fullerene, super anti-oxidant -...
TRANSCRIPT
Fullerene, Super Anti-Oxidant
-Clinical Test Results on Whitening and
Anti-Aging-
August 30th , 2013
Vitamin C60 Bioresearch Corporation Tomoko OHTSU
2
Minimum Knowledge about Fullerene!
1. Fullerene(C60) is a carbon allotrope of carbon, like a diamond.
2. Fullerene(C60) is a Nobel Prize winning material
3. Fullerene(C60) is a super anti-oxidant.
- 172 times as effective as Vitamin C
- stable against light and/or heat
- power is long-lasting (more than 24 hours)
Great Effects of Fullerene
Anti-aging(preventive effects of cell death)
Inflammation Easing
(reducing of a ruddy face)
Anti-Wrinkle
Sunscreen / UV cut Whitening
(Melanin Control)
Sebum Oxidation Control
Pore Tightening
3
Recover
Skin barrier
1. Free Radical Scavenging activity
2. Comparison data with
other anti-oxidant
ingredients
3. Preventive Effect of Fullerene
4
Data of Fullerene Effect
- in vitro Study-
Free Radical Scavenging activity
high
low
Co
ncen
trati
on
of
Fre
e
Rad
ical
Without Fullerene With Fullerene
5
-20
0
20
40
60
80
100
Linoleic acid H2O2 UV rayAn
tio
xid
an
t A
cti
vit
y (
AO
A%
)
Vitamin E Vitamin C Derivative(APS) Fullerene(Radical Sponge)
Radical scavenging activity of Fullerene
6
PV
P/C
60
PV
P/C
60O
PV
P/C
70
PV
P/C
60O
n
cate
chin
vitam
in E
isoflavone
coenzym
e Q
10
querc
etin
cru
cm
in
AP
PS
a-lip
oic
acid
An
tio
xid
an
t
Acti
vit
y(A
OA
%)
0
20
40
60
49.5
35.6
29.1
25.1
17.7 14.8
7.35
An
tio
xid
an
t A
cti
vit
y(A
OA
%)
Comparative testing with other antioxidant
ingredients by β-Catotene method
7
Preventive effect of Fullerene
11 h
ours
late
r
Without Fullerene With
Fullerene
8
9
Clinical Test Results by using Fullerene
contained cosmetics
1. Brightening (Whitening) Effects
(Radical Sponge™ )
2. Effect of Fullerene on the Skin Barrier
(Radical Sponge™)
3. Fullerene is effective against wrinkle
( LipoFullerene™ )
Brightening (Whitening) Effects
by Fullerene
Effectively eliminate UV-induced free
radicals
Prevent excessive melanin production
Verified by various clinical trials
10
Suppression of Melanin production by Fullerene
Without Fullerene With Fullerene
Let’s check the movie!!
11
Me
lan
in In
de
x
0
0.5
1
1.5
0 4 8 (weeks)
He
mo
glo
bin
In
de
x
p < 0.001
p < 0.001
0 4 8 (weeks)
1.3
1.2
1.1
1.0
0.9
0.8
0.7
0.6
p < 0.001
Brightening (Whitening) Effect by Fullerene
Subjects: 18 females aged 34 to 49 Area: Face
The melanin index decreased intentionally on the 4 weeks.
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You can feel the Whitening/Brightening effect in about 1-2 month.
Continuous use of Fullerene contained product is the key to have better
results.
After 5 month
After 3 month
Continuous use of Fullerene contained product will lead you
more brighter and fair skin condition.
Brightening (Whitening) Effect by Fullerene
14
Effect of Fullerene on the Skin Barrier
by Fullerene
Increase “Transglutaminase 1” mRNA
expression.
Increase “Cornified Emvelope” formation and
help maintain Skin barrier function.
Clinical study: Help in recovering skin barrier
function.
15
Structure and function of the stratum corneum
Intercellular lipid
Stra
tum
co
rne
um
20 μ
m
Gra
nu
lar la
ye
r Normal stratum corneum
Cornified
cell
Cornified
envelope
(CE)
Moisture loss
Stratum corneum with decreased
skin barrier function
flecks
wrinkles
dullnes
s
rough
skin
dry
skin
・drying
・UV
・stress
・aging
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Clinical Test :
Tape Stripping Method to measure Trans Epidermal Water
Loss (TEWL) Radical Sponge® helped recovery of TEWL on 2nd and 3rd day
significantly
-40
-20
0
20
40
60
80
100
0 1 2 3 4
Rate
of
reco
very
(%)
#P<0.05 vs. RS(0%) # #
RS(0%
)
RS(1%)
days
0 1 2 3
4 Trial Design Randomized blinded study
Method TEWL changes after Tape Stripping
Subjects 10 healthy subjects (5male 5female)
Trail period and applications Solution was applied twice daily for 5days
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Fullerene is effective against wrinkle
by Fullerene
Remove the Free Radicals which is the
primary cause of wrinkles.
