectures
Unit II Enzymes, Coenzymes and Vitamins
2.1 Enzymes: Introduction, Nomenclature and
classification. Chemical nature and
15
Lectures
properties. Mechanism of enzyme
action/catalysis (introduction)
2.2 Active site, Isozymes, Ribozymes and
Abzymes
Co factor and Co-enzyme
2.3 Vitamins and Coenzymes: RDA, source,
structure (of Vitamin and Coenzyme) and
functions of:
1 Water soluble Vitamins: Ascorbic acid,
thiamine, riboflavin, folic acid, pyridoxine,
niacin, pantothenic acid, biotin, lipoic , folic
acid and cyanocobalamin.
2 Fat soluble Vitamins: Vitamin A, D, E and K.
Unit III Analytical techniques
3.1 Methods of Separation: Precipitation, Filtration,
Distillation and Solvent Extraction.
3.2 Analytical Techniques: Chromatography:
Definition, Principles, Types
3.3 Introduction to Paper Chromatography, Thin
Layer Chromatography, Column
Chromatography and its Applications.
3.4 Colorimetry: Principle, Beer-Lambert‘s Law,
Measurement of Extinction, Derivation of E =
kcl, Limitations of Beer-Lambart‘s Law, Filter
Selection
15
Lectures
Ref:
Outlines of Biochemistry: 5th Edition, (2009),
Erice Conn & Paul Stumpf ; John Wiley and
Sons, USA
Principles of Biochemistry, 4th edition (1997),
Jeffory Zubey, McGraw-Hill College, USA
Lehninger , Principles of Biochemistry. 5th
Edition (2008), David Nelson & Michael Cox,
W.H. Freeman and company, NY.
Fundamentals of Biochemistry. 3rd Edition
(2008), Donald Voet & Judith Voet , John Wiley
and Sons, Inc. USA
Biochemistry: 7th Edition, (2012), Jeremy Berg,
Lubert Stryer, W.H.Freeman and company, NY
Enzymes: Biochemistry, Biotechnology &
Clinical chemistry, (2001) Palmer Trevor,
Publisher: Horwood Pub. Co., England
Principles and techniques in biochemistry and
molecular biology (2010), Keith
Wilson and John Walker, 7th edition, Cambridge
University Press
BUSBT203 Basic Life Science-I 02
Unit I Prokaryotic Cell Organization and Division
1.1 Ultrastructure of Prokaryotic Cell Size, shape and
arrangements in Bacteria. Cell envelopes: Cell
membrane and Cell wall (Gram positive, Gram
negative and Archaebacteria), Slime Layer and
Capsule.
1.2 Cell surface Structures: Pili and fimbriae, Flagella and
Microbial locomotion
1.3 Cytoplasm :Organization of Genetic Material,
plasmids (Features and Classification) , Storage
1.4 Bodies, Endospores (structure, sporulation and
germination)
1.5 Forms of Bacterial cell Division and Growth phases
(introduction)
15
Lectures
Unit II Eukaryotic Cell Organization and Division
2.1 Ultrastructure of Eukaryotic Cell: Structure and
function of: Plasma membrane, plant cell wall,
Nucleus, Mitochondria, Plastids and Chloroplast,
Endoplasmic Reticulum, Golgi Apparatus, Lysosome,
and Ribosomes.
2.2 Comparison of Prokaryotic And Eukaryotic Cells.
2.3 Introduction to Microfilaments, Intermediate
Filaments, and Microtubules. Locomotory
appendages: Cilia And Flagella. Cell division
(Mitosis).
15
Lectures
Unit III Classification of Viruses
3.1 General Characters, Classification- Plant,
Animal and Bacterial Viruses
3.2 Structure of viruses- bacteriophage, animal
viruses- Small pox , Influenza, Plant viruses-
TMV, CMV
3.3 Structure and Characterization of Viruses and
Significance
3.4 Classification and taxonomy.
15
Lectures
Ref:
Microbiology–6th Edition (2006), Pelczar M.J.,
Chan E.C.S., Krieg N.R., The McGraw Hill
Companies Inc. NY
Presscott‘s Microbiology, 8th edition (2010),
Joanne M Willey, Joanne Willey, Linda
Sherwood, Linda M Sherwood, Christopher J
Woolverton, Chris Woolverton, Mc Graw Hill
Science Engineering, USA
Text book of Medical Microbiology,
Anantnarayan
Microbiology- Frobisher
General Principles of Microbiology- Stanier
Fundamental Principles of Bacteriology - A. J.
