INNOVATIVE INGREDIENTS

Functional IngredientsTRICOREXINA HYDRO®BOTANICAL COMPLEX ACTIVE- HAIRCARE BEAUTY

PREVENTS HAIR LOSS

REJUVINATES THE SCALP

GROWS THICKER, FULLER HAIR

STIMULATES HAIR FOLLICLE DERMAL PAPILLA CELLS

BENEFITS

HAIR SERUM

HAIR MIST

HAIR ESSENCE

HAIR PACK

CONDITIONER

MOUSTACHE BOTANICAL COSMETICS

APPLICATIONS

COMPLEX OF WATER SOLUBLE BOTANICAL EXTRACTS THAT WORK TOGETHER TO HELP REDUCE HAIR LOSS

Tricorexina® Hydro is a complex of water-soluble botanical extracts whose synergy helps prevent hair loss. The multifunctional botanical extracts of Tussilago Butterfara, Achillea Millefolium and Cinchona Berries, acts as active ingredients in Tricorexina® Hydro and are at the basis for the development of products for the health and wellbeing of the scalp.

PROVEN ACTIVITYStimulates keratinocyte cell growth and proliferation (in vitro).Stimulates protein metabolism in fibroblasts (in vitro).Stops oxidative stress and acts as anti-oxidant in keratinocytes (in vitro).

Decreases hair loss (by 78.00%)*Reduces itching of the scalp (-48.40%)*Reduces flakiness of the scalp (-37.40%)*Reduces sebum production (-39.00%)*

*in vivo study conducted in 20 healthy volunteers between 18 and 70 years of age. Volunteers used the cosmetic product (containing 10% Tricorexina Hydro) twice a week for 56 days.

EFFICACY ON CELL PROLIFERATION AND TURNOVER IN HUMAN KERATINOCYTES

Dermal papilla cells are located inside the hair follicle and play an essential role in controlling hair growth. These cells play a crucial role not only in physiological hair growth but also in certain conditions, such as androgenetic alopecia.Results show a stimulation of the metabolism of keratinocytes with an increase in the expression of keratin.

in vitro test

40 36

28 27 26 25

1614

12,5

TRICOREXINA HYDRO mg/ml

% C

ELL

VIT

ALI

TY IN

DU

CTI

ON

30

20

10

06,25 1,560 0,78 0,390 0,1953,125

TRICOREXINA® HYDROTussilago Butterfara (Coltsfoot) Leaf Extract, Achillea Millefolium Flower Extract, Cinchona Succirubra Bark Extract, Sodium Benzoate, Potassium Sorbate

in vitro test

IN VITRO TEST

ANTIOXIDANT EFFICACY ON HUMAN KERATINOCYTES

ANTIOXIDANT ACTION OF TRICOREXINA HYDRO ON HUMAN KERATINOCYTES

ANTIOXIDANT ACTIVITY

%FR

EE R

AD

ICA

L IN

HIB

ITIO

N

UVA IRRADIATED J/cm2

30

1 mg/ml 22,8

13,8 15,4 2,3

10J/cm2 UVA 15J/cm2 UVA 20J/cm2 UVA

0,1 mg/ml

25

20

15

10

5

0

25,6 16,2

ANTIOXIDANT ACTION OF VITAMIN C ON HUMAN KERATINOCYTES

ANTIOXIDANT ACTIVITY

%FR

EE R

AD

ICA

L IN

HIB

ITIO

N

UVA IRRADIATED J/cm2

30

1 mg/ml 15,3

12,8 3,8 0,2

10J/cm2 UVA 15J/cm2 UVA 20J/cm2 UVA

0,1 mg/ml

25

20

15

10

5

0

0,8 0,5

Pre-treated human keratinocytes show an increased ability to counteract free radical formation induced by subsequent exposure to UVA irradiation. The two concentrations tested showed an excellent ability to inactivate free radicals. Data evaluation was obtained by comparing results with those of a known antioxidant, Vitamin C.Results show that TRICOREXINA ® Hydro has a higher antioxidant efficacy than Vitamin C, even at high doses of irradiation.

By fighting oxidative stress, TRICOREXINA ® Hydro protects the energy metabolism, thus guaranteeing the correct nutritional contribution. Furthermore, by limiting the damage caused by oxidative stress, TRICOREXINA ® Hydro has a soothing effect and helps prevent ageing.

in vitro test

IN VITRO TEST

TRICOREXINA ® Hydro induces a significant increase in cell viability at the tested concentrations, with a peak activity at the dose of 12.5 mg/mL (after 72 hours). Its effectiveness can be further proven even at the maximum dilution where it promotes stimulation by more than 14% .