Skin structure is improved steadily.
Fullerene/squalene cream significantly
reduced wrinkle area compared to the
placebo-treated area.
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Wrinkles formation by ultraviolet rays
Decrease of MMP9 and FOS mRNA means repression of wrinkles formation.
RO
S
UVB Wrinkle
formation
FOS Epidermi
s
Basement
membrane
Dermis
laminin-5
IV type collagen
VII type collagen
The active oxygen which occurred by ultraviolet rays activates FOS
(intracellular signaling) and MMP9 (degradative enzyme of collagen),
and forms wrinkles.
MMP9(Matrix metallopeptidase 9)・・・ degradative enzyme of laminin-5 etc. 19
The percentage of wrinkle area decreases in the
LipoFullerene application group than a Placebo application
group.
Fullerene is effective against wrinkle
by LipoFullerene
20
Guideline
s
Guidelines for Evaluation of Anti-
Wrinkle Products prepared by the
Japanese Cosmetics Science Society
in 2006
Trial
Design A randomized, double-blind test
Trial
Period
Eight weeks (September 24, 2008 ~
November 19th, 2008)
Subjects 23 Japanese women in their 30s to
40s with wrinkle grade 2 to 3.
Test
Samples
1%-LipoFullerene-blended cream
(active) and Lipofullerene free cream
(placebo)
10 x 10 mm
Skin surface replica Shadow image
Wrinkle: few many
Evaluation
Great Effects of Fullerene
Anti-aging(preventive effects of cell death)
Inflammation Easing
(reducing of a ruddy face)
Anti-Wrinkle
Sunscreen / UV cut Whitening
(Melanin Control)
Sebum Oxidation Control
Pores Tighten
21
Moisturizer
(Skin barrier)
22
For Basic Skin Care Products and MORE!
Basic Skin Care Products - Lotion
- Essence/Serum
- Cream
+
1. Color Cosmetics
(BB Cream, CC Cream)
2. Hair care
(Scalp tonic,
Shampoo & Treatment)
3. Beauty Laser care
4. Sun screen
5. Facial Mask
Fullerene Products in
Overseas
Anti Anti - - oxidant Whitening Serum oxidant Whitening Serum
With Fullerene RS With Fullerene RS ™ ™
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0
200
400
600
800
1000
1200
1400
1600
2005 2007 2009 2011 2013
Number of Clinics
Fullerene is famous for Doctor’s Cosmetics
More than 1,500 clinics in Japan has selected our Fullerene cosmetic
ingredients!!
24
2005 Launch Radial Sponge
2007 Entered to Internet Market
2006 Start Selling in Overseas(Korea・Taiwan・USA)
2008 1000 Clinics selected Fullerene
2009 Launch LipoFullerene
2011
For Clinical Doctors
Dr.Brandt (USA)
Dr.Ci labo
DHC
Over 1000 items are selling in the market !!
Felice Towako Cosmetics
NOVA C60(Canada)
MISSHA
(Korea)
マードゥレクス
CBON
アジュバンコスメ
Dr.Perricone(USA)
JMEC
Derfarma
AR
Doctor Products
WithUs
アプロ サイエンス
TUNEMAKERS
Dr.WU(Taiwan)
Dr. Recella
Unicity
St.Clare (Taiwan)
ISA Nox (Korea)
Water-Soluble Fullerene
Oil-Soluble Fullerene
Fullerene Contained Products
25
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Radical Sponge
(water-soluble)
LipoFullerene
(oil-soluble)
Our Products
Trademark (LOGO)
Products which containing more than 1% of our ingredients(Radical Sponge
or LipoFullerene) can put our trademark to their product package and web page. 27
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Marketing Points of Fullerene!!
Nobel Prized Winning Ingredient 172 times as effective as Vitamin C Stable against light(UV) and/or heat Power is long-lasting (more than 24 hours) Abundance of Clinical data Multifunctional ingredient Safety and secure ingredient selected by Clinic Doctors
Samples of our ingredient is available!!
After concluding Non-Disclosure Agreement (Sample
Evaluation Agreement), VC60 will send sample to the
customer for free.
• Radical Sponge (water-soluble Fullerene) : 20 g
• LipoFullerene (oil-soluble Fullerene): 16 g
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Sample formulations are available!!
• Radical Sponge (water-soluble Fullerene)
Serum & Cream
• LipoFullerene (oil-soluble Fullerene):
16 g
Milk Lotion W/O type & O/W type
30
Exhibitions
in-cosmetics Asia 2013
Date: October 29th – 31st, 2013
Time: 10:00 – 17:00
Place: BITEC Hall Bangkok,
Thailand
Booth number: E10
(Booth share with Bronson &
Jacobs)
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Vitamin C60 BioResarch Corporation
Contact:
+81-3-3517-3252
http://www.vc60.com
Thank you for your attention!