Salle McGraw Hill
Foundations In Microbiology by Talaro and
Talaro Third edition W.C Brown
Cell and Molecular Biology – De Robertis-
Lippincott Williams& Wilkins Cell and
Molecular Biology- Concepts and Experiments—
Karp – Wiley International
BUSBT204 Basic Life Science-II 02
Unit I Plant Physiology
1.1 Photosynthesis, Intracellular Organization of
Photosynthetic System.
1.2 Fundamental Reactions of Photosynthesis,
Photosynthetic Pigments, Role of Light. Hill
Reaction and its Significance, Light Reactions,
1.3 Cyclic and Non-Cyclic Photo induced Electron
Flow,
1.4 Energetics of Photosynthesis, Photorespiration,
Dark Phase of Photosynthesis, Calvin Cycle, C-
3, C-4 pathways
1.5 Plant hormones - Auxin ,Gibbrellins,
Cytokinins, Ethylene, Abscissic acid
1.6 Introduction to Secondary Metabolites
15
Lectures
Unit II Animal Physiology
2.1 Physiology of Digestion Movement of Food and
Absorption, Secretory functions of Alimentary
Canal, Digestion and Absorption,assimilation in
Gut of Mammals
2.2 Physiology of Urine Formation and Role of
Kidney in Excretion and Osmoregulation
2.3 Physiology of Respiration, Mechanism of
Respiration, Principles of Gaseous Exchange in
the Blood and Body Fluids
2.4 Blood and Circulation : Blood Composition,
Structure and Function of its Constituents,
2.5 Blood Coagulation and Anti-Coagulants
15
Lectures
Unit III Nutrient Cycle and Biogeochemical Cycles
3.1 Water, Carbon, Oxygen, Nitrogen, Sulphur and
15
Lectures
Phosphorus.
3.2 Components, Significance, Disruption of the
ecosystem due to anthropological activities.
3.3 Concept of - Endangered, Threatened,
Vulnerable, Rare and Extinct species
Ref:
Jordan, E.L. and Verma P.S. 1978, (i) Chordate
Zoology S. Chand & Company Ltd. Ram Nagar.
New Delhi.
Modern Text Book of Zoology: Invertebrates.,
R.L.Kotpal. Publisher, Rastogi Publications,
2012.
Guyton, Text book of Medical Physiology
Concise Medical Physiology- Sujit K Chaudhari
Human Physiology- Guyton –International
Edition
Human Anatomy- Marieb
Devlin R.M. (1983) - Fundamentals of Plant
Physiology (Mac. Millan, New York)
Dutta A.C. (2000) A Classbook of Botany
(Oxford University Press, UK)
Ganguli, Das Dutta (2011) – College Botany Vol
I, II and III (New Central Book Agency, Kolkata)
Ecology – P.S. Verma and Agarwaal- S. Chand
Publications
Microbiology–6th Edition (2006), Pelczar M.J.,
Chan E.C.S., Krieg N.R., The McGraw Hill
Companies Inc. NY
Prescott‘s Microbiology, 8th edition (2010),
Joanne M Willey, Joanne Willey, Linda
Sherwood, Linda M Sherwood, Christopher J
Woolverton, Chris Woolverton, McGrawHil
Science Enginering, USA
BUSBT205 Biotechnology-I 02
Unit I Immunology
1.1 Innate and acquired immunity, Organs of
immune system :
1.2 First, second and third line of defense.
Mechanism of innate immune response.
1.3 Natural - Active and passive immunity.
1.4 Artificial immunity - Active and passive
immunity.
1.5 Organs of immune system – primary lymphoid
organs, secondary lymphoid organs.
15
Lectures
Unit II Microbial genetics
2.1 Genetic analysis in Bacteria- Prototrophs,
Auxotrophs.
2.2 Bacteriophages: Lytic and Lysogenic
Development of Phage.
2.3 Mechanism of Genetic Exchange in Bacteria:
Conjugation; Transformation; Transduction;
(Generalized Transduction, Specialized
Transduction)
15
Lectures
Unit III Population genetics
3.1 Genetic Structure of Populations – Genotypic
Frequencies and Allelic Frequencies, Hardy-
Weinberg Law and its assumptions.
3.2 Genetic Variations in Populations- Measuring
Genetic Variation at Protein Level and
measuring Genetic
3.3 Variations at DNA level, Natural Selection.
Genetic Drift. Speciation .