TRICOREXINA ® Hydro stimulates keratinocyte growth and therefore their production of keratin and in turn hair growth. (Keratinocytes stimulate melanocyte functions such as proliferation, differentiation, melanogenesis, and dendritogenesis. A function of keratinocytes is to produce keratin and filaggrin, which are involved in regulating the barrier function.)

During skin ageing, collagen content is markedly reduced. As a result, the strength of the dermal papilla cells in the hair follicles of the scalp decreases and this promotes hair loss. The effect of TRICOREXINA ® Hydro on protein expression has been evaluated in human fibroblasts:

The cells were treated with TRICOREXINA ® Hydro at intervals of 24, 48 and 72 hours. At the end of each treatment protein synthesis was evaluated. TRICOREXINA ® Hydro stimulates protein metabolism in each treatment, even at maximum dilution, with a peak activity at 24 hours, and an increase of 140% to 1.6 mg/ml.TRICOREXINA ® Hydro stimulates fibroblast protein metabolism, reinforcing the dermal structures on the scalp and slowing hair loss.

EFFICACY ON PROTEIN METABOLISM IN HUMAN FIBROBLASTS

in vitro test

%TO

TAL

PR

OTE

IN IN

DU

CTI

ON

%TRICOREXINA HYDRO mg/ml

140

24 HRS

48 HRS

72 HRS

120

100

80

60

40

20

012,5

57

24

0

6,25

61

20

17

3,125

63

20,3

11

1,563

140

17

26

0,78

70,7

19

10

A clinical study on a hair lotion containing 10% TRICOREXINA ® Hydro in order to evaluate the cosmetic activity related to the prevention of hair loss. The study was conducted on 20 healthy volunteers, 12 women and 8 men, aged 18 to 70. Volunteers used the product for 56 days, applying it twice a week.

The following parameters were assessed:

FORMULATION GUIDELINESRecommended concentration levels for use at 5.00-15.00%. Water soluble. For cold/hot process, add to the water phase at room temperature or below 40°C during the cooling phase.Physical appearance: yellow-brown fluid liquid, easy to incorporate into any formulation.

18PS32 HAIRSHINE MASK

INCI Name % Activity Supplier

Phase A

Aqua TO 100 Solvent

RESASSOL® DN Diglycerin 2.00 Humectant RES PHARMAINDUSTRIALE

Phase B

EMULPHARMA® COREOSOME

Cetearyl Glucoside, Sorbitan Stearate, Glyceryl Stearate,

Cetearyl Alcohol7.00 COSMOS Oleosome Promoter Emulsifier

RES PHARMAINDUSTRIALE

EMULPHARMA® ECO10 Polyglyceryl-10 Laurate 1.80 COSMOS PGF Emulsifier RES PHARMAINDUSTRIALE

Macadamia Ternifolia Seed Oil 3.00 Vegetable Oil

Behentrimonium Chloride 3.50 Cationic Surfactant

Tocopheryl Acetate 0.30 Antioxidant

Phase C

TRICOREXINA® HYDRO

Tussilago Farfara Extract, Achillea Millefolium Extract,

Cinchona Succirubra Extract, Sodium Benzoate, Potassium Sorbate

2.00 Water Soluble Vegetable ExtractsRES PHARMAINDUSTRIALE

Phase D

PRESERVATIVE PBDE®Phenoxyethanol, Benzoic Acid,

Dehydroacetic Acid, Ethylhexylglycerin

1.20 Preservative RES PHARMAINDUSTRIALE

Phase E

Parfum 0.30 Fragrance

HAIR CARE WITH PROVEN IN VIVO EFFICACY

BOTANICAL COSMOS APPROVED COMPLEX

SUSTAINABLE ACTIVE REBOOTING

*self evaluation - scale from 0 to 10

SENSORIAL PARAMETERS % PERCEIVED EFFICACYafter 56 days of treatment

PEELING OF TH SCALP SCALP

ITCHING OF THA SCALP

HAIR OILYNESS

RAPIDITY OF ABSORPTION/DRYING

37,4%

48,4%

39,0%

7,15%*

in vivo test

SELF - EVALUATION

MARKETING BENEFITS

RES PHARMA INDUSTRIALE SRL - Via G. Pastore, 3 - 20056 Trezzo s/Adda, Milano - ITALY T +39.02.90 99 41 - info@respharmaind.com - www.respharmaind.com

INNOVATIVE INGREDIENTS

The data provided in this publication is based on our current knowledge and experience and is offered free of charge and in good faith for guidance only. It does not constitute a guarantee in the legal sense of the term and in view of the multiple factors that may affect processing and applications, it does not relieve those to whom we supply our products from the responsibility of carrying out their own tests and experiments. All express and implied warranties are disclaimed. Any relevant patent rights and existing legislations and regulations must be observed.tri

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