FUNGAL FLORA ASSOCIATED WITH SEEDS OF JATHROPA CURCAS AND THEIR EFFECTS ON SEED QUALITY

AND OIL YIELD

• Teresita U. Dalisay (Crop Protection Cluster, UPLB-CA

• Laura J. Pham (BOTECH, UPLB)

FUNGAL FLORA ASSOCIATED WITH SEEDS OF TUBA-TUBA (JATROPHA CURCAS L.) AND THEIR EFFECTS ON GERMINATION, SEEDLING STAND,

QUALITY AND QUANTITY OF OIL

TERESITA U. DALISAY (Crop Protection Cluster, UPLB-CA)LAURA J. PHAM (Biotech, UPLB)

CELYNNE R. OCAMPO (Undergrad. student, Crop Protection Cluster, UPLB-CA)

FUNGAL FLORA ASSOCIATED WITH SEEDS OF JATHROPA CURCAS AND THEIR EFFECTS ON SEED QUALITY

AND OIL YIELD

• Teresita U. Dalisay (Crop Protection Cluster, UPLB-CA

• Laura J. Pham (BOTECH, UPLB)

INTRODUCTION

OBJECTIVES

To identify fungi in seeds of Jatropha

To determine the effect of the seed-borne

fungi on germination and seedling stand

To evaluate quantity and quality of oil

as affected by the seed-borne fungi

METHODOLOGY

Objective 1: to identify fungi in seeds of Jatropha

Disinfested with 10% NaOCl for 3 minutes

Washed in changes of sterile distilled water

Plated unto petri plates lined with wet blotter paper

RESULTS

Site 1Gen. Santos

Site 2Gen. Santos

Davao PNOCMindanao

Site 1South

Cotabato

Site 2South Cotabato

TOTAL FUNGI

50

14

Seed-borne fungi

E. F.

E.

Seed-borne Fungi

Penicillium

Neofusicoccum

percent occurrence

0 0 0 0 0 0

999

73

0

10

20

30

40

50

60

70

80

Asp

ergillu

s

Penicillium

Fusarium

Cha

etom

ium

Nigrosp

ora

Las

iodiplod

ia

Alte

rnaria

Clado

spor

ium

Peyr

onellaea

Sterile myc

elia

FUNGAL ISOLATES FROM GEN. SANTOS (SITE 1)

% O

CC

UR

RE

NC

E

0 0 0 0 0

9

64

9 9 9

0

10

20

30

40

50

60

70

Asp

ergi

llus

Peni

cilli

um

Fusa

rium

Cha

etom

ium

Nig

rosp

ora

Las

iodi

plod

ia

Alte

rnar

ia

Clado

spor

ium

Peyr

onellaea

Sterile m

ycelia

FUNGAL ISOLATES FROM GEN. SANTOS (SITE 2)

% O

CC

UR

RE

NC

E

50

0 0 0 0 0

21

77

14

0

10

20

30

40

50

60

Asp

ergi

llus

Peni

cilli

um

Fusa

rium

Cha

etom

ium

Nig

rosp

ora

Las

iodi

plod

ia

Alte

rnar

ia

Clado

spor

ium

Peyr

onellaea

Sterile m

ycelia

FUNGAL ISOLATES FROM DAVAO

% O

CC

UR

RE

NC

E

0 0 0 0 0

999

18

54

0

10

20

30

40

50

60

Asp

ergi

llus

Peni

cilli

um

Fusa

rium

Cha

etom

ium

Nig

rosp

ora

Lasiodi

plod

ia

Alte

rnar

ia

Clado

spor

ium

Peyr

onellaea

Sterile

myc

elia

FUNGAL ISOLATES FROM PNOC, MINDANAO

% O

CC

UR

RE

NC

E

0 0 0 0

45

9 9 9 9

18

0

10

20

30

40

50

Asp

ergi

llus

Peni

cilli

um

Fusa

rium

Cha

etom

ium

Nig

rosp

ora

Lasiodi

plod

ia

Alte

rnar

ia

Clado

spor

ium

Peyr

onellaea

Sterile

myc

elia

FUNGAL ISOLATES FROM SOUTH COTABATO (SITE 1)

% O

CC

UR

RE

NC

E

0 0 0 0 0

9

64

9 9 9

0

10

20

30

40

50

60

70

Asp

ergi

llus

Peni

cilli

um

Fusa

rium

Cha

etom

ium

Nig

rosp

ora

Lasiodi

plod

ia

Alte

rnar

ia

Clado

spor

ium

Peyr

onellaea

Sterile

myc

elia

FUNGAL ISOLATES FROM GEN. SANTOS (SITE 2)