3.4 Role of Population Genetics in Consevation
Biology
15
Lectures
Ref:
Genetics, (2006) Strickberger MW - (Prentice
Hall, India)
Human Genetics- A. M. Winchester – MacMillan
Press
Microbial Genetics- Freifelder –Narosa
Publishing House
Kuby immunology, Judy Owen , Jenni Punt ,
Sharon Stranford., 7th edition (2012), Freeman
and Co., NY
Textbook of basic and clinical immunology, 1st
edition (2013), Sudha Gangal and Shubhangi
Sontakke, University Press, India
Immunology, 7th edition (2006), David Male,
Jonathan Brostoff, David Roth, Ivan Roitt,
Mosby, USA.
Introduction to Immunology- C V Rao- Narosa
Publishing House
BUSBT206 Biotechnology-II 02
Unit I Model organisms in biology
1.1 Define model organisms, Concept of Animal
models, Types of animal models.
1.2 Criteria for selection of model system
1.3 Prokaryotic: E. coli and its maintenance
15
Lectures
1.4 Eukaryotic: i. Fungi: Saccharomyces
cerevisiae, and Neurospora crassa
1.5 Plantae: Zea mays and Pisum sativum
1.6 Animals: Albino mice, Guinea pigs, Hamsters,
Monkey, etc, and their maintenance.
1.7 Drosophila and its maintenance
Unit II Prokaryotic and Eukaryotic Replication
2.1 DNA Replication in Prokaryotes and
Eukaryotes- Semi-conservative DNA replication,
2.2 DNA Polymerases and its role,
2.3 E.coli Chromosome Replication,
2.4 Bidirectional Replication of Circular DNA
molecules. Rolling Circle Replication,
2.5 DNA Replication in Eukaryotes
2.6 DNA Recombination – Holliday Model for
Recombination Transformation
Unit III DNA mutations and DNA repair
3.1 Definition and Types of Mutations.
3.2 Mutagenesis and Mutagens.( Examples of
Physical, Chemical and Biological Mutagens)
Types of Point Mutations,
3.3 DNA repair - Photoreversal, Base Excision
Repair, Nucleotide Excision Repair, Mismatch
Repair, SOS Repair and Recombination Repair.
15
Lectures
Ref:
Genes XI, 11th edition (2012), Benjamin Lewin,
Publisher - Jones and Barlett Inc. USA
Molecular Biology of the Gene, 6th Edition
(2008), James D. Watson, Pearson Education,
Inc. and Dorling Kindersley Publishing, Inc. USA
Molecular Biology, 5th Edition (2011), Weaver
R., McGraw Hill Science. USA
Fundamentals of Molecular Biology, (2009), Pal
J.K. and Saroj Ghaskadbi, Oxford University
Press.
Molecular Biology: genes to proteins, 4th edition
(2011), Burton E Tropp Jones& Bartlett
Learning, USA
iGenetics A Molecular Approach 3rd Edition
Peter J. Russell.
Molecular Biotechnology-Principles and
Applications of Recombinant DNA Technology
3rd Edition Glick B.R., Pasternak J.J., Patten C.L.
PRACTICALS
Semester II
BUSBTP201: Basic Chemistry (Credits: 02)
1. To determine enthalpy of dissolution of salt like KNO3
2. Determine the rate constant for hydrolysis of ester using HCl as a catalyst
3. Study the kinetics of reaction between Thiosulphate ion and HCl
4. Study reaction between potassium Persulphate and Potassium Iodide kinetically and
hence to determine order of reaction
5. Determination of amount of K oxalate and oxalic acid in the given solution
Titrimetrcially
6. Determination of percent composition of BaSO4 and NH4Cl in the given mixture
Gravimetrically.
7. Qualitative detection of amino acids and proteins.
8. Colorimetric estimation of proteins by Biuret method.
9. Estimation of Vitamin C by Iodometric titration.
10. Paper chromatography of amino acids and sugars.
11. Thin layer chromatography of lipids.
BUSBTP202: Life Sciences (Credits: 02)
1. Permanent slides of - Nostoc, Anabaena, Oscillatoria, Rivularia.
2. Study of Mammalian Blood-
A. Blood count (WBC and RBC) using Haemocytometer
B. Polychrome staining
3. Study of Human Blood Groups
4. Study of Mammalian Kidney and Heart (Permanent slides)
5. Soil studies (Physical characteristics of soil-acidity, alkalinity, pH).
6. To estimate Dissolved oxygen in a given water sample
7. Study of Air Flora and colony character.
BUSBTP203: Biotechnology (Credits: 02)
1. Effect of temperature, pH and osmotic pressure on cell
2. Study of Drosophila.
3. Enumeration of viable microorganisms by
a. Serial Dilution,
b. Pour Plate,
c. Spread Plate Method
4.Enumeration by
a. Breed‘s count,
b. Brown's Opacity tubes
5.Industrial Visit and report.
