fun with volume measurement
DESCRIPTION
Fun with Volume Measurement. Ellyn Daugherty SM Biotech Career Pathway www.BiotechEd.com www.emcp.com/biotech [email protected] www.sargentwelch.com/biotech. Text - Chapter 3 Lab Manual - Chapter 3 Student CD Tutorials Course Planner. Volume Measurement in Biotech 1 Chapter 3. - PowerPoint PPT PresentationTRANSCRIPT
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Ellyn DaughertySM Biotech Career Pathway
www.BiotechEd.com
www.emcp.com/biotech
www.sargentwelch.com/biotech
• Text - Chapter 3• Lab Manual - Chapter 3• Student CD Tutorials• Course Planner
Fun with Volume Measurement
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Volume Measurement in Biotech 1 Chapter 3
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On all instruments:
• What is the total volume that can be measured?
• What is the value of each type of graduation?
Start with Graduated Cylinders
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• L mL µL
Review: Metric Measurements and Conversions
1000 1000
92 mL = _________ µL 357 µL = _________ mL
0.5 L = _________ mL 0.557 mL = _________ µL
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• Start with 10-mL pipets
• Show it can be read up or down
• Show zero, then to tip
• Explain “TD”
• Demo green pump and drawing up and dispensing
• Add 5.60 mL of red solution to tube #1
• Add 2.80 mL of red solution and 2.80 mL H20 to tube #2, cap, mix
• Compare tubes with other techs
• Use the spec (at 400 nm) to compare them
Measuring Small Volumes with Pipets
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• Repeat with 5-mL pipets
• Show it can be read up or down
• Show zero, then to tip
• Use green pump
• Using 13x100 mm, add 3.60 mL of red solution to tube #3
• Add 1.80 mL of red solution and 1.80 mL H20 to tube #4, cap, mix
• Compare tubes with other techs
• Use the spec (at 400 nm) to compare them
Measuring Small Volumes with Pipets
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• Repeat with 2-mL pipets
• Show it can be read up or down
• Show zero, then to tip
• Use blue pump
• Using 13x100 mm, add 1.90 mL of red solution and 1.90 mL H20 to tube #5, cap, mix
• Using 13x100 mm, add 0.95 mL of red solution and 2.85 mL H20 to tube #5, cap, mix
• Compare tubes with other techs
• Use the spec (at 400 nm) to compare them
Measuring Small Volumes with Pipets
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• Repeat with 1-mL pipets
• Show it can be read up or down
• Show zero, then to tip
• Use blue pump
• Using 13x100 mm, add 0.90 mL of red solution and 2.70 mL H20 to tube #5, cap, mix
• Using 13x100 mm, add 0.45 mL of red solution and 3.35 mL H20 to tube #5, cap, mix
• Compare tubes with other techs
• Use the spec (at 400 nm) to compare them
Measuring Small Volumes with Pipets
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Measuring Very Small Volumes with
Micropipets
• Start with any of the micropipets
• Show how to determine its size and range
• Show how to hold it so display can be read.
• Demonstrate how to read it
• Demonstrate first and second stop
• Demonstrate how to put a tip on and eject the tip.
• Demonstrate how to take up and dispense a sample.
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Measuring Very Small Volumes with
Micropipets
• Using the P-100 and yellow tips
• Each partner measures 75.2 µL of one dye into their own 1.7 mL tube.
• Demonstrate how to compare tube
• Demonstrate to check it by sucking it up
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Measuring Very Small Volumes with
Micropipets
• Using the P-10 and white tips
• Each partner measures 9.85 µL of one dye into their own 1.7 mL tube.
• Demonstrate how to compare tube
• Demonstrate to check it by sucking it up
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Measuring Very Small Volumes with
Micropipets
• Using the P-1000 and blue tips
• Each partner measures 333 µL of one dye into their own 1.7 mL tube.
• Demonstrate how to compare tube
• Demonstrate to check it by sucking it up
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Micropipeting Secret Code
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Micropipeting Secret Code
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Get help starting or improving a biotech program !
Ellyn DaughertySM Biotech Career Pathway
www.SMBiotech.com
www.BiotechEd.com
www.emcp.com/biotech
www.sargentwelch.com/biotech
Amy NaumVWR Ed/Sargent Welch
www.sargentwelch.com/[email protected] 585-321-9422
Holly AhernVWR Ed/Sargent Welch
www.sargentwelch.com/[email protected] 512-789-3155