% O

CC

UR

RE

NC

E

Fungal genera harbored from seeds

54.54

18.18

6.063.03 3.03

6.063.03 3.03 3.03

0

10

20

30

40

50

60

Asp

ergillu

s

Penicillium

Fusa

rium

Cha

etom

ium

Nig

rosp

ora

Las

iodiplod

ia

Alterna

ria

Clado

sporiu

m

Peyr

onellaea

Genus composition

Percen

t p

ro

po

rti

on

Objective 2: To determine effect of

seed-borne fungi on germination

and seedling stand of Jatropha

Effect on Germination

52

70

50

4246

2026

40

68

48

0

10

20

30

40

50

60

70

80

Asp

sp.

CRO

1

Asp

sp.

CRO

7

Fus sp

.CRO

11

Asp

sp.

CRO

12

Asp

sp.

CRO

13

Pen

sp.C

RO21

Nig

sp.

CRO30

Cla sp.

CRO33

Pey

sp.C

RO14

Con

trol

FUNGAL ISOLATES

% G

ER

MIN

AT

IO

N

a

abc

abcdabcd

dcd

bcd

ab

abc abc

Effect on Plant Height

15.96

13.07

21.16

9.45

5.795

12.2011.28

14.83

21.41

0

5

10

15

20

25

Asp

sp.C

RO1

Asp

sp.C

RO7

Fus s

p.CRO

11

Asp

sp.C

RO12

Asp

sp.C

RO13

Pen

sp.C

RO21

Nig

sp.C

RO30

Cla sp

.CRO33

Pey

sp.C

RO14

Con

trol

FUNGAL ISOLATES

PL

AN

T H

EIG

HT

(cm

)

a

ab

bcdbcd

d cd

bcd

a

bc

ab

Effect of Root Length

5.33

4.38

3.59

6.00

2.76

1.671.57

3.603.27

3.94

0

1

2

3

4

5

6

7

Asp

sp.C

RO1

Asp

sp.C

RO7

Fus s

p.CRO

11

Asp

sp.C

RO12

Asp

sp.C

RO13

Pen

sp.C

RO21

Nig

sp.C

RO30

Cla sp

.CRO33

Pey

sp.C

RO14

Con

trol

FUNGAL ISOLATES

RO

OT

LE

NG

TH

(cm

)

abc

bcd bcd

d d

cd

a

abc

ab

bcd

Aspergillus sp. CRO12

Aspergillus sp. CRO13

Objective 3: To evaluate quantity

and quality of oil as affected by the

seed-borne fungi

27.60

24.1226.19

27.34

24.1224.2426.31

0

5

10

15

20

25

30

Asp

sp.CRO 1

Asp

sp.CRO 7

Fus sp

.CRO 11

Asp

sp.CRO 12

Asp

sp.CRO 13

Pen sp

.CRO 21

Con

trol

FUNGAL ISOLATES

%O

IL

YIE

LD

a a a

a a

a a

27.54

23.9223.5426.22

23.9422.02

23.81

0

5

10

15

20

25

30

Asp

sp.CRO 1

Asp

sp.CRO 7

Fus sp

.CRO 11

Asp

sp.CRO 12

Asp

sp.CRO 13

Pen sp

.CRO 21

Con

trol

FUNGAL ISOLATES

% O

IL

YIE

LD

ab b

ab ab ab ab

a

23.42

18.19

23.6125.91

23.36 22.87

26

0

5

10

15

20

25

30

Asp

sp.CRO 1

Asp

sp.CRO 7

Fus sp

.CRO 11

Asp

sp.CRO 12

Asp

sp.CRO 13

Pen sp

.CRO 21

Con

trol

FUNGAL ISOLATES

% O

IL

YIE

LD

b

a a

a a

a ab

2nd wk of incubation 3rd wk of incubation

4th week of incubation

2nd week of incubation 3rd week of incubation

4th week of incubation

Dominant fungi harbored on seeds of Jatropha are species of Aspergillus and Penicillium with 54.5% and 18.8% occurrences, respectively.Seeds inoculated with Aspergillus sp.CRO12 and CRO13 resulted to 20-40% germination, about 15-20% reduction in seedling height and root length.Aspergillus sp. CRO7 caused 12.17%

reduction in oil during the second week of storage, 22.04% and 30.14% for the third and fourth week, respectively.

CONCLUSIONS