6. Qualitative assay of enzymes (Amylase, Urease, Dehydrogenase, catalase
7. Enzyme Kinetics: effect of pH, temperature, Subtract concentration , Inhibitors – on
enzyme.
Semester – I and II
Ability Enhancement Course 1 (FC I)
Ability Enhancement Course 2 (FC II)
Course Code Title Credits
USBT 107 Societal Awareness 02
Unit I
Overview of Indian Society 1.1 Understand the multi-cultural diversity of Indian
society through its demographic composition:
1.2 Population distribution according to religion, caste,
and gender;
1.3 Appreciate the concept of linguistic diversity in
relation to the Indian situation;
1.4 Understand regional variations according to rural,
urban and tribal characteristics;
1.5 Understanding the concept of diversity as difference
15
Lectures
Unit II
Concept of Disparity- I 2.1 Understand the concept of disparity as arising out of
stratification and inequality;
2.2 Explore the disparities arising out of gender with
special reference to violence against women, female
foeticide (declining sex ratio), and portrayal of
women in media;
2.3 Appreciate the inequalities faced by people with
disabilities and understand the issues of people with
physical and mental disabilities
2.4 Concept of Disparity-II Examine inequalities
manifested due to the caste system and inter-group
conflicts arising thereof;
2.5 Understand inter-group conflicts arising out of
communalism;
2.6 Examine the causes and effects of conflicts arising out
of regionalism and linguistic differences
15
Lectures
Unit III
The Indian Constitution 3.1 Philosophy of the Constitution as set out in the
Preamble;
3.2 The structure of the Constitution-the Preamble, Main
Body and Schedules;
3.3 Fundamental Duties of the Indian Citizen; tolerance,
peace and communal harmony as crucial values in
strengthening the social fabric of Indian society;
3.4 Basic features of the Constitution
3.5 Significant Aspects of Political Processes he party
system in Indian politics;
3.6 Local self-government in urban and rural areas; the
73rd and 74th Amendments and their implications
for inclusive politics;
3.7 Role and significance of women in politics
15
Lectures
Topics for Project Guidance: Growing Social Problems in India: 1. Substance abuse‐ impact on youth & challenges for the future
2. HIV/AIDS‐ awareness, prevention, treatment and services
3. Problems of the elderly‐ causes, implications and response
4. Issue of child labour‐ magnitude, causes, effects and response
5. Child abuse‐ effects and ways to prevent
6. Trafficking of women‐ causes, effects and response
Course Code Title Credits
USBT 207 Globalization, Ecology and Sustainable Development
02
Unit I
Globalisation and Indian Society
1.1 Understanding the concepts of liberalization,
privatization and globalization;
1.2 Growth of information technology and
communication and its impact manifested in everyday
life;
1.3 Impact of globalization on industry: changes in
employment and increasing migration;
1.4 Changes in agrarian sector due to globalization; rise
in corporate farming and increase in farmers’
suicides.
1.5 Human Rights Concept of Human Rights; origin and
evolution of the concept;
1.6 The Universal Declaration of Human Rights;Human
Rights constituents with special reference to
Fundamental Rights stated in the Constitution
15
Lectures
Unit II
Ecologyand Sustainbale Development 2.1 Importance of Environment Studies in the current
developmental context;
2.2 Understanding concepts of Environment, Ecology
and their interconnectedness;
2.3 Environment as natural capital and connection to
quality of human life;
2.4 Environmental Degradation causes and impact on
human life; Sustainable development, concept and
components; poverty and environment
15
Lectures
Unit III
Understanding Stress and Conflict 3.1 Causes of stress and conflict in individuals and
society;
3.2 Agents of socialization and the role played by them in
developing the individual;
3.3 Significance of values, ethics and prejudices in
developing the individual;
3.4 Stereotyping and prejudice as significant factors in
15
Lectures
causing conflicts in society.
3.5 Aggression and violence as the public expression of
conflict
3.6 Managing Stress and Conflict in Society Types of
conflicts and use of coping mechanisms for
managing individual stress;
3.7 Maslow’s theory of self‐actualisation;Different
methods of responding to conflicts in society;
3.8 Conflict‐resolution and efforts towards building peace
and harmony in society
Topics for Project Guidance: Growing Social Problems in India:
1. Increasing urbanization, problems of housing, health and sanitation;
2. Changing lifestyles and impact on culture.
3. Farmers’ suicides and agrarian distress.
4. Debate regarding Genetically Modified Crops.
5. Development projects and Human Rights violations.
6. Increasing crime/suicides among youth.